Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsc-Myc (#RDB06671)

Expression vector of human c-Myc.

Alternative name SET-0220_2
Clone info. Expression vector of human c-Myc. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: ctggatttttttcgggtagtgga; reverse primer: ttacgcacaagagttccgtagct.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human c-Myc cDNA
Depositor|Developer DNA Bank, |
 
Sequence (full) RDB06671hts01.seq checkAssembled from experimentally sequenced data.
Remarks, protocol and/or map (pdf) RDB06671.pdf
Publication Unpublished bioresource
Test sheet RDB12838_A4H6p1-4.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06671 pCMFlag_hsc-Myc DNA solution

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsc-Myc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06671).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Seira, N., Cellular density-dependent increases in HIF-1alpha compete with c-Myc to down-regulate human EP4 receptor promoter activity through Sp-1-binding region. Pharmacol. Res. Perspect. 6 (6): e00441 (2018). PMID 30455960. [link to RRC of NBRP]