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pGL4-phHSPB1 (#RDB07530)

Promoter collection, Human HSPB1 promoter

Alternative name U2846-7
Clone info. PCR amplified human HSPB1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 76301177 to 76302661(NC_000007.14); relatively -1496 to -11, where +1 corresponds to 1 nt of NM_001540.5.
Comment PCR cloning, forward primer: GTTGCCCAGGCTTGTTCCAG; reverse primer: CTCGGCTGCGCTTTTATGCT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07530.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07530 pGL4-phHSPB1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phHSPB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07530).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07530_A5Dn.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07530_A5Dna.seq check
>07530_07530_A5Dn_pGL4-4174F_C11_08.ab1
    1 GATGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
   61 TGTTGCCCAG GCTTGTTCCA GCCTTCTGGC TTCAAGTAAT CCTCCCACAT CAGCCTCCCA
  121 AAGTGCTAAG ATTACAGATG TCAGCCACTG CACCCAGCCA GTAATATAAT TTTATGGGAC
  181 CACCTTCATA TTTGCTGTCC CTTGCTGACT TACACATCTT TATGCAATGC ATGACTGTTA
  241 CCATCATTAT CATCTCTATT TTCCAGATGG GGAAACTGAG GCACAAAGAA TCTAACTTGC
  301 ACAAGTTCAT CTGCTTAGTG ATGGAAGAAA GATGTGAATT CAGGCAGTCT GGCTTCAAAG
  361 TCCACACGCC TAACAACCAC ACCAGATTAC TAGATTGCTT TTTTCTTTTT TCTTTTTTTT
  421 TTTTTTGAGA TGGAGTCTCA CTCTGTCACC CAGGCTGGAG TACAGTGGTG AGATCTCGGC
  481 TCACTGCAAC CTCTGCCTTC TGGGTTCAAG CAATTCTCCT GCCTCAGCCT CCCCAGCAGC
  541 TGCGATTACA GGCGCCCGCC ACCACACCCA GCTAATTTTT GTATTTTTAG TAGAGATGGG
  601 GTTTCACCAT GTTGGCCAGG CTGGTCTCAA ACTCCTGACC TCTGGTGATC CTCCCACCTC
  661 GGTCTCCCAA AGTGCTGGGA TTACAGGCGT GAGCCACCAC GCCCAGCCCA GACTGCTTTA
  721 TTTTTGTATT TGTATTTATT CATTTACTTA TTTTGAGACA GGGTTTTGCT CTGTAGCCCA
  781 GGCTGAAGTG CAGTGGTGCA ATCCAGCTCA CCACAGCCTC TACTCACCGG GGTTCAAAGG
  841 ATCCTCCTGC TTCAGCCTCT GGAGTAGCTG GGGCCACAGG CATGCACCAC CATGCCCAGC
  901 TAATTTTTAA ATATTTTTTT GGTAGAAGTA GGGTCTCACT ATGTTGCCCA GACTGGTCTC
  961 AAACTCCTAG CCTCAAAGGG ACCCTTCTGC CCTTGGGCCT CCCAAAGTGC TGAGATTTAC
 1021 AGGCATGAGC CCATGCACCC CAGCCCCTTT TTTAAAATTT TTTTTTGAGA GACAAGACCT
 1081 TTGATCTGCT GCTAGCTGGA GTGCAATTGG TTGAGATCAT AGCTCACTGC AAGCTTCGAC
1141 TTCTGGTTCA GCACAGACTT CGATTAAAAT TGA
//
Primer: pGL4-136R
Sequence file: RDB07530_A5Dnb.seq check
>07530_07530_A5Dn_pGL4-136R_D11_11.ab1
    1 GTCTAGCTTT GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
   61 CGCCGAGGCC AGATCTTGAT CTCGGCTGCG CTTTTATGCT CCTCCAGTCG GGTATTTTTA
  121 GCAGGCGGTG TTTGAGGTCT CTATTAATGG CAATGACCCG TTTGAGGGCC GCCCCTCCCC
  181 ATGCACTCCT CCCCCAGCGT TCAGGGGCCG TGGGCGGGGC GAAGAGGGTT CAGCCCTCAT
  241 CTGGAACCTT CTCTCGTTAA GGAAAGCAAA TGAATTCGAG AGCGCGACGC TGGAGCCGTG
  301 CCACGGCAGG GCACTCGCCG GCCCTGGTGC TTAAGCGTTG CTGGCTGTGG CCCGTGGCCA
  361 GGGCCGTCCT TCTGTTAAGG AGGACAGAGC CAGACAGGTT GGGTTGGGGC ATACAACTGA
  421 GAAGTGGCAG AGGCAGGATT GGAACCACAC GCGTGTGAGA TAGAATGTGA TAAAAGGAGT
  481 CTGGTGCTTG AGCCCAGGAG TTGAGGCTGC AGTGAGCTAT GATCTCACCA CTGCACTCCA
  541 GCCTAGGCAA CAGATCAAAG TCTTGTCTCT CAAAAAAATT TTAAAAAGGG GCTGGGTGCA
  601 TGGCTCATGC CTGTAATCTC AGCACTTTGG GAGGCCAAGG CAGAAGGGTC CCTTGAGGCT
  661 AGGAGTTTGA GACCAGTCTG GGCAACATAG TGAGACCCTA CTTCTACCAA AAAATATTTA
  721 AAAATTAGCT GGGCATGGTG GTGCATGCCT GTGGCCCCAG CTACTCCAGA GGCTGAAGCA
  781 GGAGGATCCT TTGAACCCCG GTGAGTAGAG GCTGTGGTGA GCTGGATTGC ACCACTGCAC
  841 TTCAGCCTGG GGCTACAGAG CAAAACCCTG TCTCAAAATA AGTAAATGAA TAAATACAAA
  901 TACAAAAATA AAGCAGTCTG GGCTGGGCGT GGTGGCTCAC GCCTGTAATC CCAGCACTTT
  961 GGGAGACCGA GGTGGGAGGA TCACCAGAGG TCAGGAGTTT GAGACCAGCC CTGGCCAACA
 1021 TGGGTGAAAC CCCCATCTCT ACTAAAAATA CAAAAATTAG CTGGGATGTG GTGGCCGGGC
 1081 CGTCTGGTAT TCGCAAGCCT GGCTGGGGAG GCTGCAGGTC AGGCAAGAAA TTGGCCTTGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles