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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phHSPB1

Promoter collection, Human HSPB1 promoter

Catalog number RDB07530
Resource name pGL4-phHSPB1
Alternative name U2846-7
Clone info. PCR amplified human HSPB1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 76301177 to 76302661(NC_000007.14); relatively -1496 to -11, where +1 corresponds to 1 nt of NM_001540.5.
Comment PCR cloning, forward primer: GTTGCCCAGGCTTGTTCCAG; reverse primer: CTCGGCTGCGCTTTTATGCT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27

          Reference sequence
            

          Distribution information

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          RDB07530 pGL4-phHSPB1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07530.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07530_A5Dn.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07530_A5Dna.seq check
          >07530_07530_A5Dn_pGL4-4174F_C11_08.ab1
              1 GATGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
             61 TGTTGCCCAG GCTTGTTCCA GCCTTCTGGC TTCAAGTAAT CCTCCCACAT CAGCCTCCCA
            121 AAGTGCTAAG ATTACAGATG TCAGCCACTG CACCCAGCCA GTAATATAAT TTTATGGGAC
            181 CACCTTCATA TTTGCTGTCC CTTGCTGACT TACACATCTT TATGCAATGC ATGACTGTTA
            241 CCATCATTAT CATCTCTATT TTCCAGATGG GGAAACTGAG GCACAAAGAA TCTAACTTGC
            301 ACAAGTTCAT CTGCTTAGTG ATGGAAGAAA GATGTGAATT CAGGCAGTCT GGCTTCAAAG
            361 TCCACACGCC TAACAACCAC ACCAGATTAC TAGATTGCTT TTTTCTTTTT TCTTTTTTTT
            421 TTTTTTGAGA TGGAGTCTCA CTCTGTCACC CAGGCTGGAG TACAGTGGTG AGATCTCGGC
            481 TCACTGCAAC CTCTGCCTTC TGGGTTCAAG CAATTCTCCT GCCTCAGCCT CCCCAGCAGC
            541 TGCGATTACA GGCGCCCGCC ACCACACCCA GCTAATTTTT GTATTTTTAG TAGAGATGGG
            601 GTTTCACCAT GTTGGCCAGG CTGGTCTCAA ACTCCTGACC TCTGGTGATC CTCCCACCTC
            661 GGTCTCCCAA AGTGCTGGGA TTACAGGCGT GAGCCACCAC GCCCAGCCCA GACTGCTTTA
            721 TTTTTGTATT TGTATTTATT CATTTACTTA TTTTGAGACA GGGTTTTGCT CTGTAGCCCA
            781 GGCTGAAGTG CAGTGGTGCA ATCCAGCTCA CCACAGCCTC TACTCACCGG GGTTCAAAGG
            841 ATCCTCCTGC TTCAGCCTCT GGAGTAGCTG GGGCCACAGG CATGCACCAC CATGCCCAGC
            901 TAATTTTTAA ATATTTTTTT GGTAGAAGTA GGGTCTCACT ATGTTGCCCA GACTGGTCTC
            961 AAACTCCTAG CCTCAAAGGG ACCCTTCTGC CCTTGGGCCT CCCAAAGTGC TGAGATTTAC
           1021 AGGCATGAGC CCATGCACCC CAGCCCCTTT TTTAAAATTT TTTTTTGAGA GACAAGACCT
           1081 TTGATCTGCT GCTAGCTGGA GTGCAATTGG TTGAGATCAT AGCTCACTGC AAGCTTCGAC
          1141 TTCTGGTTCA GCACAGACTT CGATTAAAAT TGA
          //
          Primer: pGL4-136R
          Sequence file: RDB07530_A5Dnb.seq check
          >07530_07530_A5Dn_pGL4-136R_D11_11.ab1
              1 GTCTAGCTTT GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
             61 CGCCGAGGCC AGATCTTGAT CTCGGCTGCG CTTTTATGCT CCTCCAGTCG GGTATTTTTA
            121 GCAGGCGGTG TTTGAGGTCT CTATTAATGG CAATGACCCG TTTGAGGGCC GCCCCTCCCC
            181 ATGCACTCCT CCCCCAGCGT TCAGGGGCCG TGGGCGGGGC GAAGAGGGTT CAGCCCTCAT
            241 CTGGAACCTT CTCTCGTTAA GGAAAGCAAA TGAATTCGAG AGCGCGACGC TGGAGCCGTG
            301 CCACGGCAGG GCACTCGCCG GCCCTGGTGC TTAAGCGTTG CTGGCTGTGG CCCGTGGCCA
            361 GGGCCGTCCT TCTGTTAAGG AGGACAGAGC CAGACAGGTT GGGTTGGGGC ATACAACTGA
            421 GAAGTGGCAG AGGCAGGATT GGAACCACAC GCGTGTGAGA TAGAATGTGA TAAAAGGAGT
            481 CTGGTGCTTG AGCCCAGGAG TTGAGGCTGC AGTGAGCTAT GATCTCACCA CTGCACTCCA
            541 GCCTAGGCAA CAGATCAAAG TCTTGTCTCT CAAAAAAATT TTAAAAAGGG GCTGGGTGCA
            601 TGGCTCATGC CTGTAATCTC AGCACTTTGG GAGGCCAAGG CAGAAGGGTC CCTTGAGGCT
            661 AGGAGTTTGA GACCAGTCTG GGCAACATAG TGAGACCCTA CTTCTACCAA AAAATATTTA
            721 AAAATTAGCT GGGCATGGTG GTGCATGCCT GTGGCCCCAG CTACTCCAGA GGCTGAAGCA
            781 GGAGGATCCT TTGAACCCCG GTGAGTAGAG GCTGTGGTGA GCTGGATTGC ACCACTGCAC
            841 TTCAGCCTGG GGCTACAGAG CAAAACCCTG TCTCAAAATA AGTAAATGAA TAAATACAAA
            901 TACAAAAATA AAGCAGTCTG GGCTGGGCGT GGTGGCTCAC GCCTGTAATC CCAGCACTTT
            961 GGGAGACCGA GGTGGGAGGA TCACCAGAGG TCAGGAGTTT GAGACCAGCC CTGGCCAACA
           1021 TGGGTGAAAC CCCCATCTCT ACTAAAAATA CAAAAATTAG CTGGGATGTG GTGGCCGGGC
           1081 CGTCTGGTAT TCGCAAGCCT GGCTGGGGAG GCTGCAGGTC AGGCAAGAAA TTGGCCTTGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl