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pGL4-phSESN2

Promoter collection, Human SESN2 promoter

Catalog number RDB07413
Resource name pGL4-phSESN2
Alternative name U3307-10
Clone info. PCR amplified human SESN2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 28258366 to 28259686(NC_000001.11); relatively -1152 to +169, where +1 corresponds to 1 nt of NM_031459.5.
PCR cloning, forward primer: AAGTGCCTTGGGAGCTCAG; reverse primer: CGCTTTGGTGCTGGACTCT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human sestrin 2 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human sestrin 2

          Reference sequence
            

          Distribution information

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          RDB07413 pGL4-phSESN2 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07413.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07413_A1Aqp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: pause site, insert 5'
          Sequence file: RDB07413_A1Aqa.seq check
          >07413_07413_B1K5F_pGL4ins_F_A09_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNANN AGGCTGTCCC CAGTGCAAGT GCAGGTGCCA
             61 GAACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG GATAAGTGCC
            121 TTGGGAGCTC AGGGGAGGGT GAGATTAATC TCTTTTTCCC AAGAATCTGG AGGCAGGAAA
            181 CCAAGTCGGT TTCATCTTTG GAATTGTAAG CTTAGACTTA GAAGTCTCAG AGGGGGACTT
            241 GGGGGTCTTA TCCACCCTCA CCCCTGGCAG GATTCCCTGT CTAGAATAAA CAATAACTTT
            301 AAAATGGAAA CAAAAAAAGG AGCTCTGAAG GAAAACTGTG AAGGAAGAGG CTCCGTTTGA
            361 AATGAATAGA AAATGAATTT AGCTTGGAGG TGCTGAGGTG TTGGTAGAGG CAACTTTCTG
            421 GCAAGAGGCG AGTTGAAGGC CAGGAAGTGA ATGAGATCAG GGAGAGTGTC TGGAGGGAAG
            481 AGGGGCAAAA ACAAACTGGG GAAGCACCCA CGTTTTAGGA AGTGGGTGGT GAAAGAGGAG
            541 TCAGAGAAGG CCAGAGGGAG AGGTGAGCAA AGATCCAGCA GAACACCTTG TTACAAAGTT
            601 TCGAGGAGGG TGGGAGGGGT GGAAGCCAGG ACGGGTAGCA GAAGGAAGAA AACTTCCAGC
            661 AATAGGGATT GAGGTTCCAC ACGAAGTTAC CAATGACATT TCAAGNNAAT GAAGGCTTTT
            721 CCTGANGCTC CACAAATAAN GTGAATTCCC TGTGTCTCGC ATCTTTGGAG CTTCCCCGAG
            781 GCCAGGGGGA GCAGGGTTGT GCCNTCTGAN CTCTAAATAT CTGGGGGTNC AGTTCTATAA
            841 TTAGCANCAC CGGATANGAC GCGAGCCAAN NNTCNCTTAN CCCTANCCAG TCCCTCNNGG
            901 AACTGAAAAN GATGANNNAN TTCATTTAGG AGANTTNCAC ANNCNGCNNT TTNGGNCTNN
          961 NNNNNNGNAN NNNCNNTTTN NATCANNNNN NCCCNAANGN NNNNNNNNN
          //
          Primer: pGL4ins_R
          Region: luc 5',insert 3'
          Sequence file: RDB07413_A1Aqb.seq check
          >07413_07413_B1K5F_pGL4ins_R_B09_02_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNNNGNGCT GGGCCCTTCT TANTGTTTTT
             61 GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC CGCCGAGGCC
            121 AGATCTTGAT CGCTTTGGTG CTGGACTCTT CCCCCAGTCG GGCTTCGGGA ACGGCGAACG
            181 GCCTCGGGGG CCTGGGAAGG GCCGCAGCGC AGGGGTTTTC ACGGCCTCGG AAGTCCGCAG
            241 GGGCCAGGGC TGCTCCCGGA CCCAGCGCTC CTCAGCATCC CCGCCGCGGA ACTGCCAGCG
            301 CTCGCCGGGC TCTGACACCA GCAGTTCAGC CCGCTGCGGT CCCCGGGGGA GGGCGGGGAC
            361 ACAAAAGCCC GGCGGTCCAG CCAATCAGAG GTCTGTTTCC CCGGGGAGGC GGGGGAAACT
            421 CCGCCCCCTG GAGTCACGGG CTCCGCCAAT CAGCACCGGG GAGCGCCCGG AATTCTGGGA
            481 GTTGTAGTCC GCCTCGGGTG AATGCTGCAA ACTTTTGCAA GACGGTTTAG TCAGATCCCT
            541 GGCAACACCA GACGGCAGTG ACAGCCCTAA GAGTCATTAG GGTTGCGTGA TGAAAAGGGA
            601 CTTCACTGGG CTAGGCNNNN NNGCCGAGTG TGAAATCTCC TAAATGAATT CTTCATCTTT
            661 TCAGTTCCTG GAGGGACTGG CTAGGGTTAA GAGAGGTTTG GCTCGCGTCC TATCCGGTGC
            721 TGCTAATTAT AGAACTGAAC CCCANATATT TAGAGGTCAG ANGGCACAAC CCTGCTCCCC
            781 CTGGCCTCGG GGAAGCTCCA AAGATGCGAG ACNCAGGGAA TTCACCTTAT TTGTGGANCC
            841 TCAGGAAAAG CCTTNCATTT NCTTGAANNG TCNNTNGNAA CTNNGNGTGN NANNNTCNAT
            901 CCCTATNGCT GGAAGTTTTC TTCCTNNTGC TACCCGTNCN NNGCNNNNNN NCCCTCCCNN
            961 CCNTNNNNGN ANNNNTTNNN NANNNNNGNN GNTTCNNNNN NGNANCTTTN GNNNCNNNTN
          1021 NNCNNNNNNN NCNNNNNNNN NN
          //
          Primer: SV40pA_R
          Region: SV40 pA,luc 3'
          Sequence file: RDB07413_A1Aqc.seq check
          >07413_07413_B1K5F_SV40pA_R_C09_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNAAAGCAT TTTTTNNNNN GCATTCTANN
             61 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC
            121 CCCGACTCTA GAATTATTAC ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT
            181 CGCGGATCTT GCGGGCGTCC AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA
            241 CAACACCACC GCGCAGCTTC TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT
            301 CCTTCTCGGT CATGGTTTTA CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG
            361 CATCGTCGTC GGGCAGGCCG GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA
            421 TGCTCTCCAG TTCGGCTGGG GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC
            481 GGTCCACGAT GAAGAAGTGC TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC
            541 AGCCGTCCTT GTCGATGAGA GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA
            601 TCATGGGGCC ACGGACGCAC AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT
            661 CCAAGTCCAC CACCTTAGCC TCGAAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG
            721 TCGTCCCCTT CNGGGGTGAT CAGAATGGCG CTGGTTGNTT CTGTCAGGCC GTANCCCTGG
            781 CGGATGCCTG GNANGTGGAA GCGTTTGGNC NCNGCCTCAC CTACCTCCTT GCTGAGCGGC
            841 NNCCCGCCNG CTGGCGATCT CGTGCAAGTT GCTTANGTCG TACTTGNCNA NNNANAGTGC
            901 TCNTANNGAA NNANCTAANN NNTGNGGGNC NNCNNCAGGG CAGANTGANT CTTATNNTCT
          961 TGCAAGCTGC GCNNNNAANN NNCTNCNNNN NNNNNANNNG NTNNNNNNAN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user report Park, S.J., Protective effect of sestrin2 against iron overload and ferroptosis-induced liver injury. Toxicol. Appl. Pharmacol. 379: 114665 (2019). PMID 31323261.
          user report Jegal, K.H., Activating transcription factor 6-dependent sestrin 2 induction ameliorates ER stress-mediated liver injury. Biochim. Biophys. Acta 1864 (7): 1295-1307 (2017). PMID 28433684.
          user report Reddy, K., Dysregulation of Nutrient Sensing and CLEARance in Presenilin Deficiency. Cell Rep. 14 (9): 2166-2179 (2016). PMID 26923592.
          user report Jegal, K.H., Eupatilin induces Sestrin2-dependent autophagy to prevent oxidative stress. Apoptosis 21 (5): 642-656 (2016). PMID 27015669.
          user report Jin, S.H., Resveratrol inhibits LXR alpha-dependent hepatic lipogenesis through novel antioxidant Sestrin2 gene induction. Toxicol. Appl. Pharmacol., 271 (1): 95-105 (2013). PMID 23651738.
          user report Yang, J.H., Isorhamnetin protects against oxidative stress by activating Nrf2 and inducing the expression of its target genes. Toxicol. Appl. Pharmacol., 274 (2): 293-301 (2013). PMID 24211276.
          user report Shin, B.Y., Nrf2-ARE pathway regulates induction of Sestrin-2 expression. Free Radic. Biol. Med., 53 (4): 834-841 (2012). PMID 22749810.

          2021.12.02

          GNP_filter3_RDBDEP_html_211111.pl