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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phCDKN1A

Promoter collection, Human CDKN1A promoter

Catalog number RDB07302
Resource name pGL4-phCDKN1A
Alternative name U2651 (2029)-4
Clone info. PCR amplified human CDKN1A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 36677551 to 36678753(NC_000006.12); relatively -1128 to +75, where +1 corresponds to 1 nt of NM_000389.4.
Comment PCR cloning, forward primer: atgggagcggatagacacat; reverse primer: ggctccacaaggaactgact. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
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External Database
Human cyclin-dependent kinase inhibitor 1A (p21, Cip1)

          Reference sequence
            

          Distribution information

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          RDB07302 pGL4-phCDKN1A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07302.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07302_A3Go.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB07302_A3Goa.seq check
          >07302_07302_A3Go_pGL4-4174F_E10_13.ab1
              1 ATTGGGGGCC AGACATTTCT CTGGCCTAAC TGGCCGGTAC CTGAGCTCGC TAGCCTCGAG
             61 GATATGGGAG CGGATAGACA CATCACTCAT TTCTGTGTCT GTCAGAAGAA CCAGTAGACA
            121 CTTCCAGAAT TGTCCTTTAT TTATGTCATC TCCATAAACC ATCTGCAAAT GAGGGTTATT
            181 TGGCATTTTT GTCATTTTGG AGCCACAGAA ATAAAGGATG ACAAGCAGAG AGCCCCGGGC
            241 AGGAGGCAAA AGTCCTGTGT TCCAACTATA GTCATTTCTT TGCTGCATGA TCTGAGTTAG
            301 GTCACCAGAC TTCTCTGAGC CCCAGTTTCC CCAGCAGTGT ATACGGGCTA TGTGGGGAGT
            361 ATTCAGGAGA CAGACAACTC ACTCGTCAAA TCCTCCCCTT CCTGGCCAAC AAAGCTGCTG
            421 CAACCACAGG GATTTCTTCT GTTCAGGTGA GTGTAGGGTG TAGGGAGATT GGTTCAATGT
            481 CCAATTCTTC TGTTTCCCTG GAGATCAGGT TGCCCTTTTT TGGTAGTCTC TCCAATTCCC
            541 TCCTTCCCGG AAGCATGTGA CAATCAACAA CTTTGTATAC TTAAGTTCAG TGGACCTCAA
            601 TTTCCTCATC TGTGAAATAA ACGGGACTGA AAAATCATTC TGGCCTCAAG ATGCTTTGTT
          661 GGGGTGTCTA GGTGC
          //
          Primer:
          Sequence file: RDB07302_A3Gob.seq check
          >07302_07302_A3Go_pGL4-136R_F10_16.ab1
              1 GGTCCTTAAG CTTTGGCATC TTCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
             61 GGCCGCCGAG GCCAGATCTT GATGGCTCCA CAAGGAACTG ACTTCGGCAG CTGCTCACAC
            121 CTCAGCTGGC GCAGCTCAGC GCGGCCCTGA TATACAACCG CCCCGCCCGG GCCCGCCTCA
            181 AGGAGGCGGG ACCCGCGCTC GGCCCACCGC GCCGCCCGGG ACCGCCCTCC CCGGGGCCCA
            241 GGCTCCTGGC TGCCCAGCGC CGAGCCAGCT GAGCCTGGCC GAGTTCCAGC AGGCCAGCCG
            301 GTCCCGGAAC CTCGCGTGCT GCAGGAGGGC ACTTCCCTCC TCCCCCAGTC CCTCGCCTGC
            361 GTTGGTGCGC TGGACACATT TCCCCACGAA GTGAGCCACA AATCTGGCTT TTTTTACTTG
            421 GAGAATGAGT TGGCACTCTC CAGGAGGACA CAGCACTGTT AGAATGAGCC CCCTTTCTGG
            481 CTCACCGCTG ACCCACTCTG GCAGGCAAGG ATTTACCCAA TGCAGCTGAA AAGATCAGGA
            541 GGATGACATT AATACATAAA AATTCATAAA TTATAAAAAC GATGCACCTC TCTGCAATTT
            601 CCAGAAAAGC CCCACAATAT CACCTCTATT CCCACTGATC CCTCACTAGG TCACCTCTCC
          661 CAGAAGCACC TGGAGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl