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pGL4-phNFATC4

Promoter collection, Human NFATC4 promoter

Catalog number RDB07376
Resource name pGL4-phNFATC4
Alternative name U2725(2103)-5
Clone info. PCR amplified human NFATC4 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 24366849 to 24368163(NC_000014.9); relatively -62 to +1253, where +1 corresponds to 1 nt of NM_001136022.2; relatively -1335 to -20, where +1 corresponds to 1 nt of NM_004554.5.
Comment PCR cloning, forward primer: CCCGTAGAGCCTGTTTCTCA; reverse primer: CCCCCAAACTTCTTCAAAGC. Entire sequence of promoter region has not been confirmed. Known difference (at least): deletion of C at 24368132nt, deletion of 24367993nt, and deletion of CCT at 24367749 to 24367751nt on NC_000014.9.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4

          Reference sequence
            

          Distribution information

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          RDB07376 pGL4-phNFATC4 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07376.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07376_A1Djp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4ins_F
          Region: pause site, insert 5'
          Sequence file: RDB07376_A1Dja.seq check
          >07376_07376_B1EcF_pGL4ins_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT
             61 TTCTCTGGCC TAACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGATCCC GTAGAGCCTG
            121 TTTCTCATTA GAGTAACGGG CGCGGTCCCC GCCCGGCTTC TGAGTGTTTG TAAACGTCTG
            181 ACCTGGGGCC GTCGCTTAAC CGTTTAGTTG CTGGGATGGG GCGGCGTTGG GGGTGCGGCC
            241 CTGAACCGGA GGGATTTAGA GACTGGAGAC GCGGCCTCTA AGAGAGGTTG AAACTGTGTG
            301 TGTGTGGGAG AAAATGATAA CCACCCTCCC ATCTCTCCTA CCCGCCAGCC TCGCCAGTAT
            361 CTCCCACCGA GTCACGAATC TCCCATCTAA CTCCCTCTCA CACAACCCAG GCCTCTCCAA
            421 GCCTGACTTT CCCGGAAACT CCAGTCCAGG TCTTCCTTCC TCCTCCAGCC CAGGTGACAG
            481 GTGTCCAGCC TGTTGGGGGC GGGGGGGCCA AGGAGGGGGA AGAGGAGGGG AACCCACAGG
            541 GTCCGGANGT TTCAGGCCCC TAGTAAACCT GGCAAGGANT TTTAGAAGAG CCGGANTGGG
            601 ATGCTATCGG GTCAGANATT CATTCCNGAN TACNGTTCTA GGGANGANGC TGNCNACTTC
            661 CCGNGGANNN TAATTCNNNG NCCNNCTGGG NNGNTGGGNN NNNGNCANCN GNNAANCNNG
          721 NNNNNNNNNN
          //
          Primer: pGL4ins_R
          Region: luc, insert 3'
          Sequence file: RDB07376_A1Djb.seq check
          >07376_07376_B1EcF_pGL4ins_R_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG CGCTGGGCCC TNNNNNNTGT TTTTGGCATC
             61 TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT TGGCCGCCGA GGCCAGATCT
            121 TGATCCCCCA AACTTCTTCA AAGCGGTCCC CCCCCAGCCT GTCCCTGATT GGCTGCCGGG
            181 GATGCTCCAG CCCCTCCTCC AGGGATGAGA TGGGAAAACC ACGCGCCCCT CGTCCTCCCC
            241 CACCCTCCCT CCCTCTGTCC TCTCTTCAGT CCCTCCCCCT CAAAAAAAAA AAACTGAATT
            301 GGAAAACGGT CCTTCAAGAG TGATACTAAT GTTGCCTATT GGTCCGCCGT TGTGACAATC
            361 ATTTCCCGCA GAGAGCCCAG CCCCCACCTC CCCTAGTCTC AGTTCAAGGT TCTGGGTCCG
            421 AGAGCCTTAA AGGGGCCACA TCCTGGGCCC CCAGGGTAGA GAGCCCACGG AAGACCCTTT
            481 TTTAAATTCT GAAGACTGTG TAGATAGCAC CTAGCAAGGC AGAACCACTT CTCCCCTTTC
            541 CCTCCAAGCC TGAGGCCAGG AGGGTCCTGG GGGCAGAGTG GCACCGAGTT GCTCGGAAGA
            601 NGAAAAGGCT ATTCTTCCGC CTCCCTTGNC NGGGGCTACT CTGCCCGGGC CTTCNGGAAN
            661 TCTTTGCGCT GNCNNANCAG CNNNNCNTCN NCCNTTNNTN NNNNNNCCCN NANGNNGNNN
          721 NNNNNNTNNN NNNNNNN
          //
          Primer: pGL4pA_R
          Region: SV40 polyA, luc
          Sequence file: RDB07376_A1Djc.seq check
          >07376_07376_B1EcF_pGL4pA_R_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCNNCA TTTGTAGANN GTTTTACTTG
             61 CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG
            121 TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
            181 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
            241 TATCTTATCA TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA TTACACGGCG
            301 ATCTTGCCGC CCTTCTTGGC CTTAATGAGA ATCTCGCGGA TCTTGCGGGC GTCCAACTTG
            361 CCGGTCAGTC CTTTAGGCAC CTCGTCCACG AACACAACAC CACCGCGCAG CTTCTTGGCG
            421 GTTGTAACCT GGCTGGCCAC ATAGTCCACG ATCTCCTTCT CGGTCATGGT TTTACCGTGT
            481 TCCAGCACGA CGACTGCGGC GGGCAGCTCG CCGGCATCGT CGNCGGGCAG GCCGGCGACC
          541 CCGGCGTCGA ANANGTTGGG GTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl