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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phGADD45B

Promoter collection, Human GADD45B promoter

Catalog number RDB07529
Resource name pGL4-phGADD45B
Alternative name U2845-7
Clone info. PCR amplified human GADD45B promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 2474664 to 2476169(NC_000019.10); relatively -1463 to +43, where +1 corresponds to 1 nt of NM_015675.4.
Comment PCR cloning, forward primer: CCCGTCCGTCCCTCTCTTC; reverse primer: GGAAGTTGCGGAAACCAACG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human DKFZp566B133, GADD45BETA, MYD118 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human DKFZp566B133, GADD45BETA, MYD118

          Reference sequence
            

          Distribution information

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          RDB07529 pGL4-phGADD45B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07529.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07529_A5Bn.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07529_A5Bna.seq check
          >07529_07529_A5Bn_pGL4-4174F_F03_18.ab1
              1 AGCAGGGTCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
             61 ATCCCGTCCG TCCCTCTCTT CCGGCGCTGA GGGCGCAGAA ACTCGGCGCG GCCACCAGAT
            121 GGCGCCACCG CGCCTCGCAG CCCCGGGACA TTTCGTGCCC CAGCGGTGGG CGGCGCGGAG
            181 GAGGTGGGAT GCGTCAGCCG GACCCGCGGG CCGGGGAGCC GGGCAACTGG GGAAAGTGAG
            241 CCGCCGGGCG GAGGCCAGAC CCCAGGCTGG CGGGGGAGGG GACTCAGATG TGAGTCACCC
            301 GGGAGCCCCC AGTCGACCGT GGGAGACGTT GTCATGCGGG GCCTGCCAGC CTCGCTCCTG
            361 AACAGCCTTC CCGGGGCGGC GGAGTCCTGC AGGCGGCCGT GCCTGGCGCA CACGCGTGGC
            421 TCCAACACGA ACTAGCCTAT GGCCTCACCA AGCCTCAGTT TCCCCTTTTG CATAATGCAC
            481 ATAAGCATCC CCCCTGCTTC CCAGGTCTTG GATTAAATCA TCCATGAATC CGGAGGGGTG
            541 GGGGGTACCA GGTCCGCACC ACGCACTCAA TGCAGGTCAG CGCCCACCCC GGAGTGAGAA
            601 ATAAAAGTAG CCGTCATCCT TGCACGTGGG CCCTGGGTGG CGGCGTGCAG CCGGAGCCTG
            661 CACCATTCCC CTCAACAATG GCTTTCTCCC GGGCCTGGAG GCCTCCGCAC GGGCAAAGGC
            721 TGGAACGCCA GGCAGCCGGC GGGGGCGGGG CCGCGCCACC TGCACCTCCC ACGCGCACCC
            781 CACCCTGATA CTCGCGGGGT CGGCGAGGAG GCGCCCACTT TCTTCTCCAG AGCGCCCCGG
            841 GCTTTGCAAC CTCCCACTTT CGGGGCCCTC GAGGAGGCCT CCCGCGACCA CCAGGTGACA
            901 GCTGATGTGT ATTGGGCTCT TACTGTCAGC CGTATTTTAT GCCATGCTCT GCAAACCAGC
            961 GAGGCCGGCG CTGCAGACCC ATTACTCAGA CGGGAACAGA GAGGCCCGGG AGAAGCGAAA
           1021 TCACCCAGGG GCTGGGGGTC GTCGCAGCCA GGAGAGACTC CGGCCCTCAC CACCAACTGG
           1081 GCGAGATCAC GCTGCAAACG GGATCCGATC CCGATGCAGC CCCTTCCAAC CCCAAGCCAG
          1141 AACTTGGGTA AAGGCGCGCG CGG
          //
          Primer: pGL4-136R
          Sequence file: RDB07529_A5Bnb.seq check
          >07529_07529_A5Bn_pGL4-136R_G03_21.ab1
              1 GTCTTTTGCT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
             61 GGCCGCCGAG GCCAGATCTT GATGGAAGTT GCGGAAACCA ACGGTAGTCC GACGCTTCGG
            121 CGATCTGAGC TGGGAGCCTC CGGCACCGAC TTTTATAGAC TTGAACATTG GGCGTGGCCT
            181 CAGTGGAGTC ATTCGCCCCT CCTATTGGTG GAAAGACAAG GGCGAGTGCA GCTAGCTCTG
            241 CGGTTGGCCG ACGGAGGGGA GGGGGAGGAG CGGCGTGCGG GGGCGCCCCA CGTGGGGGCG
            301 AGAACCGGGA GGAGGGCGCG CGGGGGCCGG GTAGGTGACC GCGAATGCCA GAAAAGAAAA
            361 AAAAGCAGAA GTTTTTTTCT CCTGATTTCC CGGCAGCCGA GCCGCGGTTC GGGGCGCGCG
            421 CGGGATTTCC AGCGCGCGAG GCGGCCCGGA CTTTCGGGGG GGCCTGGAAT CCCCTCCCGA
            481 TCCGCGGAGA GTCCCGGACC GCGCCTTTCC CAAGTTCTGC TGGGGGTGGA GGGGCTGCAC
            541 CGGGAAGGGG CCCCGTTTGC AGCGTGATCT CGCCCAGGTG GTGGTGAGGG CCGGAGTCTC
            601 TCCTGGCTGC GACGACCCCA GCCCCTGGGT GATTTCGCTT CTCCCGGCCT CTCTGTTCCC
            661 GTCTGAGTAA TGGGTCTGCA GCGCCGGCCT CGCTGGTTTG CAGAGCATGG CATAAAATAC
            721 GGCTGACAGT AAGAGCCCAA TACACATCAG CTGTCACCTG GTGGTCGCGG GAGGCCTCCT
            781 CGAGGGCCCC GAAAGTGGGA GGTTGCAAAG CCCGGGGCGC TCTGGAGAAG AAAGTGGGCG
            841 CCTCCTCGCC GACCCCGCGA GTATCACGGG TGGGGTGCGC GTGGGAGGTG CAGGTGGCGC
            901 GGCCCCGCCC CCGCCGGCTG CCTGGCGTTC CAGCCTTTGC CCGTGCGGAG GCCTCCAGGC
            961 CCGGGAGAAG CCATTGTTGA GGGGAATGTG CAGCTCCGGC TGCACGCCGC CACCCAGGCC
           1021 CACGTGCAGG ATGACGGCTA CTTTTATTTC TCACTCCGGG GTGGCGCTGA ACTTGCAATT
           1081 GATTGCCTTG TGCCGAACTG GTACCCTCAT CCTTCCGGAA TCCATGATGA ATTAATTCCA
          1141 AGACCTTGGG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl