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pCMFlag_hsBIRC5 (#RDB06597)

Expression vector of human BIRC5/API4.

Alternative name SET-0187_2
Clone info. Expression vector of human BIRC5/API4. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano KAT00573. PCR cloning, forward primer: ggtgccccgacgttgccccctg; reverse primer: tcaatccatggcagccagctgctcg.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human BIRC5/API4 cDNA
Depositor|Developer DNA Bank, |
 

Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。

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RDB06597 pCMFlag_hsBIRC5 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsBIRC5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06597).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12969_A4J1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12969_A4J1a.seq check
>06597_12969_A4J1_CMV-Foward_A03_03.ab1
    1 TTGCTTCAAC TCCGGCCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GAATTCACCA TGGACTACAA GGACGATGAC
  181 GATAAGGCGG CCGGTGCCCC GACGTTGCCC CCTGCCTGGC AGCCCTTTCT CAAGGACCAC
  241 CGCATCTCTA CATTCAAGAA CTGGCCCTTC TTGGAGGGCT GCGCCTGCAC CCCGGAGCGG
  301 ATGGCCGAGG CTGGCTTCAT CCACTGCCCC ACTGAGAACG AGCCAGACTT GGCCCAGTGT
  361 CTCTTCTGCT TCAAGGAGCT GGAAGGCTGG GAGCCAGATG ACGACCCCAT AGAGGAACAT
  421 AAAAAGCATT CGTCCGGTTG CGCTTTCCTT TCTGTCAAGA AGCAGTTTGA AGAATTAACC
  481 CTTGGTGAAT TTTTGAAACT GGACAGAGAA AGAGCCAAGA ACAAAATTGC AAAGGAAACC
  541 AACAATAAGA AGAAAGAATT TGAGGAAACT GCGAAGAAAG TGCGCCGTGC CATCGAGCAG
  601 CTGGCTGCCA TGGATTGAGG CCGCTCGAGC ATGCATCTAG AGGGCCCTAT TCTATAGTGT
  661 CACCTAAATG CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT
  721 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT
  781 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG
  841 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG
  901 GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC GAGGGGGGAT
  961 CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCCGGGT GTGGTGGTTA CGCGCAGCGT
 1021 GAACCGCTAC ACTTGCCAGC GCCCTAGCGC CGCTCTTTCG CTTTTCTCCC GTCGTTTCTC
 1081 GGCCACGGTT CGGCGCTTAC CCGGTTCAGG CTCTAATCCG GGGGCTCCCT TTGAGGGGAT
1141 TTCCCGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles