RDB07528 - pGL4-phESR1

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pGL4-phESR1 (#RDB07528)

Promoter collection, Human ESR1 promoter

Alternative name	U2843-3
Clone info.	PCR amplified human ESR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 151806130 to 151807567(NC_000006.12); relatively -1549 to -111, where +1 corresponds to 1 nt of NM_000125.3; relatively -1189 to +249, where +1 corresponds to 1 nt of NM_001122740.1.
Comment	PCR cloning, forward primer: TTGCTGGCCATAAAGGGAAA; reverse primer: CACTCCAGGCACAACTCGAT. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07528.pdf

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07528	pGL4-phESR1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phESR1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07528).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12848_A4Im.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB12848_A4Ima.seq
>07528_12848_A4Im_pGL4-4174F_F07_16.ab1 1 AATGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TTTGCTGGCC ATAAAGGGAA ATATCTGCGA TGCACCTAAT GTGTTTTTAA CCCTTTATTT 121 GCTGACAATC TATAGTCATT AATGCTAAAC TCGATTTTGG CTTCAGCTAC ATTTGCATAT 181 TGTCCAACAA TGGTCTATTT TTGTAAGAAT TAGATAAAAT GTATACTTGA TATAAAATAG 241 TCAAAAATGT AACTCTTAGT AACAGTAAGC TTGGCATTTA GATAGACCAT GAACACTTCG 301 TCAGATACTC TGTTGGGTGT TTGGGATAGC AATTAAAACA AAGTATTGAT AGTTGTATCA 361 GAGTCTATTA GGCTGCAGCA AAGGAAGTTT ATTCAAAAGT ATAAACTATC CAAGATTATA 421 GACGCATGAT ATACTTCACC TATTTTTTGT CTCCTTAATA TGTATATATA TATATATATA 481 TATATATATA TACACATATA TGTGTGTGTG TATGTGCGTG TGCATGTTTA ACTTTTAATT 541 CAGTTAAAAA CTTTTTTCTA TTTGTTTTTC ATCTGGATAT TTGATTCTGC ATATCCTAGC 601 CCAAGTGAAC CGAGAAGATC GAGTTGTAGG ACTAAAGGAT AGACATGCAG AAATGCATTT 661 TAAAAATCTG TTAGCTGGAC CAGACCGACA ATGTAACATA ATTGCCAAAG CTTTGGTTCG 721 TGACCTGAGG TTATGTTTGG TATGAAAAGG TCACATTTTA TATTCAGTTT TCTGAAGTTT 781 TGGTTGCATA ACCAACCTGT GGAAGGCATG AACACCCATG TGCGCCCTAA CCAAAGGTTT 841 TTCTGAATCA TCCTTCACAT GAGAATTCCT AATGGGACCA AGTACAGTAC TGTGGTCCAA 901 CATAAACACA CAAGTCAGGC TGAGAGAATC TCAGAAGGTT GTGGAAGGGT CTATCTACTT 961 TGGGAGCATT TTGCAGAGGA AGAAACTGAG GTCCTGGCAG GTTGCATTCT CCTGATGGCA 1021 AAATGCAGCT CTTCCTATAT GTATACCCTG AATCTCCGCC CCCCTTTCCC CTCAGAATGC 1081 CCCCTGTCAG TTTCCCCCCA GCTGCTTAAA TATAGCCTGT CTGTTGCTGA CTTGCGTATG 1141 GCAACCGCAA CCAACCCCCA ATCCTATCCT GC //
Primer: pGL4-136R Sequence file: RDB12848_A4Imb.seq
>07528_12848_A4Im_pGL4-136R_G07_19.ab1 1 GCTTTCTTTG TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATCACTCC AGGCACAACT CGATTTGGAG CGATCCCAAA 121 GAGCAGCTTC CCTGAACTTT ACTTTACTTG TCGTCGCTGC TGGATAGAGG CTGAGTTTCA 181 CGGCCAGGGG GCGGGGGCGC ACGAGGATCT GCTAAAGGTG GCCCAGGGAA GACTGGGCTT 241 AAAATAAACG CGAAAGACGG ATCCAGGGGC CGGCTTTCTC TAATGTGCTG CCTTATGTGC 301 CCCGTGCCAG ACTCCAATAT ATCTCTGTTT GTCTCTCTTT CTGTTTGATT CCCCCTCCTC 361 GTTGGCTAGA AATACGTAGT GTGTACATAG GATGACCCTG GGGAGGACTA CACTGTAACC 421 GAGATAGGGC AGATAGAATG GGGTGTGCGG TTGCATACGC AGCCAGCCAC AGACAGCTAT 481 ATTTAGCAGC TGGGGGAACT GACAGGGGGC ATCTGAGGGG AAGGGGGCGG AGATTCAGGG 541 TATACATATA GGAAGAGCTG CATTTTGCCA TCAGGAGAAT GCAACCTGCC AGGACCTCAG 601 TTTCTTCCTC TGCAAAATGC TCCCAAAGTA GATAGACCCT TCCACAACCT TCTGAGATTC 661 TCTCAGCCTG ACTTGTGTGT TTATGTTGGA CCACAGTACT GTACTTGGTC CCATTAGGAA 721 TTCTCATGTG AAGGATGATT CAGAAAAACC TTTGGTTAGG GCGCACATGG GTGTTCATGC 781 CTTCCACAGG TTGGTTATGC AACCAAAACT TCAGAAAACT GAATATAAAA TGTGACCTTT 841 TCATACCAAA CATAACCTCA GTCACGAACC AAAGCTTTGG CAATTATGTT ACATTGTCGG 901 TCTGGTCCAG CTAACAGATT TTTAAAATGC ATTTCTGCAT GTCTATCCTT TAGTCCTACA 961 CTCGATCTTC TCGGTTCCCT TGGGCTAGAT ATGCAGATCA AATATCCAGA TGAACATAGA 1021 GAGTTTTACT GATAAGTAAA CATGCAACGC CCATACACAC CACATAATGT GCATTAATCT 1081 AATAATATAT AATATTACGA TTAGGATAAA ATAGTGAAGT AATCATGCGC TCTATATCCT 1141 T //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report	Chiu, J.H., Screening to identify commonly used chinese herbs that affect ERBB2 and ESR1 gene expression using the human breast cancer MCF-7 cell line. Evid. Based Complement. Alternat. Med., 965486 (2014). PMID 24987437. [link to RRC of NBRP]
user_report	Noomhorm, N., In vitro and in vivo effects of xanthorrhizol on human breast cancer MCF-7 cells treated with tamoxifen. J. Pharmacol. Sci. 125 (4): 375-385 (2014). PMID 25141924. [link to RRC of NBRP]

Resource search

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Other resources in our bank

human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1

External Database
Human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1

Reference sequence