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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-BOSneo-mNotch4 RAMIC (#RDB06774)

Expression vector of mouse Notch4 intracellular region

Alternative name #9938
Clone info. Expression vector of mouse Notch4 intracellular region.
Comment This clone contains 729 bp sequence of pGEX 4T1 between the end of poly(A) stretch of mNotch4-RAM and polyadenylation sequence of the pEF-BOS neo since this clone was constructed on the pGEX 4T1 vector first and then a enzyme digested fragment was cloned into the pEF-BOS neo vector as mentioned in Kato et al., FEBS Lett. 395 (2-3): 221-224, 1996. (Aug. 08, 2014. T.M.)
Vector backbone pEF-BOS neo (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Notch gene homolog 4 cDNA
Depositor|Developer Honjo, Tasuku |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by DEPOSITOR (FEBS Lett., 395, 221-224, 1996) and an acknowledgment to Depositor are requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Remarks

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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。利用者は、研究成果の公表にあたって寄託者の指定する文献(FEBS Lett., 395, 221-224, 1996)を引用し、寄託者への謝辞の表明を必要とする。理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06774 pEF-BOSneo-mNotch4 RAMIC DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-BOSneo-mNotch4 RAMIC was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06774).

Reference section:

Kato, H., Sakai, T., Tamura, K., Minoguchi, S., Shirayoshi, Y., Hamada, Y., Tsujimoto, Y., Honjo, T., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12828_A4Gi.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEFF1
Sequence file: RDB12828_A4Gia.seq check
>06774_12828_A4Gi_pAxEF_F1_D10_10.ab1
    1 AAATTTTTTC TTCAATTTCA GGTGTCGTGA GGAATTCTCT AGAACACCAT GGAGCAAAAG
   61 CTCATTTCTG AAGAGGACTT GAGAGAATTC CAGCTCATTC GGCGACGGCG ACGAGAACAT
  121 GGGGCCCTGT GGCTGCCCCC TGGTTTCATT CGAAGGCCTC AGACACAGCA GGCACCCCAC
  181 CGGCGGAGGC CCCCACTGGG CGAGGACAAC ATTGGTCTTA AGGCACTGAA GCCAGAGGCC
  241 GAAGTGGATG AGGATGGAGT GGCCATGTGC TCGGGCCCTG AAGAGGGAGA GGCTGAAGAA
  301 ACAGCCTCAG CCTCCAGGTG CCAGCTTTGG CCGCTCAACA GCGGCTGTGG AGAGCTCCCC
  361 CAGGCAGCCA TGCTGACCCC TCCTCAGGAG TGTGAATCGG AGGTTCTGGA TGTGGACACC
  421 TGTGGACCTG ATGGGGTGAC ACCCCTGATG TCAGCCGTCT TCTGTGGGGG AGTGCAGTCC
  481 ACGACTGGGG CTAGTCCACA GAGACTGGGG CTAGGAAATC TGGAACCCTG GGAACCACTG
  541 CTGGATAGAG GGGCCTGCCC CCAGGCTCAC ACTGTGGGCA CTGGAGAGAC GCCTCTGCAC
  601 CTAGCTGCCA GATTCTCTCG GCCAACCGCT GCCCGCCGCC TCCTTGAGGC TGGAGCCAAC
  661 CCCAACCAGC CAGACCGCGC TGGGCGCACC CCACTTCACA CTGCTGTGGC TGCCGACGCT
  721 CGGGAGGTTT GCCAGCTCCT ATTGGCCAGC AGACAGACTA CGGTGGACGC CCGCACAGAG
  781 GACGGGACTA CACCTTTGAT GCTGGCTGCC AGGCTGGCCG TGGAGGACCT GGTTGAAGAA
  841 TTGATCGCAG CCCGAGCAGA TGTAGGAGCC AGGGATAAAA GGGGAAAAAC TGCACTGCAC
  901 TGGGCCGCTG CTGTGAACAA CGCCCGAGCC GCCCGCTCTC TCCTCCAGGC TGGAGCGGAT
  961 AAAGATGCCC AGGACAGTAG GGAACAGACG CCGCTTTTCC TGCAGCGCGC GAAAGGAGCC
 1021 GTGGAGGTGG CGCAGCTGTT TGCTGGAAGC TCGGGGCACC GGGACTGCGA GAACCAGGCC
 1081 GGCTGGCCCA GAGATGTGGT CCGGCAGCGC AGTTCACTTG GACTGCCTTA ACGCTGCCTG
1141 AAGGGGCTTG GAACCGAACC TAACG
//
Primer: hGCSF-R
Sequence file: RDB12828_A4Gib.seq check
>06774_12828_A4Gi_hGCSF_R_E10_13.ab1
    1 CTGGGGGGAC TGATCTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGAC TCAACCAAGT
   61 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ACACGGGATA
  121 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
  181 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
  241 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
  301 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
  361 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
  421 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  481 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  541 CGAGGCCCTT TCGTCTTCAA GAATTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG
  601 CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG
  661 CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC
  721 CGTCATCACC GAAACGCGCG AGGCAGATCG TCAGTCAGTC ACGATGCGGC CGCTCGAGTT
  781 TTTTTTTTTT TTTTTTTAGC ACTTCAATTA GCTTTATTAT CCATGAGTTT GGGGTTCTCG
  841 GAGATAGCAG TGACTGGAGA CATGGCCTCA TCCCTGCACC AGTGTCCTTG TGGGGAGGCA
  901 GAGAGAGGGG CAAGGAGTCA TCAGCTGGAA TGGGCCCACT GGTTTACTTA CTGTTCATCT
  961 ACACAACCAC CCGGGCACAT CGTAGGTGCT TTAATAAATA GTTGCCAAGC TACTTTGTGG
 1021 GCAGTAGATA GCAGAAGCTC CTTTTGGGGA AACCAATTAC CACAGAAAAC AATTTTAACC
 1081 ACCCCCAAAT TCGCCCACCA GGTCTTGTTC CCAGAAAGTT AGGAATCTCG AATCAACTTT
1141 GGTCCTCTTG AAGCTGCCTA TTTTTCCCAA GGGAAAAATA TCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kato, H., Functional conservation of mouse Notch receptor family members. FEBS Lett., 395, 221-224 (1996). PMID 8898100. [link to RRC of NBRP]