RDB07378 - pGL4-phSSTR2

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pGL4-phSSTR2 (#RDB07378)

Promoter collection, Human SSTR2 promoter

Alternative name	U2737(2115)-34
Clone info.	PCR amplified human SSTR2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 73163842 to 73165161(NC_000017.11); relatively -1168 to +152, where +1 corresponds to 1 nt of NM_001050.3.
Comment	PCR cloning, forward primer: GGAAACTCCTGATCCCCATC; reverse primer: CTCCTCGACCCTCTTGTGC. Entire sequence of promoter region has not been confirmed. Known differences (at least) : 10nt (GTGTGTGTGT) insertion between 73164123 to 73164124nt, and G to T substitution at 73164991nt on NC_000017.11.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human somatostatin receptor 2 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07378.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07378	pGL4-phSSTR2	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phSSTR2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07378).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07378_B2I2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (pr 665) Region: Pause site,insert 5' Sequence file: RDB07378_B2I2a.seq
>07378_07378_B2I2_3_pGL4ins_F_B01_02_ABI08.ab1 1 NNNNNNNANN NNNNNNNNNC NNANNCNAAA NAGGCTGTCC CCAGTGCAAG TGCAGGTGCC 61 AGAACATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA GGATGGAAAC 121 TCCTGATCCC CATCTCACAG TGGGTACCCC AAGATACAGC TTGGCCCAGA TAGAAGAGAG 181 AGCTGGCTGC TGCTGTACCT GGTGGTTGCT TTTCCATGTC ACTCATCTAT CTCTAAGGCT 241 CACCCCACCT GCCACACAGC TTCTGGACTT TTACTTTTCC CTGGTGAATT GGAAATCAGA 301 TAATAGGATG GACAAATCAA GGCAGCTCAG CTCACAGAGA AAGCTAGTTG GGGTTTGCTT 361 TCTGTTTTCA CATCCTCTAA AACTGTACTG AGGATGGTGT GTGTGTGTGT GTGTGTGTGT 421 GTGTGTGTGT GTGTGTGTGT ATACCTATGT ACTTGGAGGA GGGTGAGGAA GGGGAGGCTG 481 CANGATTGAT TATGACTTAT TAAACTATTG GAAAAGAGGC TGAATCATCA CAGTCAAAAA 541 TAAACAGCTG CAACTCCATG CGACTCCCAC TTCTTGCCTG TTCCCCTTTG CTCCCTCACA 601 GCATGTNNGA GAGACTGTGC TGGGTCAGAC CANCAGGTGC TGACTGACGT GGCTACAGCA 661 AGGGGACCAC CTGTATGGCC GCTGCACCTC CAAGCTGTGG GACGCANGGA CAGCTGGGAC 721 CAGTCNACGT CCACTGGCCC TCTGATGGCT CCTANNACTG AATCTTGGAC TCCAGGTGCG 781 GGTTTACACT CCCTGCGCTC ATTGNGAACN NNNNGGANNA GCGCTATCCC CTGAGCCCTT 841 TTTCTCCCTA CTCTNANNCT GGCCCNGCGC CCNGCGCCCG GGNGCTGNNC CACGGNNANC 901 ACAGCTTTNN CNNACTTNNT NNGNNAANNA ANNCACANNN NNCCCNNNNA NAANNCNGGG 961 GTCNCCCNNN NCGNNNCNNC NNCTGNCNNN NNNNTATGGG NNNGAANGNN NNNNNNNTNN 1021 NNNNNNNN //
Primer: pGL4ins_R (pr 666) Region: insert 3' Sequence file: RDB07378_B2I2b.seq
>07378_07378_B2I2_3_pGL4ins_R_C01_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ANGGCGCTGG GCCCTTCTTA ATGTTTTTGG 61 CATCTTCCAT GNNNNNNNTT TACCAACAGT ACCGGATTGC CAAGCTTGGC CGCCGAGGCC 121 AGATCTTGAT CTCCTCGACC CTCTTGTGCC GGCTCCCGCT AGCCCCTCGC ACGTAGAGAG 181 CTCCCCTCGC CCGCAGACCC CCGGCGCCAA GCCTCAGACT GGGGCTCTGG GCGCTGGTGG 241 TCTTGCGAGC GCGCGCGCAT GGGTGGCTGC GCCGCTGGGA CTGGCCCAGC CAGCCCGGGC 301 ACCTCGGCAC ATTTCCCCAC GCCCCCCTCC CGCTACGTCA CAGCGGCTTC GCTCCGCCCC 361 CCCAACCCCT CCTCCCGGGT CCTGCAGAAG GACAGGGGTG AGACCGGCTG AAACGCCTGC 421 GGGTGGCCGC AGGCAGAGCT TACAGACAGC CGGGGAGCGG GGAGAGAGGG GACCCGCCTG 481 CGGGGGCGTA AAAGGTTAAG CGTTTTCGGT CTCCTCAAGA CTCATGGCAA GGACAAGCGC 541 TATTTTCCCT CTGATTTCAC AGAGTCGATT CCCTTCCACC CATATATTCT AGGCAGCTGG 601 AGTGGCGGCC GGGGTGACCC AGACTTATAC CGGGACCTCT GTGATTTCCT TAGCCAAACA 661 AGTTAGCAAA GCTGTGTTTA CCGTGGGCCA GCCCCGGGCG CAGGGCGCAN GGCCAGGCTA 721 AGAGTAGGGA GAAAAAGGGC TCANGGGATA GCGCTTCTCC ATGCAGTTCC CAATGAGCGC 781 AGGGAGTGTN AACCCGCACC TGGNNTCCAA GANTCAGTCC TAGGNNCCAT CAGANGGCCA 841 GTGGANGTCN ACTGGTCCCA GCTGTCCCTG CGTCCCACNN NCTNNGNNNT GCANCGGCNN 901 TNCAGGTGGN CCCCTNGCTG TNNNNNNNGT NNNNNNANCA NNNGCTGGNN NGNCCCANCN 961 NAGTCNNTCC TACATGCNNN NAGGNANCAA NNNGGAACNN NNAAGAANNN NNNNNNN //
Primer: pGL4pA_R (pr 664) Region: SV40 pA,luc 3' Sequence file: RDB07378_B2I2c.seq
>07378_07378_B2I2_3_pGL4pA_R_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNANCGAN TTNNCNNCAT TTGTAGAGGT TTTACTTGCT 61 TTAAAAAACC TCCCNCACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT 121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC 181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA 241 TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT ACACGGCGAT 301 CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT CCAACTTGCC 361 GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT TCTTGGCGGT 421 TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT TACCGTGTTC 481 CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC CGGCGACCCC 541 GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG GGGCTACCTG 601 GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT GCTCGTCCTC 661 GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA GAGCGTTTGT 721 AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC ACAGCTCGCC 781 GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAN CCTCNAAGAA 841 NNGNCACCAC CTNGCCTACN GCGCCNNGNT TGTCGTCCCC TNNNGGGGTG ATCAGANTGG 901 CGCTNGTTGT TTCTGTCAGG NNGNANCCCN NGCNGNNGNC NTGGNNNNNG GANNCGTTNG 961 GCCANNNCCT CACNNANCNT CCNTNNNNAN NNGNCGCCCC NNCGCNNNNG ATNNNNNGCN 1021 ANNNTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

Resource search

Link

Other resources in our bank

human somatostatin receptor 2

External Database
Human somatostatin receptor 2

Reference sequence