RDB07482 - pGL4-phCSF2

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pGL4-phCSF2 (#RDB07482)

Promoter collection, Human CSF2 promoter

Alternative name	U2815-1
Clone info.	PCR amplified human CSF2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 132072406 to 132073823(NC_000005.10); relatively -1383 to +35, where +1 corresponds to 1 nt of NM_000758.4.
Comment	PCR cloning, forward primer: CTCCACCTGCTCTCTGAAGG; reverse primer: CCTCCAGAGAACTTTAGCCTTTCT. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human colony stimulating factor 2 (granulocyte-macrophage) genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07482.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07482	pGL4-phCSF2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCSF2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07482).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07482_A3Hr.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB07482_A3Hra.seq
>07482_07482_A3Hr_pGL4-4174F_G01_19.ab1 1 GCCGGGGTCA GAACATTTCT CTGGCCTACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG 61 ATCTCCACCT GCTCTCTGAA GGCTCGCCAA CCTGAGTCCA GCAGAATGTT CTCGCTTGTG 121 TCCAACCCCA CTGGTTTAGG CTGAATCAGC CTCTAGGGCC CAGAGGCACT GCACCTGGAG 181 TAGGGAGCTT CTCCAGTATC AGAGTCACCT TCAGAGGCCT GGAGCCTTTC ATAAAGCAGG 241 TAAGAGGACT CAATAGATGC ATCTGCATGG AAAACATCCT CCCCTCTACC AGGCACCTGT 301 ATGTACAACC AATCACAGCA GCACACATAC ACCCAGAAAT GGGCACGTGT GGGCCCACAC 361 CCCTTTAGCT ATGAAACCCA GGCATGGGGC AGCTTGAGCC AGATACCTTG TGCAAACACA 421 AACTCGTGCT GTCTTCTCTG AACTCCATTG TGAAAATCAA ACACTTGTCA GCCCCTCAAG 481 AGCCTTTAGA TTTCCTACTT CCACACTTCC ACAGAAAGGC CTCTGGAGTT GGGGGATGCT 541 GGGGTTATGT AGGAAATTAA GCCTGGAGGG CCTTGCTGGG GAAGCCATTG TCCCTGTACC 601 TGAGATGGAT GCAGCCACAG CCCTGGAGCC AGCCTGAAGC TCCTGGTGTC TTCTGGGGGC 661 TACATATAGG AG //
Primer: Sequence file: RDB07482_A3Hrb.seq
>07482_07482_A3Hr_pGL4-136R_H01_22.ab1 1 GGTCTAATGT TTTTGGCATC TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT 61 TGGCCGCCGA GGCCAGATCT TGATCCTCCA GAGAACTTTA GCCTTTCTCT CTGTGTACTG 121 GGCTCACTGG CAAAAGAGCT CTTAAATACA CAGAGGAAAT GATTAATGGT GACCACAAAA 181 TGCCAGGGAG GCGGGGGAAC TACCTGAACT GTGGAATCTC CTGGCCCTTA TCAGCCACAC 241 ATGGGAACGG TGAGCCTTTT CCCTAGGTGG TCAGGCTTGG GGGTTTTCAT TAATGAACCT 301 TTCCAAGAAC CGACAGCCCA CCCACCCGCC TTCCTGAGGG CTCTCCAGCC CTCCCTGGGC 361 AGTCTGAATG GGCCTGAGGC TGCCCCCTCC CTCTGAGGGG CACAGTTTGG ACTTCCTGGC 421 CTGGAATGGC TGGGGTGGGG CGTGGGAGAC ACTTAGATAG GGCTCCCCAT CCTGCCTGTA 481 ATCCCAGGGG CCTTTGGGCA GGCTATGCCC GCCCTGGTGC CTCATTCTGA CTCCAGCCTT 541 CCTCTTCTCT GGCCACTGTG AGAGACTTGA GTGTGAGGGG AGCTCTCACA GACCTGCCCC 601 ACTGACAGTT CACATGGGCT CCCACCCAGG ACCTGGAGCA GGGGGCAACC TCAGTCCAGT 661 AAGGGGGGAC CCCTGCC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

human colony stimulating factor 2 (granulocyte-macrophage)

External Database
Human colony stimulating factor 2 (granulocyte-macrophage)

Reference sequence