Promoter collection, Human IL8 promoter
Alternative name | U2680(2058)-6 |
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Clone info. | PCR amplified human IL8 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 73739341 to 73740603(NC_000004.12); relatively -1165 to +98, where +1 corresponds to 1 nt of NM_000584.3. |
Comment | PCR cloning, forward primer: ACCCTTCCTTGGTGCTCTTT; reverse primer: GAAGCTTGTGTGCTCTGCTG. Entire sequence of promoter region has not been confirmed. Known difference (at least): A to T substitution at 73,740,307 of NC_000004.12. |
Vector backbone | pGL4.10 [Luc2] (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Human interleukin 8 genomic DNA |
Depositor|Developer | DNA Bank, | |
Remarks, protocol and/or map (pdf) | RDB07322.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 謝辞の表明を必要とする。 MTAに書く付加的使用条件: 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB07322 | pGL4-phIL8 | DNA solution |
Materials & Methods section:
The pGL4-phIL8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07322). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB07322_A3I5p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pGL4-4174F Sequence file: RDB07322_A3I5a.seq |
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>07322_07322_A3I5_pGL4-4174F_E04_13.ab1 1 ACTAGGGCAG ACATTTCTCT GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA 61 TACCCTTCCT TGGTGCTCTT TATCTACAGA ATCCAAGACC TTTCAAGAAA GGTCTTGGAT 121 TCTTTTCTTC AGGACACTAG GACATAAAGC CACCTTTTTA TGATTTGTTG AAATTTCTCA 181 CTCCATCCCT TTTGCTAGTG ATCATGGGTC CTCAGAGGTC AGACTTGGTG TCCTTGGATA 241 AAGAGCATGA AGCAACAGTG GCTGAACCAG AGTTGGAACC CAGATGCTCT TTCCACTAAG 301 CATACAACTT TCCATTAGAT AACACCTCCC TCCCACCCCA ACCAAGCAGC TCCAGTGCAC 361 CACTTTCTGG AGCATAAACA TACCTTAACT TTACAACTTG AGTGGCCTTG AATACTGTTC 421 CTATCTGGAA TGTGCTGTTC TCTTTCATCT TCCTCTATTG AAGCCCTCCT ATTCCTCAAT 481 GCCTTGCTCC AACTGCCTTT GGAAGATTCT GCTCTTATGC CTCCACTGGA ATTAATGTCT 541 TAGTACCACT TGTCTATTCT GCTATATAGT CAGTCCTTAC ATTGCTTTCT TCTTCTGATA 601 GACCAAACTC TTTAAGGACA AGTACCTAGT CTTATCTATT TCTAGATCCC CCACATTACT 661 CAGAAAGTTA CTCCATAAAT GTTTGTGGAA CTGATTTCTA TGTGAAGCAC ATGTGCCCCT 721 TCACTCTGTT AACATGCATT AGAAAACTAA ATCTTTTGAA AAGTTGTAGT ATGCCCCCTA 781 AGAGCAGTAA CAGTTCCTAG AAACTCTCTA AAATGCTTAG AAAAAGATTT ATTTTAAATT 841 ACCTCCCCAA TAAAATGATT GGCTGGCTTA TCTTCACCAT CATGATAGCA TCTGTAATTA 901 ACTGAAAAAA AAATAATTAT GCCATTAAAA GAAAATCATC CATGATCTTG TTCTAACACC 961 TGCCACTCTA GTACTATATC TGTCACATGG TACTATGATA AGTTATCTAG AAATAAAAAA 1021 AGCATACATT TGATAATTCC ACCAAATGTG CAGCTTCAGT ATTTTTAAAT GTATATTAAA 1081 ATTAATTATT TTTAAAGATC CAAGGAACTT CGTTCATACC TCCGTATTTG ATACGACCAA 1141 TAGCCAGTTG ATGCACTCAG CATATGACCC CCCTA // |
Primer: pGL4-136R Sequence file: RDB07322_A3I5b.seq |
>07322_07322_A3I5_pGL4-136R_F04_16.ab1 1 GCATTATTGT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT 61 GGCCGCCGAG GCCAGATCTT GATGAAGCTT GTGTGCTCTG CTGTCTCTGA AAGTTTGTGC 121 CTTATGGAGT GCTCCGGTGG CTTTTTATAT CATCACCCTA CTAGAGAACT TATGCACCCT 181 CATCTTTTCA TTATGTCAGA GGAAATTCCA CGATTTGCAA CTGATGGCCC ATCCCCTCAG 241 GGCAAACCTG AGTCATCACA CTTCCTATTT GTTCCTTATC AAATACGGAG TATGACGAAA 301 GTTTTCTTTG ATCTTTAAAA TAATTTAATT TTAATATACA TTTAAAATAC TGAAGCTCCA 361 CAATTTGGTG AATTATCAAA TGTATGCTTT TTTATTTCTA GATAACTTTA TCATAGTACC 421 ATGTGACAGA TATAGTACTA GAGTGGCAGG TGTTAGAACA AGATCATGGA TGATTTTCTT 481 TTAATGGCAT AATTATTTTT TTTCAGTTAA TTACAGATGC TATCATGATG GTGAAGATAA 541 GCCAGCCAAT CATTTTATTG GGGAGGTAAT TTAAAATAAA TCTTTTTCTA AGCATTTTAG 601 AGAGTTTCTA GGAACTGTTA CTGCTCTTAG GGGGCATACT ACAACTTTTC AAAAGATTTA 661 GTTTTCTAAT GCATGTTAAC AGAGTGAAGG GGCACATGTG CTTCACATAG AAATCAGTTC 721 CACAAACATT TATGGAGTAA CTTTCTGAGT AATGTGGGGG ATCTAGAAAT AGATAAGACT 781 AGGTACTTGT CCTTAAAGAG TTTGGTCTAT CAGAAGAAGA AAGCAATGTA AGGACTGACT 841 ATATAGCAGA ATAGACAAGT GGTACTAAGA CATTAATTCC AGTGGAGGCA TAAGAGCAGA 901 ATCTTCCAAA GGCAGTTGGA GCAAGGCATT GAGGAATAGG AGGGCTTCAA TAGAGGAAGA 961 TGAAAGAGAA CAGCACATTC CAGATAGGAA CAGTATTCAA GGCCACTCAA GTTTGTAAAG 1021 TTAAGTATGT TTATGCTCCA GAAAGTGGTG CACTGCAGCT GCTTGGTTGG GGGTGGGAGG 1081 GAGTGTATCT AATGCAAAGT TGTTATGCCT AGTGGCAAAG AGCCATCTGG GATCCACTCT 1141 GATCAGCCCA CTGTTGCTCA TGCTCTTTAA TACCAAAAGA // |
Primer: pAxCALNL_F1 Sequence file: RDB07322_A3I5c.seq |
>07322_07322_A3I5_pAxCALNL_F1_G11_20_ABI24.ab1 1 ATAAGGCTAT CTTATCATGT CTGCTCGAAG CGGCCGGCCG CCCCGACTCT AGAATTATTA 61 CACGGCGATC TTGCCGCCCT TCTTGGCCTT AATGAGAATC TCGCGGATCT TGCGGGCGTC 121 CAACTTGCCG GTCAGTCCTT TAGGCACCTC GTCCACGAAC ACAACACCAC CGCGCAGCTT 181 CTTGGCGGTT GTAACCTGGC TGGCCACATA GTCCACGATC TCCTTCTCGG TCATGGTTTT 241 ACCGTGTTCC AGCACGACGA CTGCGGCGGG CAGCTCGCCG GCATCGTCGT CGGGCAGGCC 301 GGCGACCCCG GCGTCGAAGA TGTTGGGGTG TTGCAGCAGG ATGCTCTCCA GTTCGGCTGG 361 GGCTACCTGG TAGCCCTTGT ATTTGATCAG GCTCTTCAGC CGGTCCACGA TGAAGAAGTG 421 CTCGTCCTCG TCCCAGTAGG CGATGTCGCC GCTGTGCAGC CAGCCGTCCT TGTCGATGAG 481 AGCGTTTGTA GCCTCGGGGT TGTTAACGTA GCCGCTCATG ATCATGGGGC CACGGACGCA 541 CAGCTCGCCG CGCTGGTTCA CACCCAGTGT CTTACCGGTG TCCAAGTCCA CCACCTTAGC 601 CTCGAAGAAG GGCACCACCT TGCCTACTGC GCCAGGCTTG TCGTCCCCTT CGGGGGTGAT 661 CAGAATGGCG CTGGTTGTTT CTGTCAGGCC GTAGCCCTGG CGGATGCCTG GTAGGTGGAA 721 GCGTTTGGCC ACGGCCTCAC CTACCTCATT GCTGAGCGGC GCCCCGCCGC TGGCGATCTC 781 GTGCAAGTTG CTTAGGTCGT ACTTGTCGAT GAGAGTGCTC TTAGCGAAGA AGCTAAATAG 841 TGTGGGCACC AGCAGGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC 901 CTCCTCGAAG CGGTACATGA GCACGACCCG AAAGCCGCAG ATCAAGTAGC CCAGCGTGGT 961 GAACATGCCG AAAGCCGTGG TGAAATGGCA CCACGCTGAG GATAGCGGTG TCGGGGATGA 1021 TCTGGGTTGC CGAAGATGGG GTCGCGGGCA TGACTGATCG GACACAAGCG GGTGCGTTGC 1081 GGTAGGCTAC GCCCATTGGG GCCAATTCGT ACTGGCACTT ACTGTTCAAT GATCAAGGGC 1141 GAATGGTTTT TTGTGTTCCC CGGGGTC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
user_report | Suzuki, T., Arsenite-induced histone H3 modification and its effects on EGR1 and FOS expression in HeLa cells. J. Appl. Toxicol. 38 (5): 734-743 (2018) PMID 29350772. [link to RRC of NBRP] |
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