Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pN1-G-CaMP

Genetically-encoded fluorescent calcium sensor cDNA

Catalog number RDB06747
Resource name pN1-G-CaMP
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. The expressed protein has proper conformation at 28 degC but not at 37 degC.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AJ550759 (DDBJ accession number)
Depositor|Developer Nakai, Junichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        Schedule A (GFP-A) [Link]
        Approval form by depositor [Word]
        Remarks ((Additional form)) Approval Form(FormD)
        Please use Schedule A (GFP-A) additionally.
        Please obtain written approval of the depositor.
        提供案内 (日本国内) [open/close]

        提供条件 寄託者に許可を取ること。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。研究成果の発表の時、謝辞に承諾者名を入れる。寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        Schedule A(GFP-A, 英語版のみ) [Link]
        寄託者による提供承諾書 [Word]
        備考 【追加提供依頼書】承諾書(FormD)
        Schedule A(GFP-A, 英語版のみ)が追加で必要です。
        寄託者の書面による提供承諾が必要です。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB06747 pN1-G-CaMP DNA solution

        Ordering Information [in Japanese] [in English]
        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Sequence RDB06747z.seq
        Remarks, protocol and/or map (pdf) RDB06747.pdf

        Original reference

        original Nakai, J., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content GFP Resources (English text)
        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB06747_A3Egp1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV-Forward
        Sequence file: RDB06747_A3Ega.seq check
        >06747_06747_A3Eg_CMV-Forward_A09_03.ab1
            1 AGTTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
           61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGCCA
          121 CCATGGTCGA CTCATCACGT CGTAAGTGGA ATAAGACAGG TCACGCAGTC AGAGCTATAG
          181 GTCGGCTGAG CTCACTCGAG AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
          241 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
          301 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
          361 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
          421 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG GGCGGTACCG
          481 GAGGGAGCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
          541 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG
          601 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
          661 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
          721 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA
          781 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG
          841 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC
          901 TGGGGCACAA GCTGGAGTAC AACACGCGTG ACCAACTGAC TGAAGAGCAG ATCGCAGAAT
          961 TCAAAGAAGC TTTCTCCCTA TTTGACAAGG ACGGGGATGG GACAATAACA ACCAAAGGAG
         1021 CTGGGGGACG GTGATGCGGG TCTCTGGGGG CAGAACCCCC ACAGAAGCAG AGCTGCAGGA
        1081 CATGATCCAA TGAGTAGAAT GCGACCGTTA TGGCACAATC GAACTTTCCC CTTG
        //
        Primer: ME-1290Rv
        Sequence file: RDB06747_A3Egb.seq check
        >06747_06747_A3Eg_ME-1290Rv_B09_06.ab1
            1 AAGGTCAATT TAATTTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC
           61 AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC TAGAGTCGCG GCCGCTCACT
          121 TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG ACCATCCCCA TCGATGTCTG
          181 CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT CTCTCCAAGG TTTGTCATCA
          241 CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC CTTATCAAAC ACACGGAACG
          301 CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT CTTTCATTTT TCTTGCCATC ATTGTCAGGA
          361 ATTCAGGGAA GTCGATTGTG CCATTACCGT CGGCATCTAC TTCATTGATC ATGTCCTGCA
          421 GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCATCAC CGTCCCCAGC TCCTTGGTTG
          481 TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC TTCTTTGAAT TCTGCGATCT
          541 GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT
          601 CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT
          661 TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA
          721 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC
          781 GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA
          841 GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC
          901 CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG
          961 GCACCACCCC GGTGAACAGC TCCCTCGCCC TTTGCTCACC ATGCTCCCTC CGGGTACCGC
         1021 CCTTGTACAG CTCGTCCCAT GCCCGAGAGT GATCCCGGCG CGGGTCACGA ACTTCAGCAG
         1081 ACATGTTGAT CGCGGCTCTC GATGGGATCT TGGCTCAAGA CGAACTTGGG CTGCTTCAAG
        1141 GTTAAGTGTG TATG
        //
        Primer: SV40pro_F_V2
        Sequence file: RDB06747_A3Egc.seq check
        >06747_06747_A3Eg_SV40pro_F_V2_A01_01_ABI08.ab1
            1 TACAGCTTTT TGGAGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
           61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
          121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
          181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
          241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
          301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
          361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
          421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
          481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
          541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
          601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
          661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
          721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
          781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
          841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA
          901 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAAGGTT GGGCTTCGGA
          961 ATCGTTTTCC GGGACGCCGG CTGGGATGAT CCTCCAGCGC GGGGGATCTC ATGCTGGAGT
         1021 TCTTCGCCCA CCCCTACGGG GGAGCTAACT GAAAACACGA AGGAGAACAT TACTGATGAA
         1081 CCCGCGCTAA TGACGGCATT AAAAGACCGA TTAACGCCAC GGATGTTGGG GTCGTTTGAT
        1141 TCCA
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Nakai, J., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727.
        reference Wang, J.W., Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell, 112, 271-282 (2003). PMID 12553914.

        2022.02.09

        GNP_filter3_RDBDEP_html_220112.pl