Resource data sheet
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pGFH1

S. pombe expression vector with nmt1 promoter

Catalog number RDB06589
Resource name pGFH1
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06589 pGFH1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06589z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14475_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14475_A6L7a.seq
>06589_14475_A6L7_nmt1Fw_G10_19_ABI24.ab1
    1 ATGAATTTTA ATTAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
  241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
  301 GATCCGGATA TCATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT
  361 GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT
  421 GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG
  481 TGGCCAACAC TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT
  541 CACATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA
  601 ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT
  661 GATACCCTTG TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT
  721 CTTGGACACA AATTGGAATA CACTATACCT CCACCACATG TATAACATCA TGGCAGACAA
  781 ACAAAAGATG ATCCAAAGTT ACTTCCAAAT TAGAACACAA CATGAGATGA GCGTCACTAG
  841 CAGACATTAT CAACAAATAC TCATGGGATG GCCTGTCTTT ACGACACATA CTGTTCAACA
  901 TCTGCCTTCG AAGAATCCTC ATCGAAAGAG AACACAATGT TCTTCTGGAT TGGAACCTGT
961 GCTGGAATAC ATGCTGGATA CTTCACCTGT ATGACGCCAA GTGCATCCGA TGACGT
//
Primer: nmt1ter_R RDB14475_A6L7b.seq
>06589_14475_A6L7_nmt1ter_R_H10_22_ABI24.ab1
    1 GACCTTCCTA GAAACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT
   61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG
  121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA
  181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT
  241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA
  301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA GTGGTGATGG
  361 TGGTGATGTT TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA
  421 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG
  481 GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG
  541 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA
  601 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG
  661 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT
  721 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT
  781 TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAG
  841 CCTTCGGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA
  901 AAAGCATTGA ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCCACAGA ACAGGTAGTT
  961 TTTCCAGTAG TGCAAATAAT TTAAGGGGTA AGGTTTTCCG TATGTTTGCA ATCCACCTTT
 1021 CACCCTTCTC ACCTGACCAG AAAATTGTGC CCCATTTAAC ATCAGCATCT AAATCCAACA
1081 AGATGGACCA CTTCCAGGTG AAGGTCTCTC CTCTTTTACC TCAGTGTA
//
Primer: f1ori_R3 RDB14475_A6L7c.seq
>06589_14475_A6L7_f1ori_R3_B11_05_ABI24.ab1
    1 GGATGGGTCT TGCGTATGCG GTGTGATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
   61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
  121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
  181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCC AACCTTCCAA
  241 TTCATTAAAT CGAGTCCTCC ATCAGATGAT TTACAAGGTT TTGCATAGAA TCCCTAAGTA
  301 AGAGAAGTTG TTAGTGATTT CTTCAAGTTT ACTAAAATTT ACATACAAAT GGATGATCTC
  361 TTCTCCATTG TTTCCTTTCT TCTTGCAAAC GCGTTTTACA AAGAGATGAC ATGCTATTTT
  421 CTGATTATTT TTTTCTATAG TTTTCTATTT TGTACGTCCT TGGGAGTCCG AAATGTAAAA
  481 TCGGGGTATG GTTAGTAGCG GCAGCGTTCA TTATGGTATA TAAAAGATAA AAAGTAGGGG
  541 CAAGCGAGAC AGAATGGGAT ACAAGGGCAT CGTCTATAAT TATAGCTAAA AATTGTATTT
  601 TAATTTGTAT TTTTTGTAAT TTTATTTCTC ATTGTTTTAC TTAAAATGAA TGCGAATTAG
  661 AGAAAACTTC AACGAAATGT CAAAATAAGC TCAGCAAAAT ATACAATTTT AGGGAAAGCG
  721 ATCAGCAACT TATTCTCCGT TGTAGATTCT TTTTCTTCAT AAATTCCAAC AATGTAACTG
  781 CCAATTGATT TTTCTCTAGA TGCTAATAAA TTGAAACTAA TGAATATAAT AACACAGGTT
  841 TTGATAAATT GTAAACTCTT ACTCAAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTT
  901 ATAAAGGCTC ATGGCGTTTA AAGAGGGATA GTAAAAAATT CTACAATTTT TTTAATTTAA
  961 TGTCACAATT TGTCTTTGCG AAATATAATT CTATTAAATT TTTCGCTAAC TGCTGTTTTA
 1021 GTACTATTAC TTTCATATGC CCTATATGCA AACTTTGATC ATGAGATGCA ATATTTTTGC
1081 ATCATCTGTA CTGAAGCGCT TTTTTAACTC AATTTTTAGG
//
Primer: LEU2pro_F RDB14475_A6L7d.seq
>06589_14475_A6L7_LEU2pro_F_A11_02_ABI24.ab1
    1 CATTCTTGCA GTGGCATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
   61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GGTGACATCA TCTCCGATGA
  901 AGCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTT GGCCTCTTTG
  961 CCAGACAAGA AACACCGCAA TTTGGGTTTG TACGAAACCA TGCCACGATT CTGCTCAGAT
 1021 TTTGCCCAAA GAATAGTCAA CCTATCGGCC ACTATCTTGG TCTGCTGCAA TGATGATGAA
1081 TGTCAATGAA CCTTGGCCTG GAAGAAGTTA AGGCCAATGT GGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl