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pGL4-phFOSL1 (#RDB07476)

Promoter collection, Human FOSL1 promoter

Alternative name U2779-5
Clone info. PCR amplified human FOSL1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 65901742 to 65900422(NC_000011.10); relatively -1354 to -33, where +1 corresponds to 1 nt of NM_005438.5; relatively -1216 to +105, where +1 corresponds to 1 nt of NR_125339.1.
Comment PCR cloning, forward primer: CTTGGCCTTCCAAAGTGCTA; reverse primer: ATGAAAAGTTCTCGGGCTGA. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human FOS-like antigen 1 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07476.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07476 pGL4-phFOSL1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phFOSL1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07476).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12748_A4D2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12748_A4D2a.seq check
>07476_12748_A4D2_pGL4-4174F_C11_08.ab1
    1 GATGGGGGCC CAGAACAATT TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC
   61 GAGGATCTTG GCCTTCCAAA GTGCTAGGAT TACAAGCATG AGCCACCACA CCTGGCCCCA
  121 GCCCTCTTTC TGTAGTCGTG TGACAACTGA CAGCTCAGTA CTCCATCTGG GCCTCAGTTT
  181 CCTCATCTAT GATGGAAGTA ATCAGAGAGA AGCTTTGGGA TGGTGCCCAG CACTAACATT
  241 CTGGATGTGC GACAAGGTGT CTGTGAAGGT GTAAGGGCTT TGAAAATCTT GGCTGAGCCT
  301 GGGCTTGGAG TGCCCACGGC TGTGACTTGG CAACCTGTGC CAGCTACTCA CTTAGCGACT
  361 TCAAGCAATT CATTTCAGCA TCTCCCAGCC TCAGTTTCCC CATCTGTAAA ACAGGGATGA
  421 GGATGATACC TGCCTCACAT TCACTCACCT TTGACATACT CTCCAAACAA GTCTGGTGGC
  481 CACAAGCCTC CGAAAGGCAC TGACCGTAAT GAAGATGGCG TTTATCAGAC CTCAGGACAC
  541 CTGTAGTGGC CCTGAATTAA AACTCGTTAT AGCTCCTGAA ATTATCCTGA GTAATACTAT
  601 TATATTGCAT TTTATGTGGG GTTAGTTCAA AGCATTACCT TATCGCAAAC ATTTAAAATA
  661 TACCACCCAG CCTGGGCAAT ACGGCGAGAC CCAATCCCTA CAAAAAACAC AAAAATTACC
  721 TGGGTGTGGT GACGCGCGCC TGTAGTCCCA CTTACTTGGA GGGGGCGCTG AGGCAGGAGA
  781 ATCCCTTTAG CTCAGGAAGT TGAGCCTGCA GTGCGCCGAG ATCGAGCCAC TGCACTCCAG
  841 CCTGGGTGAC AGAGACCCGG TCTCAAAAAT ATAAAGTAAA ATAAAATATG CCACCATTTT
  901 TTGTAAACCC TGGTTTGTCA TTTATTCACT TTTTCATATA CTCGAAGCCT TAAAACAAGG
  961 CCAGTGGGAA AGACCTCACT CCCACGAAAG CTTTGGGGTG GGCGGTTGGC GTGGCTCCCT
 1021 AGAGATGTGA TTCTTTTCGT GCTATTTTTG TGGAGCAGAA ACCGGGAGGT TAGGCCCAGG
 1081 GCCTCGAGAG GGCTGGGGGC GGGCGCGGCT CTGGCAGAGC CGTCAGTCCG CAAGGGGGAA
1141 TCCCTGTAG
//
Primer:
Sequence file: RDB12748_A4D2b.seq check
>07476_12748_A4D2_pGL4-136R_D11_11.ab1
    1 GTTCCCAATG GTTTTGGGGC TCTTCCATGG TGGCTTTACC AACAGTAACC GGATTGCCAA
   61 GCTTGGCCGC CGAGGCCAGA TCTTGATATG AAAAGTTCTC GGGCTGAACC ACTGCGACCG
  121 CGGGGGGTGG GGGCGTTGCA GCGGCGCGGT CACCTCCAGG CTCCACCCCC GAGACGCGCC
  181 GCCTTGGCCC GTCGGTCCCG AACTTGGCGA CGCCTCCCCA AGTCCGGCTG CAGCCTTTTC
  241 ACCGGGATGG GCCCGGCTCG GCGTTCCCCG GCGGCTGCGG GCCCGCCCCC CTGACGTAGC
  301 TGCCCATACA TGGTGTAACT TCCTCGCCGC GCCCAGGTGG AGCCCCGGGT TCCCCTGCGG
  361 ACTGACGCAC CTGCCAGAGC CCGCGCCCCG CCCCAGCCCT CTCGAGGCCT GGGCTAACCT
  421 CCGTTTCTGC TCCCACAAAA TAGCACGAAA GAATCACATC TCTAGGAGCC ACGCCAACCG
  481 CCACCCAAAG CTTCGTGGAG TGAGGTCTTT CCACTGGCCT TGTTTTAAGG CTTCGAGTAT
  541 ATGAAAAGTG AATAAATGAC AAACCAGGGT TACAAAAATG GTGGCATATT TTATTTTACT
  601 TTATATTTTT GAGACCGGGT CTCTGTCACC CAGGCTGGAG TGCAGTGGCT CGATCTCGGC
  661 GCACTGCAGG CTCAACTTCC TGAGCTAAAG GGATTCTCCT GCCTCAGCGC CCCCTCCAAG
  721 TAAGTGGGAC TACAGGCGCG CGTCACCACA CCCAGGTAAT TTTTGTGTTT TTTGTAGGGA
  781 TTGGGTCTCG CCGTATTGCC CAGGCTGGGT GGTATATTTT AAATGTTTGC GATAAGGTAA
  841 TGCTTTGAAC TAAACCCCAC ATAAAAATGC AATATAATAG TATTACTCAG GATAATTTCA
  901 GGAGCTATAA ACGAGTTTTT AATTCAGGGC CACTACAGGT GTTCCTGAGG GTCTGATAAC
  961 GGCCATCTTC ATTACGGGTC AGTGCCCTTT CGGAGGCTTT GTGGGCCAAC CAGACTTTAT
 1021 TTGGAGAGTA TGTCAAAAGG TGAGGTGAAA TGTGAAGGCA GGGTATCATC CTCATTCCCT
 1081 GTTTTTAACA GAATGGGGAA AACTTGAAGC CTGAGATGCT GAATTGAAAT TGGCCTTTGG
1141 GAAAGATCGC AT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles