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pCMFlag_hsc-Myc

Expression vector of human c-Myc.

Catalog number RDB06671
Resource name pCMFlag_hsc-Myc
Alternative name SET-0220_2
Clone info. Expression vector of human c-Myc. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: ctggatttttttcgggtagtgga; reverse primer: ttacgcacaagagttccgtagct.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human c-Myc cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human c-Myc
          Reference sequence
            

          Distribution information

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          RDB06671 pCMFlag_hsc-Myc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06671.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12838_A4H6.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12838_A4H6a.seq check
          >06671_12838_A4H6_CMV-Foward_A07_01.ab1
              1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGCGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
            181 CGGCCCTGGA TTTTTTTCGG GTAGTGGAAA ACCAGCAGCC TCCCGCGACG ATGCCCCTCA
            241 ACGTTAGCTT CACCAACAGG AACTATGACC TCGACTACGA CTCGGTGCAG CCGTATTTCT
            301 ACTGCGACGA GGAGGAGAAC TTCTACCAGC AGCAGCAGCA GAGCGAGCTG CAGCCCCCGG
            361 CGCCCAGCGA GGATATCTGG AAGAAATTCG AGCTGCTGCC CACCCCGCCC CTGTCCCCTA
            421 GCCGCCGCTC CGGGCTCTGC TCGCCCTCCT ACGTTGCGGT CACACCCTTC TCCCTTCGGG
            481 GAGACAACGA CGGCGGTGGC GGGAGCTTCT CCACGGCCGA CCAGCTGGAG ATGGTGACCG
            541 AGCTGCTGGG AGGAGACATG GTGAACCAGA GTTTCATCTG CGACCCGGAC GACGAGACCT
            601 TCATCAAAAA CATCATCATC CAGGACTGTA TGTGGAGCGG CTTCTCGGCC GCCGCCAAGC
            661 TCGTCTCAGA GAAGCTGGGC CTCCTACCAG GCTGCGCGCA AAGACAGCGG CAGCCCGAAC
            721 CCGCCGCGGC ACAGCGTCTG CTCACTCAGC TTGTACTGCA GATCTGACGC CGCCGCCTCA
            781 GAGTGCATCG ACCCTCGTGG TCTTCCCTAC CTCTCACGAC AGCAGCTCGC CAGTCTGGCG
            841 CCTCCGCAGA CTCAGCGCTT CTCTCGTCCT CGGAATTCTC TGCTCTCTCG ACGGATCCTC
            901 CCCGCAAGCA GTCTCGAGCC TGGTGTTCAT GAGGAGAACC GCTCACACAA GATCGAACTT
            961 GAGAGAACTG AAATGAGGGA ATCAGTGTTC TGGAAAAGCA GTCTGCAAGT CTACTGGAAT
          1021 CTC
          //
          Primer: BGH_rev
          Sequence file: RDB12838_A4H6b.seq check
          >06671_12838_A4H6_BGHrev_B07_04.ab1
              1 GACTTTACTT TTAGGTGAAA CTATAGAAAA GGGGCCCTCT AGATGCATGC TCGAGCGGCC
             61 TTACGCACAA GAGTTCCGTA CCTGTTCAAG TTTGTGTTTC AACTGTTCTC GTCGTTTCCG
            121 CAACAAGTCC TCTTCAGAAA TGAGCTTTTG CTCCTCTGCT TGGACGGACA GGATGTATGC
            181 TGTGGCTTTT TTAAGGATAA CTACCTTGGG GGCCTTTTCA TTGTTTTCCA ACTCCGGGAT
            241 CTGGTCACGC AGGGCAAAAA AGCTCCGTTT TAGCTCGTTC CTCCTCTGGC GCTCCAAGAC
            301 GTTGTGTGTT CGCCTCTTGA CATTCTCCTC GGTGTCCGAG GACCTGGGGC TGGTGCATTT
            361 TCGGTTGTTG CTGATCTGTC TCAGGACTCT GACACTGTCC AACTTGACCC TCTTGGCAGC
            421 AGGATAGTCC TTCCGAGTGG AGGGAGGCGC TGCGTAGTTG TGCTGATGTG TGGAGACGTG
            481 GCACCTCTTG AGGACCAGTG GGCTGTGAGG AGGTTTGCTG TGGCCTCCAT CAGAAGTGAT
            541 CCAACTCTGA CCTTTTGCCA GGAGCCTGCC TCTTTTCCAC AGAAACAACA TCGATTTCTT
            601 CCTCATCTTC TTGTTCCTCC TCAGAGTCGC TGCTGGTGGT GGGCGGTGTC TCCTCATGGA
          661 GCACCCAGGG GCTCGGGGCT GCCCTGCGGG GAGGAACTCC CTCGAGGGAT AGCAAAGAA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Seira, N., Cellular density-dependent increases in HIF-1alpha compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region. Pharmacol. Res. Perspect. 6 (6): e00441 (2018). PMID 30455960.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl