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pGL4-phMYC (#RDB07325)

Promoter collection, Human MYC promoter

Alternative name U2683(2061)-4
Clone info. PCR amplified human MYC promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 127734926 to 127736192(NC_000008.11); relatively -1305 to -38, where +1 corresponds to 1 nt of NM_002467.6.
Comment PCR cloning, forward primer: CACAAGGGTCTCTGCTGACTC; reverse primer: ATCCCTCCCTCCGTTCTTT. iPS Yamanaka factor. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human v-myc myelocytomatosis viral oncogene homolog genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07325.pdf

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RDB07325 pGL4-phMYC DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phMYC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07325).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07325_A9Gvp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F ()
Region: pause site,insert5'
Sequence file: RDB07325_A9Gva.seq check
>07325_07325_A9Gv_3_pGL4ins_F_E10_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNGN NAAATAGGCT GTCCCCNGTG CAAGTGCAGG TGCCAGAACA
   61 TTTCTCTGGC CTAACTGGCC GGTACCTGAG CTCGCTAGCC TCGAGGATCA CAAGGGTCTC
  121 TGCTGACTCC CCCGGCTCGG TCCACAAGCT CTCCACTTGC CCCTTTTAGG AAGTCCGGTC
  181 CCGCGGTTCG GGTACCCCCT GCCCCTCCCA TATTCTCCCG TCTAGCACCT TTGATTTCTC
  241 CCAAACCCGG CAGCCCGAGA CTGTTGCAAA CCGGCGCCAC AGGGCGCAAA GGGGATTTGT
  301 CTCTTCTGAA ACCTGGCTGA GAAATTGGGA ACTCCGTGTG GGAGGCGTGG GGGTGGGACG
  361 GTGGGGTACA GACTGGCAGA GAGCAGGCAA CCTCCCTCTC GCCCTAGCCC AGCTCTGGAA
  421 CAGGCAGACA CATCTCAGGG CTAAACAGAC GCCTCCCGCA CGGGGCCCCA CGGAAGCCTG
  481 AGCAGGCGGG GCAGGAGGGG CGGTATCTGC TGCTTTGGCA GCAAATTGGG GGACTCAGTC
  541 TGGGTGGAAG GTATCCAATC CAGATAGCTG TGCATACATA ATGCATAATA CATGACTCCC
  601 CCCAACAAAT GCAATGGGAG TTTATTCATA ACGCGCTCTC CAAGTATACG TGGCAATGCG
  661 TTGCTGGGTT ATTTTAATCA TTCTAGGCAT CGTTTTCCTC CTTATGCCTC TATCATTCCT
  721 CCCTATCTAC ACTAACATCC CACGCTCTGA ACGCGCGCCC ATTAATACCC TTCTTTCCTC
  781 CACTCTCCCT NNNNNTCTTG ATCAAAGCGC GGCCCNTNNC NNAGCCTTAN CGAGGCGCCC
841 TGCAGNNNGG NACGCGCGNG GCGTGGNGGN NGGCGCG
//
Primer: pGL4ins_R ()
Region: Luc5',insert3'
Sequence file: RDB07325_A9Gvb.seq check
>07325_07325_A9Gv_3_pGL4ins_R_F10_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNANNNNGNC GCTNGGCCCT TCTTANTGTT TTTGGCATCT
   61 TCNNTGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT
  121 GATATCCCTC CCTCCGTTCT TTTTCCCGCC AAGCCTCTGA GAAGCCCTGC CCTTCTCGAG
  181 GCAGGAGGGG AGCCAGGGAC GGCCGGGGCC CGGCGGTGGC GGCCGCGAGC AGCACAGCTC
  241 GGGGGTCCTC AGCCGTCCAG ACCCTCGCAT TATAAAGGGC CGGTGGGCGG AGATTAGCGA
  301 GAGAGGATCT TTTTTCTTTT CCCCCACGCC CTCTGCTTTG GGAACCCGGG AGGGGCGCTT
  361 ATGGGGAGGG TGGGGAGGGT GGGGAAGGTG GGGAGGAGAC TCAGCCGGGC AGCCGAGCAC
421 TCTAGCTCTA GGATGTAAAC AGAGTAAGAG AGCCGCATGA ATTAACTACG NGCGNCT
//
Primer: pGL4pA_R ()
Region: SV40p(A),Luc3'
Sequence file: RDB07325_A9Gvc.seq check
>07325_07325_A9Gv_3_pGL4pA_R_A08_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTTATCGN NTTTNNNNNA TTNGNNNAGG TTTTACTTGC
   61 TTTAAAAAAC CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT
  121 TGTTAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
  181 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
  241 ATCTTATCAT GTCTGCTCGA AGCGGCCGGC CGCCCCGACT CTAGAATTAT TACACGGCGA
  301 TCTTGCCGCC CTTCTTGGCC TTAATGAGAA TCTCGCGGAT CTTGCGGGCG TCCAACTTGC
  361 CGGTCAGTCC TTTAGGCACC TCGTCCACGA ACACAACACC ACCGCGCAGC TTCTTGGCGG
  421 TTGTAACCTG GCTGGCCACA TAGTCCACGA TCTCCTTCTC GGTCATGGTT TTACCGTGTT
  481 CCAGCACGAC GACTGCGGCG GGCAGCTCGC CGGCATCGTC GTCGGGCAGG CCGGCGACCC
  541 CGGCGTCGAA GATGTTGGGG TGTTGCAGCA GGATGCTCTC CAGTTCGGCT GGGGCTACCT
  601 GGTAGCCCTT GTATTTGATC AGGCTCTTCA GCCGGTCCAC GATGAAGAAG TGCTCGTCCT
  661 CGTCCCAGTA GGCGATGTNN NNGCTGTGCA GCCAGCCGTC CTTGTCGATG AGAGCGTTTG
  721 TAGCCTCGGG GTTGTTAACG TAGCCGCTCA TGATCATGGG GCCACGGACG CACAGCTCGC
  781 CGCGCTGGTT CACACCCAGT GTCTTANCGG TGTCCAAGTC CACCACCTTA GCCTCGAANA
  841 NNGGCACCAC CTTGCCTACN GCGCCANGCT TGTCGNCCCC TTCNGGGGGT GATCAGANNG
901 NCGCTGGTTG TTTCTGTCAN GNCNTANNCN TGNCGGANNC CNGGTAGGNG GA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles