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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phESR1

Promoter collection, Human ESR1 promoter

Catalog number RDB07528
Resource name pGL4-phESR1
Alternative name U2843-3
Clone info. PCR amplified human ESR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 151806130 to 151807567(NC_000006.12); relatively -1549 to -111, where +1 corresponds to 1 nt of NM_000125.3; relatively -1189 to +249, where +1 corresponds to 1 nt of NM_001122740.1.
PCR cloning, forward primer: TTGCTGGCCATAAAGGGAAA; reverse primer: CACTCCAGGCACAACTCGAT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 genomic DNA
Depositor|Developer DNA Bank, |
 
Other clones in our bank human ESR1 (NCBI Gene 2099) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.  2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07528 pGL4-phESR1 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Remarks, protocol and/or map (pdf) RDB07528.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0002j (Japanese text)
Featured content Promoter Collection (Firefly Luciferase) (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12848_A4Im.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB12848_A4Ima.seq check
>07528_12848_A4Im_pGL4-4174F_F07_16.ab1
    1 AATGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
   61 TTTGCTGGCC ATAAAGGGAA ATATCTGCGA TGCACCTAAT GTGTTTTTAA CCCTTTATTT
  121 GCTGACAATC TATAGTCATT AATGCTAAAC TCGATTTTGG CTTCAGCTAC ATTTGCATAT
  181 TGTCCAACAA TGGTCTATTT TTGTAAGAAT TAGATAAAAT GTATACTTGA TATAAAATAG
  241 TCAAAAATGT AACTCTTAGT AACAGTAAGC TTGGCATTTA GATAGACCAT GAACACTTCG
  301 TCAGATACTC TGTTGGGTGT TTGGGATAGC AATTAAAACA AAGTATTGAT AGTTGTATCA
  361 GAGTCTATTA GGCTGCAGCA AAGGAAGTTT ATTCAAAAGT ATAAACTATC CAAGATTATA
  421 GACGCATGAT ATACTTCACC TATTTTTTGT CTCCTTAATA TGTATATATA TATATATATA
  481 TATATATATA TACACATATA TGTGTGTGTG TATGTGCGTG TGCATGTTTA ACTTTTAATT
  541 CAGTTAAAAA CTTTTTTCTA TTTGTTTTTC ATCTGGATAT TTGATTCTGC ATATCCTAGC
  601 CCAAGTGAAC CGAGAAGATC GAGTTGTAGG ACTAAAGGAT AGACATGCAG AAATGCATTT
  661 TAAAAATCTG TTAGCTGGAC CAGACCGACA ATGTAACATA ATTGCCAAAG CTTTGGTTCG
  721 TGACCTGAGG TTATGTTTGG TATGAAAAGG TCACATTTTA TATTCAGTTT TCTGAAGTTT
  781 TGGTTGCATA ACCAACCTGT GGAAGGCATG AACACCCATG TGCGCCCTAA CCAAAGGTTT
  841 TTCTGAATCA TCCTTCACAT GAGAATTCCT AATGGGACCA AGTACAGTAC TGTGGTCCAA
  901 CATAAACACA CAAGTCAGGC TGAGAGAATC TCAGAAGGTT GTGGAAGGGT CTATCTACTT
  961 TGGGAGCATT TTGCAGAGGA AGAAACTGAG GTCCTGGCAG GTTGCATTCT CCTGATGGCA
 1021 AAATGCAGCT CTTCCTATAT GTATACCCTG AATCTCCGCC CCCCTTTCCC CTCAGAATGC
 1081 CCCCTGTCAG TTTCCCCCCA GCTGCTTAAA TATAGCCTGT CTGTTGCTGA CTTGCGTATG
1141 GCAACCGCAA CCAACCCCCA ATCCTATCCT GC
//
Primer: pGL4-136R
Sequence file: RDB12848_A4Imb.seq check
>07528_12848_A4Im_pGL4-136R_G07_19.ab1
    1 GCTTTCTTTG TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT
   61 TGGCCGCCGA GGCCAGATCT TGATCACTCC AGGCACAACT CGATTTGGAG CGATCCCAAA
  121 GAGCAGCTTC CCTGAACTTT ACTTTACTTG TCGTCGCTGC TGGATAGAGG CTGAGTTTCA
  181 CGGCCAGGGG GCGGGGGCGC ACGAGGATCT GCTAAAGGTG GCCCAGGGAA GACTGGGCTT
  241 AAAATAAACG CGAAAGACGG ATCCAGGGGC CGGCTTTCTC TAATGTGCTG CCTTATGTGC
  301 CCCGTGCCAG ACTCCAATAT ATCTCTGTTT GTCTCTCTTT CTGTTTGATT CCCCCTCCTC
  361 GTTGGCTAGA AATACGTAGT GTGTACATAG GATGACCCTG GGGAGGACTA CACTGTAACC
  421 GAGATAGGGC AGATAGAATG GGGTGTGCGG TTGCATACGC AGCCAGCCAC AGACAGCTAT
  481 ATTTAGCAGC TGGGGGAACT GACAGGGGGC ATCTGAGGGG AAGGGGGCGG AGATTCAGGG
  541 TATACATATA GGAAGAGCTG CATTTTGCCA TCAGGAGAAT GCAACCTGCC AGGACCTCAG
  601 TTTCTTCCTC TGCAAAATGC TCCCAAAGTA GATAGACCCT TCCACAACCT TCTGAGATTC
  661 TCTCAGCCTG ACTTGTGTGT TTATGTTGGA CCACAGTACT GTACTTGGTC CCATTAGGAA
  721 TTCTCATGTG AAGGATGATT CAGAAAAACC TTTGGTTAGG GCGCACATGG GTGTTCATGC
  781 CTTCCACAGG TTGGTTATGC AACCAAAACT TCAGAAAACT GAATATAAAA TGTGACCTTT
  841 TCATACCAAA CATAACCTCA GTCACGAACC AAAGCTTTGG CAATTATGTT ACATTGTCGG
  901 TCTGGTCCAG CTAACAGATT TTTAAAATGC ATTTCTGCAT GTCTATCCTT TAGTCCTACA
  961 CTCGATCTTC TCGGTTCCCT TGGGCTAGAT ATGCAGATCA AATATCCAGA TGAACATAGA
 1021 GAGTTTTACT GATAAGTAAA CATGCAACGC CCATACACAC CACATAATGT GCATTAATCT
 1081 AATAATATAT AATATTACGA TTAGGATAAA ATAGTGAAGT AATCATGCGC TCTATATCCT
1141 T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user report Chiu, J.H., Screening to identify commonly used chinese herbs that affect ERBB2 and ESR1 gene expression using the human breast cancer MCF-7 cell line. Evid. Based Complement. Alternat. Med., 965486 (2014). PMID 24987437.
user report Noomhorm, N., In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen. J. Pharmacol. Sci. 125 (4): 375-385 (2014). PMID 25141924.

2020.02.12

GNP_filter3_RDBDEP_html_191130.pl