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pGL4-phPLAU

Promoter collection, Human PLAU promoter

Catalog number RDB07487
Resource name pGL4-phPLAU
Alternative name U2833-5
Clone info. PCR amplified human PLAU promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 73909783 to 73911185(NC_000010.11); relatively -1318 to +85, where +1 corresponds to 1 nt of NM_002658.4.
Comment PCR cloning, forward primer: GGGTTCAAAATGACCCCAAG; reverse primer: TGCGGGGACAGGTGGACCCT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human plasminogen activator, urokinase genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human plasminogen activator, urokinase

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07487 pGL4-phPLAU DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07487.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0096e (English text)
          Featured content Dnaconda's recommendation EXR0096j (Japanese text)
          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07487_A6Gep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07487_A6Gea.seq check
          >07487_07487_A6Ge_pGL4-4174F_F01_16.ab1
              1 AAGGGTCAGA CTTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCGCTAGC CTCGAGGATG
             61 GGTTCAAAAT GACCCCAAGC CCTGGTTCCT GACACTAGGG GAAAGAGATG GGGGTGACAG
            121 AATCACAGAA TCCCTGCTAT GTTCCTCCAA GTGTGCCCAG AGATGCGTGT GTGTGTGTGT
            181 GTATACACAA ATGTCTGCTT ATCCTCAGGC AGGAAGGGTG GATGCAGTCA TTTACACATG
            241 GTCTGTTTTT CTGGAGGACA ATTTTATTTG ATAAACAATT GTTTCTATCT GAATAGAATA
            301 AACAAGGCTC TATGATGAAG TAAAACACTA AATACACATG CATTAAAAAA TGCATAATTA
            361 TCTTTTTGGA ATGGGCTATA CAGAGATGTG CTTTTTAAAA TGTTAAGAGT GTAAAAGGAC
            421 AAACAGTGAA AAATAAATCT TCCTCTTATT TTGTCCTCCA GTCTCCCAAT TCCTCTACTC
            481 AGAGGTGAGA ACAGAACTTC CACACCCTCC AGAACCTCCA CAGTTAGAAC TGTCTACATG
            541 TTTCCATTGT CTTTACTTTT ATTCTTGCCT GCACAAATAA ATGAATTGCT CATTATGGAA
            601 ACTTCCCAAA AGACCCGTTA ACACTTCAAT AGGAAGCACC AACAGTTTAT GCCCTAGGAC
            661 TTTGTTCCCA CAATCCTGTA ACATCATATC ACGACACCTA ACCCAATCCT TATCAAGCCC
            721 TGTCAAAAAC GGACTTTAAA CCAAGCTGCA AATTTTCAGT AATCTGGCCT TGCCTTTCCC
            781 CCTCTGATAG CACCATCAAA CAAACCCCCT TACTGCCGAA AGCAATAAGC CCGGCTTTGT
            841 TCCATCCACT GGTTGTGTTG GTGATATCTG GGGACTGCCA CTGAACAGAC GCACAGAGGG
            901 AGCCCCTACA GGCACGGGTT TTTCTGTCTG TGCTTCTGGG AGAGTATGTC TCGTACATTT
            961 TGTCGCGTTG ATGAAAGACT TCACAGCTCC ATCAGCTGCG GGCAAAGGGG TCTGGAGCAG
           1021 TCTTTCGCAG TTGGGGGCCC AGCGGGGGAA GAAGCTGCCA GAGCACCTGA ATTAGAGGAA
          1081 CCCCCAGGGC AAGGGT
          //
          Primer: pGL4-136R
          Sequence file: RDB07487_A6Geb.seq check
          >07487_07487_A6Ge_pGL4-136R_G01_19.ab1
              1 GCTTAGTTTT GGCATCTTCA TGGTGGCTTT ACCAACAGTA CCGGATTGCC AAGCTTGGCC
             61 GCCGAGGCCA GATCTTGATT GCGGGGACAG GTGGACCCTG GCCCGGGCTC CGGGGCTGCG
            121 GTCTCCGCAC TGTGCTGCGA CCCGCGGCGC CTGCTCTATA TCAGGGCCCG CCCCGGCGCC
            181 GCCCCTCCCT CTCCCGCCCG GCTCCCTCCC CTGTCTTGCA GCGCTCAGCG ACCCGGACCC
            241 CGCGTGCTTC CGCAACGCTC ACAAAGATTT GGGGGAAGCG CGATCTCCAG CGGAGGGGAC
            301 CCAACAGCGT CTGGACTGAG GAATCGAGAG GCTTGTAAAT TCTCCGTGCT TCCTCCCATG
            361 CACCTGGCCG GGGGCCTGCC CCAGTGCAAG GAGTCCCCGA ATTGCAGAGA GGAGAGAAGG
            421 CGCACAGGAG ACTCTCTACC TCGCCCAGCT CTGAAGCCTC CTGGGGTCCT CTAATCAGTT
            481 CTTCTGCAAC TTCTCCCCGC TGGGCCCCAA CTTGCCTAAG ACTGCCTCAG ACCCCCTTGC
            541 CCGCAGCTGA TGGAGCTGTG AAGTCTTCAT CAACGCGACA AATGTACGAG ACATACTCTC
            601 CCAGAAGCAC AGACAGAAAA ACCCCTGCCT GTAGGGGCTC CCTCTGTGCG TCTGTTCAGT
            661 GGCAGTCCCC AGATATCACC AACACAACCA GTGGATGGAA CAAAGCCGGG CTTATTGCTT
            721 TCGGCAGTAA GGGGGTTTGT TTGATGGTGC TATCAGAGGG GGAAAGGCAA GGCCAGATTA
            781 CTGAAAATTT GCAGCTTGGT TTAAAGTCCG TTTTTGACAG GGCTTGATAA GGATTGGGTT
            841 AGGTGTCGTG ATATGATGTT ACAGGATTGT GGGAACAAAG TCCTAGGGCA TAAACTGTTG
            901 GTGCTTCCTA TTGAAGTGTT AACGGGTCTT TTGGGAAAGT TTCCATAATG AGCAAATTCA
            961 TTTATTTGTG CAGGCAAGAA TAAAAGTAAA GACATGGGAA ACATGGTAGA ACAGCTCTTA
           1021 CTGTTGGAGG ATCCTGAAGG GATGTGAAAG GTCTGATCTC ACATCTGAGT AGAGAACTTG
          1081 GGGGAGAACT GG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07487_A6Gec.seq check
          >07487_07487_A6Ge_pAxCALNL_F1_H01_22.ab1
              1 TATTATCTTA TCATGTCTGC TCGAGCGGCC GGCCGCCCCG ACTCTAGAAT TATTACACGG
             61 CGATCTTGCC GCCCTTCTTG GCCTTAATGA GAATCTCGCG GATCTTGCGG GCGTCCAACT
            121 TGCCGGTCAG TCCTTTAGGC ACCTCGTCCA CGAACACAAC ACCACCGCGC AGCTTCTTGG
            181 CGGTTGTAAC CTGGCTGGCC ACATAGTCCA CGATCTCCTT CTCGGTCATG GTTTTACCGT
            241 GTTCCAGCAC GACGACTGCG GCGGGCAGCT CGCCGGCATC GTCGTCGGGC AGGCCGGCGA
            301 CCCCGGCGTC GAAGATGTTG GGGTGTTGCA GCAGGATGCT CTCCAGTTCG GCTGGGGCTA
            361 CCTGGTAGCC CTTGTATTTG ATCAGGCTCT TCAGCCGGTC CACGATGAAG AAGTGCTCGT
            421 CCTCGTCCCA GTAGGCGATG TCGCCGCTGT GCAGCCAGCC GTCCTTGTCG ATGAGAGCGT
            481 TTGTAGCCTC GGGGTTGTTA ACGTAGCCGC TCATGATCAT GGGGCCACGG ACGCACAGCT
            541 CGCCGCGCTG GTTCACACCC AGTGTCTTAC CGGTGTCCAA GTCCACCACC TTAGCCTCGA
            601 AGAAGGGCAC CACCTTGCCT ACTGCGCCAG GCTTGTCGTC CCCTTCGGGG GTGATCAGAA
            661 TGGCGCTGGT TGTTTCTGTC AGGCCGTAGC CCTGGCGGAT GCCTGGTAGG TGGAAGCGTT
            721 TGGCCACGGC CTCACCTACC TCCTTGCTGA GCGGCGCCCC GCCGCTGGCG ATCTCGTGCA
            781 AGTTGCTTAG GTCGTACTTG TCGATGAGAG TGCTCTTAGC GAAGAAGCTA AATAGTGTGG
            841 GCACCAGCAG GGCAGATTGA ATCTTATAGT CTTGCAAGCT GCGCAAGAAT AGCTCCTCCT
            901 CGAAGCGGTA CATGAGCACG ACCCGAAAGC CGCAGATCAA GTAGCCCAGC GTGGGTGAAA
            961 CATGCCGAAG CCGTGGTGAA ATGGGCACCA CGCTGAGGAT AGCGGTGTCG GGGATGATCT
           1021 GGTTGCCGGA GATGGGGTCG CGGGGCATGA CTGCATCCGG ACAACAAAGG CCGGATGGCC
          1081 GGATTGAGCG GATAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Resmini, G., HMGA1 regulates the Plasminogen activation system in the secretome of breast cancer cells. Sci. Rep. 7 (1): 11768 (2017). PMID 28924209.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl