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pGL4-phPCNA (#RDB07365)

Promoter collection, Human PCNA promoter

Alternative name U2686(2064)-4
Clone info. PCR amplified human PCNA promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 5127783 to 5126581(NC_000020.11); relatively -1161 to +42, where +1 corresponds to 1 nt of NM_002592.2.
Comment PCR cloning, forward primer: TGGCCTCCTTCCTACTCTCC; reverse primer: AACCGGTTACCTCCAGAACC. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name Human proliferating cell nuclear antigen genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07365.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

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付加的使用条件：
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RDB07365 pGL4-phPCNA Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPCNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07365).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

user_report Lin, Y.C., Di-n-butyl phthalate promotes the neural differentiation of mouse embryonic stem cells through neurogenic differentiation 1. Environ. Pollut. 347: 123722 (2024). PMID 38460589. [PubMed] [Article] [RRC of NBRP]
user_report Huang, L., Interferon regulatory factor 7 protects against vascular smooth muscle cell proliferation and neointima formation. J. Am. Heart Assoc. 3 (5). pii: e001309 (2014). PMID 25304854. [PubMed] [Article] [RRC of NBRP]