Resource data sheet
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pGL4-phEZH2 (#RDB07370)

Promoter collection, Human EZH2 promoter

Alternative name U2716(2094)-1
Clone info. PCR amplified human EZH2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 148885505 to 148884301(NC_000007.14); relatively -1156 to +49, where +1 corresponds to 1 nt of NM_004456.4.
Comment PCR cloning, forward primer: CGCCTTGCAAATATCCTCAC; reverse primer: CAATCGCCATCGCTTTTATT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human enhancer of zeste homolog 2 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07370.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07370 pGL4-phEZH2 Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pGL4-phEZH2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07370).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles