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pN1-G-CaMP2 (#RDB06750)

Genetically-encoded fluorescent calcium sensor cDNA


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Tallini, Y.N., Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pN1-REST-mG1.6#X-1
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. Its fluorescence is brighter than that of G-CaMP1.6.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37C, LB+Kan
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence DQ381402 (DDBJ accession number)
Depositor|Developer Nakai, Junichi |

Distribution information

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Catalog # Resource name Shipping form Fee
RDB06750 pN1-G-CaMP2 DNA solution

How to cite this biological resource

Materials & Methods section:

The pN1-G-CaMP2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06750).

Reference section:

Tallini, Y.N., Ohkura, M., Choi, B.R., Ji, G., Imoto, K., Doran, R., Lee, J., Plan, P., Wilson, J., Xin, H.B., Sanbe, A., Gulick, J., Mathai, J., Robbins, J., Salama, G., Nakai, J., Kotlikoff, M.I., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tallini, Y.N., Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2. Proc. Natl. Acad. Sci. U S A., 103, 4753-4758 (2006). PMID 16537386. [PubMed] [Article] [RRC of NBRP]
user_report Fujii, Y., Astrocyte calcium waves propagate proximally by gap junction and distally by extracellular diffusion of ATP released from volume-regulated anion channels. Sci. Rep. 7 (1): 13115 (2017). PMID 29030562. [PubMed] [Article] [RRC of NBRP]