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hPrP129V in pTA2

Plasmid clone of human prion cDNA

Catalog number RDB06717
Resource name hPrP129V in pTA2
Clone info. Plasmid clone of human prion cDNA. There is polymorphism at codon 129. This clone has Met to Val substitution at codon 129. Nucleotide sequence of 75 to 700 of AB300824.1 was cloned. Signal peptide and propeptide are not included.
Vector backbone pTA2 (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human hPrP 129V cDNA
Reference of nucleotide sequence AB300824 (DDBJ accession number)
Depositor|Developer Nakajima, Osamu | Kikuchi, Yutaka |
 
Other clones in our bank human PRNP (NCBI Gene 5621) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FEBS J., 275, 2965-2976 (2008))
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RDB06717 hPrP129V in pTA2 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06717_A3Jh.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB06717_A3Jha.seq check
>06717_06717_A3Jh_3_T7_20bp_F02_17.ab1
    1 TGCATGGTCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CCAATACTCG
   61 GAATTCCATA TGAGCAAGAA GCGCCCGAAG CCTGGAGGAT GGAACACTGG GGGCAGCCGA
  121 TACCCGGGGC AGGGCAGCCC TGGAGGCAAC CGCTACCCAC CTCAGGGCGG TGGTGGCTGG
  181 GGGCAGCCTC ATGGTGGTGG CTGGGGGCAG CCTCATGGTG GTGGCTGGGG GCAGCCCCAT
  241 GGTGGTGGCT GGGGACAGCC TCATGGTGGT GGCTGGGGTC AAGGAGGTGG CACCCACAGT
  301 CAGTGGAACA AGCCGAGTAA GCCAAAAACC AACATGAAGC ACATGGCTGG TGCTGCAGCA
  361 GCTGGGGCAG TGGTGGGGGG CCTTGGCGGC TACGTGCTGG GAAGTGCCAT GAGCAGGCCC
  421 ATCATACATT TCGGCAGTGA CTATGAGGAC CGTTACTATC GTGAAAACAT GCACCGTTAC
  481 CCCAACCAAG TGTACTACAG GCCCATGGAT GAGTACAGCA ACCAGAACAA CTTTGTGCAC
  541 GACTGCGTCA ATATCACAAT CAAGCAGCAC ACGGTCACCA CAACCACCAA GGGGGAGAAC
  601 TTCACCGAGA CCGACGTTAA GATGATGGAG CGCGTGGTTG AGCAGATGTG TATCACCCAG
661 TACGAGAGGG AATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040.

2021.03.25

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