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pBRPyCAG-Klf4-IP

Expression clone of mouse Klf4 cDNA

Catalog number RDB07102
Resource name pBRPyCAG-Klf4-IP
Alternative name pCAG-Klf4-IP, pBRPyCAG-Klf4-IP, stock no 90173
Clone info. Expression clone of mouse Klf4 cDNA. cDNA fragment 632 to 2056nt of NM_010637.3 is cloned. Containing SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Known difference: substitutions T to C at 980nt (Ser to Pro), G to A at 1003nt (synonym), G to A at 1449nt (Cys to Tyr), and T to C at 1999nt (synoym).
Vector backbone pBRPyCAG-cHA-IP (Plasmid)
Gene/insert name Mouse Klf4 cDNA
Depositor Niwa, Hitoshi |
 
Other clones in our bank mouse Klf4 (NCBI Gene 16600) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Mol. Cell. Biol., 26, 7772-7782 (2006))
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RDB07102 pBRPyCAG-Klf4-IP DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB07102z.seq from Depositor

Featured content

References

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB07102_A8Etp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 RDB07102_A8Eta.seq
>07102_07102_A8Et_3_pAxCA_F1_A01_01_ABI08.ab1
    1 CCATTCCGTT CTTACAATGT TTCATGCCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG
   61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CCTCGAGTCT GACATGGCTG
  121 TCAGCGACGC TCTGCTCCCG TCCTTCTCCA CGTTCGCGTC CGGCCCGGCG GGAAGGGAGA
  181 AGACACTGCG TCCAGCAGGT GCCCCGACTA ACCGTTGGCG TGAGGAACTC TCTCACATGA
  241 AGCGACTTCC CCCACTTCCC GGCCGCCCCT ACGACCTGGC GGCGACGGTG GCCACAGACC
  301 TGGAGAGTGG CGGAGCTGGT GCAGCTTGCA GCAGTAACAA CCCGGCCCTC CTAGCCCGGA
  361 GGGAGACCGA GGAGTTCAAC GACCTCCTGG ACCTAGACTT TATCCTTTCC AACTCGCTAA
  421 CCCACCAGGA ATCGGTGGCC GCCACCGTGA CCACCTCGGC GCCAGCTTCA TCCTCGTCTT
  481 CCCCAGCGAG CAGCGGCCCT GCCAGCGCGC CCTCCACCTG CAGCTTCAGC TATCCGATCC
  541 GGGCCGGGGG TGACCCGGGC GTGGCTGCCA GCAACACAGG TGGAGGGCTC CTCTACAGCC
  601 GAGAATCTGC GCCACCTCCC ACGGCCCCCT TCAACCTGGC GGACATCAAT GACGTGAGCC
  661 CCTCGGGCGG CTTCGTGGCT GAGCTCCTGC GGCCGGAGTT GGACCCAGTA TACATTCCGC
  721 CACAGCAGCC TCAGCCGCCA GGTGGCGGGC TGATGGGCAA GTTTGTGCTG AAGGCGTCTC
  781 TGACCACCCC TGGCAGCGAG TACAGCAGCC CTTCGGTCAT CAGTGTTAGC AAAGGAAGCC
  841 CAGACGGCAG CCACCCCGTG GTAGTGGCGC CCTACAGCGG TGGCCCGCCG CGCATGTGCC
  901 CCAAGATTAA GCAAGAGGCG GTCCCGTCCT ACACGGTCAG CCGGTCCCTA GAGGCCCATT
  961 TGGAGCGCTG GACCCCAGCT CAGCAACGGG CCACCGGCCA ACACACACGA CTTTCCCCCT
 1021 GGGGCGGCAG CTCCCCACCA GGGACTACCC CTAACACTGA GTCCGGAGGA ACTGCTGGAC
 1081 AGCAGGACTG TCACCCTGCT GCTCTTCCCC CAGGATCATC CCATCCGGCA ACTACCTTCC
1141 TTCTGTCCAG AACTCAGAAA ATGCC
//
Primer: CSII-CMV3' RDB07102_A8Etb.seq
>07102_07102_A8Et_3_CSII-CMV3'_A02_01_ABI08.ab1
    1 GGGAAAATTC GGGAATTATC AAGCTTATCG AGCGGCCGCT TAAAAGTGCC TCTTCATGTG
   61 TAAGGCAAGG TGGTCCGACC TGGAAAAGGC CCTGTCGCAC TTCTGGCACT GAAAGGGCCG
  121 GTGCCCTGTG TGTTTGCGGT AGTGCCTGGT CAGTTCATCG GAGCGGGCGA ATTTCCACCC
  181 ACAGCCGTCC CAGTCACAGT GGTAAGGTTT CTCGCCTGTG TGAGTTCGCA GGTGTGCCTT
  241 GAGATGAGAA CTCTTGGTAT AGGTTTTGCC ACAGCCTGCA TAGTCACAAG TGTGGGTGGC
  301 TGTTCTTTTC CGGGGCCACG ACCTTCTTCC CCTCTTTGGC TTGGGCTCCT CTGGCAGGCA
  361 GGAACCCGGT GGCATGAGCT CTTGATAATG GAGAGAGGGG ACTTGTGACT GCATCTGGTC
  421 TGGCAGGAAA GGAGGGTAGT TGGGCCCCGG ATGGGGATGG AATCCTGGGG GAAGAGGCAG
  481 GCCAGGGTGA CAGTCCCTGC TGTTCAGCAG TTCCTCGGGA CTCAGTGTAG GGGTAGTCCT
  541 GGTGGGGAGC TGCCGCCCCA GGGGGAAGTC GTGTGTGTTG GGCCGGTGGC CGTTGCTGAG
  601 CTGGGGTCCA GCGCTCAAAT GGGCCTCTAG GGACCGGCTG ACCGTGTAGG ACGGGACCGC
  661 CTCTTGCTTA ATCTTGGGGC ACATGCGCGG CGGGCCACCG CTGTAGGGCG CCACTACCAC
  721 GGGGTGGCTG CCGTCTGGGC TTCCTTTGCT AACACTGATG ACCGAAAGGG CTGCTGTACT
  781 CGCTGCCAGG GGTGGTCAGA GACGCCTTCA GCACAAACTT GCCCATCAGC CCGCCACCTG
  841 GCGGCTGAGG CTGCTGTGGC GGAATGTATA CTGGGTCCAA CTCCGGCCGC AGGAGCTCAG
  901 CCACGAAGCC GCCCGAGGGG CTCACGTCAT TGATGTCCGC CAGGTTGAAG GGGGCCCGTG
  961 GGAGGTGGCG CAGATTCTCG GCTGTAGAGG AGCCTCCACC TGTGTTGCTG GCAGCCACGC
 1021 CCGGGTCACC CCCCGGCCCG GAATCGGATA GCTGAGGCTG CAGTGGAGGG CGCGCTGGCC
 1081 AGGTCGCTGC TCGCTGGGGA GGACGAAGGA ATGAAGCTTG GCGCCGAAGG GGTGGGTTCA
1141 CGGGGTTGGC GGGGC
//
Primer: CMV_en_R RDB07102_A8Etc.seq
>07102_07102_A8Et_3_CMV_en_R_A01_01_ABI08.ab1
    1 TGGGTTTAAT GATTACTATT AATAACTAGC TAGTCAATAA TCAATGTCGA CGATGATATC
   61 AGATCCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG
  121 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA CCATTATAAG
  181 CTGCAATAAA CAAGTTAATT CGATTCAGCT TCAGAAGATG GCGGAGGGCC TCCAACACAG
  241 TAATTTTCCT CCCGACTCTT AAAATAGAAA ATGTCAAGTC AGTTAAGCAG GAAGTGACTA
  301 ACTGACCGCA GCTGGCCGTG CGACATCCTC TTTTAATTAG TTGCTAGGCA ACTGCCCTCC
  361 AGAGGGCAGT GTGGTTTTGC AAGAGGAAGC AAAAAGCCTC TCCACCCAGG CCTGGAATGT
  421 CAGTGTGGTT TTGCAAGAGG AAGCAAAAAG CCTCTCCACC CAGGCCTGGA ATGTTTCCAC
  481 CCAATCATTA CTATGACAAC AGCTGTTTTT TTTAGTATTA AGCAGAGGCC GGGGGCCCCT
  541 GGCCTCCGCT TACTCTGGAG AAAAAGAAGA GAGGCTTCCA GAGGCAACTT GTCAAAACAG
  601 GACTGGCGCC TTGGAGGCGC TGTGGGGCCA CCCAAATTGA TATAATTAAG CCCCAACCGC
  661 CTCTTCCCGC CTCATTTCAG CCTCACCACT ATGTGAATAT AGCTGAATAC ACAGTTTATT
  721 GAATAAACAT TAATTTCCAG GAAATACAGT CTTTGTTTTT CCAAAGCGGT CATTGAAAAA
  781 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
  841 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
  901 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
  961 TTCGCCCCGA AGAACGTTTT CCCAATGATG AGCACTTTTT AAGTTCTGCT ATGTGGCGCG
 1021 GGTATTATCC CGTATTTGAC GCCCGGGCAA GAGCACTCGG GTCGCGGCAT AACACTAATT
 1081 CTCAGATGAC TTTGGTTGAT TACTCACAGT TCACAGAAAA GCCATCCTTA ACGGATTGGG
1141 CAATGTGAAC GAGCTAA
//
Primer: BGH_rev2 RDB07102_A8Etd.seq
>07102_07102_A8Et_3_BGH_rev2_C02_01_ABI08.ab1
    1 CATCGGGGTT AAGGAAAGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCAGC GAGCTCTAGA TCATCGATGC ATGGGGTCGT GCGCTCCTTT
  121 CGGTCGGGCG CTGCGGGTCG TGGGGCGGGC GTCAGGCACC GGGCTTGCGG GTCATGCACC
  181 AGGTGCGCGG TCCTTCGGGC ACCTCGACGT CGGCGGTGAC GGTGAAGCCG AGCCGCTCGT
  241 AGAAGGGGAG GTTGCGGGGC GCGGAGGTCT CCAGGAAGGC GGGCACCCCG GCGCGCTCGG
  301 CCGCCTCCAC TCCGGGGAGC ACGACGGCGC TGCCCAGACC CTTGCCCTGG TGGTCGGGCG
  361 AGACGCCGAC GGTGGCCAGG AACCACGCGG GCTCCTTGGG CCGGTGCGGC GCCAGGAGGC
  421 CTTCCATCTG TTGCTGCGCG GCCAGCCGGG AACCGCTCAA CTCGGCCATG CGCGGGCCGA
  481 TCTCGGCGAA CACCGCCCCC GCTTCGACGC TCTCCGGCGT GGTCCAGACC GCCACCGCGG
  541 CGCCGTCGTC CGCGACCCAC ACCTTGCCGA TGTCGAGCCC GACGCGCGTG AGGAAGAGTT
  601 CTTGCAGCTC GGTGACCCGC TCGATGTGGC GGTCCGGATC GACGGTGTGG CGCGTGGCGG
  661 GGTAGTCGGC GAACGCGGCG GCGAGGGTGC GTACGGCCCT GGGGACGTCG TCGCGGGTGG
  721 CGAGGCGCAC CGTGGGCTTG TACTCGGTCA TGGTTGTGGC CATATTATCA TCGTGTTTTT
  781 CAAAGGAAAA CCACGTCCCC GTGGTTCGGG GGGCCTAGAC GTTTTTTTTA ACCTCGACTA
  841 AACACATGTA AAGCATGTGC ACCGAGGCCC CAGATCAGAT CCCATACAAT GGGGTACCTT
  901 CTGGGGCATC CTTCAGCCCC TTGTTGAATA CGCTTGAGGA GAGCCATTTG ACTCTTTCCA
  961 CAACTATCCC AACTCACAAC GTGGCACTGG GGGTTGTGCC CGCCCTTTGC AGGTGTATCT
 1021 TTATACACGT GGGCTTTTTG GGCCGGCAGA GCAACCTGTC GCCAGGTGGG GGGGTTCGGC
 1081 TGCCTGCAAA GGGGTTCGCT ACAGACGTGA TTTGTCTCCA AGGAAAGCCT TCCAAGAAGG
1141 AACTGTGCCC T
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

2019.03.11

GNP_filter3_RDBDEP_html_190307.pl