Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGFH41

S. pombe expression vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14476_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14476_A6L7a.seq
>06590_14476_A6L7_nmt1Fw_C11_08_ABI24.ab1     1 AGGAAATTTT TTTTAAAATC AGGGAAAACG TAAACTCTCG GCTAACTGGA TGGTTCAAGT    61 CACCCAACGA TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT   121 GTCATTCGGC AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT   181 TGAAGATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT   241 TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC   301 TGGATCCGGA TATCATGAGT AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG   361 TTGAATTAGA TGGTGATGTT AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG   421 ATGCAACATA CGGAAAACTT ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC   481 CGTGGCCAAC ACTTGTCACT ACTTTCTGTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG   541 ATCACATGAA ACAGCATGAC TTTTTCAAGA GTGCCATGCC CGAAGGCTAT GTACAGGAAA   601 GAACTATATT TTTCAAAGAT GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG   661 GTGATACCCT TGTTAATAGA ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA   721 TTCTTGGACA CAAATTGGAA TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA   781 AACAAAAGAA TGGAATCAAA GTTAACTTCA AAATTAGACA CAACATTGAA GATGGGAAGC   841 GTTCAACTAG CAGACCATTA TCAACAAAAT ACTCCCAATT GGCGATGGCC CTGTCCTTTT   901 ACCAGACAAC CATTTACCTG TCCACACAAT CTGGCCCTTT TCGAAAGATC CCAACGAAAA   961 GAGAGACCCA CATGGGTCCC TTCTTTGAAG TTTGGTAACA GCTGCTGGGA ATTACACATG  1021 GGCATGGGAT GAAACTATAC AACCCCATGG ACTACAAGGA CGAACGATGA ACAAACATCA  1081 CTACAATCAC CACTGAGATC TCGAAGTCCG GGCGGCCGCC TAAGCTTAAA CCTTAAGGGC 1141 TCATCAACAA ACGGCA //
Primer: nmt1ter_R Sequence file: RDB14476_A6L7b.seq
>06590_14476_A6L7_nmt1ter_R_D11_11_ABI24.ab1     1 GTTGTGTAGT TACTCATGTA TAAACGTTTT GGGTTCATAT TTTTGGGCCA CGTGGTTTAT    61 AAAAATTCTT AACTACACCA CTCGGTATCC CGCACCCGTC TACGTTTCTA CGATTTCGAG   121 CTAATATTGA GTAAAGTGAT TAGCAAAAAA AATAAAAGTA CTCGTTGTCG GAGATCAAGA   181 ATTTTTCTAT TATCTCATCT AAACCACTTT CTAAAAGCGA AAAACAAAAT CGTAATATGC   241 AGCTTGAATG GGCTTCCATA GTTTGAAAGA AAAACCCTAG CAGTACTGGC AAGGGAGACA   301 TTCCTTTTAC CCTTAGTTAG TTAGCGGCCG CCCGGGCTCG AGATCTCAGT GGTGATGGTG   361 GTGATGTTTG TCATCGTCGT CCTTGTAGTC CATGGGTTTG TATAGTTCAT CCATGCCATG   421 TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG TGGTCTCTCT TTTCGTTGGG   481 ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG TCTGGTAAAA GGACAGGGCC   541 ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT TGAACGCTTC CATCTTCAAT   601 GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT TGTTTGTCTG CCATGATGTA   661 TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA AGAATGTTTC CATCTTCTTT   721 AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA TCACCTTCAA ACTTGACTTC   781 AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA GTTCTTTCCT GTACATAGCC   841 TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG TGATCTGGGT ATCTTGAAAA   901 GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC CACGGAAACA GTAGTTTTTC   961 CAGTAGTGCA AATAAATTTA AACGGTAAGT TTTCCCGTAT GTTGCATCAC CCTTTCACCC  1021 CTCTCCACTG ACAGAAAATT TGTGCCCATT TAACATCACC ATCTACTCAA CAGAATTGGA 1081 CCACTTCCAG TGAAAAAGGT CGTCTCCTTT TAACCTCATG CGAT //
Primer: f1ori_R3 Sequence file: RDB14476_A6L7c.seq
>06590_14476_A6L7_f1ori_R3_F11_17_ABI24.ab1     1 GGAATGGGGC TTGAGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC    61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC   121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA   181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC   241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG   301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC   361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT   421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA   481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG   541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT   601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT   661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG   721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC   781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG   841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT   901 TTATAAAGGC TCATGGGCGT TAAAGAGGAT AGTAAAAAAT TCTACAATTT TTTTAAATTT   961 AATGTCACAA ATTGTCTTTG CGAAATATAA TTTCTAATTA AATTTTCGCT AACTGCTGTT  1021 TTAGTACTAT TACTTTCAAT ATGCCCTTTA TATGCAACTT TAATTCATTG AGGAATGCAA 1081 TATTTTGCAT TCTTCTTGTA CTGAGCGCTT TTTTATATTC CAACTTTAG //
Primer: LEU2pro_F Sequence file: RDB14476_A6L7d.seq
>06590_14476_A6L7_LEU2pro_F_E11_14_ABI24.ab1     1 AATCCTTGGA GGTTGACAAT ATTATTTAAG GACCTATTGT TTTTTCCAAT AGGTGGTTAG    61 CAATCGTCTT ACTTTCTAAC TTTTCTTACC TTTTACATTT CAGCAATATA TATATATATT   121 TCAAGGATAT ACCATTCTAA TGTCTGCCCC TAAGAAGATC GTCGTTTTGC CAGGTGACCA   181 CGTTGGTCAA GAAATCACAG CCGAAGCCAT TAAGGTTCTT AAAGCTATTT CTGATGTTCG   241 TTCCAATGTC AAGTTCGATT TCGAAAATCA TTTAATTGGT GGTGCTGCTA TCGATGCTAC   301 AGGTGTTCCA CTTCCAGATG AGGCGCTGGA AGCCTCCAAG AAGGCTGATG CCGTTTTGTT   361 AGGTGCTGTG GGTGGTCCTA AATGGGGTAC CGGTAGTGTT AGACCTGAAC AAGGTTTACT   421 AAAAATCCGT AAAGAACTTC AATTGTACGC CAACTTAAGA CCATGTAACT TTGCATCCGA   481 CTCTCTTTTA GACTTATCTC CAATCAAGCC ACAATTTGCT AAAGGTACTG ACTTCGTTGT   541 TGTCAGAGAA TTAGTGGGAG GTATTTACTT TGGTAAGAGA AAGGAAGACG ATGGTGATGG   601 TGTCGCTTGG GATAGTGAAC AATACACCGT TCCAGAAGTG CAAAGAATCA CAAGAATGGC   661 CGCTTTCATG GCCCTACAAC ATGAGCCACC ATTGCCTATT TGGTCCTTGG GATAAAGCTA   721 ATGTTTTGGC CCTCTTCAAG ATTATGGAGA AAAACTGTGG AGGGAAACCC ATCAAGAACG   781 AATTCCCCTA CATTGAAAGG TTCAAACATC AATTGATTGA TTCTGCCGCC ATGATCCTAG   841 TTTAAGAACC CAACCCACCT AAATGGGTAT TATAATCACC AGCAACATTG TTTGGTGACA   901 TCATCTCCCG ATGAAAGCCT CCCGTTATCC CCAGGTTCCT TTGGGTTTGT TGGCCAATCT   961 GCCGTCCTTT GGCCTCTTTG CCCAGACAAA GAAACACCCG CAATTATGTT TGTTACGAAT  1021 CCATGCCACC GGTTCTGGCT TCCAGATTTG GCCAAAAGAA GTAGCGTCCA ACGCCTACTC  1081 TGGCGCACGT CGATCCTTGG TTCTGCCTGG CCATTGAATG TGCGAAATTG TTCCATTTGA 1141 ACCTTGGTCT GGAAGAAAAG GTATAAGGGC CCCATGTCGA GA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl