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pN1-G-CaMP2 (#RDB06750)

Genetically-encoded fluorescent calcium sensor cDNA

Alternative name pN1-REST-mG1.6#X-1
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. Its fluorescence is brighter than that of G-CaMP1.6.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence DQ381402 (DDBJ accession number)
Depositor|Developer Nakai, Junichi |
 
Sequence RDB06750z.seq
Remarks, protocol and/or map (pdf) RDB06750.pdf

Distribution information

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RDB06750 pN1-G-CaMP2 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pN1-G-CaMP2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06750).

Reference section:

Tallini, Y.N., Ohkura, M., Choi, B.R., Ji, G., Imoto, K., Doran, R., Lee, J., Plan, P., Wilson, J., Xin, H.B., Sanbe, A., Gulick, J., Mathai, J., Robbins, J., Salama, G., Nakai, J., Kotlikoff, M.I., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14349_A6Iup1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14349_A6Iua.seq check
>06750_14349_A6Iu_CMV-Forward_A01_01_ABI24.ab1
    1 CGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGACTCAGAT CTCGCCACCA
  121 TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA
  181 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC
  241 GTCGTAAGTG GAATAAGACA GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACTCG
  301 AGAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGCGAAC TTCAAGATCC
  361 GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCTACCA CTACCAGCAG AACACCCCCA
  421 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCAAACTTT
  481 CGAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG
  541 GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC ATGGTGAGCA
  601 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  661 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GTGAGGGCGA TGCCACCTAC GGCAAGCTGA
  721 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  781 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
  841 TCTTCAAGTC CGCCATGCCC GAAAGGCTAC ATCCAGGAGC GCACCATCTT CTTCAAAGGA
  901 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGTGACCGCA
  961 TCGAGCTGAT GGCATCGACT CAGAGACGGC ACATCTGGGC ACAGCTGGAG TACACACGCG
 1021 TGACACTGAC TGGAGAGCCA GATCGCAGAG TCAGAGCCTT TCTCCGTATT GACATGACGG
1081 GCATGGACAT AACATCAACG AGCTGGGGAA CGCGA
//
Primer: ME-1290Rv
Sequence file: RDB14349_A6Iub.seq check
>06750_14349_A6Iu_ME-1290Rv_B01_04_ABI24.ab1
    1 ATGCAATTCA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
   61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTCACTTCG
  121 CTGTCATCAT TTGTACAAAC TCTTCGTAGT TTACCTGACC ATCCCCATCG ATGTCTGCTT
  181 CCCTGATCAT TTCATCAACC TCTTCATCTG TTAACTTCTC TCCAAGGTTT GTCATCACGT
  241 GGCGAAGCTC TGCTGCACTG ATGTAGCCAT TGCCATCCTT ATCAAACACA CGGAACGCTT
  301 CTCTAATTTC TTCTTCACTG TCTGTGTCTT TCATTTTTCT TGCCATCATT GTCAGGAACT
  361 CAGGGAAGTC GATTGTGCCA TTACCGTCGG CATCTACTTC ATTGATCATG TCCTGCAGCT
  421 CTGCTTCTGT GGGGTTCTGC CCCAGAGACC GCATCACCGT CCCCAGCTCC TTGGTTGTTA
  481 TTGTCCCATC CCCGTCCTTG TCAAATAGGG AGAAAGCCTC TTTAAATTCT GCGATCTGCT
  541 CTTCAGTCAG TTGGTCACGC GTGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT
  601 CCTTGAAGTC GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA
  661 CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGATGT
  721 AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC
  781 TGAAGCACTG CACGCCGTAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT
  841 TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCACCCT
  901 CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA
  961 CCACCCCGGT GAACAGCTCT CGCCCTTGCT CACATGCTCC CCTCGGGTAC GCCCTGTACA
 1021 GCTCGTCATG CCGAGAGTGA TCCTGCCGCC GTCACGAACT TCAAGCAAGA CATGTGATCG
1081 CGCATCTCGT AGGATCATCG CAGTTGACCT GGGGTGTCTC AAGGTAATG
//
Primer: SV40pro_F_V2
Sequence file: RDB14349_A6Iuc.seq check
>06750_14349_A6Iu_SV40pro_F_V2_C01_07_ABI24.ab1
    1 TAGGCGTTTT GGAGCTAGGC TTTTGCAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC
   61 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  121 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  181 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  241 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  301 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  361 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  421 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  481 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  541 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
  601 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  661 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
  721 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
  781 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
  841 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA
  901 CCCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTTCGGA
  961 ATCGTTTTCC CGGGGACGCC CGGCTGGATG ATCTCAGCGC GGGGATCTCA TGCTGAGTCT
 1021 CGCCCACCCT ACGGGGAAGC CTACCTGAAA CACGGCAGCA GACATACTGA GGACCCGCGC
1081 TATGAACGGC CAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tallini, Y.N., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386. [link to RRC of NBRP]
user_report Fujii, Y., Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels. Sci. Rep. 7 (1): 13115 (2017). PMID 29030562. [link to RRC of NBRP]