RDB07475 - pCMFlag_hTP53, P72R, V157F

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pCMFlag_hTP53, P72R, V157F (#RDB07475)

Expression vector, mammalian, of human TP53, derived from Lu-139 cell (RCB0469), Gateway(R) expression clone

Alternative name	Lu-139
Clone info.	Expression vector, mammalian, of human TP53, derived from Lu-139 cell (RCB0469), Gateway(R) expression clone. Expression was confirmed by Western blotting with anti-FLAG and anti-p53 antibodies.
Vector backbone	pCMV_S-FLAGc (RDB07396) (Plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human tumor protein p53 cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to use the MATERIAL only for academic research in the non-profit organization and to describe RIKEN BRC as the source of the MATERIAL in any publication.

Remarks An additional fee for license fee of Gateway(R) Expression Vector is required.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to use the MATERIAL only for academic research in the non-profit organization and to describe RIKEN BRC as the source of the MATERIAL in any publication.
Remarks	An additional fee for license fee of Gateway(R) Expression Vector is required. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。発表に際し、理研BRCから入手したことを明記すること。
備考	Gateway(R)発現ベクター。ライセンス料相当額を負担して頂きます。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07475	pCMFlag_hTP53, P72R, V157F	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hTP53, P72R, V157F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07475).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07475_A7Khp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB07475_A7Kha.seq
>07475_07475_A7Kh_CMV-Forward_G04_19_ABI24.ab1 1 TTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG 181 CGGCCATCAC AAGTTTGTAC AAAAAAGCAG GCTCGGAGGA GCCGCAGTCA GATCCTAGCG 241 TCGAGCCCCC TCTGAGTCAG GAAACATTTT CAGACCTATG GAAACTACTT CCTGAAAACA 301 ACGTTCTGTC CCCCTTGCCG TCCCAAGCAA TGGATGATTT GATGCTGTCC CCGGACGATA 361 TTGAACAATG GTTCACTGAA GACCCAGGTC CAGATGAAGC TCCCAGAATG CCAGAGGCTG 421 CTCCCCGCGT GGCCCCTGCA CCAGCAGCTC CTACACCGGC GGCCCCTGCA CCAGCCCCCT 481 CCTGGCCCCT GTCATCTTCT GTCCCTTCCC AGAAAACCTA CCAGGGCAGC TACGGTTTCC 541 GTCTGGGCTT CTTGCATTCT GGGACAGCCA AGTCTGTGAC TTGCACGTAC TCCCCTGCCC 601 TCAACAAGAT GTTTTGCCAA CTGGCCAAGA CCTGCCCTGT GCAGCTGTGG GTTGATTCCA 661 CACCCCCGCC CGGCACCCGC TTCCGCGCCA TGGCCATCTA CAAGCAGTCA CAGCACATGA 721 CGGAGGTTGT GAGGCGCTGC CCCCACCATG AGCGCTGCTC AGATAGCGAT GGTCTGGCCC 781 CTCCTCAGCA TCTTATCCGA GTGGAAGGAA ATTTGCGTGT GGAGTATTTG GATGACAGAA 841 ACACTTTTCG ACATAGTGTG GTGGTGCCCT ATGAGCCGCC TGAGGTTGGC TCTGACTGTA 901 CCACCATCCA CTACAACTAC ATGTGTAACA GTTCCTGCAT GGGCGGCATG AACCGGGAGG 961 CCCATCCTCA CCATCATCAC ACTGGTAAGA CTCCAGTGGT AATCTACTGG GACGGGAACA 1021 GCTTTGAGGT GCGTGTTGTG CCTGTCCTGG AGAGACCGGG CGCACAGAGA GGAGATCTCC 1081 GGCAGAAGGG GAGCTTCACT CACGAGCCTG CCTCTCCAGG GAGAGCCACC TTAAAAG //
Primer: BGH_rev2 Sequence file: RDB07475_A7Khb.seq
>07475_07475_A7Kh_BGH_rev2_H04_22_ABI24.ab1 1 CGATCAAGGA TCAGGTAGGC ACGGGGGAGG GGCAAACACA GATGGCTGGC AACTAGAAGG 61 CACAGTCGAG GCTGATCAGC GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC 121 TAGATGCATG CTCGAGCGGC CATCACCACT TTGTACAAGA AAGCTGGGTT CAGTCTGAGT 181 CAGGCCCTTC TGTCTTGAAC ATGAGTTTTT TATGGCGGGA GGTAGACTGA CCCTTTTTGG 241 ACTTCAGGTG GCTGGAGTGA GCCCTGCTCC CCCCTGGCTC CTTCCCAGCC TGGGCATCCT 301 TGAGTTCCAA GGCCTCATTC AGCTCTCGGA ACATCTCGAA GCGCTCACGC CCACGGATCT 361 GAAGGGTGAA ATATTCTCCA TCCAGTGGTT TCTTCTTTGG CTGGGGAGAG GAGCTGGTGT 421 TGTTGGGCAG TGCTCGCTTA GTGCTCCCTG GGGGCAGCTC GTGGTGAGGC TCCCCTTTCT 481 TGCGGAGATT CTCTTCCTCT GTGCGCCGGT CTCTCCCAGG ACAGGCACAA ACACGCACCT 541 CAAAGCTGTT CCGTCCCAGT AGATTACCAC TGGAGTCTTC CAGTGTGATG ATGGTGAGGA 601 TGGGCCTCCG GTTCATGCCG CCCATGCAGG AACTGTTACA CATGTAGTTG TAGTGGATGG 661 TGGTACAGTC AGAGCCAACC TCAGGCGGCT CATAGGGCAC CACCACACTA TGTCGAAAAG 721 TGTTTCTGTC ATCCAAATAC TCCACACGCA AATTTCCTTC CACTCGGATA AGATGCTGAG 781 GAGGGGCCAG ACCATCGCTA TCTGAGCAGC GCTCATGGTG GGGGCAGCGC CTCACAACCT 841 CCGTCATGTG CTGTGACTGC TTGTAGATGG CCATGGCGCG GAAGCGGGTG CCGGGCGGGG 901 GGTGTGGAAT CAACCCACAG CTGCACAGGG CAGGTCTTGG CCAGTTGGCA AAACATCTTG 961 TTGAGGGCAG GGGAGTACGT GCAAGTCACA GACTTTGGCT GTCCCAGAAA TGCAAGAAGC 1021 CCAGACGGAA ACCCGTAGCT GCCCTGGTAG TTTTCTGGTA GGGACAGAGA TGGACAGGGG 1081 CAGAGGGGGC TGTTGCAGGG ACCGTCGATG TAAGAGCTGG CTTGTTGCAG GGGTCCCACG 1141 CCCGAG //
Primer: SV40pro_F_V2 Sequence file: RDB07475_A7Khc.seq
>07475_07475_A7Kh_SV40pro_F_V2_A05_02_ABI24.ab1 1 CTCCACCTTT TTTGGGAGGC CTAGGCTTTT GCAAAAGCTC CCGGGAGCTT GGATATCCAT 61 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT 121 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA 181 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT 241 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT 301 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC 361 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT 421 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA 481 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG 541 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC 601 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC 661 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT 721 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA 781 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC 841 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG 901 ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT 961 TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTTCCGGG ACGCCCGGGC 1021 TGGATGATCC TTCCAGCGGC GGGGATCTCA TGCTGGAGTT CTTTCGCCCA CCCCCACTTT 1081 GTTTATTGCA GCCTTATATT GTACAATAAG GCAATAGCAT TCCACCAATT TTTCCACCAA //