Resource data sheet
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pGFH81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06591
Resource name pGFH81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06591 pGFH81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06591z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14477_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14477_A6L7a.seq
>06591_14477_A6L7_nmt1Fw_G11_20_ABI24.ab1
    1 ATGAATTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG
  181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC
  241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCGGATA
  301 TCATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT GAATTAGATG
  361 GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT GCAACATACG
  421 GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG TGGCCAACAC
  481 TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT CACATGAAAC
  541 AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA ACTATATTTT
  601 TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT GATACCCTTG
  661 TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT CTTGGACACA
  721 AATTGGAATA CAACTATAAC TCACACAATG TATACATCAT GGCAGACAAA CAAAAGAATG
  781 GAATCAAAGT TAACTTCAAA ATTAGACACA ACATTGAAGA TGGAAGCGTT CAACTAGCAG
  841 ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA GACAACCATT
  901 ACCTGTCCAC ACAATCTGCC CTTTCGAAAG ATCCCAACGA AAAGAGAGAC CACATGGGTC
  961 CTTCTTTGAG TTTGTAACAG CTGCTGGGAT TACACATGGG CATGGATGAA CTATACAACC
 1021 CATGGGACTA CAGGACGACG ATGACAACAT CACACATCAC ACTGAAGATC TCGAGCCGGC
1081 CGATCGCTAA CTAACTTAAA GGGGCAAAAA AATGA
//
Primer: nmt1ter_R RDB14477_A6L7b.seq
>06591_14477_A6L7_nmt1ter_R_H11_23_ABI24.ab1
    1 GATTTCTAAG TAAACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT
   61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG
  121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA
  181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT
  241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA
  301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA GTGGTGATGG
  361 TGGTGATGTT TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA
  421 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG
  481 GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG
  541 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA
  601 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG
  661 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT
  721 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAAACAAGG GTATCACCTT CAAACTTGAC
  781 TTCAGCACGT GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA
  841 GCCTTCGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA
  901 AAAGCATTGA ACACCCATAA CAGAAAGTAG TGACAAGTGT TGGGCCACGG AAACAAGGTA
  961 GGTTTTCCAG TAGTTGCAAA TAATTTTAAT GGGTAAAGGT TTTCCCGTTA TGTTTGCATC
 1021 ACCCTTTCAC CCTTCTCCAC TGGAACAGAA AAATTTGTGT GCCCAGTACC ATCACCAATC
 1081 GAATTTCCAA CGAGGATTGG GGACCACTCC AGGTGGAAGA AGTTCCTTCT TCCTTTTTAC
1141 CTGCTCAACA TG
//
Primer: f1ori_R3 RDB14477_A6L7c.seq
>06591_14477_A6L7_f1ori_R3_B12_06_ABI24.ab1
    1 GCAATTGGGG GCTTGGAGTA TGCGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC
   61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
  121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
  181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT
  241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT
  301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG
  361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT
  421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG
  481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT
  541 AGGGGCGAGC CGAGACAAAA AGGGGATACA AGGGCATCGT CTATAATTAT AGCTAAAAAA
  601 AAGTATTTTA ATTTGTATTT TTTGTAATTT TATTTCTCAT TGTTTTACTT AAAATGAATG
  661 CGAATTAGAG AAAAACTTTC TTCGAAATGT CAAAATAAGC TCAGCAAAAT ATACAATTTT
  721 ATGGGAAAGC GATCAGCAAC TTATTCTCCG TTGTAGATTC TTTTTCTTCA TAAATTCCAA
  781 CAATGTAACT GCCAATTGAT TTTTCTCTAG ATGCTAATAA ATTTGAAACT AATGAATATA
  841 ATAACACAGG TTTTGATAAA TTGTAAACTC TTACTCAAAA AAGAATGTTA AACTAAATTA
  901 ACTTTTTATA AGCCCATGGC GTTAAAGAGG ATAGTTAAAT CTACAATTTT TTAATTTATG
  961 TCACATTGTC TTGCAAATAA TTCTATAATT TCGCTAACTG CTGTTAGAAC ATATACCTCA
 1021 ATTGGCTAAA TGGCCACTAA TTCCAGAGAA CTGCAATTGC ACTTCTGACT GAGCGCTTAA
1081 TCA
//
Primer: LEU2pro_F RDB14477_A6L7d.seq
>06591_14477_A6L7_LEU2pro_F_A12_03_ABI24.ab1
    1 AAACTTGCAG CTGACATATT ATTTAAGGAC CTATTGTTTT TTCCAATAGG TGGTTAGCAA
   61 TCGTCTTACT TTCTAACTTT TCTTACCTTT TACATTTCAG CAATATATAT ATATATTTCA
  121 AGGATATACC ATTCTAATGT CTGCCCCTAA GAAGATCGTC GTTTTGCCAG GTGACCACGT
  181 TGGTCAAGAA ATCACAGCCG AAGCCATTAA GGTTCTTAAA GCTATTTCTG ATGTTCGTTC
  241 CAATGTCAAG TTCGATTTCG AAAATCATTT AATTGGTGGT GCTGCTATCG ATGCTACAGG
  301 TGTTCCACTT CCAGATGAGG CGCTGGAAGC CTCCAAGAAG GCTGATGCCG TTTTGTTAGG
  361 TGCTGTGGGT GGTCCTAAAT GGGGTACCGG TAGTGTTAGA CCTGAACAAG GTTTACTAAA
  421 AATCCGTAAA GAACTTCAAT TGTACGCCAA CTTAAGACCA TGTAACTTTG CATCCGACTC
  481 TCTTTTAGAC TTATCTCCAA TCAAGCCACA ATTTGCTAAA GGTACTGACT TCGTTGTTGT
  541 CAGAGAATTA GTGGGAGGTA TTTACTTTGG TAAGAGAAAG GAAGACGATG GTGATGGTGT
  601 CGCTTGGGAT AGTGAACAAT ACACCGTTCC AGAAGTGCAA AGAATCACAA GAATGGCCGC
  661 TTTCATGGCC CTACAACATG AGCCACCATT GCCTATTTGG TCCTTGGATA AAGCTAATGT
  721 TTTGGCCTCT TCAAGATTAT GGAGAAAAAC TGTGGAGGAA ACCATCAAGA ACGAATTCCC
  781 TACATTGAAG GTTCAACATC AATTGATTGA TTCTGCCGCC ATGATCCTAG TTAAGAACCC
  841 AACCCACCTA AATGGTATTA TAATCACCAG CAACATGTTT GGTGACATCA TCTCCGATGA
  901 AGCCTCCCGT TATCCCAGGT TCCCTTGGGG TTTGTTGCCA TCTGCGTCCT TGGGCCCTCT
  961 TTGCCCAGAC AAGAACACCG CATTTTGGGT TTGGTACGGA TCAATGCCCA CGGGTTCTGC
 1021 TCCCAGAATT TTGCCCAAAG AATAAGTCAA ACCCTATCGC CCACTATTCT TGTCTGCTTG
1081 CAAATGATGT GAATGGTCAT GGACCTTGGC CCTGAGAGGT TAGAGGCCAT TTGAGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl