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pGFH41

S. pombe expression vector with nmt1 promoter

Catalog number RDB06590
Resource name pGFH41
Clone info. S. pombe 3' ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter.
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06590 pGFH41 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06590z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14476_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14476_A6L7a.seq check
            >06590_14476_A6L7_nmt1Fw_C11_08_ABI24.ab1
                1 AGGAAATTTT TTTTAAAATC AGGGAAAACG TAAACTCTCG GCTAACTGGA TGGTTCAAGT
               61 CACCCAACGA TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT
              121 GTCATTCGGC AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT
              181 TGAAGATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT
              241 TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC
              301 TGGATCCGGA TATCATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG
              361 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG
              421 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC
              481 CGTGGCCAAC ACTTGTCACT ACTTTCTGTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG
              541 ATCACATGAA ACAGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGCTAT GTACAGGAAA
              601 GAACTATATT TTTCAAAGAT GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG
              661 GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA
              721 TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA
              781 AACAAAAGAA TGGAATCAAA GTTAACTTCA AAATTAGACA CAACATTGAA GATGGGAAGC
              841 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCCAATT GGCGATGGCC CTGTCCTTTT
              901 ACCAGACAAC CATTTACCTG TCCACACAAT CTGGCCCTTT TCGAAAGATC CCAACGAAAA
              961 GAGAGACCCA CATGGGTCCC TTCTTTGAAG TTTGGTAACA GCTGCTGGGA ATTACACATG
             1021 GGCATGGGAT GAAACTATAC AACCCCATGG ACTACAAGGA CGAACGATGA ACAAACATCA
             1081 CTACAATCAC CACTGAGATC TCGAAGTCCG GGCGGCCGCC TAAGCTTAAA CCTTAAGGGC
            1141 TCATCAACAA ACGGCA
            //
            Primer: nmt1ter_R
            Sequence file: RDB14476_A6L7b.seq check
            >06590_14476_A6L7_nmt1ter_R_D11_11_ABI24.ab1
                1 GTTGTGTAGT TACTCATGTA TAAACGTTTT GGGTTCATAT TTTTGGGCCA CGTGGTTTAT
               61 AAAAATTCTT AACTACACCA CTCGGTATCC CGCACCCGTC TACGTTTCTA CGATTTCGAG
              121 CTAATATTGA GTAAAGTGAT TAGCAAAAAA AATAAAAGTA CTCGTTGTCG GAGATCAAGA
              181 ATTTTTCTAT TATCTCATCT AAACCACTTT CTAAAAGCGA AAAACAAAAT CGTAATATGC
              241 AGCTTGAATG GGCTTCCATA GTTTGAAAGA AAAACCCTAG CAGTACTGGC AAGGGAGACA
              301 TTCCTTTTAC CCTTAGTTAG TTAGCGGCCG CCCGGGCTCG AGATCTCAGT GGTGATGGTG
              361 GTGATGTTTG TCATCGTCGT CCTTGTAGTC CATGGGTTTG TATAGTTCAT CCATGCCATG
              421 TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG TGGTCTCTCT TTTCGTTGGG
              481 ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG TCTGGTAAAA GGACAGGGCC
              541 ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT TGAACGCTTC CATCTTCAAT
              601 GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT TGTTTGTCTG CCATGATGTA
              661 TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA AGAATGTTTC CATCTTCTTT
              721 AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA TCACCTTCAA ACTTGACTTC
              781 AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA GTTCTTTCCT GTACATAGCC
              841 TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG TGATCTGGGT ATCTTGAAAA
              901 GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC CACGGAAACA GTAGTTTTTC
              961 CAGTAGTGCA AATAAATTTA AACGGTAAGT TTTCCCGTAT GTTGCATCAC CCTTTCACCC
             1021 CTCTCCACTG ACAGAAAATT TGTGCCCATT TAACATCACC ATCTACTCAA CAGAATTGGA
            1081 CCACTTCCAG TGAAAAAGGT CGTCTCCTTT TAACCTCATG CGAT
            //
            Primer: f1ori_R3
            Sequence file: RDB14476_A6L7c.seq check
            >06590_14476_A6L7_f1ori_R3_F11_17_ABI24.ab1
                1 GGAATGGGGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
              241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
              301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
              361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
              421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
              481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
              541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
              601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
              661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
              721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
              781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
              841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT
              901 TTATAAAGGC TCATGGGCGT TAAAGAGGAT AGTAAAAAAT TCTACAATTT TTTTAAATTT
              961 AATGTCACAA ATTGTCTTTG CGAAATATAA TTTCTAATTA AATTTTCGCT AACTGCTGTT
             1021 TTAGTACTAT TACTTTCAAT ATGCCCTTTA TATGCAACTT TAATTCATTG AGGAATGCAA
            1081 TATTTTGCAT TCTTCTTGTA CTGAGCGCTT TTTTATATTC CAACTTTAG
            //
            Primer: LEU2pro_F
            Sequence file: RDB14476_A6L7d.seq check
            >06590_14476_A6L7_LEU2pro_F_E11_14_ABI24.ab1
                1 AATCCTTGGA GGTTGACAAT ATTATTTAAG GACCTATTGT TTTTTCCAAT AGGTGGTTAG
               61 CAATCGTCTT ACTTTCTAAC TTTTCTTACC TTTTACATTT CAGCAATATA TATATATATT
              121 TCAAGGATAT ACCATTCTAA TGTCTGCCCC TAAGAAGATC GTCGTTTTGC CAGGTGACCA
              181 CGTTGGTCAA GAAATCACAG CCGAAGCCAT TAAGGTTCTT AAAGCTATTT CTGATGTTCG
              241 TTCCAATGTC AAGTTCGATT TCGAAAATCA TTTAATTGGT GGTGCTGCTA TCGATGCTAC
              301 AGGTGTTCCA CTTCCAGATG AGGCGCTGGA AGCCTCCAAG AAGGCTGATG CCGTTTTGTT
              361 AGGTGCTGTG GGTGGTCCTA AATGGGGTAC CGGTAGTGTT AGACCTGAAC AAGGTTTACT
              421 AAAAATCCGT AAAGAACTTC AATTGTACGC CAACTTAAGA CCATGTAACT TTGCATCCGA
              481 CTCTCTTTTA GACTTATCTC CAATCAAGCC ACAATTTGCT AAAGGTACTG ACTTCGTTGT
              541 TGTCAGAGAA TTAGTGGGAG GTATTTACTT TGGTAAGAGA AAGGAAGACG ATGGTGATGG
              601 TGTCGCTTGG GATAGTGAAC AATACACCGT TCCAGAAGTG CAAAGAATCA CAAGAATGGC
              661 CGCTTTCATG GCCCTACAAC ATGAGCCACC ATTGCCTATT TGGTCCTTGG GATAAAGCTA
              721 ATGTTTTGGC CCTCTTCAAG ATTATGGAGA AAAACTGTGG AGGGAAACCC ATCAAGAACG
              781 AATTCCCCTA CATTGAAAGG TTCAAACATC AATTGATTGA TTCTGCCGCC ATGATCCTAG
              841 TTTAAGAACC CAACCCACCT AAATGGGTAT TATAATCACC AGCAACATTG TTTGGTGACA
              901 TCATCTCCCG ATGAAAGCCT CCCGTTATCC CCAGGTTCCT TTGGGTTTGT TGGCCAATCT
              961 GCCGTCCTTT GGCCTCTTTG CCCAGACAAA GAAACACCCG CAATTATGTT TGTTACGAAT
             1021 CCATGCCACC GGTTCTGGCT TCCAGATTTG GCCAAAAGAA GTAGCGTCCA ACGCCTACTC
             1081 TGGCGCACGT CGATCCTTGG TTCTGCCTGG CCATTGAATG TGCGAAATTG TTCCATTTGA
            1141 ACCTTGGTCT GGAAGAAAAG GTATAAGGGC CCCATGTCGA GA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl