RDB07516 - pGL4-phTIMP1

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pGL4-phTIMP1 (#RDB07516)

Promoter collection, Human TIMP1 promoter

Alternative name	U2805-2
Clone info.	PCR amplified human TIMP1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 47581006 to 47582331(NC_000023.11); relatively -1430 to -104, where +1 corresponds to 1 nt of NM_003254.3.
Comment	PCR cloning, forward primer: GGGCTTTTAGTGTCCCCATC; reverse primer: ACCCACCATCAGTGCAGAA. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human TIMP metallopeptidase inhibitor 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07516.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07516	pGL4-phTIMP1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phTIMP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07516).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07516_A8Abp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07516_A8Aba.seq
>07516_07516_A8Ab_pGL4-4174F_F01_06_ABI08.ab1 1 GCTGGGTTTC AGGACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA 61 GGATGGGCTT TTAGTGTCCC CATCAACCAA ATAGTGTACA TTGTACCCAT TAGGCAATTT 121 CTCATCCCTC ACATTCCTCC CACCCAGAAT AGTGCTGGAT ACGCAGTAAG CACTCCATAA 181 GGGCTGCTGG TAGTTTTTGT TGTGTTCTTG TTATTGGGCT ATCATCCCTG AGTTAATCAA 241 ACTTAATTAG CAAGGGAGTA TGGTGAGTGA ATGAGATAAT TTATACAAAC ACTTTAGCAC 301 ATAGTACACA GAAAGTGCTC AATAAGTGGC TGCCATCATC ACTACAATTA TTATTATTGA 361 TCTATCCTCC TCGAATTAAG GATGTTAATC AGAACCCCAG GGAAGGTTCC TGCATTGTCA 421 GAAAGCTGGT GGGCAAGGAT TGCCCCACCC CACCCCCCAC CAGGGGTACA GGTCCATGGG 481 GAGGGGGCAG GGCAGGATGG GGTGGGGATT AGTTTTCTAC TGACCCACTC ACTTGCTTCT 541 TTCACCATCA TTCATGGCCA CCCCCTTCCC AATGCTCATT CATTCACCCG CACCATTCCC 601 TGCTTGCTAG GACGGTGCCA CTCCTGAGTT ACAACCCTGG TGCCAGGTAG CCTCTGTTTG 661 AATCCCGGCC ATGCCCTGTG ATCTTAGGAA AATTCTACAG TTCCTCTACG CCCCATATTT 721 CCTCATCCGT AAAACGGGAA TAAGAACCGG TACCCATCTC AGAGATTTGT TGTGAGCTTT 781 GAGTGAGATA AACTATGCTG AGTGCCTGGT ATACAGTAGG TGCTGTATAA ATGCCGGCTA 841 TTTGCCTGTG TTATTTGAGA CCCTGGCTTT GGCTCCTGGC CACCTGAGTT CCAGTCTCAG 901 TTCTGCCATG TATTGACTCT GTGATCCTGG GTAAGTCACT TAACCACTCC GTGCCTCAGT 961 TTCCCCATTT GTATTCCTCC CCTTTCACCT GCCTTATCTC CTCCACTGCT GCTACTTAAT 1021 TTGTTTCCTC TCTGCCACCC CTCACCAGCA TGTCAAACAT ACAAAAACAA GGGATTTTTG 1081 TGTGCTTTGG CACACCAGTA GAATGCACAA TTAATTGTTT GAAAGGGGCT TGAAACTAAT 1141 TTTGGGGTTT GAGAGTCATA TAAGGGGAGA GCTTCTTGGG TG //
Primer: pGL4-136R Sequence file: RDB07516_A8Abb.seq
>07516_07516_A8Ab_pGL4-136R_G01_07_ABI08.ab1 1 CCCCCATAGG GGTTTTTTGG CATCTTCCAT GGTGGCTTTA CCAACAGTAC CGGATTGCCA 61 AGCTTGGCCG CCGAGGCCAG ATCTTGATAC CCACCATCAG TGCAGAAGCC AAGGGGCGGG 121 CCGACGAAAG GAGATACACC CTCCTCCTCT CCCACCCGCC TCCTCTCCCT CTCCCTGGGC 181 CTCGCCCGGG CCAGCCACCT CCCCCACCTC CACGTCAGTC ACTATTCCTT CCAAGCCGCC 241 TCAGCGGAGA GCTTAGAGGC CGCATTTCCC CCGGCCCAGT CCCCTAAAGT CTCCAGAATC 301 TATCCAATCA CCCACATCCC CCAAGCTCCC TATGACTCAA ACCCAAATTA GTTCAGCCCT 361 TCAACATTTA TTGTGCATCT ACTGTGTGCC AAGCACACAA AAATCCCTTG TTTTGTATGT 421 CTGACATGCT GGTGAGGGGT GGCAGAGAGG AAACAAATTA AGTAGCAGCA GTGGAGGGAG 481 ATAAGGCAGG TGAAAGGGGA GGAATACAAA ATGGGGAAAC TGAGGCACGG AGTGGTTAAG 541 TGACTTACCC AGGATCACAG AGTCAATACA TGGCAGAACT GAGACTGGAA CTCAGGTGGC 601 CAGGAGCCAA AGCCAGGGTC TCAAATAACA CAGGCAAATA GCCGGCATTT ATACAGCACC 661 TACTGTATAC CAGGCACTCA GCATAGTTTA TCTCACTCAA AGCTCACAAC AAATCTCTGA 721 GATGGGTACC GGTTCTTATT CCCGTTTTAC GGATGAGGAA ATATGGGGCG TAGAGGAACT 781 GTAGAATTTT CCTAAGATCA CAGGGCATGG CCGGGATTCA AACAGAGGCT ACCTGGCACC 841 AGGGTTGTAA CTCAGGAGTG GCACCGTCCT AGCAAGCAGG GAATGGTGCG GGTGAATGAA 901 TGAGCATTGG GAAGGGGGTG GCCATGAATG ATGGTGAAAG AAGCAAGTGA GTGGGTCAGT 961 AGAAAACTAA TCCCCACCCC ATCCTGCCCT GCCCCCTCCC CATGGACCTG TACCCCTGGT 1021 GGGGGGGGTT GGGGGTGGGG //
Primer: pAxCALNL_F1 Sequence file: RDB07516_A8Abc.seq
>07516_07516_A8Ab_pAxCALNL_F1_H01_08_ABI08.ab1 1 CTCCAGTGGT AATCTTTATT ATGTCTGCTC GAAGCGGCCG GCCGCCCCGA CTCTAGAATT 61 ATTACACGGC GATCTTGCCG CCCTTCTTGG CCTTAATGAG AATCTCGCGG ATCTTGCGGG 121 CGTCCAACTT GCCGGTCAGT CCTTTAGGCA CCTCGTCCAC GAACACAACA CCACCGCGCA 181 GCTTCTTGGC GGTTGTAACC TGGCTGGCCA CATAGTCCAC GATCTCCTTC TCGGTCATGG 241 TTTTACCGTG TTCCAGCACG ACGACTGCGG CGGGCAGCTC GCCGGCATCG TCGTCGGGCA 301 GGCCGGCGAC CCCGGCGTCG AAGATGTTGG GGTGTTGCAG CAGGATGCTC TCCAGTTCGG 361 CTGGGGCTAC CTGGTAGCCC TTGTATTTGA TCAGGCTCTT CAGCCGGTCC ACGATGAAGA 421 AGTGCTCGTC CTCGTCCCAG TAGGCGATGT CGCCGCTGTG CAGCCAGCCG TCCTTGTCGA 481 TGAGAGCGTT TGTAGCCTCG GGGTTGTTAA CGTAGCCGCT CATGATCATG GGGCCACGGA 541 CGCACAGCTC GCCGCGCTGG TTCACACCCA GTGTCTTACC GGTGTCCAAG TCCACCACCT 601 TAGCCTCGAA GAAGGGCACC ACCTTGCCTA CTGCGCCAGG CTTGTCGTCC CCTTCGGGGG 661 TGATCAGAAT GGCGCTGGTT GTTTCTGTCA GGCCGTAGCC CTGGCGGATG CCTGGTAGGT 721 GGAAGCGTTT GGCCACGGCC TCACCTACCT CCTTGCTGAG CGGCGCCCCG CCGCTGGCGA 781 TCTCGTGCAA GTTGCTTAGG TCGTACTTGT CGATGAGAGT GCTCTTAGCG AAGAAGCTAA 841 ATAGTGTGGG CACCAGCAGG GCAGATTGAA TCTTATAGTC TTGCAAGCTG CGCAAGAATA 901 GCTCCTCCTC GAAGCGGTAC ATGAGCACGA CCCGAAAGCC GCAGATCAAG TAGCCTCAGC 961 GTGGTGAACA TGCCGAAGCC GTGGTGAAAT GGCACCACGC TGAGGATAGC GGTGTCGGGG 1021 ATGATCTGGT TTGCCGAAGA TGGGGTCGCG GCCATGACTG AATCGGACAC AAGCGGTGCG 1081 TGCGGTAGGG CCTACGCCCC TTGGGCATTC GGGTACTGCC ACTACTATTC AATGGAATCA 1141 GGCCGAATGG TTTTGATCCC GGTTCGAAGC CCTCCTCGGG CCACGAAGGT CGTATCTCTT 1201 CCGTTTGAGA AAGACTG //