RDB07528 - pGL4-phESR1

Resource data sheet
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pGL4-phESR1

Promoter collection, Human ESR1 promoter


Catalog number	RDB07528
Resource name	pGL4-phESR1
Alternative name	U2843-3
Clone info.	PCR amplified human ESR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 151806130 to 151807567(NC_000006.12); relatively -1549 to -111, where +1 corresponds to 1 nt of NM_000125.3; relatively -1189 to +249, where +1 corresponds to 1 nt of NM_001122740.1. PCR cloning, forward primer: TTGCTGGCCATAAAGGGAAA; reverse primer: CACTCCAGGCACAACTCGAT. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 genomic DNA
Depositor\|Developer	DNA Bank, \|
Other clones in our bank	human ESR1 (NCBI Gene 2099) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 　2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 　2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07528	pGL4-phESR1	DNA solution

Ordering Information [in Japanese] [in English]
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References and tips

Electronic file

Electronic file	Remarks, protocol and/or map (pdf) RDB07528.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content	Dnaconda's recommendation EXR0002j (Japanese text)
Featured content	Promoter Collection (Firefly Luciferase) (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12848_A4Im.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB12848_A4Ima.seq
>07528_12848_A4Im_pGL4-4174F_F07_16.ab1 1 AATGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TTTGCTGGCC ATAAAGGGAA ATATCTGCGA TGCACCTAAT GTGTTTTTAA CCCTTTATTT 121 GCTGACAATC TATAGTCATT AATGCTAAAC TCGATTTTGG CTTCAGCTAC ATTTGCATAT 181 TGTCCAACAA TGGTCTATTT TTGTAAGAAT TAGATAAAAT GTATACTTGA TATAAAATAG 241 TCAAAAATGT AACTCTTAGT AACAGTAAGC TTGGCATTTA GATAGACCAT GAACACTTCG 301 TCAGATACTC TGTTGGGTGT TTGGGATAGC AATTAAAACA AAGTATTGAT AGTTGTATCA 361 GAGTCTATTA GGCTGCAGCA AAGGAAGTTT ATTCAAAAGT ATAAACTATC CAAGATTATA 421 GACGCATGAT ATACTTCACC TATTTTTTGT CTCCTTAATA TGTATATATA TATATATATA 481 TATATATATA TACACATATA TGTGTGTGTG TATGTGCGTG TGCATGTTTA ACTTTTAATT 541 CAGTTAAAAA CTTTTTTCTA TTTGTTTTTC ATCTGGATAT TTGATTCTGC ATATCCTAGC 601 CCAAGTGAAC CGAGAAGATC GAGTTGTAGG ACTAAAGGAT AGACATGCAG AAATGCATTT 661 TAAAAATCTG TTAGCTGGAC CAGACCGACA ATGTAACATA ATTGCCAAAG CTTTGGTTCG 721 TGACCTGAGG TTATGTTTGG TATGAAAAGG TCACATTTTA TATTCAGTTT TCTGAAGTTT 781 TGGTTGCATA ACCAACCTGT GGAAGGCATG AACACCCATG TGCGCCCTAA CCAAAGGTTT 841 TTCTGAATCA TCCTTCACAT GAGAATTCCT AATGGGACCA AGTACAGTAC TGTGGTCCAA 901 CATAAACACA CAAGTCAGGC TGAGAGAATC TCAGAAGGTT GTGGAAGGGT CTATCTACTT 961 TGGGAGCATT TTGCAGAGGA AGAAACTGAG GTCCTGGCAG GTTGCATTCT CCTGATGGCA 1021 AAATGCAGCT CTTCCTATAT GTATACCCTG AATCTCCGCC CCCCTTTCCC CTCAGAATGC 1081 CCCCTGTCAG TTTCCCCCCA GCTGCTTAAA TATAGCCTGT CTGTTGCTGA CTTGCGTATG 1141 GCAACCGCAA CCAACCCCCA ATCCTATCCT GC //
Primer: pGL4-136R Sequence file: RDB12848_A4Imb.seq
>07528_12848_A4Im_pGL4-136R_G07_19.ab1 1 GCTTTCTTTG TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATCACTCC AGGCACAACT CGATTTGGAG CGATCCCAAA 121 GAGCAGCTTC CCTGAACTTT ACTTTACTTG TCGTCGCTGC TGGATAGAGG CTGAGTTTCA 181 CGGCCAGGGG GCGGGGGCGC ACGAGGATCT GCTAAAGGTG GCCCAGGGAA GACTGGGCTT 241 AAAATAAACG CGAAAGACGG ATCCAGGGGC CGGCTTTCTC TAATGTGCTG CCTTATGTGC 301 CCCGTGCCAG ACTCCAATAT ATCTCTGTTT GTCTCTCTTT CTGTTTGATT CCCCCTCCTC 361 GTTGGCTAGA AATACGTAGT GTGTACATAG GATGACCCTG GGGAGGACTA CACTGTAACC 421 GAGATAGGGC AGATAGAATG GGGTGTGCGG TTGCATACGC AGCCAGCCAC AGACAGCTAT 481 ATTTAGCAGC TGGGGGAACT GACAGGGGGC ATCTGAGGGG AAGGGGGCGG AGATTCAGGG 541 TATACATATA GGAAGAGCTG CATTTTGCCA TCAGGAGAAT GCAACCTGCC AGGACCTCAG 601 TTTCTTCCTC TGCAAAATGC TCCCAAAGTA GATAGACCCT TCCACAACCT TCTGAGATTC 661 TCTCAGCCTG ACTTGTGTGT TTATGTTGGA CCACAGTACT GTACTTGGTC CCATTAGGAA 721 TTCTCATGTG AAGGATGATT CAGAAAAACC TTTGGTTAGG GCGCACATGG GTGTTCATGC 781 CTTCCACAGG TTGGTTATGC AACCAAAACT TCAGAAAACT GAATATAAAA TGTGACCTTT 841 TCATACCAAA CATAACCTCA GTCACGAACC AAAGCTTTGG CAATTATGTT ACATTGTCGG 901 TCTGGTCCAG CTAACAGATT TTTAAAATGC ATTTCTGCAT GTCTATCCTT TAGTCCTACA 961 CTCGATCTTC TCGGTTCCCT TGGGCTAGAT ATGCAGATCA AATATCCAGA TGAACATAGA 1021 GAGTTTTACT GATAAGTAAA CATGCAACGC CCATACACAC CACATAATGT GCATTAATCT 1081 AATAATATAT AATATTACGA TTAGGATAAA ATAGTGAAGT AATCATGCGC TCTATATCCT 1141 T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user report	Chiu, J.H., Screening to identify commonly used chinese herbs that affect ERBB2 and ESR1 gene expression using the human breast cancer MCF-7 cell line. Evid. Based Complement. Alternat. Med., 965486 (2014). PMID 24987437.
user report	Noomhorm, N., In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen. J. Pharmacol. Sci. 125 (4): 375-385 (2014). PMID 25141924.

2020.10.02

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