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pGL4-phCX3CL1 (#RDB07340)

Promoter collection, Human CX3CL1 promoter

Alternative name U2710(2088)-8
Clone info. PCR amplified human CX3CL1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 57371363 to 57372598(NC_000016.10); relatively -1127 to +109, where +1 corresponds to 1 nt of NM_002996.6.
Comment PCR cloning, forward primer: TGGAGACACCAGTACAGCTCA; reverse primer: GAGCAGCCACGACAGAGATA. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human chemokine (C-X3-C motif) ligand 1 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07340.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07340 pGL4-phCX3CL1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCX3CL1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07340).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07340_A4Df.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07340_A4Dfa.seq check
>07340_07340_A4Df_1_pGL4-4174F_C02_08.ab1
    1 GATGGGGCAG AACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
   61 ATTGGAGACA CCAGTACAGC TCAGTCCTAT ACCAAGGCAG AGCTGCTGAC TCTTCTGGAG
  121 TCTCAGGGAT TGTTGCCACT GCCTTTAGGG AAGGCTGCCA TGAAGGCAGA CTGTGTTCTA
  181 ATGTGCTCCC AAGGCTGGCA AGCCTCTGCA CCCTTCGGTG AGAGCCTGTT GGAGGGCCGG
  241 TGCTGGGAAG CCCTCTCCCC ATTGCACTAG CCCAATGCTC TCTTTGGGCA GATAGAGAAC
  301 TCAGGCCCCA CGAGAGGCAA GCCTTATCCA AGCCACGGCT ACAGAGTTGA GGAGCCAAGA
  361 GCCACAGAGT TATCGGGGAT GGGTTGGCAG CCACCTTGTT CTTTGGGCTA ACAGGGGCCA
  421 GAGCAGAGGT GGGAGTGGGT GCAAAGCAGG GAGCGGGAGA CCGGGAGGTG GAGGCAGGGA
  481 GGCCAGGGCT CTCTCCTGGG TCCTGAGCTG TCCACATTCT GCTCATCATG GTGGTGGGAG
  541 GGGAGTTCTC CACTCTGATG CTGTGGATAA CCTGGGGTGG CTCCGGGCCT CAGTTTCCCC
  601 ATCTATCCAT ATGAAGTTGG ACTCAATCCT TCTTTCAGCA GACCCTGCAC CAAGCCCCTG
  661 CTGACTTCAG GCTCCCTGCT GGGCTCTGGA GATGCATAGA TCAACAAGGT CCCCCAGGAG
  721 CTTGGGATTC TAAGAGGGGA AATTTAGGGG TCCATCCTGA TGTGGGCGAG GCATTCTCTG
  781 GATCAAAGAA GAGCTTGTCA GACCCAGGAT GGAGGGGCCC TGAGTCTCTC CCGGCCCCTT
  841 GGCGCCCCGC TCTCTATTCC ACGGCCTGCT GGCCTTTTGT GTGTTGCCCA CTTAGGATCA
  901 AGGGCCCCAG GCCAGATCCT AGGGCATGTT CCCAGCTTGT GGCAGGAGAG GAGGCCTTTG
  961 TACCCAGGCA GGCCCAGTCC AGCCTCCCGG GGAACGTCCC AGTATGACAA GCCTAGGATG
 1021 GTGAGAAGCC CAGGGACACA AAGCCCGGCT TGGATCCTTG GCAACATCCC TGAGGAATCC
 1081 AGCCGGCCTG CACACTGGCT CCATCTCTTC CCTTCACCTC CTGGCTGCTG AGCCTGAGCC
1141 GAGGGCAGGC CGAACCCCAT TATTA
//
Primer: pGL4-136R
Sequence file: RDB07340_A4Dfb.seq check
>07340_07340_A4Df_1_pGL4-136R_D02_11.ab1
    1 GTTTTTAATG GTTTTGGGCA TCTTCCATGG TGGCTTTACC AACAGTACCG GATTGCCAAG
   61 CTTGGCCGCC GAGGCCAGAT CTTGATGAGC AGCCACGACA GAGATATCGG AGCCATGGCT
  121 GAGGGTGGGC AAGAGTCCCG GCGGGGGCCA GGCAGGCGGC TAGAGCCAGG CGGCAGAGCT
  181 CAGTGCTGTC CCCTTGCCGC CGCCAGAGAT CTGTGGCTTT TTATAATGGG GTTGGCTGCC
  241 TCCGCCTCCA GCCCCCGCAG GCAGGAGGGT GGGAGGGGAG AGGAAGGGAG CCAGTGTGCA
  301 GCCGCTGGAT TCCTCAGGAT GTTGCCAAGG AACCAAGCCG GCTTTGTGTC CCTGGGCTTC
  361 TCACCATCCT AGGCTTGTCA TACTGGGACC TTCCCCGGGA GGCTGGACTG GGCCTGCCTG
  421 GGTACAAAGG CCTCCTCTCC TGCCACAAGC TGGGAACATG CCCTAGGATC TGGCCTGGGG
  481 CCCTTGATCC TAAGTGGGCA ACACACAAAA GGCCAGCAGG CCGTGGAATA GAGAGCGGGG
  541 CGCCAAGGGG CCGGGAGAGA CTCAGGGCCC CTCCATCCTG GGTCTGACAA GCTCTTCTTT
  601 GATCCAGAGA ATGCCTCGCC CACATCAGGA TGGACCCCTA AATTTCCCCT CTTAGAATCC
  661 CAAGCTCCTG GGGGACCTTG TTGATCTATG CATCTCCAGA GCCCAGCAGG GAGCCTGAAG
  721 TCAGCAGGGG CTTGGTGCAG GGTCTGCTGA AAGAAGGATT GAGTCCAACT TCATATGGAT
  781 AGATGGGGAA ACTGAGGCCC GGAGCCACCC CAGGTTATCC ACAGCATCAG AGTGGAGAAC
  841 TCCCCTCCCA CCACCATGAT GAGCAGAATG TGGACAGCTC AGGACCCAGG AGAGAGCCCT
  901 GGCCTCCCTG CCTCCACCTC CCGGTCTCCC GCTCCCTGCT TTGCACCCAC TCCCACCTCT
  961 GCTCTGGCCC CTGTTAGCCC AAAGAACAAG GTGGCTGCCA ACCCATCCCC GATACTCTGT
 1021 GGCTCTTGCT CCTCAACTCT GTAGCCGTGG CTTGGATAAG CTTGCCTCTC GTGGGGCCCT
 1081 GAGTTCTCTA TCTGCCCAAG AGAGCATGGG GCTAGTGCAA TGGGAGAGGC ATCCCAGCAC
1141 CGGCTCCTCC TAACCGAGCC TCTCTCCACC CGGAAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles