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pGL4-phMMP2 (#RDB07314)

Promoter collection, Human MMP2 promoter

Alternative name U2667 (2045)-1
Clone info. PCR amplified human MMP2 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 55470229 to 55479221(NC_000016.10); relatively -8969 to +24, where +1 corresponds to 1 nt of NM_004530.6.
Comment PCR cloning, forward primer: ctgcctcagcctcctgagta; reverse primer: cgcctgaggaagtctggat. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human matrix metallopeptidase 2 genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
 
Remarks, protocol and/or map (pdf) RDB07314.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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RDB07314 pGL4-phMMP2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phMMP2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07314).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12746_A4D2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12746_A4D2a.seq check
>07314_12746_A4D2_pGL4-4174F_A11_02.ab1
    1 AGGTGGGTTC CCGGAAAATT TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC
   61 GAGGATTTCC TCAGAGCCGG TTGTCACAGG GAGAACTTCA AAATTGGGAT TCGACCTGAG
  121 AGGCCACATG GATTCTTGGC TTGGCGCAGG AAAGGATTCA AGAGTGAGTG GGGAATTCGT
  181 GGAACTGAGG GCTCCTCCCC TTTTTAGACC ATATAGGGTA AACCTCCCCA CATTGCCATG
  241 GCATTTATAC ACTGCCATGG CACTGGTGGG TGCTTCCTTT AACATGCTAA TGCATTATAA
  301 TTAGCGTAAA ATGAGCAGTG AGGATGACCA GAGGTCGCTT TCTTTGCCAT CTTGGTTTTG
  361 GCTGGCTTCT TCACTGCATA CTGTTTTATC AGTGGGGTCT TTGTGACCTC TATCTTATTA
  421 AACCAGTCTT GCCCAATTTC TATCTCATCC TGTGACCGAG AATGCGGACC CTCCTGGGAG
  481 TGCAGCCCAG CAGGTCTCAG CCTCATTTTA CCCAGCCCCC TGTTCAAGAT GGAGTCGCTC
  541 TGGTTCCAAC GTCTCTAACG CGGGGCCCCT GACTGCTCTA TTTCCCAAGG TGTATCTAGC
  601 ATCTCGCACT ATACGAGGCC AAGTTAAGGC TTACACATTT GCAGAAGGAA AGAGGTAAGG
  661 AAGCAACCTG GGACCTTCCA CTGTCTCTGT TTCCATCTCT CTCTTTCCAT CTCTGTTCAT
  721 CCCAGAATCT CTCTGTCCCT ATCCCTAAAT ATCGAAAATT TCTGTCTCTG ACCATCTATC
  781 ATTGTGGCTG ATCATCTGTT TCTGACCATT CCTTCCCGTT CCTGACCCCA GGGAGTGCAG
  841 GGTGTCCTAG CCAAGCCGGC GTCCCTCCTA GTAGTACCGC TGCTCTCTAA CCTCAGGACG
  901 TCAAAGGGCC TAGAGCGACA GATGTTTCCC AGCAGGGGGT TCTGAGGCTG TGCGCCCAGA
  961 TCGCGAGAGA GGGCAAGTGG GGGTGACGAG GTCGTGCACT GAGGGTGGAC GTAGAGGCCC
 1021 AGGAGTAGCA GGCGGGCCGG GGGAAAAGAG TGGAGAAAGG AAAAAAGGAG GAGAAAGTGG
1081 AGGGAGGGCG AGTAAGGGGG GGGGTGGGGG GGCCAGAAAG GAGAGGGCCC
//
Primer:
Sequence file: RDB12746_A4D2b.seq check
>07314_12746_A4D2_pGL4-136R_G11_20.ab1
    1 CTTTAGTTGT TTTTTGGGCA TCTTCCATGG TGGCTTTACC AACAGTACCG GATTGCCAAG
   61 CTTGGCCGCC GAGGCCAGAT CTTGATCGCC TGAGGAAGTC TGGATGCAGC GGAAACAAGG
  121 GAGGGCAGCC GCCAGATGTA GCCGGCTGGG CTGGGAGGGA GCTGGCAGAG CGGGGCTGGG
  181 GGCGGGGGGC GCACTCGGGC CCGCCCCTCT CTGCCCCACC CCCCTACTCG CCCTCCTCCA
  241 CTTTTCTCCT CTTTTTTCCT TTCTCCACCT CTTTTCCCCG GCCGCCTGCT ACTCCTGGCC
  301 TCTACGTCCA CCCTCAGTGC ACGACCTCGT CACCCCACTT GCCTCTCTCG CGATCTGGGC
  361 GCACAGCCTC AGAACCCCCT GCTGGGAAAC ATCTGTCGCT CTAGGCCCTT GACGTCCTGA
  421 GGTTAGAGAG CAGCGGTACT ACTAGGAGGG ACGCCGGCTT GGCTAGGACA CCCTGCACTC
  481 CCTGGGGTCA GGAACGGGAA GGAATGGTCA GAAACAGATG ATCAGCCACA ATGATAGATG
  541 GTCAGAGACA GAAATTTTCG ATATTTAGGG ATAGGGACAG AGAGATTCTG GGATGAACAG
  601 AGATGGAAAG AGAGAGATGG AAACAGAGAC AGTGGAAGGT CCCAGGTTGC TTCCTTACCT
  661 CTTTCCTTCT GCAAATGTGT AAGCCTTAAC TTGGCCTCGT ATAGTGCGAG ATGCTAGATA
  721 CACCTTGGGA AATAGAGCAG TCAGGGGCCC CGCGTTAGAG ACGTTGGAAC CAGAGCGACT
  781 CCATCTTGAA CAGGGGGCTG GGTAAAATGA GGCTGAGACC TGCTGGGCTG CACTCCCAGG
  841 AGGGTCCGCA TTCTCGGTCA CAGGATGAGA TAGAAATTGG GCAAGACTGG TTTAATAAGA
  901 TAGAGGTCAC AAAGACCCCC ACTGATAAAA CAGTATGCAG TGAAGAAGCC AGCCAAAACC
  961 AAGATGGCAA AGAAAGCGAC CTCTGGTCAT CCTCACTGCT CATTTTACGC TAATTATAAT
 1021 GCATTAGCAT GTTTAAAGGG AAGCACCCAC CAGTGCCCAT GGCCAGTGTA TAAATGGCCC
 1081 ATTGCCAATG TGGGGGAGTT TTACCCTATA TGTCTAAAAA GGGTAGAGCC CCCTCCAGTT
1141 CCCCACGGAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles