RDB07473 - pCMFlag_hTP53, G244S

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pCMFlag_hTP53, G244S (#RDB07473)

Expression vector, mammalian, of human TP53, derived from OCUB-M cell (RCB0881), Gateway(R) expression clone

Alternative name	OCUB-M
Clone info.	Expression vector, mammalian, of human TP53, derived from OCUB-M cell (RCB0881), Gateway(R) expression clone. Expression was confirmed by Western blotting with anti-FLAG and anti-p53 antibodies.
Vector backbone	pCMV_S-FLAGc (RDB07396) (Plasmid)
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human tumor protein p53 cDNA
Depositor\|Developer	DNA Bank, \| Kishikawa, Shotaro \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to use the MATERIAL only for academic research in the non-profit organization and to describe RIKEN BRC as the source of the MATERIAL in any publication.

Remarks An additional fee for license fee of Gateway(R) Expression Vector is required.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to use the MATERIAL only for academic research in the non-profit organization and to describe RIKEN BRC as the source of the MATERIAL in any publication.
Remarks	An additional fee for license fee of Gateway(R) Expression Vector is required. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。発表に際し、理研BRCから入手したことを明記すること。
備考	Gateway(R)発現ベクター。ライセンス料相当額を負担して頂きます。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07473	pCMFlag_hTP53, G244S	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hTP53, G244S was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07473).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07473_A7Khp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB07473_A7Kha.seq
>07473_07473_A7Kh_CMV-Forward_A04_01_ABI24.ab1 1 TTTTTCAACT CCGCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC 121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA 181 GGCGGCCATC ACAAGTTTGT ACAAAAAAGC AGGCTCGGAG GAGCCGCAGT CAGATCCTAG 241 CGTCGAGCCC CCTCTGAGTC AGGAAACATT TTCAGACCTA TGGAAACTAC TTCCTGAAAA 301 CAACGTTCTG TCCCCCTTGC CGTCCCAAGC AATGGATGAT TTGATGCTGT CCCCGGACGA 361 TATTGAACAA TGGTTCACTG AAGACCCAGG TCCAGATGAA GCTCCCAGAA TGCCAGAGGC 421 TGCTCCCCCC GTGGCCCCTG CACCAGCAGC TCCTACACCG GCGGCCCCTG CACCAGCCCC 481 CTCCTGGCCC CTGTCATCTT CTGTCCCTTC CCAGAAAACC TACCAGGGCA GCTACGGTTT 541 CCGTCTGGGC TTCTTGCATT CTGGGACAGC CAAGTCTGTG ACTTGCACGT ACTCCCCTGC 601 CCTCAACAAG ATGTTTTGCC AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC 661 CACACCCCCG CCCGGCACCC GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT 721 GACGGAGGTT GTGAGGCGCT GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC 781 CCCTCCTCAG CATCTTATCC GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG 841 AAACACTTTT CGACATAGTG TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG 901 TACCACCATC CACTACAACT ACATGTGTAA CAGTTCCTGC ATGAGCGGCA TGAACCGGGA 961 GGCCCATCCT CACCATCATC ACACTGGAAA GACTCCAGTG GTAATCTACT GGGACGGGAA 1021 CAGCTTTGAG GTGCGTGTTT GTGCCTGTCC TGGGGAGAGA CGGGCGCACA GAAGGAGAGA 1081 TCTCGGCAGA AGGGAAGTCC TCAATCACGA AGCCTGCTCC TCTCCAGGGG GGGAGCAACT 1141 A //
Primer: BGH_rev2 Sequence file: RDB07473_A7Khb.seq
>07473_07473_A7Kh_BGH_rev2_B04_04_ABI24.ab1 1 CGATTCAGAG TCACGGTACG CACGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA 61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC 121 TCTAGATGCA TGCTCGAGCG GCCATCACCA CTTTGTACAA GAAAGCTGGG TTCAGTCTGA 181 GTCAGGCCCT TCTGTCTTGA ACATGAGTTT TTTATGGCGG GAGGTAGACT GACCCTTTTT 241 GGACTTCAGG TGGCTGGAGT GAGCCCTGCT CCCCCCTGGC TCCTTCCCAG CCTGGGCATC 301 CTTGAGTTCC AAGGCCTCAT TCAGCTCTCG GAACATCTCG AAGCGCTCAC GCCCACGGAT 361 CTGAAGGGTG AAATATTCTC CATCCAGTGG TTTCTTCTTT GGCTGGGGAG AGGAGCTGGT 421 GTTGTTGGGC AGTGCTCGCT TAGTGCTCCC TGGGGGCAGC TCGTGGTGAG GCTCCCCTTT 481 CTTGCGGAGA TTCTCTTCCT CTGTGCGCCG GTCTCTCCCA GGACAGGCAC AAACACGCAC 541 CTCAAAGCTG TTCCGTCCCA GTAGATTACC ACTGGAGTCT TCCAGTGTGA TGATGGTGAG 601 GATGGGCCTC CGGTTCATGC CGCTCATGCA GGAACTGTTA CACATGTAGT TGTAGTGGAT 661 GGTGGTACAG TCAGAGCCAA CCTCAGGCGG CTCATAGGGC ACCACCACAC TATGTCGAAA 721 AGTGTTTCTG TCATCCAAAT ACTCCACACG CAAATTTCCT TCCACTCGGA TAAGATGCTG 781 AGGAGGGGCC AGACCATCGC TATCTGAGCA GCGCTCATGG TGGGGGCAGC GCCTCACAAC 841 CTCCGTCATG TGCTGTGACT GCTTGTAGAT GGCCATGGCG CGGACGCGGG TGCCGGGCGG 901 GGGTGTGGAA TCAACCCACA GCTGCACAGG GCAGGTCTTG GCCAGTTGGC AAAACATCTT 961 GTTGAGGGCA GGGGAGTACG TGCAAGTCAC AGACTTGGCT GTCCCAGAAT GCAAGAAGCC 1021 CAGACGGGAA ACCGTAGCTG CCCTGGTAGT TTTTCTGGGA GGACAGAGAA TGGACAGGGG 1081 TAGAGGGGGC TGATGCAGGA CGACGGATGT AAGGAAGCCT GCCTGCTGCA GGGGCTCACA 1141 CGAAG //
Primer: SV40pro_F_V2 Sequence file: RDB07473_A7Khc.seq
>07473_07473_A7Kh_SV40pro_F_V2_C04_07_ABI24.ab1 1 GTACCCGCTT TTTGGAGGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGG ATATCCATTT 61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC 961 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCCGGGAC GCCGGGCTGG 1021 ATGATCCCTC CCAGCGCGGG GGATCTCATG CTGGAGTTTC TTTCGTCCAA CCCCAACTTT 1081 GTTAATTGCA GCTATATGGT ACCAATAAGG CATAGCAATC ACAAAATTTT CCACCAGA //