Sequencing and PCR primers for primer information.
S. pombe expression vector with nmt1 promoter
| Catalog number | RDB06589 |
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| Resource name | pGFH1 |
| Clone info. | S. pombe expression vector with nmt1 promoter |
| Vector backbone | pREP1 (Plasmid) |
| Selectable markers | Amp^r |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] Schedule A (GFP-A) [Link] |
| Remarks | ((Additional form)) GFP-A Please use Schedule A (GFP-A) additionally. |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06589 | pGFH1 | DNA solution |
Electronic file
| Electronic file | Sequence RDB06589z.seq from Depositor |
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Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14475_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | RDB14475_A6L7a.seq |
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>06589_14475_A6L7_nmt1Fw_G10_19_ABI24.ab1 1 ATGAATTTTA ATTAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC 241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG 301 GATCCGGATA TCATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT 361 GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT 421 GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG 481 TGGCCAACAC TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT 541 CACATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA 601 ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT 661 GATACCCTTG TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT 721 CTTGGACACA AATTGGAATA CACTATACCT CCACCACATG TATAACATCA TGGCAGACAA 781 ACAAAAGATG ATCCAAAGTT ACTTCCAAAT TAGAACACAA CATGAGATGA GCGTCACTAG 841 CAGACATTAT CAACAAATAC TCATGGGATG GCCTGTCTTT ACGACACATA CTGTTCAACA 901 TCTGCCTTCG AAGAATCCTC ATCGAAAGAG AACACAATGT TCTTCTGGAT TGGAACCTGT 961 GCTGGAATAC ATGCTGGATA CTTCACCTGT ATGACGCCAA GTGCATCCGA TGACGT // |
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| Primer: nmt1ter_R | RDB14475_A6L7b.seq |
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>06589_14475_A6L7_nmt1ter_R_H10_22_ABI24.ab1 1 GACCTTCCTA GAAACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT 61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG 121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA 181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT 241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA 301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA GTGGTGATGG 361 TGGTGATGTT TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA 421 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG 481 GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG 541 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA 601 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG 661 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT 721 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT 781 TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAG 841 CCTTCGGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA 901 AAAGCATTGA ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCCACAGA ACAGGTAGTT 961 TTTCCAGTAG TGCAAATAAT TTAAGGGGTA AGGTTTTCCG TATGTTTGCA ATCCACCTTT 1021 CACCCTTCTC ACCTGACCAG AAAATTGTGC CCCATTTAAC ATCAGCATCT AAATCCAACA 1081 AGATGGACCA CTTCCAGGTG AAGGTCTCTC CTCTTTTACC TCAGTGTA // |
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| Primer: f1ori_R3 | RDB14475_A6L7c.seq |
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>06589_14475_A6L7_f1ori_R3_B11_05_ABI24.ab1 1 GGATGGGTCT TGCGTATGCG GTGTGATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC 181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCC AACCTTCCAA 241 TTCATTAAAT CGAGTCCTCC ATCAGATGAT TTACAAGGTT TTGCATAGAA TCCCTAAGTA 301 AGAGAAGTTG TTAGTGATTT CTTCAAGTTT ACTAAAATTT ACATACAAAT GGATGATCTC 361 TTCTCCATTG TTTCCTTTCT TCTTGCAAAC GCGTTTTACA AAGAGATGAC ATGCTATTTT 421 CTGATTATTT TTTTCTATAG TTTTCTATTT TGTACGTCCT TGGGAGTCCG AAATGTAAAA 481 TCGGGGTATG GTTAGTAGCG GCAGCGTTCA TTATGGTATA TAAAAGATAA AAAGTAGGGG 541 CAAGCGAGAC AGAATGGGAT ACAAGGGCAT CGTCTATAAT TATAGCTAAA AATTGTATTT 601 TAATTTGTAT TTTTTGTAAT TTTATTTCTC ATTGTTTTAC TTAAAATGAA TGCGAATTAG 661 AGAAAACTTC AACGAAATGT CAAAATAAGC TCAGCAAAAT ATACAATTTT AGGGAAAGCG 721 ATCAGCAACT TATTCTCCGT TGTAGATTCT TTTTCTTCAT AAATTCCAAC AATGTAACTG 781 CCAATTGATT TTTCTCTAGA TGCTAATAAA TTGAAACTAA TGAATATAAT AACACAGGTT 841 TTGATAAATT GTAAACTCTT ACTCAAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTT 901 ATAAAGGCTC ATGGCGTTTA AAGAGGGATA GTAAAAAATT CTACAATTTT TTTAATTTAA 961 TGTCACAATT TGTCTTTGCG AAATATAATT CTATTAAATT TTTCGCTAAC TGCTGTTTTA 1021 GTACTATTAC TTTCATATGC CCTATATGCA AACTTTGATC ATGAGATGCA ATATTTTTGC 1081 ATCATCTGTA CTGAAGCGCT TTTTTAACTC AATTTTTAGG // |
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| Primer: LEU2pro_F | RDB14475_A6L7d.seq |
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>06589_14475_A6L7_LEU2pro_F_A11_02_ABI24.ab1 1 CATTCTTGCA GTGGCATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT 61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA 121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT 181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC 241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT 301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT 361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA 421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT 481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC 541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC 601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT 661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT 721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT 781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA 841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GGTGACATCA TCTCCGATGA 901 AGCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTT GGCCTCTTTG 961 CCAGACAAGA AACACCGCAA TTTGGGTTTG TACGAAACCA TGCCACGATT CTGCTCAGAT 1021 TTTGCCCAAA GAATAGTCAA CCTATCGGCC ACTATCTTGG TCTGCTGCAA TGATGATGAA 1081 TGTCAATGAA CCTTGGCCTG GAAGAAGTTA AGGCCAATGT GGA // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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2018.12.10
GNP_filter3_RDBDEP_html_181012.pl