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pN1-G-CaMP1.6

Genetically-encoded fluorescent calcium sensor cDNA

Catalog number RDB06749
Resource name pN1-G-CaMP1.6
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. It showed brighter fluorescence than G-CaMP. The expressed protein has proper conformation at 28 degC but not at 37 degC.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 3,923 bp
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence DQ457005 (DDBJ accession number)
Depositor|Developer Nakai, Junichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Schedule A (GFP-A) [Link]
        Approval form by depositor [Word]
        Remarks ((Additional form)) Approval Form(FormD)
        Please use Schedule A (GFP-A) additionally.
        Please obtain written approval of the depositor.
        提供案内 (日本国内) [open/close]

        提供条件 寄託者に許可を取ること。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。研究成果の発表の時、謝辞に承諾者名を入れる。寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        Schedule A(GFP-A, 英語版のみ) [Link]
        寄託者による提供承諾書 [Word]
        備考 【追加提供依頼書】承諾書(FormD)
        Schedule A(GFP-A, 英語版のみ)が追加で必要です。
        寄託者の書面による提供承諾が必要です。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB06749 pN1-G-CaMP1.6 Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
        Ordering Information [in Japanese] [in English]

        References and tips

        Electronic file

        Sequence RDB06749z.seq
        Remarks, protocol and/or map (pdf) RDB06749.pdf

        Original reference

        original Ohkura, M., Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines. Anal. Chem., 77, 5861-5869 (2005). PMID 16159115.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content GFP Resources (English text)
        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Ohkura, M., Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines. Anal. Chem., 77, 5861-5869 (2005). PMID 16159115.

        2022.02.09

        GNP_filter3_RDBDEP_html_220112.pl