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pGL4-phSESN2

Promoter collection, Human SESN2 promoter

Catalog number RDB07413
Resource name pGL4-phSESN2
Alternative name U3307-10
Clone info. Promoter collection. PCR amplified human SESN2 promoter sequence was inserted into a firefly luciferase vector. Genomic DNA corresponding to 1 to 235nt and approx.1.1 kb up-stream of NM_031459.4 (28,258,366-28,259,686 of NC_000001.11) was cloned.
F-primer: AAGTGCCTTGGGAGCTCAG; R-primer: CGCTTTGGTGCTGGACTCT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human sestrin 2 genomic DNA
Depositor DNA Bank, |
 
Other clones in our bank human SESN2 (NCBI Gene 83667) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07413 pGL4-phSESN2 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file PDF RDB07413.pdf from Depositor

Featured content

Featured content Promoter Collection (Firefly Luciferase) (English text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07413_A3Irp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F RDB07413_A3Ira.seq
>07413_07413_A3Ir_pGL4-4174F_A01_01.ab1
    1 CTTTGGGCGG ACAATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
   61 ATAAGTGCCT TGGGAGCTCA GGGGAGGGTG AGATTAATCT CTTTTTCCCA AGAATCTGGA
  121 GGCAGGAAAC CAAGTCGGTT TCATCTTTGG AATTGTAAGC TTAGACTTAG AAGTCTCAGA
  181 GGGGGACTTG GGGGTCTTAT CCACCCTCAC CCCTGGCAGG ATTCCCTGTC TAGAATAAAC
  241 AATAACTTTA AAATGGAAAC AAAAAAAGGA GCTCTGAAGG AAAACTGTGA AGGAAGAGGC
  301 TCCGTTTGAA ATGAATAGAA AATGAATTTA GCTTGGAGGT GCTGAGGTGT TGGTAGAGGC
  361 AACTTTCTGG CAAGAGGCGA GTTGAAGGCC AGGAAGTGAA TGAGATCAGG GAGAGTGTCT
  421 GGAGGGAAGA GGGGCAAAAA CAAACTGGGG AAGCACCCAC GTTTTAGGAA GTGGGTGGTG
  481 AAAGAGGAGT CAGAGAAGGC CAGAGGGAGA GGTGAGCAAA GATCCAGCAG AACACCTTGT
  541 TACAAAGTTT CGAGGAGGGT GGGAGGGGTG GAAGCCAGGA CGGGTAGCAG AAGGAAGAAA
  601 ACTTCCAGCA ATAGGGATTG AGGTTCCACA CGAAGTTACC AATGACATTT CAAGGAATGA
  661 AGGCTTTTCC TGAGGCTCCA CAAATAAGGT GAATTCCCTG TGTCTCGCAT CTTTGGAGCT
  721 TCCCCGAGGC CAGGGGGAGC AGGGTTGTGC CCTCTGACCT CTAAATATCT GGGGTTCAGT
  781 TCTATAATTA GCAGCACCGG ATAGGACGCG AGCCAAACCT CTCTTAACCC TAGCCAGTCC
  841 CTCCAGGAAC TGAAAAGATG AAGAATTCAT TTAGGAGATT TCACACTCGG CTGTTTGGCC
  901 TAGCCCAGTG AAGTCCCTTT TCATCACGCA ACCCTAATGA CTCTTAAGGG CTGTCACTGC
  961 CGTCTGGTGT TGCCAGGGAT CTGACTAAAC CGGTCTGCAA AAGTTTGCAG CAATTCCACC
 1021 CGGAGCGGAC TTACACTCCC CAGATTCGGA CGCTCCCCCG ATGCTGAATG GCGAGCCGTG
1081 AACTCCAGGG ACGGGATTTT TCCCCACGCG
//
Primer: pGL4-136R RDB07413_A3Irb.seq
>07413_07413_A3Ir_pGL4-136R_B01_04.ab1
    1 GCTAGCTTTT GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
   61 CGCCGAGGCC AGATCTTGAT CGCTTTGGTG CTGGACTCTT CCCCCAGTCG GGCTTCGGGA
  121 ACGGCGAACG GCCTCGGGGG CCTGGGAAGG GCCGCAGCGC AGGGGTTTTC ACGGCCTCGG
  181 AAGTCCGCAG GGGCCAGGGC TGCTCCCGGA CCCAGCGCTC CTCAGCATCC CCGCCGCGGA
  241 ACTGCCAGCG CTCGCCGGGC TCTGACACCA GCAGTTCAGC CCGCTGCGGT CCCCGGGGGA
  301 GGGCGGGGAC ACAAAAGCCC GGCGGTCCAG CCAATCAGAG GTCTGTTTCC CCGGGGAGGC
  361 GGGGGAAACT CCGCCCCCTG GAGTCACGGG CTCCGCCAAT CAGCACCGGG GAGCGCCCGG
  421 AATTCTGGGA GTTGTAGTCC GCCTCGGGTG AATGCTGCAA ACTTTTGCAA GACGGTTTAG
  481 TCAGATCCCT GGCAACACCA GACGGCAGTG ACAGCCCTAA GAGTCATTAG GGTTGCGTGA
  541 TGAAAAGGGA CTTCACTGGG CTAGGCCAAA CAGCCGAGTG TGAAATCTCC TAAATGAATT
  601 CTTCATCTTT TCAGTTCCTG GAGGGACTGG CTAGGGTTAA GAGAGGTTTG GCTCGCGTCC
  661 TATCCGGTGC TGCTAATTAT AGAACTGAAC CCCAGATATT TAGAGGTCAG AGGGCACAAC
  721 CCTGCTCCCC CTGGCCTCGG GGAAGCTCCA AAGATGCGAG ACACAGGGAA TTCACCTTAT
  781 TTGTGGAGCC TCAGGAAAAG CCTTCATTCC TTGAAATGTC ATTGGTAACT TCGTGTGGAA
  841 CCTCAATCCC TATTGCTGGA AGTTTTCTTC CTTCTGCTAC CCGTCCTGGC TTCCACCCCT
  901 CCCACCCTCC TCGAAACTTT GTAACAAGGT GTTCTGCTGG ATCTTTGCTC ACCTCTCCCT
  961 CTGGCCTTCT CTGACTCCTC TTTCACCACC CACTTCCTAA AAACGTGGTG CTTTCCCCCA
 1021 GTTTGTTTTT GGCCCCTTCG TCCTCCAAGA CACTCTCCAT GATCTCAATT TCACATCCTG
1081 GACCTTTCGA GCG
//
Primer: pAxCALNL_F1 RDB07413_A3Irc.seq
>07413_07413_A3Ir_pAxCALNL_F1_A04_01.ab1
    1 TATTATCTTA TCATGTCTGC TCGAGCGGCC GGCCGCCCCG ACTCTAGAAT TATTACACGG
   61 CGATCTTGCC GCCCTTCTTG GCCTTAATGA GAATCTCGCG GATCTTGCGG GCGTCCAACT
  121 TGCCGGTCAG TCCTTTAGGC ACCTCGTCCA CGAACACAAC ACCACCGCGC AGCTTCTTGG
  181 CGGTTGTAAC CTGGCTGGCC ACATAGTCCA CGATCTCCTT CTCGGTCATG GTTTTACCGT
  241 GTTCCAGCAC GACGACTGCG GCGGGCAGCT CGCCGGCATC GTCGTCGGGC AGGCCGGCGA
  301 CCCCGGCGTC GAAGATGTTG GGGTGTTGCA GCAGGATGCT CTCCAGTTCG GCTGGGGCTA
  361 CCTGGTAGCC CTTGTATTTG ATCAGGCTCT TCAGCCGGTC CACGATGAAG AAGTGCTCGT
  421 CCTCGTCCCA GTAGGCGATG TCGCCGCTGT GCAGCCAGCC GTCCTTGTCG ATGAGAGCGT
  481 TTGTAGCCTC GGGGTTGTTA ACGTAGCCGC TCATGATCAT GGGGCCACGG ACGCACAGCT
  541 CGCCGCGCTG GTTCACACCC AGTGTCTTAC CGGTGTCCAA GTCCACCACC TTAGCCTCGA
  601 AGAAGGGCAC CACCTTGCCT ACTGCGCCAG GCTTGTCGTC CCCTTCGGGG GTGATCAGAA
  661 TGGCGCTGGT TGTTTCTGTC AGGCCGTAGC CCTGGCGGAT GCCTGGTAGG TGGAAGCGTT
  721 TGGCCACGGC CTCACCTACC TCCTTGCTGA GCGGCGCCCC GCCGCTGGCG ATCTCGTGCA
  781 AGTTGCTTAG GTCGTACTTG TCGATGAGAG TGCTCTTAGC GAAGAAGCTA AATAGTGTGG
  841 GCACCAGCAG GGCAGATTGA ATCTTATAGT CTTGCAAGCT GCGCAAGAAT AGCTCCTCCT
  901 CGAAGCGGTA CATGAGCACG ACCCGAAAGC CGCAGATCAA GTAGCCCAGC GTGGTGAACA
  961 TGCCCGAAGC CGTGGTGAAA TGGCACCACG CTGAGGATAG CGGTGTCGGG GGATGATCTG
 1021 CTTGCCCGAA GATGGGGGTT CGCGGGCATG ACTGAATCGG ACACAAGCGA TGGCGATGCC
1081 GGTAGGACTT ACGTCCATAG GTCAATCAGG TTACATGTGC CTCTCAT
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Jegal, K.H., Activating transcription factor 6-dependent sestrin 2 induction ameliorates ER stress-mediated liver injury. Biochim. Biophys. Acta 1864 (7): 1295-1307 (2017). PMID 28433684.
user report Reddy, K., Dysregulation of Nutrient Sensing and CLEARance in Presenilin Deficiency. Cell Rep. 14 (9): 2166-2179 (2016). PMID 26923592.
user report Jegal, K.H., Eupatilin induces Sestrin2-dependent autophagy to prevent oxidative stress. Apoptosis 21 (5): 642-656 (2016). PMID 27015669.
user report Jin, S.H., Resveratrol inhibits LXR alpha-dependent hepatic lipogenesis through novel antioxidant Sestrin2 gene induction. Toxicol. Appl. Pharmacol., 271 (1): 95-105 (2013). PMID 23651738.
user report Yang, J.H., Isorhamnetin protects against oxidative stress by activating Nrf2 and inducing the expression of its target genes. Toxicol. Appl. Pharmacol., 274 (2): 293-301 (2013). PMID 24211276.
user report Shin, B.Y., Nrf2-ARE pathway regulates induction of Sestrin-2 expression. Free Radic. Biol. Med., 53 (4): 834-841 (2012). PMID 22749810.

2018.12.10

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