Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsTP53 (#RDB06754)

Expression vector of human p53.

Alternative name	SET-0136_2-5
Clone info.	Expression vector of human p53. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	PCR cloning, forward primer: gaggagccgcagtcagatcc; reverse primer: tcagtctgagtcaggcccttc.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human tumor protein p53 cDNA
Depositor|Developer	DNA Bank, |

 

Remarks, protocol and/or map (pdf)	RDB06754.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB06754	pCMFlag_hsTP53	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsTP53 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06754).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13744_A5Lop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13744_A5Loa.seq
>06754_13744_A5Lo_CMV-Forward_A03_03.ab1     1 TAATCAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC   121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA   181 GGCGGCCGAG GAGCCGCAGT CAGATCCTAG CGTCGAGCCC CCTCTGAGTC AGGAAACATT   241 TTCAGACCTA TGGAAACTAC TTCCTGAAAA CAACGTTCTG TCCCCCTTGC CGTCCCAAGC   301 AATGGATGAT TTGATGCTGT CCCCGGACGA TATTGAACAA TGGTTCACTG AAGACCCAGG   361 TCCAGATGAA GCTCCCAGAA TGCCAGAGGC TGCTCCCCCC GTGGCCCCTG CACCAGCAGC   421 TCCTACACCG GCGGCCCCTG CACCAGCCCC CTCCTGGCCC CTGTCATCTT CTGTCCCTTC   481 CCAGAAAACC TACCAGGGCA GCTACGGTTT CCGTCTGGGC TTCTTGCATT CTGGGACAGC   541 CAAGTCTGTG ACTTGCACGT ACTCCCCTGC CCTCAACAAG ATGTTTTGCC AACTGGCCAA   601 GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG CCCGGCACCC GCGTCCGCGC   661 CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT GTGAGGCGCT GCCCCCACCA   721 TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG CATCTTATCC GAGTGGAAGG   781 AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT CGACATAGTG TGGTGGTGCC   841 CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC CACTACAACT ACATGTGTAA   901 CAGTTCCTGC ATGGGCGGCA TGAACCGGGA GGCCCATCCT CACCATCATC ACACTGGAAG   961 ACTCCAGTGG TAATCTACTG GGACGGAACA GCTTTGAGTG CGTGTTTGTG CCTTGTCCTG  1021 GAGAGACCGG GCGGCACAGA AGGAAGGAGA ATCTTCCGGC AAGAAAAGGG GAGCCTTCAC  1081 CACGGGAGCC TGCCTCCAAG GGAAGCACTT AAGACGGAGC CACTGCCCAC AACTCCAGCT 1141 TCCTCTCTCC CCAGCCAAGG AGGAAACACA CTTGGCATAT GAAGAAATAT AAT //
Primer: BGH_rev Sequence file: RDB13744_A5Lob.seq
>06754_13744_A5Lo_BGHrev_B03_06.ab1     1 GTCCTGCATT GGTGAACTAT AGAATAGGGC CCTCTAGATG CATGCTCGAG CGGCCTCAGT    61 CTGAGTCAGG CCCTTCTGTC TTGAACATGA GTTTTTTATG GCGGGAGGTA GACTGACCCT   121 TTTTGGACTT CAGGTGGCTG GAGTGAGCCC TGCTCCCCCC TGGCTCCTTC CCAGCCTGGG   181 CATCCTTGAG TTCCAAGGCC TCATTCAGCT CTCGGAACAT CTCGAAGCGC TCACGCCCAC   241 GGATCTGAAG GGTGAAATAT TCTCCATCCA GTGGTTTCTT CTTTGGCTGG GGAGAGGAGC   301 TGGTGTTGTT GGGCAGTGCT CGCTTAGTGC TCCCTGGGGG CAGCTCGTGG TGAGGCTCCC   361 CTTTCTTGCG GAGATTCTCT TCCTCTGTGC GCCGGTCTCT CCCAGGACAG GCACAAACAC   421 GCACCTCAAA GCTGTTCCGT CCCAGTAGAT TACCACTGGA GTCTTCCAGT GTGATGATGG   481 TGAGGATGGG CCTCCGGTTC ATGCCGCCCA TGCAGGAACT GTTACACATG TAGTTGTAGT   541 GGATGGTGGT ACAGTCAGAG CCAACCTCAG GCGGCTCATA GGGCACCACC ACACTATGTC   601 GAAAAGTGTT TCTGTCATCC AAATACTCCA CACGCAAATT TCCTTCCACT CGGATAAGAT   661 GCTGAGGAGG GGCCAGACCA TCGCTATCTG AGCAGCGCTC ATGGTGGGGG CAGCGCCTCA   721 CAACCTCCGT CATGTGCTGT GACTGCTTGT AGATGGCCAT GGCGCGGACG CGGGTGCCGG   781 GCGGGGGTGT GGAATCAACC CACAGCTGCA CAGGGCAGGT CTTGGCCAGT TGGCAAAACA   841 TCTTGTTGAG GGCAGGGGAG TACGTGCAAG TCACAGACTT GGCTGTCCCA GAATGCAAGA   901 AGCCCAGACG GAAACCGTAG CTGCCCTGGT AGGTTTTCTG GGAAAGGGAC AGAAGATGAC   961 AGGGGCCAGG AGGGGGCTGG GTGCAGGGCC CGCCGGGTGT AGAGCTGCTG GGTGCAGGGC  1021 CACGGGGGGG AGCAGCCTCT GGCATTCTGG GAGCTTCATC TGGGACCTGG GGTCTCAGTG  1081 ACCATGATCA ATATCGTCCG GGACGCATCC AATTCATTCA TGCTGGGACG GCAAGGGGGA 1141 ACAGAAACGG //
Primer: SV40pro_F_V2 Sequence file: RDB13744_A5Loc.seq
>06754_13744_A5Lo_SV40pro_F_V2_E12_15.ab1     1 GAACTTTTTT GGAGCTAGGC TTTTGCAAAA GCTCCCGGGA GCTTGGATAT CCATTTTCGG    61 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC   121 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT   181 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT   241 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG   301 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG   361 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC   421 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC   481 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA   541 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA   601 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG   661 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG   721 TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC   781 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTTCCTCGT GCTTTACGGT ATCGCCCGCT   841 CCCGATTCGC AGCGCATCGC CTTCTATCGC CCTTCTTGAC GAGTTCTTCT GAGCGGGACT   901 CTGGGGGTTC GAAATGACCG AACCAGCGAC GCCCAACCTG CATCACGAGA TTTCGATCAA   961 CGGCGCTTCC TATGAAAGGT GGCTCCGATC GTTTTCCGGA CGCGCTGATG ATCTCAGCGC 1021 GGCAATCTCA TGCTGAGAGG T //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

user_report	Shimizu, H., NF-kappaB plays an important role in indoxyl sulfate-induced cellular senescence, fibrotic gene expression, and inhibition of proliferation in proximal tubular cells. Am. J. Physiol. Cell Physiol. 301 (5): C1201-12 (2011). PMID 21832251. [link to RRC of NBRP]

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Other resources in our bank

• human tumor protein p53

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External Database
Human tumor protein p53

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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