RDB07006 - pCMFlag

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pCMFlag_hsIL2RG (#RDB07006)

Expression vector of human IL2RG.

Alternative name	SET-0197_4
Clone info.	Expression vector of human IL2RG. Expression has not yet been confirmed.
Comment	Derived from Sugano TMS05755. PCR cloning, forward primer: atgttgaagccatcattaccattc; reverse primer: ttcaggtttcaggctttaggg.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human IL2RG/IL-2R gamma cDNA
Depositor\|Developer	DNA Bank, \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
Additional terms and conditions:
Regrding pRc/CMV:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
付加的使用条件：
pRc/CMVについて:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

Catalog #	Resource name	Shipping form	Fee
RDB07006	pCMFlag_hsIL2RG	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsIL2RG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07006).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13004_A4Jv.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13004_A4Jva.seq
>07006_13004_A4Jv_CMV-Forward_E07_13.ab1 1 CTTCCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG 181 CGGCCATGTT GAAGCCATCA TTACCATTCA CATCCCTCTT ATTCCTGCAG CTGCCCCTGC 241 TGGGAGTGGG GCTGAACACG ACAATTCTGA CGCCCAATGG GAATGAAGAC ACCACAGCTG 301 ATTTCTTCCT GACCACTATG CCCACTGACT CCCTCAGTGT TTCCACTCTG CCCCTCCCAG 361 AGGTTCAGTG TTTTGTGTTC AATGTCGAGT ACATGAATTG CACTTGGAAC AGCAGCTCTG 421 AGCCCCAGCC TACCAACCTC ACTCTGCATT ATTGGTACAA GAACTCGGAT AATGATAAAG 481 TCCAGAAGTG CAGCCACTAT CTATTCTCTG AAGAAATCAC TTCTGGCTGT CAGTTGCAAA 541 AAAAGGAGAT CCACCTCTAC CAAACATTTG TTGTTCAGCT CCAGGACCCA CGGGAACCCA 601 GGAGACAGGC CACACAGATG CTAAAACTGC AGAATCTGGT GATCCCCTGG GCTCCAGAGA 661 ACCTAACACT TCACAAACTG AGTGAATCCC AGCTAGAACT GAACTGGAAC AACAGATTCT 721 TGAACCACTG TTTGGAGCAC TTGGTGCAGT ACCGGACTGA CTGGGACCAC AGCTGGACTG 781 AACAATCAGT GGATTATAGA CATAAGTTCT CCTTGCCTAG TGTGGATGGG CAGAAACGCT 841 ACACGTTTCG TGTTCGGAGC CGCTTTAACC CACTCTGTGG AAGTGCTCAG CATTGGAGTG 901 AATGGAGCCA CCCAATCCAC TGGGGGGAGC AATACTTCAA AAGAGAATCC TTTCCTGTTT 961 GCATTGGAAA GCCGTGGTTA TCTCTGTTGG CTCCATGGGA TTGATTATCA GCCTTTCTCT 1021 GTGTGTATTT CTGGCTGGGA ACGGAACGAT GCCCCGAATT TCCCCACCCT GAAGATTAGA 1081 GGAATCTTGT TACTGATACC ACGGACCTTT TCCACTGCAG TTGATGGTGT CTAACGGACG 1141 TTGGGCTTGG AGAAGTCCCT GC //
Primer: BGH_rev Sequence file: RDB13004_A4Jvb.seq
>07006_13004_A4Jv_BGH_rev_F07_16.ab1 1 GCTGGCATTA GGTGCCTATA GAATAGGGCC CTCTAGATGC ATGCTCGAGC GGCCTTCAGG 61 TTTCAGGCTT TAGGGTGTAA CATGGGGGGG CCCAGTAGGG GCTATGCTGG TTGCATGGGG 121 AGGCCCCAGG CCCCTCCCCA AGGGCCCCTC CTTTTGGGGG AATCTCACTG ACGAGGCAGA 181 GTCGTTCACT GTAGTCTGGC TGCAGACTCT CAGCCAGTCC CTTAGACACA CCACTCCAGG 241 CCGAAAAGTT CCCGTGGTAT TCAGTAACAA GATCCTCTAG GTTCTTCAGG GTGGGAATTC 301 GGGGCATCGT CCGTTCCAGC CAGAAATACA CACAGAGAAG GCTGATAATC AATCCCATGG 361 AGCCAACAGA GATAACCACG GCTTCCAATG CAAACAGGAA AGGATTCTCT TTTGAAGTAT 421 TGCTCCCCCA GTGGATTGGG TGGCTCCATT CACTCCAATG CTGAGCACTT CCACAGAGTG 481 GGTTAAAGCG GCTCCGAACA CGAAACGTGT AGCGTTTCTG CCCATCCACA CTAGGCAAGG 541 AGAACTTATG TCTATAATCC ACTGATTGTT CAGTCCAGCT GTGGTCCCAG TCAGTCCGGT 601 ACTGCACCAA GTGCTCCAAA CAGTGGTTCA AGAATCTGTT GTTCCAGTTC AGTTCTAGCT 661 GGGATTCACT CAGTTTGTGA AGTGTTAGGT TCTCTGGAGC CCAGGGGATC ACCAGATTCT 721 GCAGTTTTAG CATCTGTGTG GCCTGTCTCC TGGGTTCCCG TGGGTCCTGG AGCTGAACAA 781 CAAATGTTTG GTAGAGGTGG ATCTCCTTTT TTTGCAACTG ACAGCCAGAA GTGATTTCTT 841 CAGAGAATAG ATAGTGGCTG CACTTCTGGA CTTTATCATT ATCCGAGTTC TTGTACCAAT 901 AATGCAGAGT GAGGTTGGTA GGCTGGGGCT CAGAGCTGCT GTTCCAAGTG CAATTCATGT 961 ACTCGACATT GAACACAAAA CACTGAACCC TCTGGGAGGG GCAGAGTGGA AAACACTGAG 1021 GGAGTCAGTG GGGCATAGTG GTCAGAGAAT CAGCTGTGGT GTCTCATCCC ATTGGGCGTC 1081 AGAATTGTCG TGATCAGCCC AACTCCCAGG CAGGGCCAGC CTGCAAGCAT TAAGAGGGCA 1141 TGGTGTGCAT //