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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsIL6

Expression vector of human IL6.

Catalog number RDB06998
Resource name pCMFlag_hsIL6
Alternative name SET-0199_12-1
Clone info. Expression vector of human IL6. Expression has not yet been confirmed.
Comment Derived from Sugano CAS04362. PCR cloning, forward primer: atgaactccttctccacaagc; reverse primer: ctacatttgccgaagagccctc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human IL6 cDNA
Depositor|Developer DNA Bank, |
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External Database
Human IL6

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06998 pCMFlag_hsIL6 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06998.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12822_A4Ggp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12822_A4Gga_2.seq check
          >06998_12822_A4Gg_CMV-Foward_A08_02.ab1
              1 CTTAACAACC TTTCCGCCCC CATTTGACCG CAAATGGGCG GTAGGCGGTG TACGGTGGGA
             61 GGTCTTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
            121 AAATTAATAC GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG
            181 ATGACGATAA GGCGGCCATG AACTCCTTCT CCACAAGCGC CTTCGGTCCA GTTGCCTTCT
            241 CCCTGGGGCT GCTCCTGGTG TTGCCTGCTG CCTTCCCTGC CCCAGTACCC CCAGGAGAAG
            301 ATTCCAAAGA TGTAGCCGCC CCACACAGAC AGCCACTCAC CTCTTCAGAA CGAATTGACA
            361 AACAAATTCG GTACATCCTC GACGGCATCT CAGCCCTGAG AAAGGAGACA TGTAACAAGA
            421 GTAACATGTG TGAAAGCAGC AAAGAGGCAC TGGCAGAAAA CAACCTGAAC CTTCCAAAGA
            481 TGGCTGAAAA AGATGGATGC TTCCAATCTG GATTCAATGA GGAGACTTGC CTGGTGAAAA
            541 TCATCACTGG TCTTTTGGAG TTTGAGGTAT ACCTAGAGTA CCTCCAGAAC AGATTTGAGA
            601 GTAGTGAGGA ACAAGCCAGA GCTGTGCAGA TGAGTACAAA AGTCCTGATC CAGTTCCTGC
            661 AGAAAAAGGC AAAGAATCTA GATGCAATAA CCACCCCTGA CCCAACCACA AAATGCCAGC
            721 CTGCTGACGA AGCTGCAGGC ACAGAACCAG TGGCTGCAGG ACATGACAAC TCATCTCATT
            781 CTGCGCAGCT TTAAAGGAGT TCCTGCAGTC CAGCCTGAGG GCTCTTCGGC AAAATGTAGG
            841 GCCCGCTCGA GCATGCATCT AGAGGGCCCT ATTCTATAGT GTCACCCTAA AATGCTAAAG
            901 CTCGCTGATC AGCCCTCGAA CTGTGCCTTC TAATTTGCCC AGCCCATCTG TTGTTTGCCC
            961 CTCCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCAACTC CCCACTTGTC CTTTTCCTAA
           1021 TAAAATGAAG GAAAATTGCA TCCGCATTTG TCCTGAGTAG GTGTCCATTT CTATTCTGGG
           1081 GGGTTGGGGC TGGGGGGCAA GGACACAAGC CAATGGGGGG AAAGATCTGG GGAAAAAAAC
           1141 AAAATAACCA AGGCATTGCT TGGGTAATGC CGGGTGGGCC TTCTAATTGC CCTTCCTGTG
          1201 AGAGGCCGTA AAGAACCAGA CTTGGCGACT CTTGCGATAG AAGGTGGTGG TAGC
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB12822_A4Ggb_2.seq check
          >06998_12822_A4Gg_SV40pro_F_V2_E01_13.ab1
              1 TAGACTTTTG GAGCTAGGCT TTTGCAAAAG CTCCCGGGAG CTTGGATATC CATTTTCGGA
             61 TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA
            121 GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC
            181 GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC
            241 AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG
            301 CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG
            361 GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT
            421 GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT
            481 ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA
            541 GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA
            601 CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC
            661 GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT
            721 GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT
            781 GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC
            841 GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG
            901 GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC
            961 GCCCGCCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT TCCCGGGACG CCCGGCTGGG
           1021 ATGATTCCTT CCCAGCGCGG GGGATCTCAT GCTGGGAGTT TCATCACCAC CCCGACCTTG
          1081 CTTTATTGCA GGCATAATAA ATG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Yokoyama, C., Interleukin-8 enhances the effect of colchicine on cell death. Biochem. Biophys. Res. Commun. 485 (1): 89-94 (2017). PMID 28189686.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl