Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phCCND1 (#RDB07299)

Promoter collection, Human CCND1 promoter

Alternative name U2647 (2025)-1
Clone info. PCR amplified human CCND1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 69639958 to 69641148(NC_000011.10); relatively -1147 to +44, where +1 corresponds to 1 nt of NM_053056.2.
Comment PCR cloning, forward primer: acgaacacctatcgattttgct; reverse primer: ccaggactttgcaacttcaac. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human cyclin D1 genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
 
Remarks, protocol and/or map (pdf) RDB07299.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 謝辞の表明を必要とする。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07299 pGL4-phCCND1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phCCND1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07299).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19760_B2Jlp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4ins_F (Pr0665)
Region: pause site,insert 5'
Sequence file: RDB19760_B2Jla.seq check
>07299_19760_B2Jl_2_pGL4ins_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGCAAAN TAGGCTGTCC CCAGTGCAAN GTGCAGGTGC
   61 CAGAACATTT CTCTGGCCTA ACTGGCCGGT ACCTGAGCTC GCTAGCCTCG AGGATACGAA
  121 CACCTATCGA TTTTGCTAAA AATCAGATCA GTACACTCGT TTGTTTAATT GATAATTGTT
  181 CTGAATTATG CCGGCTCCTG CCAGCCCCCT CACGCTCACG AATTCAGTCC CAGGGCAAAT
  241 TCTAAAGGTG AAGGGACGTC TACACCCCCA ACAAAACCAA TTAGGAACCT TCGGTGGTCT
  301 TGTCCCAGGC AGAGGGGACT AATATTTCCA GCAATTTAAT TTCTTTTTTA ATTAAAAAAA
  361 ATGAGTCAGA ATGGAGATCA CTGTTTCTCA GCTTTCCATT CAGAGGTGTG TTTCTCCCGG
  421 TTAAATTGCC GGCACGGGAA GGGAGGGGGT GCAGTTGGGG ACCCCCGCAA GGACCGACTG
  481 GTCAAGGTAG GAAGGCAGCC CGAAGAGTCT CCAGGCTAGA AGGACAAGAT GAAGGAAATG
  541 CTGGCCACCA TCTTGGGCTG CTGCTGGAAT TTTCGGGCAT TTATTTTATT TTATTTTTTG
  601 AGCGAGCGCA TGCTAAGCTG AAATCCCTTT AACTTTTAGG GTTACCCCCT TGGGCATTTG
  661 CAACGACGCC CCTGTGCGCC GGAATGAAAC TTGCACAGGG GTTGTGTGCC CGGTCCTCCC
  721 CGTCCTTGCA TGCTAAATTA GTTCTTGCAA TTTACACGTG TTAATGAAAA ATGAAAGANN
  781 ATGCAGTCGC TGANATTCTT TTGNCCGTCT GTCCNCCCGT GGGTGCCCTC GTGGCGTTCT
  841 TGGAAANTGC GCCCATTCTG CCNGCTTGGA TATGGGGNGT CGCCNNCNNC CCANTCACCC
  901 CNTNNNCGNG GNNNTNCCNN NNNCNGCGTG NGGNCNTGNC NNGNNNNNCN TANTTGNCCC
961 CNNNNNNNNN N
//
Primer: pGL4ins_R (Pr0666)
Region: luc,insert 3'
Sequence file: RDB19760_B2Jlb.seq check
>07299_19760_B2Jl_2_pGL4ins_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGGN NNNNNGGCGC TGGGCCCTTC TTANNTGTTT
   61 TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG GCCGCCGAGG
  121 CCAGATCTTG ATCCAGGACT TTGCAACTTC AACAAAACTC CCCTGTAGTC CGTGTGACGT
  181 TACTGTTGTT AAGCAAAGAT CAAAGCCCGG CAGAGAATGG GAGCGGGAGG GGGGCGGGGG
  241 CGGGCGCAGG GGGAGGGGGC GCGGGCGCCA AACGCCGGGA GCAGCGAGGG GCAGAGCCCA
  301 AAAGCCATCC CTGAGGCGCC GCGCCTGCCC TGCGGCGGAG TTGCCCCTGT AGTCCGGTTT
  361 TCATAGAAAT GCAAATCGCC CCGCTGCAGC CTTTCTCCCC GCCAGGGAAG AGGGGTGCAG
  421 GGGGCCCCGT CCGCCTCGAG TGGGTCCCCC GGGATTTAGG GGGTGAGGTG GAGGTGGCTC
  481 TGCAGTAGGG GACAACTAGG AAGGCCGGCA GGCCACACGC AGCCTGGGGA GACCACGAGA
  541 AGGGGTGACT GGGGCGCGGC GACACCCCAT ATCCAAGCCG GCAGAATGGG CGCATTTCCA
  601 AGAACGCCAC GAGGGCACCC ACGGGCGGAC AGACGGCCAA AGAATCTCAG CGACTGCATC
  661 TTCTTTCATT TTCATTAACA CGTGTAAATT GCAAGAACTA ATTTAGCATG CAAGGACGGG
  721 GAGGACCGGG CACACAACCC CTGTGCAAGT TTCATTCCGG CGCACAGGGG CGTCGTTGCN
  781 AAATGCCCAN NGNNGGTAAC CCTAAAAGTT AAAGGGGNNT TCAGCTTANC ATGCGCTCGC
  841 TCAAAAAAAN NAAAANNAAA ATNAATGNCC CNAAAATTNC NNNNNANCAG CCNNANNAAN
  901 NGGNGGGNCN AGCNNTTTTN CCNTNCATCN TTGTCCNNNC TANNCNNGGN ANANTCNTNC
  961 NGGGNTGNCN NTNNCNTNNC NNTTNNANNN NNNNNGNNCN NTNGNNNGGG GGNNNCCNNA
1021 NNNNNNNNNN
//
Primer: pGL4pA_R (Pr0664)
Region: SV40 pA,luc
Sequence file: RDB19760_B2Jlc.seq check
>07299_19760_B2Jl_2_pGL4pA_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTAT CGNNTTTACN NCATTTGTAG AGGTTTTACT
   61 TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
  121 TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
  181 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA
  241 TGTATCTTAT CATGTCTGCT CGAAGCGGCC GGCCGCCCCG ACTCTAGAAT TATTACACGG
  301 CGATCTTGCC GCCCTTCTTG GCCTTAATGA GAATCTCGCG GATCTTGCGG GCGTCCAACT
  361 TGCCGGTCAG TCCTTTAGGC ACCTCGTCCA CGAACACAAC ACCACCGCGC AGCTTCTTGG
  421 CGGTTGTAAC CTGGCTGGCC ACATAGTCCA CGATCTCCTT CTCGGTCATG GTTTTACCGT
  481 GTTCCAGCAC GACGACTGCG GCGGGCAGCT CGCCGGCATC GTCGTCGGGC AGGCCGGCGA
  541 CCCCGGCGTC GAAGATGTTG GGGTGTTGCA GCAGGATGCT CTCCAGTTCG GCTGGGGCTA
  601 CCTGGTAGCC CTTGTATTTG ATCAGGCTCT TCAGCCGGTC CACGATGAAG AAGTGCTCGT
  661 CCTCGTCCCA GTAGGCGATG TCGCCGCTGT GCANNCAGCC GTCCTTGTCG ATGANAGCGT
  721 TTGTAGCCTC GGGGTTGTTN AACGTANCCG CTCATGATCA TGGGGCCACG GACGCACAGC
  781 TCGCCNCGCN NGTTCACACC CAGTGTCTTA CCGGTGTCNN AAGNNCNNCA CCTTAGCCTC
  841 GAANANNGNN ACCNCCCTNN NNNNACTGCG CCAGGCTTGT CGNNCCCNTN NNNGGNTGNN
  901 NAGAATGGCG CTNGGTNGTT TCNNNNNNGN CCGTNNNCCN NNGCNGNNNG CNNNGGTNNN
  961 NGAANCGTTT TNGNCNNNNN CNTCNCNTAN CNNNCCTTGC TGANCGGNNN NNCNNNNNCN
1021 NNNNCNNNTN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles