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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phESR1

Promoter collection, Human ESR1 promoter

Catalog number RDB07528
Resource name pGL4-phESR1
Alternative name U2843-3
Clone info. PCR amplified human ESR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 151806130 to 151807567(NC_000006.12); relatively -1549 to -111, where +1 corresponds to 1 nt of NM_000125.3; relatively -1189 to +249, where +1 corresponds to 1 nt of NM_001122740.1.
Comment PCR cloning, forward primer: TTGCTGGCCATAAAGGGAAA; reverse primer: CACTCCAGGCACAACTCGAT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.  2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07528 pGL4-phESR1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07528.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0002j (Japanese text)
          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12848_A4Im.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB12848_A4Ima.seq check
          >07528_12848_A4Im_pGL4-4174F_F07_16.ab1
              1 AATGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
             61 TTTGCTGGCC ATAAAGGGAA ATATCTGCGA TGCACCTAAT GTGTTTTTAA CCCTTTATTT
            121 GCTGACAATC TATAGTCATT AATGCTAAAC TCGATTTTGG CTTCAGCTAC ATTTGCATAT
            181 TGTCCAACAA TGGTCTATTT TTGTAAGAAT TAGATAAAAT GTATACTTGA TATAAAATAG
            241 TCAAAAATGT AACTCTTAGT AACAGTAAGC TTGGCATTTA GATAGACCAT GAACACTTCG
            301 TCAGATACTC TGTTGGGTGT TTGGGATAGC AATTAAAACA AAGTATTGAT AGTTGTATCA
            361 GAGTCTATTA GGCTGCAGCA AAGGAAGTTT ATTCAAAAGT ATAAACTATC CAAGATTATA
            421 GACGCATGAT ATACTTCACC TATTTTTTGT CTCCTTAATA TGTATATATA TATATATATA
            481 TATATATATA TACACATATA TGTGTGTGTG TATGTGCGTG TGCATGTTTA ACTTTTAATT
            541 CAGTTAAAAA CTTTTTTCTA TTTGTTTTTC ATCTGGATAT TTGATTCTGC ATATCCTAGC
            601 CCAAGTGAAC CGAGAAGATC GAGTTGTAGG ACTAAAGGAT AGACATGCAG AAATGCATTT
            661 TAAAAATCTG TTAGCTGGAC CAGACCGACA ATGTAACATA ATTGCCAAAG CTTTGGTTCG
            721 TGACCTGAGG TTATGTTTGG TATGAAAAGG TCACATTTTA TATTCAGTTT TCTGAAGTTT
            781 TGGTTGCATA ACCAACCTGT GGAAGGCATG AACACCCATG TGCGCCCTAA CCAAAGGTTT
            841 TTCTGAATCA TCCTTCACAT GAGAATTCCT AATGGGACCA AGTACAGTAC TGTGGTCCAA
            901 CATAAACACA CAAGTCAGGC TGAGAGAATC TCAGAAGGTT GTGGAAGGGT CTATCTACTT
            961 TGGGAGCATT TTGCAGAGGA AGAAACTGAG GTCCTGGCAG GTTGCATTCT CCTGATGGCA
           1021 AAATGCAGCT CTTCCTATAT GTATACCCTG AATCTCCGCC CCCCTTTCCC CTCAGAATGC
           1081 CCCCTGTCAG TTTCCCCCCA GCTGCTTAAA TATAGCCTGT CTGTTGCTGA CTTGCGTATG
          1141 GCAACCGCAA CCAACCCCCA ATCCTATCCT GC
          //
          Primer: pGL4-136R
          Sequence file: RDB12848_A4Imb.seq check
          >07528_12848_A4Im_pGL4-136R_G07_19.ab1
              1 GCTTTCTTTG TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT
             61 TGGCCGCCGA GGCCAGATCT TGATCACTCC AGGCACAACT CGATTTGGAG CGATCCCAAA
            121 GAGCAGCTTC CCTGAACTTT ACTTTACTTG TCGTCGCTGC TGGATAGAGG CTGAGTTTCA
            181 CGGCCAGGGG GCGGGGGCGC ACGAGGATCT GCTAAAGGTG GCCCAGGGAA GACTGGGCTT
            241 AAAATAAACG CGAAAGACGG ATCCAGGGGC CGGCTTTCTC TAATGTGCTG CCTTATGTGC
            301 CCCGTGCCAG ACTCCAATAT ATCTCTGTTT GTCTCTCTTT CTGTTTGATT CCCCCTCCTC
            361 GTTGGCTAGA AATACGTAGT GTGTACATAG GATGACCCTG GGGAGGACTA CACTGTAACC
            421 GAGATAGGGC AGATAGAATG GGGTGTGCGG TTGCATACGC AGCCAGCCAC AGACAGCTAT
            481 ATTTAGCAGC TGGGGGAACT GACAGGGGGC ATCTGAGGGG AAGGGGGCGG AGATTCAGGG
            541 TATACATATA GGAAGAGCTG CATTTTGCCA TCAGGAGAAT GCAACCTGCC AGGACCTCAG
            601 TTTCTTCCTC TGCAAAATGC TCCCAAAGTA GATAGACCCT TCCACAACCT TCTGAGATTC
            661 TCTCAGCCTG ACTTGTGTGT TTATGTTGGA CCACAGTACT GTACTTGGTC CCATTAGGAA
            721 TTCTCATGTG AAGGATGATT CAGAAAAACC TTTGGTTAGG GCGCACATGG GTGTTCATGC
            781 CTTCCACAGG TTGGTTATGC AACCAAAACT TCAGAAAACT GAATATAAAA TGTGACCTTT
            841 TCATACCAAA CATAACCTCA GTCACGAACC AAAGCTTTGG CAATTATGTT ACATTGTCGG
            901 TCTGGTCCAG CTAACAGATT TTTAAAATGC ATTTCTGCAT GTCTATCCTT TAGTCCTACA
            961 CTCGATCTTC TCGGTTCCCT TGGGCTAGAT ATGCAGATCA AATATCCAGA TGAACATAGA
           1021 GAGTTTTACT GATAAGTAAA CATGCAACGC CCATACACAC CACATAATGT GCATTAATCT
           1081 AATAATATAT AATATTACGA TTAGGATAAA ATAGTGAAGT AATCATGCGC TCTATATCCT
          1141 T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Chiu, J.H., Screening to identify commonly used chinese herbs that affect ERBB2 and ESR1 gene expression using the human breast cancer MCF-7 cell line. Evid. Based Complement. Alternat. Med., 965486 (2014). PMID 24987437.
          user_report Noomhorm, N., In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen. J. Pharmacol. Sci. 125 (4): 375-385 (2014). PMID 25141924.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl