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pCMFlag_hsNFKB1 (#RDB07018)

Expression vector of human NFKB1.

Alternative name SET-0132_16
Clone info. Expression vector of human NFKB1. Expression has not yet been confirmed.
Comment PCR cloning, forward primer: gcagaagatgatccatatttgggaag; reverse primer: ctaaattttgccttctagaggtccttc. Nucleotide substitution T2603G (silent), G2222A (silent) to NM_003998.2.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human NFKB1 cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB07018.pdf

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RDB07018 pCMFlag_hsNFKB1 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsNFKB1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07018).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08125_A5Af.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB08125_A5Afa.seq check
>07018_08125_A5Af_CMV-Forward_G08_20.ab1
    1 ATTTCACCTC CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGCCC GGAGGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGAA TTCACCATGG ACTACAAGGA CGATGACGAT
  181 AAGGCGGCCG CAGAAGATGA TCCATATTTG GGAAGGCCTG AACAAATGTT TCATTTGGAT
  241 CCTTCTTTGA CTCATACAAT ATTTAATCCA GAAGTATTTC AACCACAGAT GGCACTGCCA
  301 ACAGCAGATG GCCCATACCT TCAAATATTA GAGCAACCTA AACAGAGAGG ATTTCGTTTC
  361 CGTTATGTAT GTGAAGGCCC ATCCCATGGT GGACTACCTG GTGCCTCTAG TGAAAAGAAC
  421 AAGAAGTCTT ACCCTCAGGT CAAAATCTGC AACTATGTGG GACCAGCAAA GGTTATTGTT
  481 CAGTTGGTCA CAAATGGAAA AAATATCCAC CTGCATGCCC ACAGCCTGGT GGGAAAACAC
  541 TGTGAGGATG GGATCTGCAC TGTAACTGCT GGACCCAAGG ACATGGTGGT CGGCTTCGCA
  601 AACCTGGGTA TACTTCATGT GACAAAGAAA AAAGTATTTG AAACACTGGA AGCACGAATG
  661 ACAGAGGCGT GTATAAGGGG CTATAATCCT GGACTCTTGG TGCACCCTGA CCTTGCCTAT
  721 TTGCAAGCAG AAGGTGGAGG GGACCGGCAG CTGGGAGATC GGGAAAAAGA GCTAATCCGC
  781 CAAGCAGCTC TGCAGCAGAC CAAGGAGATG GACCTCAGCG TGGTGCGGCT CATGTTTACA
  841 GCTTTTCTTC CGGATAGCAC TGGCAGCTTC ACAAGGCGCC TGGAACCCGT GGTATCAGAC
  901 GCCATCTATG ACAGTAAAGC CCCCCAATGC ATCCAACTTG AAAATTTGTA AGAATGGGAC
  961 AGGGACAGCT GGATGTGTGA CTGGGAGGGG AGGGAAATTT ATCTTCTTTT GTGACAAAGT
 1021 TCAGAAAGAT GGACATCCAG ATTTCGATTT TTATGAAAGA AGAGAAATTG ATGTAGTCTG
1081 AAAGGATTGC AGATTTTTCC CTCCACAAGA ATGGTCATAG CAACTAAAT
//
Primer: BGH_rev
Sequence file: RDB08125_A5Afb.seq check
>07018_08125_A5Af_BGHrev_H08_23.ab1
    1 GGCTGCATTT AGGTGACTAT AGAATAGGGC CCTCTAGATG CATGCTCGAG CGGCCCTAAA
   61 TTTTGCCTTC TAGAGGTCCT TCCTGCCCAT AATCATGGGG CATTTTGTTG AGAGTTAGCA
  121 GTGAGGCACC ACTGGTCAGA GACTCGGTAA AGCTGAGTTT GCGGAAGGAT GTCTCCACGC
  181 CGCTGTCGCA GACACTGTCA CTGTCTCGGA GCTCGTCTAT TTGCTGCCTT GTGGAGGCAG
  241 GCGAGAGAGG CAGCGAGTGG GCCTGAGAGG TGGTCTTCAC TGGGCTGGAG GCTGCCTGGA
  301 TCACTTCAAT TGCTTCGGTG TAGCCCATTT GTCTCAGGGC CTCCACCAGC TCTCTGACTG
  361 TACCCCCAGA GACCTCATAG TTGTCCATAA GTGTTTTGGA AGGAGCAGGA CTCAGCCGGA
  421 AGGCATTATT AAGTATCCCC AGACCTAATT TCTGCGCCAG AGTAGCCCAG TTTTTGTCTG
  481 GATCAGGAAT TTCTAGTAAC TTATACAGCT GCAGCTTCAC ATCTTCAGCC AGCTGTTTCA
  541 TGTCTCCTTG TGCTAGTAAA TCATCAGATG TAAACTCTGG CTCATATGGT TTCCCATTTA
  601 ATATGTCAAA TACCTGCCAG CTGGTGGCCA TATCTAGAGG CGTGGTTCCA GGCACAACTC
  661 CTTCATCCTC TCCTGCATTT TCCCAAGAGT CATCCAGGTC ATAGAGAGGC TCAAAGTTCT
  721 CCACCAGGGG ATCTGCTCCT GCTGCTTTGA GAAGAGCTGC CAGCCTGGTG GACCCTCTCC
  781 CAGCTGCTAT ATGCAGGGGT GTGGTTCCAT CGTAGGTAGT ACTGTCCACG TGGGCATCAC
  841 CCTCCAGGAG CAGGCAGCCT GCCAATGAGA TGTTGTCGTG CTCCACAGCC AGGTGCAGTG
  901 CTGTGCGCCC GGACTTCTGC TCCTGAGCAT TGACGTCAGC CCCAGCGGCC CACCAGCAGC
  961 AGCAAACATG GGCAGGCTAT TGCTCATCAT GGCTAGATGA ATGGCATTTC AGACCGGTCC
 1021 CCGTTGGGGT GATCAGAAGT TAGTTGCCTG CTTTTTGTTG CTTTGGAGGT AGATACTGAG
1081 ACTTTAATCA TGGATCGTTC GTTGGCCAAG CA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles