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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phIL8

Promoter collection, Human IL8 promoter

Catalog number RDB07322
Resource name pGL4-phIL8
Alternative name U2680(2058)-6
Clone info. PCR amplified human IL8 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 73739341 to 73740603(NC_000004.12); relatively -1165 to +98, where +1 corresponds to 1 nt of NM_000584.3.
Comment PCR cloning, forward primer: ACCCTTCCTTGGTGCTCTTT; reverse primer: GAAGCTTGTGTGCTCTGCTG. Entire sequence of promoter region has not been confirmed. Known difference (at least): A to T substitution at 73,740,307 of NC_000004.12.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human interleukin 8 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human interleukin 8

          Reference sequence
            

          Distribution information

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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07322 pGL4-phIL8 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07322.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07322_A3I5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07322_A3I5a.seq check
          >07322_07322_A3I5_pGL4-4174F_E04_13.ab1
              1 ACTAGGGCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA
             61 TACCCTTCCT TGGTGCTCTT TATCTACAGA ATCCAAGACC TTTCAAGAAA GGTCTTGGAT
            121 TCTTTTCTTC AGGACACTAG GACATAAAGC CACCTTTTTA TGATTTGTTG AAATTTCTCA
            181 CTCCATCCCT TTTGCTAGTG ATCATGGGTC CTCAGAGGTC AGACTTGGTG TCCTTGGATA
            241 AAGAGCATGA AGCAACAGTG GCTGAACCAG AGTTGGAACC CAGATGCTCT TTCCACTAAG
            301 CATACAACTT TCCATTAGAT AACACCTCCC TCCCACCCCA ACCAAGCAGC TCCAGTGCAC
            361 CACTTTCTGG AGCATAAACA TACCTTAACT TTACAACTTG AGTGGCCTTG AATACTGTTC
            421 CTATCTGGAA TGTGCTGTTC TCTTTCATCT TCCTCTATTG AAGCCCTCCT ATTCCTCAAT
            481 GCCTTGCTCC AACTGCCTTT GGAAGATTCT GCTCTTATGC CTCCACTGGA ATTAATGTCT
            541 TAGTACCACT TGTCTATTCT GCTATATAGT CAGTCCTTAC ATTGCTTTCT TCTTCTGATA
            601 GACCAAACTC TTTAAGGACA AGTACCTAGT CTTATCTATT TCTAGATCCC CCACATTACT
            661 CAGAAAGTTA CTCCATAAAT GTTTGTGGAA CTGATTTCTA TGTGAAGCAC ATGTGCCCCT
            721 TCACTCTGTT AACATGCATT AGAAAACTAA ATCTTTTGAA AAGTTGTAGT ATGCCCCCTA
            781 AGAGCAGTAA CAGTTCCTAG AAACTCTCTA AAATGCTTAG AAAAAGATTT ATTTTAAATT
            841 ACCTCCCCAA TAAAATGATT GGCTGGCTTA TCTTCACCAT CATGATAGCA TCTGTAATTA
            901 ACTGAAAAAA AAATAATTAT GCCATTAAAA GAAAATCATC CATGATCTTG TTCTAACACC
            961 TGCCACTCTA GTACTATATC TGTCACATGG TACTATGATA AGTTATCTAG AAATAAAAAA
           1021 AGCATACATT TGATAATTCC ACCAAATGTG CAGCTTCAGT ATTTTTAAAT GTATATTAAA
           1081 ATTAATTATT TTTAAAGATC CAAGGAACTT CGTTCATACC TCCGTATTTG ATACGACCAA
          1141 TAGCCAGTTG ATGCACTCAG CATATGACCC CCCTA
          //
          Primer: pGL4-136R
          Sequence file: RDB07322_A3I5b.seq check
          >07322_07322_A3I5_pGL4-136R_F04_16.ab1
              1 GCATTATTGT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
             61 GGCCGCCGAG GCCAGATCTT GATGAAGCTT GTGTGCTCTG CTGTCTCTGA AAGTTTGTGC
            121 CTTATGGAGT GCTCCGGTGG CTTTTTATAT CATCACCCTA CTAGAGAACT TATGCACCCT
            181 CATCTTTTCA TTATGTCAGA GGAAATTCCA CGATTTGCAA CTGATGGCCC ATCCCCTCAG
            241 GGCAAACCTG AGTCATCACA CTTCCTATTT GTTCCTTATC AAATACGGAG TATGACGAAA
            301 GTTTTCTTTG ATCTTTAAAA TAATTTAATT TTAATATACA TTTAAAATAC TGAAGCTCCA
            361 CAATTTGGTG AATTATCAAA TGTATGCTTT TTTATTTCTA GATAACTTTA TCATAGTACC
            421 ATGTGACAGA TATAGTACTA GAGTGGCAGG TGTTAGAACA AGATCATGGA TGATTTTCTT
            481 TTAATGGCAT AATTATTTTT TTTCAGTTAA TTACAGATGC TATCATGATG GTGAAGATAA
            541 GCCAGCCAAT CATTTTATTG GGGAGGTAAT TTAAAATAAA TCTTTTTCTA AGCATTTTAG
            601 AGAGTTTCTA GGAACTGTTA CTGCTCTTAG GGGGCATACT ACAACTTTTC AAAAGATTTA
            661 GTTTTCTAAT GCATGTTAAC AGAGTGAAGG GGCACATGTG CTTCACATAG AAATCAGTTC
            721 CACAAACATT TATGGAGTAA CTTTCTGAGT AATGTGGGGG ATCTAGAAAT AGATAAGACT
            781 AGGTACTTGT CCTTAAAGAG TTTGGTCTAT CAGAAGAAGA AAGCAATGTA AGGACTGACT
            841 ATATAGCAGA ATAGACAAGT GGTACTAAGA CATTAATTCC AGTGGAGGCA TAAGAGCAGA
            901 ATCTTCCAAA GGCAGTTGGA GCAAGGCATT GAGGAATAGG AGGGCTTCAA TAGAGGAAGA
            961 TGAAAGAGAA CAGCACATTC CAGATAGGAA CAGTATTCAA GGCCACTCAA GTTTGTAAAG
           1021 TTAAGTATGT TTATGCTCCA GAAAGTGGTG CACTGCAGCT GCTTGGTTGG GGGTGGGAGG
           1081 GAGTGTATCT AATGCAAAGT TGTTATGCCT AGTGGCAAAG AGCCATCTGG GATCCACTCT
          1141 GATCAGCCCA CTGTTGCTCA TGCTCTTTAA TACCAAAAGA
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB07322_A3I5c.seq check
          >07322_07322_A3I5_pAxCALNL_F1_G11_20_ABI24.ab1
              1 ATAAGGCTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA
             61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC
            121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT
            181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT
            241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC
            301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG
            361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG
            421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG
            481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA
            541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC
            601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT
            661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA
            721 GCGTTTGGCC ACGGCCTCAC CTACCTCATT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC
            781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG
            841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
            901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT
            961 GAACATGCCG AAAGCCGTGG TGAAATGGCA CCACGCTGAG GATAGCGGTG TCGGGGATGA
           1021 TCTGGGTTGC CGAAGATGGG GTCGCGGGCA TGACTGATCG GACACAAGCG GGTGCGTTGC
           1081 GGTAGGCTAC GCCCATTGGG GCCAATTCGT ACTGGCACTT ACTGTTCAAT GATCAAGGGC
          1141 GAATGGTTTT TTGTGTTCCC CGGGGTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl