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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pN1-G-CaMP2

Genetically-encoded fluorescent calcium sensor cDNA

Catalog number RDB06750
Resource name pN1-G-CaMP2
Alternative name pN1-REST-mG1.6#X-1
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. Its fluorescence is brighter than that of G-CaMP1.6.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence DQ381402 (DDBJ accession number)
Depositor|Developer Nakai, Junichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Schedule A (GFP-A) [Link]
        Approval form by depositor [Word]
        Remarks ((Additional form)) Approval Form(FormD)
        Please use Schedule A (GFP-A) additionally.
        Please obtain written approval of the depositor.
        提供案内 (日本国内) [open/close]

        提供条件 寄託者に許可を取ること。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。研究成果の発表の時、謝辞に承諾者名を入れる。寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        Schedule A(GFP-A, 英語版のみ) [Link]
        寄託者による提供承諾書 [Word]
        備考 【追加提供依頼書】承諾書(FormD)
        Schedule A(GFP-A, 英語版のみ)が追加で必要です。
        寄託者の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB06750 pN1-G-CaMP2 DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Sequence RDB06750z.seq
        Remarks, protocol and/or map (pdf) RDB06750.pdf

        Original reference

        original Tallini, Y.N., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content GFP Resources (English text)
        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14349_A6Iup1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV-Forward
        Sequence file: RDB14349_A6Iua.seq check
        >06750_14349_A6Iu_CMV-Forward_A01_01_ABI24.ab1
            1 CGTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
           61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGCTACC GGACTCAGAT CTCGCCACCA
          121 TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA
          181 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC
          241 GTCGTAAGTG GAATAAGACA GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACTCG
          301 AGAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGCGAAC TTCAAGATCC
          361 GCCACAACAT CGAGGACGGC GGCGTGCAGC TCGCCTACCA CTACCAGCAG AACACCCCCA
          421 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCAAACTTT
          481 CGAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG
          541 GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC ATGGTGAGCA
          601 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
          661 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GTGAGGGCGA TGCCACCTAC GGCAAGCTGA
          721 CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
          781 CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
          841 TCTTCAAGTC CGCCATGCCC GAAAGGCTAC ATCCAGGAGC GCACCATCTT CTTCAAAGGA
          901 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGTGACCGCA
          961 TCGAGCTGAT GGCATCGACT CAGAGACGGC ACATCTGGGC ACAGCTGGAG TACACACGCG
         1021 TGACACTGAC TGGAGAGCCA GATCGCAGAG TCAGAGCCTT TCTCCGTATT GACATGACGG
        1081 GCATGGACAT AACATCAACG AGCTGGGGAA CGCGA
        //
        Primer: ME-1290Rv
        Sequence file: RDB14349_A6Iub.seq check
        >06750_14349_A6Iu_ME-1290Rv_B01_04_ABI24.ab1
            1 ATGCAATTCA ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG
           61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCTCACTTCG
          121 CTGTCATCAT TTGTACAAAC TCTTCGTAGT TTACCTGACC ATCCCCATCG ATGTCTGCTT
          181 CCCTGATCAT TTCATCAACC TCTTCATCTG TTAACTTCTC TCCAAGGTTT GTCATCACGT
          241 GGCGAAGCTC TGCTGCACTG ATGTAGCCAT TGCCATCCTT ATCAAACACA CGGAACGCTT
          301 CTCTAATTTC TTCTTCACTG TCTGTGTCTT TCATTTTTCT TGCCATCATT GTCAGGAACT
          361 CAGGGAAGTC GATTGTGCCA TTACCGTCGG CATCTACTTC ATTGATCATG TCCTGCAGCT
          421 CTGCTTCTGT GGGGTTCTGC CCCAGAGACC GCATCACCGT CCCCAGCTCC TTGGTTGTTA
          481 TTGTCCCATC CCCGTCCTTG TCAAATAGGG AGAAAGCCTC TTTAAATTCT GCGATCTGCT
          541 CTTCAGTCAG TTGGTCACGC GTGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT
          601 CCTTGAAGTC GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA
          661 CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGATGT
          721 AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC
          781 TGAAGCACTG CACGCCGTAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT
          841 TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCACCCT
          901 CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA
          961 CCACCCCGGT GAACAGCTCT CGCCCTTGCT CACATGCTCC CCTCGGGTAC GCCCTGTACA
         1021 GCTCGTCATG CCGAGAGTGA TCCTGCCGCC GTCACGAACT TCAAGCAAGA CATGTGATCG
        1081 CGCATCTCGT AGGATCATCG CAGTTGACCT GGGGTGTCTC AAGGTAATG
        //
        Primer: SV40pro_F_V2
        Sequence file: RDB14349_A6Iuc.seq check
        >06750_14349_A6Iu_SV40pro_F_V2_C01_07_ABI24.ab1
            1 TAGGCGTTTT GGAGCTAGGC TTTTGCAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC
           61 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
          121 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
          181 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
          241 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
          301 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
          361 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
          421 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
          481 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
          541 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
          601 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
          661 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG
          721 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT
          781 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC
          841 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA
          901 CCCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTTCGGA
          961 ATCGTTTTCC CGGGGACGCC CGGCTGGATG ATCTCAGCGC GGGGATCTCA TGCTGAGTCT
         1021 CGCCCACCCT ACGGGGAAGC CTACCTGAAA CACGGCAGCA GACATACTGA GGACCCGCGC
        1081 TATGAACGGC CAT
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Tallini, Y.N., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386.
        user report Fujii, Y., Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels. Sci. Rep. 7 (1): 13115 (2017). PMID 29030562.

        2022.02.09

        GNP_filter3_RDBDEP_html_220112.pl