Please review the QC test results indicated by
icon as well as clone information before placing your order. icon below as well as clone information before placing your order.
Promoter collection, Human ESR1 promoter
| Catalog number | RDB07528 |
|---|---|
| Resource name | pGL4-phESR1 |
| Alternative name | U2843-3 |
| Clone info. | PCR amplified human ESR1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 151806130 to 151807567(NC_000006.12); relatively -1549 to -111, where +1 corresponds to 1 nt of NM_000125.3; relatively -1189 to +249, where +1 corresponds to 1 nt of NM_001122740.1. PCR cloning, forward primer: TTGCTGGCCATAAAGGGAAA; reverse primer: CACTCCAGGCACAACTCGAT. Entire sequence of promoter region has not been confirmed. |
| Vector backbone | pGL4.10 [Luc2] (Plasmid) |
| Selectable markers | Amp^r |
| Gene/insert name | Human RP1-130E4.1, DKFZp686N23123, ER, ESR, ESRA, Era, NR3A1 genomic DNA |
| Depositor|Developer | DNA Bank, | |
| Other clones in our bank | human ESR1 (NCBI Gene 2099) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
|---|---|
| Additional terms and conditions for distribution | The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB07528 | pGL4-phESR1 | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Electronic file | Remarks, protocol and/or map (pdf) RDB07528.pdf |
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Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Dnaconda's recommendation EXR0002j (Japanese text) |
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| Featured content | Promoter Collection (Firefly Luciferase) (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB12848_A4Im.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: pGL4-4174F Sequence file: RDB12848_A4Ima.seq
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>07528_12848_A4Im_pGL4-4174F_F07_16.ab1 1 AATGGGTCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TTTGCTGGCC ATAAAGGGAA ATATCTGCGA TGCACCTAAT GTGTTTTTAA CCCTTTATTT 121 GCTGACAATC TATAGTCATT AATGCTAAAC TCGATTTTGG CTTCAGCTAC ATTTGCATAT 181 TGTCCAACAA TGGTCTATTT TTGTAAGAAT TAGATAAAAT GTATACTTGA TATAAAATAG 241 TCAAAAATGT AACTCTTAGT AACAGTAAGC TTGGCATTTA GATAGACCAT GAACACTTCG 301 TCAGATACTC TGTTGGGTGT TTGGGATAGC AATTAAAACA AAGTATTGAT AGTTGTATCA 361 GAGTCTATTA GGCTGCAGCA AAGGAAGTTT ATTCAAAAGT ATAAACTATC CAAGATTATA 421 GACGCATGAT ATACTTCACC TATTTTTTGT CTCCTTAATA TGTATATATA TATATATATA 481 TATATATATA TACACATATA TGTGTGTGTG TATGTGCGTG TGCATGTTTA ACTTTTAATT 541 CAGTTAAAAA CTTTTTTCTA TTTGTTTTTC ATCTGGATAT TTGATTCTGC ATATCCTAGC 601 CCAAGTGAAC CGAGAAGATC GAGTTGTAGG ACTAAAGGAT AGACATGCAG AAATGCATTT 661 TAAAAATCTG TTAGCTGGAC CAGACCGACA ATGTAACATA ATTGCCAAAG CTTTGGTTCG 721 TGACCTGAGG TTATGTTTGG TATGAAAAGG TCACATTTTA TATTCAGTTT TCTGAAGTTT 781 TGGTTGCATA ACCAACCTGT GGAAGGCATG AACACCCATG TGCGCCCTAA CCAAAGGTTT 841 TTCTGAATCA TCCTTCACAT GAGAATTCCT AATGGGACCA AGTACAGTAC TGTGGTCCAA 901 CATAAACACA CAAGTCAGGC TGAGAGAATC TCAGAAGGTT GTGGAAGGGT CTATCTACTT 961 TGGGAGCATT TTGCAGAGGA AGAAACTGAG GTCCTGGCAG GTTGCATTCT CCTGATGGCA 1021 AAATGCAGCT CTTCCTATAT GTATACCCTG AATCTCCGCC CCCCTTTCCC CTCAGAATGC 1081 CCCCTGTCAG TTTCCCCCCA GCTGCTTAAA TATAGCCTGT CTGTTGCTGA CTTGCGTATG 1141 GCAACCGCAA CCAACCCCCA ATCCTATCCT GC // |
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Primer: pGL4-136R Sequence file: RDB12848_A4Imb.seq
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>07528_12848_A4Im_pGL4-136R_G07_19.ab1 1 GCTTTCTTTG TTTTTGGCAT CTTCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATCACTCC AGGCACAACT CGATTTGGAG CGATCCCAAA 121 GAGCAGCTTC CCTGAACTTT ACTTTACTTG TCGTCGCTGC TGGATAGAGG CTGAGTTTCA 181 CGGCCAGGGG GCGGGGGCGC ACGAGGATCT GCTAAAGGTG GCCCAGGGAA GACTGGGCTT 241 AAAATAAACG CGAAAGACGG ATCCAGGGGC CGGCTTTCTC TAATGTGCTG CCTTATGTGC 301 CCCGTGCCAG ACTCCAATAT ATCTCTGTTT GTCTCTCTTT CTGTTTGATT CCCCCTCCTC 361 GTTGGCTAGA AATACGTAGT GTGTACATAG GATGACCCTG GGGAGGACTA CACTGTAACC 421 GAGATAGGGC AGATAGAATG GGGTGTGCGG TTGCATACGC AGCCAGCCAC AGACAGCTAT 481 ATTTAGCAGC TGGGGGAACT GACAGGGGGC ATCTGAGGGG AAGGGGGCGG AGATTCAGGG 541 TATACATATA GGAAGAGCTG CATTTTGCCA TCAGGAGAAT GCAACCTGCC AGGACCTCAG 601 TTTCTTCCTC TGCAAAATGC TCCCAAAGTA GATAGACCCT TCCACAACCT TCTGAGATTC 661 TCTCAGCCTG ACTTGTGTGT TTATGTTGGA CCACAGTACT GTACTTGGTC CCATTAGGAA 721 TTCTCATGTG AAGGATGATT CAGAAAAACC TTTGGTTAGG GCGCACATGG GTGTTCATGC 781 CTTCCACAGG TTGGTTATGC AACCAAAACT TCAGAAAACT GAATATAAAA TGTGACCTTT 841 TCATACCAAA CATAACCTCA GTCACGAACC AAAGCTTTGG CAATTATGTT ACATTGTCGG 901 TCTGGTCCAG CTAACAGATT TTTAAAATGC ATTTCTGCAT GTCTATCCTT TAGTCCTACA 961 CTCGATCTTC TCGGTTCCCT TGGGCTAGAT ATGCAGATCA AATATCCAGA TGAACATAGA 1021 GAGTTTTACT GATAAGTAAA CATGCAACGC CCATACACAC CACATAATGT GCATTAATCT 1081 AATAATATAT AATATTACGA TTAGGATAAA ATAGTGAAGT AATCATGCGC TCTATATCCT 1141 T // |
Please visit 
Sequencing and PCR primers for primer information.
Original, user report and related articles
2020.02.12
GNP_filter3_RDBDEP_html_191130.pl