Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL4-phAPCS (#RDB07317)

Promoter collection, Human APCS promoter

Alternative name	U2671(2049)-7
Clone info.	PCR amplified human APCS promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 159586692 to 159587854(NC_000001.11); relatively -1134 to +29, where +1 corresponds to 1 nt of NM_001639.4.
Comment	PCR cloning, forward primer: CTTGGTGGCAGTGATTGTGT; reverse primer: TGTCCCCCTAGCGTCTGATA. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human amyloid P component, serum genomic DNA
Depositor|Developer	DNA Bank, |

 

Remarks, protocol and/or map (pdf)	RDB07317.pdf

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In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07317	pGL4-phAPCS	DNA solution

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pGL4-phAPCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07317).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB07317_A4C4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB07317_A4C4a.seq
>07317_07317_A4C4_3_pGL4-4174F_C07_07.ab1     1 GACGGGGGTC AGGACATTTC TCGTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG    61 AGGATCTTGG TGGCAGTGAT TGTGTCATTG AAAAGGCTGA TTAGGTCTGT GGTGAGCTAA   121 CAGAAGAGAG AGTGAACAAG AGGCTAAACA GTAGGATGAG AAACAAGACA CCATATAATT   181 CAGGGATGAG AGAGAAAGAA ATAATGTGGC TAGAAAGAAG AACTTCAGTT TGATATCTCA   241 GAGGCAGAGA AGGTTTGAGT GGTGGTTAAT AAACTGTACA GAGAGAGAAT GAAGGAGACA   301 TAGAAAGAAT GAAGGTGGAG ATCTCTGGGG CTGTGAGAGA GCCAGTGACC ACTGAATCCC   361 CAGCACCTAG TGTAGTAACT GAGAGCTAGT GAATAAATGT ATTAGTGGGG TCATCACCAA   421 AGATGGATTA GATATACACT CAATGACCAA GGACTCAAAG AAGAGGAGAG AAAAACTTGG   481 GGAGATGCTG ACATCTCTGC AGGTAGCATG AAGTAACCAG AAAACAAGTC GTAATGAGAA   541 ATGGAAAGAT CTAGGATAAG GAAGCACCAT GATATGCAAA CGAAAAAAAA GCACTGAGTA   601 GAAGTAGCAG AAAAATACTA AGCTTCATGG GGACAGAAAG TTTATTCTCC AAGTCCCTCT   661 TTCCCCTTAG CTTTGCCTGG ATGTATTACT TGATGTTGAA TTAGTCAAAG TTGAAGGCAC   721 TTTTTAGGGT CTTCAGCAGA AAGCAGAATC CTAGAATATC CTACAAATAC AAGACAACTT   781 GAGAACTATA AAAGAAAGAA AAGGCCTTGT TCCCATAGCC CACAAGAGGG CTGAGCTCAG   841 CGATACACTC TGTGTGGCTC AGGAGCTTCT GGCAGAGCAC AGAGCCATCA GAGAGGGGGG   901 CCCAGAGAGC AGAGAGAGGC TCTGCTGACA TGGCCAGCCC AGCGCCTCAT CCCATCCATA   961 ACCCTGCTGC CCTGCAATGA CTCTCTTTCC CAGCAATAAA ATCTGGGTCA CAGGAGTTGG  1021 GAGCTCTAGC CATCACTTGT CTCTAATAAA TAACTCCCAT TGATTTTCCA GCTCATGCTC  1081 ACACTCCTTT CACGGTAGCG TAAGCAGCAG ACCTGGCAAA AATCACCTCC ACCAATAATT 1141 AATTTGGGGG TGGC //
Primer: Sequence file: RDB07317_A4C4b.seq
>07317_07317_A4C4_3_pGL4-136R_F07_16.ab1     1 GCTCTTTAGT TTTTGGCATC TTCCATGGTG GCTTTACCAA CAGTACCGGA TTGCCAAGCT    61 TGGCCGCCGA GGCCAGATCT TGATTGTCCC CCTAGCGTCT GATATTCATG CCCTTAGACT   121 TCAGGTTATT CAGGGTTTAT ATGCACCTTG GGTGAGGATG GGGGAGGAAG AGCACCAATA   181 ATATGTGAGT GATTTTCCAG GTCTGCCTCC TGGCGCTTAC GGTGAAGGAG TGGTGAGCCC   241 TGAGCTGGAA AATCAATGGG AGTTATTTAT TAGAGACAAG TGATGGCTAG AGCTCCAACT   301 CCTGTGACCC AGATTTTATT GCTGGGAAGA GAGTCATTGC AGGGCAGCAG GGTTATGGAT   361 GGGATGAGGC GCTGGGCTGG CCATGTCAGC AGAGCCTCTC TCTGCTCTCT GGGCCCCCTC   421 TCTGATGGCT CTGTGCTCTG CCAGAAGCTC CTGAGCCACA CAGAGTGTAT CGCTGAGCTC   481 AGCCCTCTTG TGGGCTATGG GAACAAGGCC TTTTCTTTCT TTTATAGTTC TCAAGTTGTC   541 TTGTATTTGT AGGATATTCT AGGATTCTGC TTTCTGCTGA AGACCCTAAA AAGTGCCTTC   601 AACTTTGACT AATTCAACAT CAAGTAATAC ATCCAGGCAA AGCTAAGGGG AAAGAGGGAC   661 TTGGAGAATA AACTTTCTGT CCCCATGAAG CTTAGTATTT TTCTGCTACT TCTACTCAGT   721 GCTTTTTTTT CGTTTGCATA TCATGGTGCT TCCTTATCCT AGATCTTTCC ATTTCTCATT   781 ACGACTTGTT TTCTGGTTAC TTCATGCTAC CTGCAGAGAT GTCAGCATCT CCCCAAGTTT   841 TTCTCTCCTC TTCTTTGAGT CCTTGGTCAT TGAGTGTATA TCTAATCCAT CTTTGGTGAT   901 GACCCCACTA ATACATTTAT TCACTAGCTC TCAGTTACTA CACTAGGTGC TGGGGGATTC   961 AGTGGTCACT GGCTCTCTCA CAGCCCCAGA GATCTCCACC TTCATTCTTT CTATGTCTCC  1021 CTTCATTCTC TCTCTGTACA GTTATTTAAA CCACCACTCA AAACCCTTCT CTGCCTCTGA  1081 GAATATCAAA CTGGCAGGTC GTCGTTTCTA GGCCACATAT TTCGTTTCCT CTCTCAATTC 1141 CCCTGTGAT //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Link

Other resources in our bank

• human amyloid P component, serum

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External Database
Human amyloid P component, serum

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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Featured content

• Promoter Collection (Firefly Luciferase) (English text)

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