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pGL4-phXPC (#RDB07351)

Promoter collection, Human XPC promoter

Alternative name U2751(2129)-1
Clone info. PCR amplified human XPC promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 14179875 to 14178575(NC_000003.12); relatively -1203 to +98, where +1 corresponds to 1 nt of NM_004628.4.
Comment PCR cloning, forward primer: ATGCACATGAAAAAGCCCTACT; reverse primer: TGTCTGGGCAAATTCCACTT. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human xeroderma pigmentosum, complementation group C genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07351.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07351 pGL4-phXPC DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phXPC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07351).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07351_A4Kl.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07351_A4Kla.seq check
>07351_07351_A4Kl_2_pGL4-4174F_D01_10.ab1
    1 GTTGGGGTCC AGGAAATTTC TCTGGCCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
   61 GGATATGCAC ATGAAAAAGC CCTACTGCTA CCTAAGCCTC TGTCTGGGAT GCCCACTGTC
  121 TGCTCTTTTC ATCCTGTAGC TTTAAATATT GGCTTCTCAA AGAGGCTTCC TCTGATCATC
  181 TAACTCATTC ACTCAAATAT TTTTTGAGTG TTTCCAGCAC TGTTCTAAAC CCCAAAGACA
  241 AAAGAATGAA CAAAACAAAA TCCCTGCTCT TATGGCATTT ACATTCTAGT GGGGAGAGAT
  301 TCACATTAAA CAAAGATGCA TACATTTAAT ATGAAAAAAT AACTCTCTAT GAAGAAAAAT
  361 GAGGCAGGGT AACCAGAGTA GCGGGAGTGC TATTTGGGCA GAAGTCCTCT CTGAGGAGGT
  421 CAGATTTCAG TAGAAAGTTT AAGTTGTGGC ATGATCTCCA AAGATATTTG AGGAAGAAAA
  481 AGACCAGGTG AAAAAGCTTC TAGGACCATC TGTTATTCTG CATACCAGTT CCTTGTTTCC
  541 TTCACAGCAC ATCACAGTTC CTGGAAGTCT TACATATTTA TATTTACTTT TATCTGTTCT
  601 CCTCGCAAAT ATAAGCTCCA AGTAATGGCA AGGATCTCTT ATATGTCGAT GTCAGTGTCC
  661 CTGGCCCTTA GAACAGTGCC TAACCATACA AAGCTGATGT TCACTCTATC TGCTGAATGA
  721 ATAAATAATT GTTGCGCTCA CTAATGATAA CAACTCCTAA CTTTATTACC TGGCTTTCTA
  781 TTGGAATCCT GACAGCTCCA TGAGGTAGCT AAGGATAATA ATCTACAGAT TTAAAAAAAT
  841 GAAGCGAAGA GTGTGAGAAT AGTCATCAGG TGGGCTTCTA ACAGGAAAAG GTCTCAGGGC
  901 CTACGGCAAA ATTCGGAGGG GGGGTTACGG AAAGGACAGG GTGGATTAGG CCATGGTGAC
  961 TTGGAGAGAG GAGGCACGAC TGGCCATTTT TTCCTGAGTC TGGAAAAAAG CAGCCTAGTA
 1021 CAAGAAAGCT TCCTTCGAGC GACCTCCTTT AAAAATACAC TTTACCCTTG GGTCTACGTC
 1081 GTCGTCCATG ATTTCCAACC CTGCGTCCAT TCCCGGACTC TCGCGAAGAC ACAAGAAGCC
1141 AAACCGTCTC AAATC
//
Primer: pGL4-136R
Sequence file: RDB07351_A4Klb.seq check
>07351_07351_A4Kl_2_pGL4-136R_G01_19.ab1
    1 GGTTTCTAGG TTTTTTGGCA TCTTCCATGG TGGCTTTACC AACAGTACCG CGATTGCCAA
   61 GCTTGGCCGC CGAGGCCAGA TCTTGATTGT CTGGGCAAAT TCCACTTGGC GAGTGACGCA
  121 CCCGGCCGCG ATGCGCTAGA ACGCCGGCCC CGCCCCGAGG CGGTGCGCTT GGCCACGCCC
  181 CTTCGTTGGA GGCCTAGTCA CGCCCCTAAA GGAAGCTCCA CCCCATACCC GTGGCCACTA
  241 AAAAACTGCG GGAAGCAGGA AAGAGCACAA CAGGTTTCTC TCTATTTCCG GTGTATTGTA
  301 TCCTCACGTT TCCGGAGATT GACGTTGCTC TTGTGTTCTC GCGAGAGGCG GGAAAGGGCG
  361 CAGGGTTTGA AACATGGCGG ACGACGTAGA CCAGGTAAGT GTATTTTAAG GAGGTCGCTC
  421 GAAGGAGCTT CTTGTACTAG GCTGCTTTTT CCAGACTCAG GAAAAATGGC CAGTCGTGCC
  481 TCCTCTCTCC AAGTCACCAT GGCCTAATCC ACCCTGTCCT TTCCGTAACC CCCCCTCCGA
  541 ATTTTGCCGT AGGCCCTGAG ACCTTTTCCT GTTAGAAGCC CACCTGATGA CTATTCTCAC
  601 ACTCTTCGCT TCATTTTTTT AAATCTGTAG ATTATTATCC TTAGCTACCT CATGGAGCTG
  661 TCAGGATTCC AATAGAAAGC CAGGTAATAA AGTTAGGAGT TGTTATCATT AGTGAGCGCA
  721 ACAATTATTT ATTCATTCAG CAGATAGAGT GAACATCAGC TTTGTATGGT TAGGCACTGT
  781 TCTAAGGGCC AGGGACACTG ACATCGACAT ATAGAGATCC TTGCCATTAC TTGAGCTTAT
  841 ATTTGCGAGG AGAACAGAAT AAAAGTAAAT ATAAATATGT AAGACTTTCC AGGAACTGGT
  901 GATGTGCTGT GAGGAAACAA GGACTGGTAT GCAGAATAAC AGAATGGTTC TTAGAGCTTT
  961 CACCTTGTCT TTTTTCTTCC TCAAATTATC TTTGGGAAGA TCATGCACAA CCTTAACCTT
 1021 TCTACTGGAA TCTGAACCTT CTCAGAGAGG AACTTCTTGC CAATAAGCAC TCTCGGCTAC
1081 CTTGTACCTG CTTCAATTCT CTAGAGTATT TCATTAAAGT GATGGCCACT TTGGTTAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles