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pGL4-phHSP90AB1(HSPCB) (#RDB07321)

Promoter collection, Human HSP90AB1 promoter

Alternative name U2678(2056)-1
Clone info. PCR amplified human HSP90AB1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 44245932 to 44247147(NC_000006.12); relatively -1026 to +190, where +1 corresponds to 1 nt of NM_001271969.1.
Comment PCR cloning, forward primer: AGGCTGAAGGGGAACAACTT; reverse primer: TTCCGATACCCAGACAGTCC. Known differences (at least): Substitutions C to T at 44,246,588, T to C at 44,246,956, A to G at 44,247,121 as compared with NC_000006.12.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human heat shock protein 90kDa alpha (cytosolic), class B member 1 genomic DNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07321.pdf

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07321 pGL4-phHSP90AB1(HSPCB) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phHSP90AB1(HSPCB) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07321).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07321_A7Eop1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07321_A7Eoa.seq check
>RDB07321_07321_A7Eo_pGL4-4174F_H01_22_ABI24.ab1
    1 ATTGGGGTCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGG
   61 ATAGGCTGAA GGGGAACAAC TTGGCCAGCC GCTCTGTGGC CGGCATCGTC ACGCGGCCTC
  121 CAAGAGCTCC GGCTGCCCTG CACTGGTTCC CAGAGACTCC CTCCTTCCCA GGTCCAAATG
  181 GCTGCAGGAG CGAAGTGGGC GGAAAAAAAG CGAACCAGCT TGAGAAAGGG CTTGACGTGC
  241 CTGCGTAGGG AGGGCGCATG TCCCCGTGCT CCGTGTACGT GGCGGCCGCA GGGGCTAGAG
  301 GGGGGTCCCC CCCGCAGGTA CTCCACTCTC AGTCTGCAAA AGTGTACGCC CGCAGAGCCG
  361 CCCCAGGTGC CTGGGTGTTG TGTGATTGAC GCGGGGAAGG AGGGGTCAGC CGATCCCTCC
  421 CCAACCCTCC ATCCCATCCC TGAGGATTGG GCTGGTACCC GCGTCTCTCG GACAGGTCAG
  481 AGCGGGTCGC CGGGTGGGGT CGCTGCAAAA ACCCTGCCCC GGCCGCAGCC GGAGAGGCGG
  541 AGCCTCGCGG GGAGGGGGCG GGACCGCCGA GACAGGCCTG GAAACTGCTG GAAATGCCGC
  601 AGTGCCGCCG CCGCGCCTTC CGCCGCATGT CGGCAAAGAG TCCCCGCCAG CCCCGGCCGG
  661 CGCCCTCCCC CTACGCTGAG CTGCCCCTCA GCGCGAACCC TCCGCCCTTC CTCTACTCTT
  721 GCGAGAGTCG GGATCTGGGG CTACCCAAGG TTGGGTCCCG AATGCCAGTC CCTCTGTCGG
  781 GACGCGAGAT GTGTAGGGCA GATGCTAGGA AGAAGATTGG GTCTGGGAGC GGTGGTCCGC
  841 GTGGTTAGCT GCCTCCGCTC TTTTTCGGTG TCCCCCCCAG TCCCGCCCTT GGGTGTGGGG
  901 ACGCCTGCCC CACAAGTGTT TAGGGAGGTC AGTGGGTTCC TCGCCCGTAG AGACACCGTT
  961 TATGCCAAAT GAGCACTCCT CATCCCCGCT CTTGATGGAG TCATGTCCTA GACGTGAAAC
 1021 TATGGGGGCT GTGATCACAA GCAATGTGTG GGCGGGATCC GTTTGCTTGG GGTTCTCCCC
 1081 GCCCCCTCGT TTTTTCGGAC CATGGACGTT CAGGTTGGGC CTGGTTGGGC GGCCAGGTGG
1141 CGGGGGTTGA ACAATTTCAA TTCGT
//
Primer: pGL4-136R
Sequence file: RDB07321_A7Eob.seq check
>RDB07321_07321_A7Eo_pGL4-136R_A02_02_ABI24.ab1
    1 GCTCTTAGTT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
   61 GGCCGCCGAG GCCAGATCTT GATTTCCGAT ACCCAGACAG TCCCAACACC GCGCCGGAGT
  121 GACTCGAGAG AGCTACTGCG TGCCCCAAGT CGCCCTATAT AAGGCGAAGC ACAGTACAGC
  181 CGCCCTCCTG TAGATCCCTC CGCCTTAAAG GAGTATGATT GTCAACCCCG CACCTGCCGC
  241 CACCAGCCCA CCTTGACGTC ATGGTCCGAA AAAAGGAGGG GGCGGGGAAG AACCCAAGCA
  301 ACGGATCCGC CCACACATTT GCTTGTGATC ACAGCCCCAT AGTTTCACGT CTAGGACATG
  361 ACTCCATCAA GAGCGGGGAT GAGGAGTGCT CATTTGGCAT AAACGGTGTC TCTACGGGCG
  421 AGGAACCCAC TGACCTCCCT AAACACTTGT GGGGCAGGCG TCCCCACACC CAAGGGCGGG
  481 ACTGGGGGGG ACACCGAAAA AGAGCGGAGG CAGCTAACCA CGCGGACCAC CGCTCCCAGA
  541 CCCAATCTTC TTCCTAGCAT CTGCCCTACA CATCTCGCGT CCCGACAGAG GGACTGGCAT
  601 TCGGGACCCA ACCTTGGGTA GCCCCAGATC CCGACTCTCG CAAGAGTAGA GGAAGGGCGG
  661 AGGGTTCGCG CTGAGGGGCA GCTCAGCGTA GGGGGAGGGC GCCGGCCGGG GCTGGCGGGG
  721 ACTCTTTGCC GACATGCGGC GGAAGGCGCG GCGGCGGCAC TGCGGCATTT CCAGCAGTTT
  781 CCAGGCCTGT CTCGGCGGTC CCGCCCCCTC CCCGCGAGGC TCCGCCTCTC CGGCTGCGGC
  841 CGGGGCAGGG TTTTTGCAGC GACCCCACCC GGCGACCCGC TCTGACCTGT CCGAGAGACG
  901 CGGGTACCAG CCCAATCCTC AGGGATGGGA TGGAGTTGGG GGGGAGAGGG GATCGGCTGA
  961 ACCCCTTCCT TTCCCCGCGT CAATCACACA ACACCCAGGG CACCCTGGGG GCGGGCTCTG
 1021 CGGGCGTACA CTTTTTGCAG ACTGAGAGTG AGGTACCCTG CGGGGGGGGA CCCCCCCTCT
 1081 AGCCCCTGCG CCGCCACGTA CACCGGAGCA CGGGACATGC GCCCTTCCTT ACGCAGGCAC
1141 GGTTCAGAGG CCCTTTTCCT CTCA
//
Primer: pAxCALNL_F1
Sequence file: RDB07321_A7Eoc.seq check
>RDB07321_07321_A7Eo_pAxCALNL_F1_B02_05_ABI24.ab1
    1 CTTATGTATC TTATCATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTATTAC
   61 ACGGCGATCT TGCCGCCCTT CTTGGCCTTA ATGAGAATCT CGCGGATCTT GCGGGCGTCC
  121 AACTTGCCGG TCAGTCCTTT AGGCACCTCG TCCACGAACA CAACACCACC GCGCAGCTTC
  181 TTGGCGGTTG TAACCTGGCT GGCCACATAG TCCACGATCT CCTTCTCGGT CATGGTTTTA
  241 CCGTGTTCCA GCACGACGAC TGCGGCGGGC AGCTCGCCGG CATCGTCGTC GGGCAGGCCG
  301 GCGACCCCGG CGTCGAAGAT GTTGGGGTGT TGCAGCAGGA TGCTCTCCAG TTCGGCTGGG
  361 GCTACCTGGT AGCCCTTGTA TTTGATCAGG CTCTTCAGCC GGTCCACGAT GAAGAAGTGC
  421 TCGTCCTCGT CCCAGTAGGC GATGTCGCCG CTGTGCAGCC AGCCGTCCTT GTCGATGAGA
  481 GCGTTTGTAG CCTCGGGGTT GTTAACGTAG CCGCTCATGA TCATGGGGCC ACGGACGCAC
  541 AGCTCGCCGC GCTGGTTCAC ACCCAGTGTC TTACCGGTGT CCAAGTCCAC CACCTTAGCC
  601 TCGAAGAAGG GCACCACCTT GCCTACTGCG CCAGGCTTGT CGTCCCCTTC GGGGGTGATC
  661 AGAATGGCGC TGGTTGTTTC TGTCAGGCCG TAGCCCTGGC GGATGCCTGG TAGGTGGAAG
  721 CGTTTGGCCA CGGCCTCACC TACCTCCTTG CTGAGCGGCG CCCCGCCGCT GGCGATCTCG
  781 TGCAAGTTGC TTAGGTCGTA CTTGTCGATG AGAGTGCTCT TAGCGAAGAA GCTAAATAGT
  841 GTGGGCACCA GCAGGGCAGA TTGAATCTTA TAGTCTTGCA AGCTGCGCAA GAATAGCTCC
  901 TCCTCGAAGC GGTACATGAG CACGACCCGA AAGCCGCAGA TCAAGTAGCC CAGCGTGGTG
  961 AACATGCCGA AGCCGTGGTG AAATGGCACC ACGCTGAGGA TAGCGGGTGT CGGGGGATGA
 1021 TCTGGTTTGC CGAAGATGGG GTCGCGGGCA TGACTGAATC GGGACACAGG CGGTGCGTGC
1081 GTAGGCTACG CCTGGCAATT CAGTTACTGC CACTACTGAT CATGATCCAA GGGCCGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles