RDB07501 - pCMFlag_hsCREB1, transcript variant A

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCMFlag_hsCREB1, transcript variant A (#RDB07501)

Expression vector of human CREB1.

Alternative name	SET-0020_8-1
Clone info.	Expression vector of human CREB1. Expression has not yet been confirmed.
Comment	PCR cloning, forward primer: ATGACCATGGAATCTGGAGCCGA; reverse primer: AATTAATCTGATTTGTGGCAGTAAA.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human CREB1,transcript variant A cDNA
Depositor\|Developer	DNA Bank, \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
Additional terms and conditions:
Regrding pRc/CMV:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	発表に際し、理研BRCから入手したことを明記すること。付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

発表に際し、理研BRCから入手したことを明記すること。
付加的使用条件：
pRc/CMVについて:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

Catalog #	Resource name	Shipping form	Fee
RDB07501	pCMFlag_hsCREB1, transcript variant A	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsCREB1, transcript variant A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07501).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07501_A1L1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,FLAG,insert 5' Sequence file: RDB07501_A1L1a.seq
>07501_07501_B1AjF_CMV-Forward_E10_13_ABI24.ab1 1 NNNNNNNNNN NNNNNTNGNN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG 181 GCGGCCATGA CCATGGAATC TGGAGCCGAG AACCAGCAGA GTGGAGATGC AGCTGTAACA 241 GAAGCTGAAA ACCAACAAAT GACAGTTCAA GCCCAGCCAC AGATTGCCAC ATTAGCCCAG 301 GTATCTATGC CAGCAGCTCA TGCAACATCA TCTGCTCCCA CCGTAACTCT AGTACAGCTG 361 CCCAATGGGC AGACAGTTCA AGTCCATGGA GTCATTCAGG CGGCCCAGCC ATCAGTTATT 421 CAGTCTCCAC AAGTCCAAAC AGTTCAGATT TCAACTATTG CAGAAAGTGA AGATTCACAG 481 GAGTCAGTGG ATAGTGTAAC TGATTCCCAA AAGCGAAGGG AAATTCTTTC AAGGAGGCCT 541 TCCTACAGGA AAATTTTGAA TGACTTATCT TCTGATGCAC CAGGAGTGCC AAGGNNTGAA 601 GAAGAGAAGT CTGAANAGGA NANTTCNGCA CCTGCCNTCA CCACTGNNAC GGTGCNNACT 661 CCNATTTACC NAACTNGCAG TGGNNAGNAT ATNGNNNTNA CCNNNGGNNN ANNNNNNNNC 721 NNGNNNNNNN NNN //
Primer: bGH_rev3 (Pr0885) Region: bGHpA,insert 3' Sequence file: RDB07501_A1L1b.seq
>07501_07501_B1AjF_bGH_rev3_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNG NNGGGCANAC AANNGATGGC TGGCAACTAG AAGGCACAGT 61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATG 121 CATGCTCGAG CGGCCAATTA ATCTGATTTG TGGCAGTAAA GGTCCTTAAG TGCTTTTAGC 181 TCCTCAATCA ATGTCTTGTT TTGATTTTCA AGCACTGCCA CTCTGTTTTC TAAACATTTC 241 ACATATTCTT TCTTCTTTCT ACGACACTCT CGAGCTGCTT CCCTGTTCTT CATTAGACGG 301 ACCTCTCTCT TTCGTGCTGC TTCTTCAGCA GGCTGTGTAG GAAGTGCTGG GGAGGATGCC 361 ATAACAACTC CAGGGGCAAT AGTGCTAGTG GGTGCTGTGC GAATCTGGTA TGTTTGTACG 421 TCTCCAGAGG CAGCTTGAAC AACAACTTGG TTGCTGGGCA CTAAGATCTG CTGTCCATCA 481 GTGGTCTGTG CATACTGTAG AATGGTAGTA CCCGGCTGAG TGGCTGCTGC ATTGGTCATG 541 GTTAATGTTT GCAGGCCCTG TACCCCATCG GTACCATTGT TAGCCAGCTG TATTGCTCCT 601 CCCTGGGTAA TGGCAATATA CTGTCCACTG CTAGTTTGGT AAATTGGAGT TGGCANCGTT 661 ACAGTGGTGA NGGCANGNGC TGNANTCTCC TCNTCNNANT NCTCNNCNNC NNNNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB07501_A1L1c.seq
>07501_07501_B1AjF_SV40pro_ori_F_E12_15_ABI24.ab1 1 NNNNNNNNNC NNNGCCGCCT CGGCCTCTGA NCTATTCCNG AAGTAGTGAG GAGGCTTTTT 61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGG ATATCCATTT TCGGATCTGA 121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC 181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG 241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC 301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC 361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG 421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA 481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG 541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG 601 TCTTGTCNAT CANGANGANC TGGANNAANA NCATCNNGGG CTCGCGNCNG CCNAACTGNT 661 CGCCNGGCTC NANGNNCNNA NGNCCNANNN NAGNNNCNCN NCNNGNNCNN NNNNNANGNN 721 NGNNNGNNNA ANANNNNNNN NNN //