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pGL4-phGADD45B (#RDB07529)

Promoter collection, Human GADD45B promoter

Alternative name U2845-7
Clone info. PCR amplified human GADD45B promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 2474664 to 2476169(NC_000019.10); relatively -1463 to +43, where +1 corresponds to 1 nt of NM_015675.4.
Comment PCR cloning, forward primer: CCCGTCCGTCCCTCTCTTC; reverse primer: GGAAGTTGCGGAAACCAACG. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human DKFZp566B133, GADD45BETA, MYD118 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07529.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07529 pGL4-phGADD45B DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phGADD45B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07529).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07529_A5Bn.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07529_A5Bna.seq check
>07529_07529_A5Bn_pGL4-4174F_F03_18.ab1
    1 AGCAGGGTCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
   61 ATCCCGTCCG TCCCTCTCTT CCGGCGCTGA GGGCGCAGAA ACTCGGCGCG GCCACCAGAT
  121 GGCGCCACCG CGCCTCGCAG CCCCGGGACA TTTCGTGCCC CAGCGGTGGG CGGCGCGGAG
  181 GAGGTGGGAT GCGTCAGCCG GACCCGCGGG CCGGGGAGCC GGGCAACTGG GGAAAGTGAG
  241 CCGCCGGGCG GAGGCCAGAC CCCAGGCTGG CGGGGGAGGG GACTCAGATG TGAGTCACCC
  301 GGGAGCCCCC AGTCGACCGT GGGAGACGTT GTCATGCGGG GCCTGCCAGC CTCGCTCCTG
  361 AACAGCCTTC CCGGGGCGGC GGAGTCCTGC AGGCGGCCGT GCCTGGCGCA CACGCGTGGC
  421 TCCAACACGA ACTAGCCTAT GGCCTCACCA AGCCTCAGTT TCCCCTTTTG CATAATGCAC
  481 ATAAGCATCC CCCCTGCTTC CCAGGTCTTG GATTAAATCA TCCATGAATC CGGAGGGGTG
  541 GGGGGTACCA GGTCCGCACC ACGCACTCAA TGCAGGTCAG CGCCCACCCC GGAGTGAGAA
  601 ATAAAAGTAG CCGTCATCCT TGCACGTGGG CCCTGGGTGG CGGCGTGCAG CCGGAGCCTG
  661 CACCATTCCC CTCAACAATG GCTTTCTCCC GGGCCTGGAG GCCTCCGCAC GGGCAAAGGC
  721 TGGAACGCCA GGCAGCCGGC GGGGGCGGGG CCGCGCCACC TGCACCTCCC ACGCGCACCC
  781 CACCCTGATA CTCGCGGGGT CGGCGAGGAG GCGCCCACTT TCTTCTCCAG AGCGCCCCGG
  841 GCTTTGCAAC CTCCCACTTT CGGGGCCCTC GAGGAGGCCT CCCGCGACCA CCAGGTGACA
  901 GCTGATGTGT ATTGGGCTCT TACTGTCAGC CGTATTTTAT GCCATGCTCT GCAAACCAGC
  961 GAGGCCGGCG CTGCAGACCC ATTACTCAGA CGGGAACAGA GAGGCCCGGG AGAAGCGAAA
 1021 TCACCCAGGG GCTGGGGGTC GTCGCAGCCA GGAGAGACTC CGGCCCTCAC CACCAACTGG
 1081 GCGAGATCAC GCTGCAAACG GGATCCGATC CCGATGCAGC CCCTTCCAAC CCCAAGCCAG
1141 AACTTGGGTA AAGGCGCGCG CGG
//
Primer: pGL4-136R
Sequence file: RDB07529_A5Bnb.seq check
>07529_07529_A5Bn_pGL4-136R_G03_21.ab1
    1 GTCTTTTGCT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
   61 GGCCGCCGAG GCCAGATCTT GATGGAAGTT GCGGAAACCA ACGGTAGTCC GACGCTTCGG
  121 CGATCTGAGC TGGGAGCCTC CGGCACCGAC TTTTATAGAC TTGAACATTG GGCGTGGCCT
  181 CAGTGGAGTC ATTCGCCCCT CCTATTGGTG GAAAGACAAG GGCGAGTGCA GCTAGCTCTG
  241 CGGTTGGCCG ACGGAGGGGA GGGGGAGGAG CGGCGTGCGG GGGCGCCCCA CGTGGGGGCG
  301 AGAACCGGGA GGAGGGCGCG CGGGGGCCGG GTAGGTGACC GCGAATGCCA GAAAAGAAAA
  361 AAAAGCAGAA GTTTTTTTCT CCTGATTTCC CGGCAGCCGA GCCGCGGTTC GGGGCGCGCG
  421 CGGGATTTCC AGCGCGCGAG GCGGCCCGGA CTTTCGGGGG GGCCTGGAAT CCCCTCCCGA
  481 TCCGCGGAGA GTCCCGGACC GCGCCTTTCC CAAGTTCTGC TGGGGGTGGA GGGGCTGCAC
  541 CGGGAAGGGG CCCCGTTTGC AGCGTGATCT CGCCCAGGTG GTGGTGAGGG CCGGAGTCTC
  601 TCCTGGCTGC GACGACCCCA GCCCCTGGGT GATTTCGCTT CTCCCGGCCT CTCTGTTCCC
  661 GTCTGAGTAA TGGGTCTGCA GCGCCGGCCT CGCTGGTTTG CAGAGCATGG CATAAAATAC
  721 GGCTGACAGT AAGAGCCCAA TACACATCAG CTGTCACCTG GTGGTCGCGG GAGGCCTCCT
  781 CGAGGGCCCC GAAAGTGGGA GGTTGCAAAG CCCGGGGCGC TCTGGAGAAG AAAGTGGGCG
  841 CCTCCTCGCC GACCCCGCGA GTATCACGGG TGGGGTGCGC GTGGGAGGTG CAGGTGGCGC
  901 GGCCCCGCCC CCGCCGGCTG CCTGGCGTTC CAGCCTTTGC CCGTGCGGAG GCCTCCAGGC
  961 CCGGGAGAAG CCATTGTTGA GGGGAATGTG CAGCTCCGGC TGCACGCCGC CACCCAGGCC
 1021 CACGTGCAGG ATGACGGCTA CTTTTATTTC TCACTCCGGG GTGGCGCTGA ACTTGCAATT
 1081 GATTGCCTTG TGCCGAACTG GTACCCTCAT CCTTCCGGAA TCCATGATGA ATTAATTCCA
1141 AGACCTTGGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles