Sequencing and PCR primers for primer information.
S. pombe expression vector with nmt1 promoter
| Catalog number | RDB06590 |
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| Resource name | pGFH41 |
| Clone info. | S. pombe 3' ter end GFP(S65C)-FLAG-His6-tagged vector with nmt1 (medium strength) promoter. |
| Vector backbone | pREP41 (Plasmid) |
| Selectable markers | Amp^r |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06590 | pGFH41 | DNA solution |
Electronic file
| Electronic file | Sequence RDB06590z.seq from Depositor |
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Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14476_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | RDB14476_A6L7a.seq |
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>06590_14476_A6L7_nmt1Fw_C11_08_ABI24.ab1 1 AGGAAATTTT TTTTAAAATC AGGGAAAACG TAAACTCTCG GCTAACTGGA TGGTTCAAGT 61 CACCCAACGA TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT 121 GTCATTCGGC AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT 181 TGAAGATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT 241 TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC 301 TGGATCCGGA TATCATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG 361 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG 421 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC 481 CGTGGCCAAC ACTTGTCACT ACTTTCTGTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG 541 ATCACATGAA ACAGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGCTAT GTACAGGAAA 601 GAACTATATT TTTCAAAGAT GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG 661 GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA 721 TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA 781 AACAAAAGAA TGGAATCAAA GTTAACTTCA AAATTAGACA CAACATTGAA GATGGGAAGC 841 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCCAATT GGCGATGGCC CTGTCCTTTT 901 ACCAGACAAC CATTTACCTG TCCACACAAT CTGGCCCTTT TCGAAAGATC CCAACGAAAA 961 GAGAGACCCA CATGGGTCCC TTCTTTGAAG TTTGGTAACA GCTGCTGGGA ATTACACATG 1021 GGCATGGGAT GAAACTATAC AACCCCATGG ACTACAAGGA CGAACGATGA ACAAACATCA 1081 CTACAATCAC CACTGAGATC TCGAAGTCCG GGCGGCCGCC TAAGCTTAAA CCTTAAGGGC 1141 TCATCAACAA ACGGCA // |
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| Primer: nmt1ter_R | RDB14476_A6L7b.seq |
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>06590_14476_A6L7_nmt1ter_R_D11_11_ABI24.ab1 1 GTTGTGTAGT TACTCATGTA TAAACGTTTT GGGTTCATAT TTTTGGGCCA CGTGGTTTAT 61 AAAAATTCTT AACTACACCA CTCGGTATCC CGCACCCGTC TACGTTTCTA CGATTTCGAG 121 CTAATATTGA GTAAAGTGAT TAGCAAAAAA AATAAAAGTA CTCGTTGTCG GAGATCAAGA 181 ATTTTTCTAT TATCTCATCT AAACCACTTT CTAAAAGCGA AAAACAAAAT CGTAATATGC 241 AGCTTGAATG GGCTTCCATA GTTTGAAAGA AAAACCCTAG CAGTACTGGC AAGGGAGACA 301 TTCCTTTTAC CCTTAGTTAG TTAGCGGCCG CCCGGGCTCG AGATCTCAGT GGTGATGGTG 361 GTGATGTTTG TCATCGTCGT CCTTGTAGTC CATGGGTTTG TATAGTTCAT CCATGCCATG 421 TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG TGGTCTCTCT TTTCGTTGGG 481 ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG TCTGGTAAAA GGACAGGGCC 541 ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT TGAACGCTTC CATCTTCAAT 601 GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT TGTTTGTCTG CCATGATGTA 661 TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA AGAATGTTTC CATCTTCTTT 721 AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA TCACCTTCAA ACTTGACTTC 781 AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA GTTCTTTCCT GTACATAGCC 841 TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG TGATCTGGGT ATCTTGAAAA 901 GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC CACGGAAACA GTAGTTTTTC 961 CAGTAGTGCA AATAAATTTA AACGGTAAGT TTTCCCGTAT GTTGCATCAC CCTTTCACCC 1021 CTCTCCACTG ACAGAAAATT TGTGCCCATT TAACATCACC ATCTACTCAA CAGAATTGGA 1081 CCACTTCCAG TGAAAAAGGT CGTCTCCTTT TAACCTCATG CGAT // |
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| Primer: f1ori_R3 | RDB14476_A6L7c.seq |
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>06590_14476_A6L7_f1ori_R3_F11_17_ABI24.ab1 1 GGAATGGGGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC 61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC 121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA 181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC 241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG 301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC 361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT 421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA 481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG 541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT 601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT 661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG 721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC 781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG 841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT 901 TTATAAAGGC TCATGGGCGT TAAAGAGGAT AGTAAAAAAT TCTACAATTT TTTTAAATTT 961 AATGTCACAA ATTGTCTTTG CGAAATATAA TTTCTAATTA AATTTTCGCT AACTGCTGTT 1021 TTAGTACTAT TACTTTCAAT ATGCCCTTTA TATGCAACTT TAATTCATTG AGGAATGCAA 1081 TATTTTGCAT TCTTCTTGTA CTGAGCGCTT TTTTATATTC CAACTTTAG // |
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| Primer: LEU2pro_F | RDB14476_A6L7d.seq |
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>06590_14476_A6L7_LEU2pro_F_E11_14_ABI24.ab1 1 AATCCTTGGA GGTTGACAAT ATTATTTAAG GACCTATTGT TTTTTCCAAT AGGTGGTTAG 61 CAATCGTCTT ACTTTCTAAC TTTTCTTACC TTTTACATTT CAGCAATATA TATATATATT 121 TCAAGGATAT ACCATTCTAA TGTCTGCCCC TAAGAAGATC GTCGTTTTGC CAGGTGACCA 181 CGTTGGTCAA GAAATCACAG CCGAAGCCAT TAAGGTTCTT AAAGCTATTT CTGATGTTCG 241 TTCCAATGTC AAGTTCGATT TCGAAAATCA TTTAATTGGT GGTGCTGCTA TCGATGCTAC 301 AGGTGTTCCA CTTCCAGATG AGGCGCTGGA AGCCTCCAAG AAGGCTGATG CCGTTTTGTT 361 AGGTGCTGTG GGTGGTCCTA AATGGGGTAC CGGTAGTGTT AGACCTGAAC AAGGTTTACT 421 AAAAATCCGT AAAGAACTTC AATTGTACGC CAACTTAAGA CCATGTAACT TTGCATCCGA 481 CTCTCTTTTA GACTTATCTC CAATCAAGCC ACAATTTGCT AAAGGTACTG ACTTCGTTGT 541 TGTCAGAGAA TTAGTGGGAG GTATTTACTT TGGTAAGAGA AAGGAAGACG ATGGTGATGG 601 TGTCGCTTGG GATAGTGAAC AATACACCGT TCCAGAAGTG CAAAGAATCA CAAGAATGGC 661 CGCTTTCATG GCCCTACAAC ATGAGCCACC ATTGCCTATT TGGTCCTTGG GATAAAGCTA 721 ATGTTTTGGC CCTCTTCAAG ATTATGGAGA AAAACTGTGG AGGGAAACCC ATCAAGAACG 781 AATTCCCCTA CATTGAAAGG TTCAAACATC AATTGATTGA TTCTGCCGCC ATGATCCTAG 841 TTTAAGAACC CAACCCACCT AAATGGGTAT TATAATCACC AGCAACATTG TTTGGTGACA 901 TCATCTCCCG ATGAAAGCCT CCCGTTATCC CCAGGTTCCT TTGGGTTTGT TGGCCAATCT 961 GCCGTCCTTT GGCCTCTTTG CCCAGACAAA GAAACACCCG CAATTATGTT TGTTACGAAT 1021 CCATGCCACC GGTTCTGGCT TCCAGATTTG GCCAAAAGAA GTAGCGTCCA ACGCCTACTC 1081 TGGCGCACGT CGATCCTTGG TTCTGCCTGG CCATTGAATG TGCGAAATTG TTCCATTTGA 1141 ACCTTGGTCT GGAAGAAAAG GTATAAGGGC CCCATGTCGA GA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
|---|
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl