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hPrP129V in pTA2

Plasmid clone of human prion cDNA

Catalog number RDB06717
Resource name hPrP129V in pTA2
Clone info. Plasmid clone of human prion cDNA. There is polymorphism at codon 129. This clone has Met to Val substitution at codon 129. Nucleotide sequence of 75 to 700 of AB300824.1 was cloned. Signal peptide and propeptide are not included.
Vector backbone pTA2 (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human hPrP 129V cDNA
Reference of insert sequence AB300824 (DDBJ accession number)
Depositor|Developer Nakajima, Osamu | Kikuchi, Yutaka |
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External Database
Human hPrP 129V
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FEBS J., 275, 2965-2976 (2008))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FEBS J., 275, 2965-2976 (2008))。
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          RDB06717 hPrP129V in pTA2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The hPrP129V in pTA2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06717).

          Reference section:

          Kikuchi, Y., Kakeya, T., Nakajima, O., Sakai, A., Ikeda, K., Yamaguchi, N., Yamazaki, T., Tanamoto, K., Matsuda, H., Sawada, J., Takatori, K., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB06717_A3Jh.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB06717_A3Jha.seq check
          >06717_06717_A3Jh_3_T7_20bp_F02_17.ab1
              1 TGCATGGTCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CCAATACTCG
             61 GAATTCCATA TGAGCAAGAA GCGCCCGAAG CCTGGAGGAT GGAACACTGG GGGCAGCCGA
            121 TACCCGGGGC AGGGCAGCCC TGGAGGCAAC CGCTACCCAC CTCAGGGCGG TGGTGGCTGG
            181 GGGCAGCCTC ATGGTGGTGG CTGGGGGCAG CCTCATGGTG GTGGCTGGGG GCAGCCCCAT
            241 GGTGGTGGCT GGGGACAGCC TCATGGTGGT GGCTGGGGTC AAGGAGGTGG CACCCACAGT
            301 CAGTGGAACA AGCCGAGTAA GCCAAAAACC AACATGAAGC ACATGGCTGG TGCTGCAGCA
            361 GCTGGGGCAG TGGTGGGGGG CCTTGGCGGC TACGTGCTGG GAAGTGCCAT GAGCAGGCCC
            421 ATCATACATT TCGGCAGTGA CTATGAGGAC CGTTACTATC GTGAAAACAT GCACCGTTAC
            481 CCCAACCAAG TGTACTACAG GCCCATGGAT GAGTACAGCA ACCAGAACAA CTTTGTGCAC
            541 GACTGCGTCA ATATCACAAT CAAGCAGCAC ACGGTCACCA CAACCACCAA GGGGGAGAAC
            601 TTCACCGAGA CCGACGTTAA GATGATGGAG CGCGTGGTTG AGCAGATGTG TATCACCCAG
          661 TACGAGAGGG AATC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kikuchi, Y., Hypoxia induces expression of a GPI-anchorless splice variant of the prion protein. FEBS J., 275, 2965-2976 (2008). PMID 18445040. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl