Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsSMAD2 (#RDB07000)

Expression vector of human SMAD2.

Alternative name	SET-0209_1-1
Clone info.	Expression vector of human SMAD2. Expression has not yet been confirmed.
Comment	Derived from Sugano CAS09876. PCR cloning, forward primer: atgtcgtccatcttgccattcacg; reverse primer: ttatgacatgcttgagcaacgcact. cDNA fragment 293 to 1696nt of NM_005901.5 (cds 293-1696nt) was cloned. Known difference: T to C substitution at 583nt (silent).
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human MADH2/SMAD2 cDNA
Depositor|Developer	DNA Bank, |

 

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB07000	pCMFlag_hsSMAD2	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsSMAD2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07000).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14573_A7B6p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14573_A7B6a.seq
>07000_14573_A7B6_CMV-Forward_A11_02_ABI24.ab1     1 CTGTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG   181 CGGCCATGTC GTCCATCTTG CCATTCACGC CGCCAGTTGT GAAGAGACTG CTGGGATGGA   241 AGAAGTCAGC TGGTGGGTCT GGAGGAGCAG GCGGAGGAGA GCAGAATGGG CAGGAAGAAA   301 AGTGGTGTGA GAAAGCAGTG AAAAGTCTGG TGAAGAAGCT AAAGAAAACA GGACGATTAG   361 ATGAGCTTGA GAAAGCCATC ACCACTCAAA ACTGTAATAC TAAATGTGTT ACCATACCAA   421 GCACTTGCTC TGAAATTTGG GGACTGAGTA CACCAAATAC GATAGATCAG TGGGACACAA   481 CAGGCCTTTA CAGCTTCTCT GAACAAACCA GGTCTCTTGA TGGTCGTCTC CAGGTATCCC   541 ATCGAAAAGG ATTGCCACAT GTTATATATT GCCGATTATG GCGCTGGCCT GATCTTCACA   601 GTCATCATGA ACTCAAGGCA ATTGAAAACT GCGAATATGC TTTTAATCTT AAAAAGGATG   661 AAGTATGTGT AAACCCTTAC CACTATCAGA GAGTTGAGAC ACCAGTTTTG CCTCCAGTAT   721 TAGTGCCCCG ACACACCGAG ATCCTAACAG AACTTCCGCC TCTGGATGAC TATACTCACT   781 CCATTCCAGA AAACACTAAC TTCCCAGCAG GAATTGAGCC ACAGAGTAAT TATATTCCAG   841 AAACGCCACC TCCTGGATAT ATCAGTGAAG ATGGAGAAAC AAGTGACCAA CAGTTGAATC   901 AAAGTATGGA CACAGGCTCT CCAGCAGAAC TATCTCCTAC TACTCTTTCC CCTGTTAATC   961 ATAGCTTGGA TTTACAGCCC AGTTTACTTA CTCAGACCTG CATTTTGGTG TTCGATAGCA  1021 TATTATGATT TAAATCAGAG GTTGGGAGAA ACCTTTCATG CATCACAGCC CTCCACTTCA 1081 CTGTAGATTG CCTTTACGGA CCATTCCAAA ATTCAAGAAG AAAGGATTC //
Primer: BGH_rev2 Sequence file: RDB14573_A7B6b.seq
>07000_14573_A7B6_BGH_rev2_B11_05_ABI24.ab1     1 CGTTTCGCGC TCAGGACGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC    61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT   121 AGATGCATGC TCGAGCGGCC TTATGACATG CTTGAGCAAC GCACTGAAGG GGATCCCATC   181 TGAGTTAATA CTTTGTCCAA CCACTGTAGA GGTCCATTCA GATGAAGTTC AATCCAGCAA   241 GGAGTACTTG TTACCGTCTG CCTTCGGTAT TCTGCTCCCC ACCCTTTCAC AAAACTCATT   301 CTTATGGTGC ACATTCTAGT TAGCTGATAG ACGGCTTCAA AACCCTGATT AACAGACTGA   361 GCCAGAAGAG CAGCAAATTC CTGGTTGTTG AAGATCTTCA GATTACAGCC TGGTGGAATT   421 TTACACACTG TTGCAGGGTG CCAGCCATAT CTCTGATTAC AATTGGGGCT CTGCACAAAG   481 ATTGCACTAT CACTTAGGCA CTCAGCAAAA ACTTCCCCAC CTATGTAGTA TAAGCGCACT   541 CCTCTTCCTA TATGCCTTCT TGTCATTTCT ACCGTGGCAT TTCGGTTAAC ATTGGAGAGT   601 AAACCTAAGC AGAACCTCTC TGAATTTGAT GGGTCTGTAA AGCCATCTAC AGTGAGTGAG   661 GGCTGTGATG CATGGAAGGT TTCTCCAACC CTCTGATTTA ATTCATAATA TGCTATCGAA   721 CACCAAAATG CAGGTTCTGA GTAAGTAACT GGCTGTAAAT CCAAGCTATG ATTAACAGGG   781 GAAAGAGTAG TAGGAGATAG TTCTGCTGGA GAGCCTGTGT CCATACTTTG ATTCAACTGT   841 TGGTCACTTG TTTCTCCATC TTCACTGATA TATCCAGGAG GTGGCGTTTC TGGAATATAA   901 TTACTCTGTG GCTCAATTTC CTGCTGGGAA GTTAGTGTTT TTCTGGAATG GGAGTGAGTA   961 TAGTCATCCA GAGGCCGGAA GTTTCTGTTA GAATCTCCGG TGTGTCGGGG CACTTAATAC  1021 TGGAGGCAAA ACTTGCTGTC CTCACTCCTC TGATAGTGGG TTAGGGCTTA CGCATACCGT  1081 TCAATCCCTT TTTTAGGATG AAAGCACATA TCGCCCAGGC TTTTCCAGAG TTGCCTTTGT 1141 GAGG //
Primer: SV40pro_F_V2 Sequence file: RDB14573_A7B6c.seq
>07000_14573-A7B6_SV40proF_V2_E04_13_ABI24.ab1     1 GGACCAAGCT TTTTTGGAGG GCTAGGCTTT TGCAAAAGCT CCCGGGAGCT TGGATATCCA    61 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT   121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC   181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC   241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC   301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG   361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC   421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG   481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC   541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC   601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA   661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA   721 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG   781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG   841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG   901 GACTCTGGGG GTTCGAAATG ACCGACCCAA GCGACGCCCA ACCCTGCCCA TCACGAGATT   961 TCGATTCCAC CGCCCGCCTT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGACGCC  1021 GGCTGGATGA TCCTCAGCGC GGGATCTCAT GCCTGAGTCC TCGGCCAACC CCAACTGTAA 1081 TGGCAGCTTA ATATGTACAA ATTAAGGCAA TATAAGGC //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Other resources in our bank

• human MADH2/SMAD2

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External Database
Human MADH2/SMAD2

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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