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pEF-BOSneo-Myc-MINT (1-3576)-FLAG

Expression vector of mouse SPEN/MINT cDNA

Catalog number RDB06777
Resource name pEF-BOSneo-Myc-MINT (1-3576)-FLAG
Alternative name #11577
Clone info. Expression vector of mouse SPEN/MINT cDNA. FL in Figure 1A of Kuroda, K. et al., Immunity 18 (2), 301-312, 2003.
This clone possibly contains nucleotide substitutions comparing with NM_019763.2. See comment of RDB13473 and RDB13474.
Vector backbone pEF-BOS Neo SE (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Mint cDNA
Depositor Honjo, Tasuku |
 
Other clones in our bank mouse Spen (NCBI Gene 56381) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (Kuroda, K., Immunity, 18, 301-312, 2003) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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RDB06777 pEF-BOSneo-Myc-MINT (1-3576)-FLAG DNA solution

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References and tips

Electronic file

Featured content

References

original Kuroda, K., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13107_A5B3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB13107_A5B3a.seq
>06777_13107_A5B3_pAxEF_F1_E01_13.ab1
    1 CTTTTTTCGT TCATTTCAGG TGTCGTGAGG AATTCTCTAG CCTCGACGCC ACCATGGAGC
   61 AAAAGCTCAT TTCTGAAGAG GACTTGGGAG ACCGAGACCT CCGCACGGAT TATAATGAGC
  121 CAGGAACTAT TCCGAGTGCT GCCCGGGGAC TGGACGAGAC AGTTTCCATA GCGTCTCGTA
  181 GTAGAGAGGT TTCTGGGTTC AGAGGAAGTG CTGGAGGGCC TGCTTATGGT CCCCCACCAT
  241 CACTTCATGC ACGAGAAGGA CGCTATGAGC GGAGACTCGA TGGGGCTTCA GATAACAGGG
  301 AGCGTGCTTA TGAACATAGT GCCTATGGAC ACCATGAACG GGGGACAGGA GCATTTGATC
  361 GGACAAGACA TTACGATCAG GATTACTATA GAGACCCTCG AGAGCGGACT TTACAACACG
  421 GGCTCTATTA CACTTCTCGG AGTCGAAGTC CAAACCGCTT TGATGCTCAT GACCCCCGAT
  481 ACGAACCTAG GGCCCGGGAG CAGTTTACAC TGCCCAGTGT GGTACACAGG GATATCTACA
  541 GGGACGACAT TACCCGGGAG GTACGAGGCA GAAGGCCAGA GCGCAGTTAC CAACACAGCA
  601 GGAGTCGCTC ACCACATTCG TCCCAGTCTA GAAACCAGTC CCCGCAGAGG CTGGCTAGCC
  661 AAGCGTCCAG ACCCACCAGG TCCCCCAGCG GCAGCGGCTC CAGGAGTCGA TCCTCCAGTA
  721 GTGACTCCAT CAGCAGCAGC AGCAGCAGCA GCAACACTGA CAGTAGCGAC TCCAGCAGCA
  781 CGGCCAGTGA CGACTCTCCG GCCCGCTCTG TTCAGTCGGC TGCAGTCCCC GCACCCACTT
  841 CCCAGTTGCT TTCGTCCCTT GAAAAAGATG AGCCCCGGAA AAGTTTTGGG ATCAAAGTTC
  901 AGAATCTTCC AGTACGCTCT ACAGACACAA GCCTTAAAGA CGGCCTTTTC CATGAGTTTA
  961 AGAAGTTTGG CAAGGTGACG TCAGTGCAGA TCCACGGGGG CCCTCCGAGG GAGAGATACG
 1021 GGCCTGGTGT TTCTTTCCGG GCAGCAAGAG GGACCCAAGA AAAAGCATTT GACCCGCATC
 1081 CCGAGGGGGA AGCTCTTCTT TCGGGCCATG GCAGATCGAG TTGAACTTGC CAATGGGTTA
1141 GAACCAAGCA AACAGGAGGA GTGTGAAAAA TGTGAGAGTT TTTTTCG
//
Primer: hGCSF-R RDB13107_A5B3b.seq
>06777_13107_A5B3_hGCSF-R_F01_16.ab1
    1 CTGGAACTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGGATAT CATCTAGACG
   61 GATCCGCAAT TGGCCCGGCC GCTCTAGACT ACTTGTCATC GTCGTCCTTG TAATCCACCG
  121 AGGCAATGAC AATCATGAGG TGAGGAGAGA TGTTGGAGAT GCTGGCAAGG AGGTCAGGGG
  181 CCAGCCGGGA GAGGTGACTC TCGGAGAACT CACAGGGTGG GAAGATCTGA AGCACGTAGG
  241 CAGGCTGATT GGAGCCAGGG TTGGGGACAT TGATAATCCC TGCTGCTTGC TTGGCCTGCA
  301 GGTAAGTGAT GAAGGCAGCC TTGAGGGACT CGGTCTGGCT CACCACATCC TCCTGGTCAC
  361 GGCCACAGGG CAGAGCCAGC AGCAGGCAGT AGTCGGTTTC CACTGTCATT CTCCTGGCGA
  421 CCCCTTCCAG CTGTGAGGCC TCTAGTCGCA TCCTCTGGGC GATCCGCAGT GGGGGACCAC
  481 CTTCAGAGAG AGGCAGGGAC CGATGAGCCA GGACATTGTT GCCAGAGACG AAGTGGAGCT
  541 GCACAGCAGC CGTGTCGTTC TTGAGGGCCA GCAGGCCCTG CCACACGATG GGGTACTTCT
  601 TCAGCAGCTG GACCATATCC ACAGGCCGTT GTGGTGTCCC GTGTGGAGAA GACTCTTGTT
  661 TGGGAGCTGG CTGGACTTCA GCGTGAGGCA GAGCCAGTCC AGGTGGGGTG CTTGGGCCTG
  721 AGTTTGCAGG GACAAACAAT GGCTGCTTTG GGGCAGCCTG AGAGGAAAGA GGGCTTGGGA
  781 GGTCTGGCTT CATGGAGACG GACACAGGGG AGGGGTGGGC TGGCTGGGCT GTTTCCACCG
  841 GCGCCCTCTG AAGCTGAGCG TGTGGCTCAG ATGGCCTGTT TGTGGGGATG GCTGGGAGGC
  901 GAGTCTGCTC CACCCCTCCT GTCTGCGTCC CCTTTGCTTC TTGGGAGACC TGAGGAATCT
  961 TGCCACTCGG TGGCTGGGCA GGCTGGCTGA GTTGGGAGGG CTGGCAGGGC GGGGCAGGTG
 1021 GTATAGGCTG CGTGGGCCTG CGTAGAGGGG GCAGGCTGTG CAGACTGCGT GGACTGCAAA
 1081 GGCTCACCCT TCAGGGGCAC TGAGCCAGAA GTTCTTTGGA TCCTGGAAGG CCTTTAAGGC
1141 TGTGAATGTG GGAAAGCGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kuroda, K., Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway Immunity, 18, 301-312 (2003). PMID 12594956.

2019.02.05

GNP_filter3_RDBDEP_html_190131.pl