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pGFH81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06591
Resource name pGFH81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06591 pGFH81 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06591z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14477_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14477_A6L7a.seq check
            >06591_14477_A6L7_nmt1Fw_G11_20_ABI24.ab1
                1 ATGAATTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG
              181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC
              241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCGGATA
              301 TCATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG
              361 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG
              421 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC
              481 TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC
              541 AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA ACTATATTTT
              601 TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG
              661 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA
              721 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG
              781 GAATCAAAGT TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG
              841 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT
              901 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGGTC
              961 CTTCTTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGG CATGGATGAA CTATACAACC
             1021 CATGGGACTA CAGGACGACG ATGACAACAT CACACATCAC ACTGAAGATC TCGAGCCGGC
            1081 CGATCGCTAA CTAACTTAAA GGGGCAAAAA AATGA
            //
            Primer: nmt1ter_R
            Sequence file: RDB14477_A6L7b.seq check
            >06591_14477_A6L7_nmt1ter_R_H11_23_ABI24.ab1
                1 GATTTCTAAG TAAACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT
               61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG
              121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA
              181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT
              241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA
              301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA GTGGTGATGG
              361 TGGTGATGTT TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA
              421 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG
              481 GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG
              541 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA
              601 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG
              661 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT
              721 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAAACAAGG GTATCACCTT CAAACTTGAC
              781 TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA
              841 GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA
              901 AAAGCATTGA ACACCCATAA CAGAAAGTAG TGACAAGTGT TGGGCCACGG AAACAAGGTA
              961 GGTTTTCCAG TAGTTGCAAA TAATTTTAAT GGGTAAAGGT TTTCCCGTTA TGTTTGCATC
             1021 ACCCTTTCAC CCTTCTCCAC TGGAACAGAA AAATTTGTGT GCCCAGTACC ATCACCAATC
             1081 GAATTTCCAA CGAGGATTGG GGACCACTCC AGGTGGAAGA AGTTCCTTCT TCCTTTTTAC
            1141 CTGCTCAACA TG
            //
            Primer: f1ori_R3
            Sequence file: RDB14477_A6L7c.seq check
            >06591_14477_A6L7_f1ori_R3_B12_06_ABI24.ab1
                1 GCAATTGGGG GCTTGGAGTA TGCGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC
               61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
              121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
              181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT
              241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT
              301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG
              361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT
              421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG
              481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT
              541 AGGGGCGAGC CGAGACAAAA AGGGGATACA AGGGCATCGT CTATAATTAT AGCTAAAAAA
              601 AAGTATTTTA ATTTGTATTT TTTGTAATTT TATTTCTCAT TGTTTTACTT AAAATGAATG
              661 CGAATTAGAG AAAAACTTTC TTCGAAATGT CAAAATAAGC TCAGCAAAAT ATACAATTTT
              721 ATGGGAAAGC GATCAGCAAC TTATTCTCCG TTGTAGATTC TTTTTCTTCA TAAATTCCAA
              781 CAATGTAACT GCCAATTGAT TTTTCTCTAG ATGCTAATAA ATTTGAAACT AATGAATATA
              841 ATAACACAGG TTTTGATAAA TTGTAAACTC TTACTCAAAA AAGAATGTTA AACTAAATTA
              901 ACTTTTTATA AGCCCATGGC GTTAAAGAGG ATAGTTAAAT CTACAATTTT TTAATTTATG
              961 TCACATTGTC TTGCAAATAA TTCTATAATT TCGCTAACTG CTGTTAGAAC ATATACCTCA
             1021 ATTGGCTAAA TGGCCACTAA TTCCAGAGAA CTGCAATTGC ACTTCTGACT GAGCGCTTAA
            1081 TCA
            //
            Primer: LEU2pro_F
            Sequence file: RDB14477_A6L7d.seq check
            >06591_14477_A6L7_LEU2pro_F_A12_03_ABI24.ab1
                1 AAACTTGCAG CTGACATATT ATTTAAGGAC CTATTGTTTT TTCCAATAGG TGGTTAGCAA
               61 TCGTCTTACT TTCTAACTTT TCTTACCTTT TACATTTCAG CAATATATAT ATATATTTCA
              121 AGGATATACC ATTCTAATGT CTGCCCCTAA GAAGATCGTC GTTTTGCCAG GTGACCACGT
              181 TGGTCAAGAA ATCACAGCCG AAGCCATTAA GGTTCTTAAA GCTATTTCTG ATGTTCGTTC
              241 CAATGTCAAG TTCGATTTCG AAAATCATTT AATTGGTGGT GCTGCTATCG ATGCTACAGG
              301 TGTTCCACTT CCAGATGAGG CGCTGGAAGC CTCCAAGAAG GCTGATGCCG TTTTGTTAGG
              361 TGCTGTGGGT GGTCCTAAAT GGGGTACCGG TAGTGTTAGA CCTGAACAAG GTTTACTAAA
              421 AATCCGTAAA GAACTTCAAT TGTACGCCAA CTTAAGACCA TGTAACTTTG CATCCGACTC
              481 TCTTTTAGAC TTATCTCCAA TCAAGCCACA ATTTGCTAAA GGTACTGACT TCGTTGTTGT
              541 CAGAGAATTA GTGGGAGGTA TTTACTTTGG TAAGAGAAAG GAAGACGATG GTGATGGTGT
              601 CGCTTGGGAT AGTGAACAAT ACACCGTTCC AGAAGTGCAA AGAATCACAA GAATGGCCGC
              661 TTTCATGGCC CTACAACATG AGCCACCATT GCCTATTTGG TCCTTGGATA AAGCTAATGT
              721 TTTGGCCTCT TCAAGATTAT GGAGAAAAAC TGTGGAGGAA ACCATCAAGA ACGAATTCCC
              781 TACATTGAAG GTTCAACATC AATTGATTGA TTCTGCCGCC ATGATCCTAG TTAAGAACCC
              841 AACCCACCTA AATGGTATTA TAATCACCAG CAACATGTTT GGTGACATCA TCTCCGATGA
              901 AGCCTCCCGT TATCCCAGGT TCCCTTGGGG TTTGTTGCCA TCTGCGTCCT TGGGCCCTCT
              961 TTGCCCAGAC AAGAACACCG CATTTTGGGT TTGGTACGGA TCAATGCCCA CGGGTTCTGC
             1021 TCCCAGAATT TTGCCCAAAG AATAAGTCAA ACCCTATCGC CCACTATTCT TGTCTGCTTG
            1081 CAAATGATGT GAATGGTCAT GGACCTTGGC CCTGAGAGGT TAGAGGCCAT TTGAGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl