RDB07474 - pCMFlag_hTP53, P72R, G244C, S315F

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pCMFlag_hTP53, P72R, G244C, S315F (#RDB07474)

Expression vector, mammalian, of human TP53, derived from Lu-135 cell (RCB0468), Gateway(R) expression clone

Alternative name	Lu-135
Clone info.	Expression vector, mammalian, of human TP53, derived from Lu-135 cell (RCB0468), Gateway(R) expression clone. Expression was confirmed by Western blotting with anti-FLAG and anti-p53 antibodies.
Vector backbone	pCMV_S-FLAGc (RDB07396) (Plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human tumor protein p53 cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The RECIPIENT agrees to use the MATERIAL only for academic research in the non-profit organization and to describe RIKEN BRC as the source of the MATERIAL in any publication.

Remarks An additional fee for license fee of Gateway(R) Expression Vector is required.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to use the MATERIAL only for academic research in the non-profit organization and to describe RIKEN BRC as the source of the MATERIAL in any publication.
Remarks	An additional fee for license fee of Gateway(R) Expression Vector is required. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。発表に際し、理研BRCから入手したことを明記すること。
備考	Gateway(R)発現ベクター。ライセンス料相当額を負担して頂きます。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07474	pCMFlag_hTP53, P72R, G244C, S315F	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hTP53, P72R, G244C, S315F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07474).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07474_A7Khp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB07474_A7Kha.seq
>07474_07474_A7Kh_CMV-Forward_D04_10_ABI24.ab1 1 CTTTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG 181 CGGCCATCAC AAGTTTGTAC AAAAAAGCAG GCTCGGAGGA GCCGCAGTCA GATCCTAGCG 241 TCGAGCCCCC TCTGAGTCAG GAAACATTTT CAGACCTATG GAAACTACTT CCTGAAAACA 301 ACGTTCTGTC CCCCTTGCCG TCCCAAGCAA TGGATGATTT GATGCTGTCC CCGGACGATA 361 TTGAACAATG GTTCACTGAA GACCCAGGTC CAGATGAAGC TCCCAGAATG CCAGAGGCTG 421 CTCCCCGCGT GGCCCCTGCA CCAGCAGCTC CTACACCGGC GGCCCCTGCA CCAGCCCCCT 481 CCTGGCCCCT GTCATCTTCT GTCCCTTCCC AGAAAACCTA CCAGGGCAGC TACGGTTTCC 541 GTCTGGGCTT CTTGCATTCT GGGACAGCCA AGTCTGTGAC TTGCACGTAC TCCCCTGCCC 601 TCAACAAGAT GTTTTGCCAA CTGGCCAAGA CCTGCCCTGT GCAGCTGTGG GTTGATTCCA 661 CACCCCCGCC CGGCACCCGC GTCCGCGCCA TGGCCATCTA CAAGCAGTCA CAGCACATGA 721 CGGAGGTTGT GAGGCGCTGC CCCCACCATG AGCGCTGCTC AGATAGCGAT GGTCTGGCCC 781 CTCCTCAGCA TCTTATCCGA GTGGAAGGAA ATTTGCGTGT GGAGTATTTG GATGACAGAA 841 ACACTTTTCG ACATAGTGTG GTGGTGCCCT ATGAGCCGCC TGAGGTTGGC TCTGACTGTA 901 CCACCATCCA CTACAACTAC ATGTGTAACA GTTCCTGCAT GTGCGGCATG AAACCGGAGG 961 CCCATCCTCA CCATCATCAC ACTGGGAAGA CTCCAGTGGT AATCTACTGG ACGGAACAGC 1021 TTTGAGTGCG TGTTGTGCCT GTCCTGGAGA GACCGCGCAC AGAGGAGGAG ATCCTCCGCA 1081 GAAGGGAAGT CTCACAACGA GCTGGCTCCC CAAGGGGGAG CCACCTATTA //
Primer: BGH_rev2 Sequence file: RDB07474_A7Khb.seq
>07474_07474_A7Kh_BGH_rev2_E04_13_ABI24.ab1 1 GGCTCAGCGT AGGTAACACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGAG CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG 121 ATGCATGCTC GAGCGGCCAT CACCACTTTG TACAAGAAAG CTGGGTTCAG TCTGAGTCAG 181 GCCCTTCTGT CTTGAACATG AGTTTTTTAT GGCGGGAGGT AGACTGACCC TTTTTGGACT 241 TCAGGTGGCT GGAGTGAGCC CTGCTCCCCC CTGGCTCCTT CCCAGCCTGG GCATCCTTGA 301 GTTCCAAGGC CTCATTCAGC TCTCGGAACA TCTCGAAGCG CTCACGCCCA CGGATCTGAA 361 GGGTGAAATA TTCTCCATCC AGTGGTTTCT TCTTTGGCTG GGGAAAGGAG CTGGTGTTGT 421 TGGGCAGTGC TCGCTTAGTG CTCCCTGGGG GCAGCTCGTG GTGAGGCTCC CCTTTCTTGC 481 GGAGATTCTC TTCCTCTGTG CGCCGGTCTC TCCCAGGACA GGCACAAACA CGCACCTCAA 541 AGCTGTTCCG TCCCAGTAGA TTACCACTGG AGTCTTCCAG TGTGATGATG GTGAGGATGG 601 GCCTCCGGTT CATGCCGCAC ATGCAGGAAC TGTTACACAT GTAGTTGTAG TGGATGGTGG 661 TACAGTCAGA GCCAACCTCA GGCGGCTCAT AGGGCACCAC CACACTATGT CGAAAAGTGT 721 TTCTGTCATC CAAATACTCC ACACGCAAAT TTCCTTCCAC TCGGATAAGA TGCTGAGGAG 781 GGGCCAGACC ATCGCTATCT GAGCAGCGCT CATGGTGGGG GCAGCGCCTC ACAACCTCCG 841 TCATGTGCTG TGACTGCTTG TAGATGGCCA TGGCGCGGAC GCGGGTGCCG GGCGGGGGTG 901 TGGAATCAAC CCACAGCTGC ACAGGGCAGG TCTTGGCCAG TTGGCAAAAC ATCTTGTTGA 961 GGGCAGGGGA GTACGTGCAA GTCACAGACT TGGCTGTCCC AGAATGCAAG AAGCCCAGAC 1021 GGAAACCGTA GCTGCCCTGG TAGTTTTCTG GGAGGGACAG AGATGGACAG GGTAAGAGGG 1081 GGCTGGTGCA TGGACGACGA TGTAGGAGCC TGCCTGATGC AGGGGACCAA CGCCGAGA //
Primer: SV40pro_F_V2 Sequence file: RDB07474_A7Khc.seq
>07474_07474_A7Kh_SV40pro_F_V2_F04_16_ABI24.ab1 1 GAGCAACTTT TTTGGAGGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGG ATATCCATTT 61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC 961 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGACGC CCGGCTGGGA 1021 TGATCCTTCC AGCGCGGGGA TCTCATGCTT GGAGTTCTTC CGCCCACCCC AACCTTGTTT 1081 AATGCAGCCT ATATTGTTAC AATAAAGCCA ATAGCATTCA CAAAACTTTT CAGA //