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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGFH1

S. pombe expression vector with nmt1 promoter

Catalog number RDB06589
Resource name pGFH1
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06589 pGFH1 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06589z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14475_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14475_A6L7a.seq check
            >06589_14475_A6L7_nmt1Fw_G10_19_ABI24.ab1
                1 ATGAATTTTA ATTAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
               61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
              121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
              181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
              241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
              301 GATCCGGATA TCATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT
              361 GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT
              421 GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG
              481 TGGCCAACAC TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT
              541 CACATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA
              601 ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT
              661 GATACCCTTG TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT
              721 CTTGGACACA AATTGGAATA CACTATACCT CCACCACATG TATAACATCA TGGCAGACAA
              781 ACAAAAGATG ATCCAAAGTT ACTTCCAAAT TAGAACACAA CATGAGATGA GCGTCACTAG
              841 CAGACATTAT CAACAAATAC TCATGGGATG GCCTGTCTTT ACGACACATA CTGTTCAACA
              901 TCTGCCTTCG AAGAATCCTC ATCGAAAGAG AACACAATGT TCTTCTGGAT TGGAACCTGT
            961 GCTGGAATAC ATGCTGGATA CTTCACCTGT ATGACGCCAA GTGCATCCGA TGACGT
            //
            Primer: nmt1ter_R
            Sequence file: RDB14475_A6L7b.seq check
            >06589_14475_A6L7_nmt1ter_R_H10_22_ABI24.ab1
                1 GACCTTCCTA GAAACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT
               61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG
              121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA
              181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT
              241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA
              301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA GTGGTGATGG
              361 TGGTGATGTT TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA
              421 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG
              481 GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG
              541 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA
              601 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG
              661 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT
              721 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT
              781 TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAG
              841 CCTTCGGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA
              901 AAAGCATTGA ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCCACAGA ACAGGTAGTT
              961 TTTCCAGTAG TGCAAATAAT TTAAGGGGTA AGGTTTTCCG TATGTTTGCA ATCCACCTTT
             1021 CACCCTTCTC ACCTGACCAG AAAATTGTGC CCCATTTAAC ATCAGCATCT AAATCCAACA
            1081 AGATGGACCA CTTCCAGGTG AAGGTCTCTC CTCTTTTACC TCAGTGTA
            //
            Primer: f1ori_R3
            Sequence file: RDB14475_A6L7c.seq check
            >06589_14475_A6L7_f1ori_R3_B11_05_ABI24.ab1
                1 GGATGGGTCT TGCGTATGCG GTGTGATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
               61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
              121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
              181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCC AACCTTCCAA
              241 TTCATTAAAT CGAGTCCTCC ATCAGATGAT TTACAAGGTT TTGCATAGAA TCCCTAAGTA
              301 AGAGAAGTTG TTAGTGATTT CTTCAAGTTT ACTAAAATTT ACATACAAAT GGATGATCTC
              361 TTCTCCATTG TTTCCTTTCT TCTTGCAAAC GCGTTTTACA AAGAGATGAC ATGCTATTTT
              421 CTGATTATTT TTTTCTATAG TTTTCTATTT TGTACGTCCT TGGGAGTCCG AAATGTAAAA
              481 TCGGGGTATG GTTAGTAGCG GCAGCGTTCA TTATGGTATA TAAAAGATAA AAAGTAGGGG
              541 CAAGCGAGAC AGAATGGGAT ACAAGGGCAT CGTCTATAAT TATAGCTAAA AATTGTATTT
              601 TAATTTGTAT TTTTTGTAAT TTTATTTCTC ATTGTTTTAC TTAAAATGAA TGCGAATTAG
              661 AGAAAACTTC AACGAAATGT CAAAATAAGC TCAGCAAAAT ATACAATTTT AGGGAAAGCG
              721 ATCAGCAACT TATTCTCCGT TGTAGATTCT TTTTCTTCAT AAATTCCAAC AATGTAACTG
              781 CCAATTGATT TTTCTCTAGA TGCTAATAAA TTGAAACTAA TGAATATAAT AACACAGGTT
              841 TTGATAAATT GTAAACTCTT ACTCAAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTT
              901 ATAAAGGCTC ATGGCGTTTA AAGAGGGATA GTAAAAAATT CTACAATTTT TTTAATTTAA
              961 TGTCACAATT TGTCTTTGCG AAATATAATT CTATTAAATT TTTCGCTAAC TGCTGTTTTA
             1021 GTACTATTAC TTTCATATGC CCTATATGCA AACTTTGATC ATGAGATGCA ATATTTTTGC
            1081 ATCATCTGTA CTGAAGCGCT TTTTTAACTC AATTTTTAGG
            //
            Primer: LEU2pro_F
            Sequence file: RDB14475_A6L7d.seq check
            >06589_14475_A6L7_LEU2pro_F_A11_02_ABI24.ab1
                1 CATTCTTGCA GTGGCATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
               61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
              121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
              181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
              241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
              301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
              361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
              421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
              481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
              541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
              601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
              661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
              721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
              781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
              841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GGTGACATCA TCTCCGATGA
              901 AGCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTT GGCCTCTTTG
              961 CCAGACAAGA AACACCGCAA TTTGGGTTTG TACGAAACCA TGCCACGATT CTGCTCAGAT
             1021 TTTGCCCAAA GAATAGTCAA CCTATCGGCC ACTATCTTGG TCTGCTGCAA TGATGATGAA
            1081 TGTCAATGAA CCTTGGCCTG GAAGAAGTTA AGGCCAATGT GGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl