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pGL4-phPTEN (#RDB07400)

Promoter collection, Human PTEN promoter

Alternative name U2733(2111)-8
Clone info. PCR amplified human PTEN promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 87862065 to 87863506(NC_000010.11); relatively -1560 to -118, where +1 corresponds to 1 nt of NM_000314.7.
Comment PCR cloning, forward primer: cgtgtttggatgtgggtgct; reverse primer: gcctcggaagaccgaggggag. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human phosphatase and tensin homolog genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07400.pdf

Distribution information

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RDB07400 pGL4-phPTEN DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPTEN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07400).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07400_A9Gvp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F ()
Region: pause site,insert5'
Sequence file: RDB07400_A9Gva.seq check
>07400_07400_A9Gv_1_pGL4ins_F_G10_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNANNNN NNAGNCTGTC CCCNGTGCAA GTGCAGGTGC CAGAACATTT
   61 CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG AGGATCGTGT TTGGATGTGG
  121 GTGCTTGTGT AACCAGTTCC CCAAGCGCCA GCCCCGACAG CGCTCCTTCG GGAGGCTGGT
  181 CCGAGCCCCT GTTTCCGCCG CGGCGCAGGA AGGGTTGGGG TTCCGCTGCC TGCACCAGGC
  241 AAGAGCACCC CGAGCAAAGG AAGAAGACGA CTTGCCTCCG GAGCTATCAC TGGGGAGTGG
  301 GAATTTGGAA AGTTCCCCAA CTAGGGACAC ACGTGACCTC CTTCGGAAAG TAGTTCCGAC
  361 TGTGGCCCGT GTATCCTTCC ACCTCCTTTT GAACCCTCCT AGGTCTCCTC GCCCCGCCCA
  421 CTCGCTGGGC TGCAGCTTCC TACCGTTCCG TACTTTCCAC TCAACCCGGT AACCCCAAAC
  481 GTGCACGGTC CGGCCGGGGC GCGCGGAGCC TGGCCCCGGG CGATCCATCC TGCCGGGTTT
  541 TCACGGCGGC CAAGGGGGGG CGGGGCTAGG TGGTCTCTGA GAACCGAGCT TGACTCCGAC
  601 GCCGCGAACC GACCTGGAGC CCGAGGGGAA AGATGCTCGA CTCTCTTGGG GGCACCGGAG
  661 CGGGCGCAGG AGAGGCCTGC GGGGTGCGTC CCACTCACAG GGNTCCTCTT TCAGTTCATT
  721 TAGATAGGTG CCCTTTGGGC CCTTGAAATT CAACGGCTAT GTGTTCACGT TCAGCACGCT
  781 CGGCTGANAG CTTTCATTTT TAGGGCAAAC GAGCCGANTT ACCGGGGGAA NCGAGANGTG
  841 GGGGCGCNNC AANGGNNCCG GANGANGNGA TACACGCNGG CGANACAATA NCANGNTNCN
901 CTTNNNGCTN ANNNNGACAC NNTNNNGNNN CNNNNTNNGG GGGAANGGGG G
//
Primer: pGL4-136R ()
Region: insert3'
Sequence file: RDB07400_A9Gvb.seq check
>07400_07400_A9Gv_1_pGL4-136R_E01_13_ABI24.ab1
    1 NNTGCCNAGC TTGGCCGCCG AGGCCAGATC TTGATGCCTC GGAAGACCGA GGGGAGGCGG
   61 GAGGCGAGCG AGAGGCGGAC GGGACCGCGC CGGGCGAGGG GAGGGCAGGG CAGGGCAGGG
  121 GGCGGTAGGA GGGGGCAGAG CGGTAGCTCT GGGTGCGAGC GCAGAGTCCC CAAGCCGCAG
  181 GCTCTACTGA GCATGCCCAG TGTAGCTGCC TGGGGCTTGC TCGGGCCGGT TCCCAGCCGC
  241 CAGCCTGCAG CTGCACTTGC TGCGGCTTTT GCAGCAACGC GAGGCGAGGA TAACGAGCTA
  301 AGCCTCGGCC TCTGCCCAGA AACCCAGCCG GAGGCAGGGT AGGCTGTTGT GGGGCGGGGG
  361 TGGAGGACTG ATGATGAAAG CTGAGATGGG TGCGTTGAGC AGTGTCACTG ACTCGAGTCT
  421 GAGGTTACCT GTGCACAGGT GAAAAGGACC AGGTGACCAC GCTGCTCAGT GTAGAGGGAA
  481 ATGCAGGGAC GGTCCCTGCA AGGGGAATAC CCTCCCCCCT TGCCTCTACC CCTAGATTTC
  541 CGCGGCGTGG ACTGCATTCG CTCTTTCCTT TTGCACCGCT GTCGGATCAC AATCGTTCGC
  601 AGAGNTTTGA CTGTAACAGC CTTTGCCTAG AGATTCCCCC TTCCCCCAAA TCTGTGTCCT
  661 CATGGTGTCA GTCTTAGCAC AAAGAGCAAC CTGCTATTGT GTCGCCAGCG TGTATCACCT
  721 CATCCGGCTC CCTTGCAGCG CCCCACCTCT CGCTNCCCCG GTAACTCGGC TCGTTNGCCC
781 TAAAAATGAA AGCTCTCAN
//
Primer: pGL4pA_R ()
Region: SV40a(A),Luc
Sequence file: RDB07400_A9Gvc.seq check
>07400_07400_A9Gv_1_pGL4pA_R_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TNNTCGNNTT NNCNNCNTTT GTAGAGGTTT TACTTGCTTT
   61 AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT
  121 TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC
  181 AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC
  241 TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC ACGGCGATCT
  301 TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC AACTTGCCGG
  361 TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC TTGGCGGTTG
  421 TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA CCGTGTTCCA
  481 GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG GCGACCCCGG
  541 CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG GCTACCTGGT
  601 AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC TCGTCCTCGT
  661 CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA GCGTTTGTAG
  721 CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC AGCTCGCCGC
  781 GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC TCGAANAANG
  841 GCACCACCTT GCCTACTGCG CNNGGCTTGT CGTCCCNTTC GGGGGGTGAT CAGAATGGCG
901 CTNGNTGNTT CTGTCAGGCC GNANCCNNNG NGGANGNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles