RDB07388 - pGL4-phHLA-E

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pGL4-phHLA-E (#RDB07388)

Promoter collection, Human HLA-E promoter

Alternative name	U2649 (2027)-3
Clone info.	PCR amplified human HLA-E promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 30488374 to 30489534(NC_000006.12); relatively -1135 to +26, where +1 corresponds to 1 nt of NM_005516.6.
Comment	PCR cloning, forward primer: tctcagcctccagagttgct; reverse primer: catgatcccagcctctgagt. Entire sequence of promoter region has not been confirmed. Known differences (at least): Insertion of A before 30,488,837nt of NC_000006.12.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human major histocompatibility complex, class I, E genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07388.pdf

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07388	pGL4-phHLA-E	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phHLA-E was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07388).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07388_A7Eop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: RDB07388#2 Sequence file: RDB07388_A7Eoa.seq
>07388_07388_A7Eo_RDB07388#2_2_E10_05_ABI08.ab1 1 CCGGACCAAA AGAAAAAGAA ATTAGTGAGG ACCCAAATAA ATTTTGTTTC TAGTGGATTG 61 TATCAATTTT TATCATATAT AAATTAAAAG ATATTAAAAC AAAATATTAA AAATATTAAT 121 TCATTTAAGA GAATATTAAT AACCCATTAC ATGTTAATAG AAACAACTTT TTTTTTTTTT 181 TTGAGTCAGA GTCTTGCTCT GTCCCCAGGC TGGAGTGCAG TGGCATGATC TCGGCTCACT 241 GCAACCTCCT TCTCCCGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCCA GTAACTGGAA 301 CTACAGGCGT GTGCCACCAC ACCCAGCTAA TTTTTGTGTT TTTAGTAGAG ACGGGGTTTC 361 ACCATGTTGG CCAGGATGGT CTTGATCTCT TGATCGGAAA TAACATATCT TTAATGAAAA 421 AAAACTTAAT TTTTAATTTT TCCAAAATAA ACAACGTATA GGCCGGGAAC GGTGGCTCAC 481 GCCTGTAATC GCAGCATTTT GGTAGGCCGA GGCAGGCAGA TCACGAGGTC AGGAGTTCAA 541 GACTAACCTG GCCAACATGA TGAAACCCTG TCTCTACTAA CAATACAAAA ATTAGCCGGG 601 TGTGGTGGTG CGCGCCTGTA ATTCCAGCAA CTCTGGAGGC TGAGAATCCT CGAGGCTAGC 661 GAGCTCAGGT ACCGGCCAGT TAGGCCAGAG AAATGTTCTG GCACCTGCAC TTGCACTGGG 721 GACAGCCTAT TTTGCTAGTT TGTTTTGTTT CGTTTTGTTT TGATGGAGAG CGTATGTTAG 781 TACTATCGAT TCACACAAAA AACCAACACA CAGATGTAAT GAAAAATAAA GATATTTTTA 841 ATGGCGGCCT GGTCCAATAC CTCCCGGTAC CTTTAATATT ACTTTACTTT ATCCTTGAAG 901 AGAACGGTAC CTAGTTAACC CCTGGATTAA ATTGCCTTTC AATTAATAAT TTGAAAAAAG 961 GACGAGGTAA TGAAGCATTC CCAACAATTT TTCGTGGTTC GCACTTCATT TCCCTTTCCT 1021 TTTGGCAGGC AATTTTTAGC ATTGGCCTTG GTTTATATTG CACCAACTCC CGAAAACGCT 1081 TGGGTGAAAA GTTCAGAAGA TGCTGAAGGA TTCAAACCTG GGTTGCAAGA AGGTTGGCTT 1141 ATTATCCGAA CTTGGAATTT CCCTCACAAA TAAAGCG //
Primer: pGL4-136R Sequence file: RDB07388_A7Eob.seq
>RDB07388_07388_A7Eo_pGL4-136R_D02_11_ABI24.ab1 1 GCACCTTGTT TTTGGCATCT TCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG 61 GCCGCCGAGG CCAGATCTTG ATCATGATCC CAGCCTCTGA GTCCTGAGAA CTTCTTGAGT 121 CCGGATGGGG ACTTTATAGT CGGGAGTCGT GGCGACGCTG ATTGGCTTCT CTAGAAACCC 181 GACACCCATT GGGAATGAGA ACTGAGTCTG CGTCATGAGT ATCCAGGAAG AAGGACACAT 241 GACCAGGTTA CGAGAGGAAC GGGAAACTGC AGAGTTTCCC AGCAATCAGC AGTTCTTAAA 301 CTTTTAGGTT TCGGTATCTC TGCACACTCT TAGAAATTAG TCCTGGCTGG GCGCGGTGGC 361 TCACGCCTGT AATCTCAGCA CTTTGGGAGG CCAAGGCGGG CTGATCACCT GAGGTTGGGA 421 GTTCGAGACC AGCCCGACCA ATATGGAGAA ACCCTGTCTC TACTAAAAGT CCAAAAAATT 481 AGCCGGCGTG GTGGCGCATG GCTGTAATCC TGGTACTTGG GAGGCTGAGA CAGGAGAATC 541 ACTTGAACCC AGGAGGAGGA GGTTGCGGTG AGCCAAGATC GTGCCATTGC CCTCCAGCCT 601 GGGCAACAAG AAAAAAGAAA TTAGTGAGGA CCCAAATAAA TTTTGTTTCT GTGGATTGTA 661 TCAATTTTTA TCATATATAA ATTAAAAGAT ATTAAAACAA AATATTAAAA ATATTAATTC 721 ATTTAAGAGA ATATTAATAA CCCATTACAT GTTAATAGAA ACAACTTTTT TTTTTTTTTT 781 GAGTCAGAGT CTTGCTCTGT CCCCAGGGCT GGAGTGCAGT GGCATGATCC TCGGCTCACT 841 GCAACCTTCC TTCTCCTGGG GTTCAGGCG //
Primer: pAxALNL_F1 Sequence file: RDB07388_A7Eoc.seq
>RDB07388_07388_A7Eo_pAxCALNL_F1_E02_14_ABI24.ab1 1 CTCAAGGTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA 61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC 121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT 181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT 241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC 301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG 361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG 421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG 481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA 541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC 601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT 661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA 721 GCGTTTGGCC ACGGCCTCAC CTACCTCCTT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC 781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG 841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC 901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT 961 GAACATGCCG AAGCCGTGGT GAAATGGCAC CACGCTGAGG ATAGCGGTGT CGGGGATGAT 1021 CTGGTTTGCC GAAGATGGGG GTCGCGGGCA TGACTGAAAT CGGGACACAG CGATGCGATG 1081 CGGTAGGCTA CGCCCTGGCC ATCAGTACTG CCACTACTGT TCATGAATCA GGGGCGGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

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Other resources in our bank

human major histocompatibility complex, class I, E

External Database
Human major histocompatibility complex, class I, E

Reference sequence