Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGFH1

S. pombe expression vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Schedule A (GFP-A) [Link]
Remarks	Please use Schedule A (GFP-A) additionally.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] Schedule A（GFP-A, 英語版のみ） [Link]
備考	Schedule A（GFP-A, 英語版のみ）が追加で必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14475_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14475_A6L7a.seq
>06589_14475_A6L7_nmt1Fw_G10_19_ABI24.ab1     1 ATGAATTTTA ATTAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA    61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT   121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA   181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC   241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG   301 GATCCGGATA TCATGAGTAA AGGAGAAGAA CTTTTCACTG GAGTTGTCCC AATTCTTGTT   361 GAATTAGATG GTGATGTTAA TGGGCACAAA TTTTCTGTCA GTGGAGAGGG TGAAGGTGAT   421 GCAACATACG GAAAACTTAC CCTTAAATTT ATTTGCACTA CTGGAAAACT ACCTGTTCCG   481 TGGCCAACAC TTGTCACTAC TTTCTGTTAT GGTGTTCAAT GCTTTTCAAG ATACCCAGAT   541 CACATGAAAC AGCATGACTT TTTCAAGAGT GCCATGCCCG AAGGCTATGT ACAGGAAAGA   601 ACTATATTTT TCAAAGATGA CGGGAACTAC AAGACACGTG CTGAAGTCAA GTTTGAAGGT   661 GATACCCTTG TTAATAGAAT CGAGTTAAAA GGTATTGATT TTAAAGAAGA TGGAAACATT   721 CTTGGACACA AATTGGAATA CACTATACCT CCACCACATG TATAACATCA TGGCAGACAA   781 ACAAAAGATG ATCCAAAGTT ACTTCCAAAT TAGAACACAA CATGAGATGA GCGTCACTAG   841 CAGACATTAT CAACAAATAC TCATGGGATG GCCTGTCTTT ACGACACATA CTGTTCAACA   901 TCTGCCTTCG AAGAATCCTC ATCGAAAGAG AACACAATGT TCTTCTGGAT TGGAACCTGT 961 GCTGGAATAC ATGCTGGATA CTTCACCTGT ATGACGCCAA GTGCATCCGA TGACGT //
Primer: nmt1ter_R Sequence file: RDB14475_A6L7b.seq
>06589_14475_A6L7_nmt1ter_R_H10_22_ABI24.ab1     1 GACCTTCCTA GAAACTCATG TATAAACGTT TTGGGTTCAT ATTTTTGGGC CACGTGGTTT    61 ATAAAAATTC TTAACTACAC CACTCGGTAT CCCGCACCCG TCTACGTTTC TACGATTTCG   121 AGCTAATATT GAGTAAAGTG ATTAGCAAAA AAAATAAAAG TACTCGTTGT CGGAGATCAA   181 GAATTTTTCT ATTATCTCAT CTAAACCACT TTCTAAAAGC GAAAAACAAA ATCGTAATAT   241 GCAGCTTGAA TGGGCTTCCA TAGTTTGAAA GAAAAACCCT AGCAGTACTG GCAAGGGAGA   301 CATTCCTTTT ACCCTTAGTT AGTTAGCGGC CGCCCGGGCT CGAGATCTCA GTGGTGATGG   361 TGGTGATGTT TGTCATCGTC GTCCTTGTAG TCCATGGGTT TGTATAGTTC ATCCATGCCA   421 TGTGTAATCC CAGCAGCTGT TACAAACTCA AGAAGGACCA TGTGGTCTCT CTTTTCGTTG   481 GGATCTTTCG AAAGGGCAGA TTGTGTGGAC AGGTAATGGT TGTCTGGTAA AAGGACAGGG   541 CCATCGCCAA TTGGAGTATT TTGTTGATAA TGGTCTGCTA GTTGAACGCT TCCATCTTCA   601 ATGTTGTGTC TAATTTTGAA GTTAACTTTG ATTCCATTCT TTTGTTTGTC TGCCATGATG   661 TATACATTGT GTGAGTTATA GTTGTATTCC AATTTGTGTC CAAGAATGTT TCCATCTTCT   721 TTAAAATCAA TACCTTTTAA CTCGATTCTA TTAACAAGGG TATCACCTTC AAACTTGACT   781 TCAGCACGTG TCTTGTAGTT CCCGTCATCT TTGAAAAATA TAGTTCTTTC CTGTACATAG   841 CCTTCGGGGC ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA   901 AAAGCATTGA ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCCACAGA ACAGGTAGTT   961 TTTCCAGTAG TGCAAATAAT TTAAGGGGTA AGGTTTTCCG TATGTTTGCA ATCCACCTTT  1021 CACCCTTCTC ACCTGACCAG AAAATTGTGC CCCATTTAAC ATCAGCATCT AAATCCAACA 1081 AGATGGACCA CTTCCAGGTG AAGGTCTCTC CTCTTTTACC TCAGTGTA //
Primer: f1ori_R3 Sequence file: RDB14475_A6L7c.seq
>06589_14475_A6L7_f1ori_R3_B11_05_ABI24.ab1     1 GGATGGGTCT TGCGTATGCG GTGTGATACC GCACAGATGC GTAAGGAGAA AATACCGCAT    61 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC   121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC   181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAATTCC AACCTTCCAA   241 TTCATTAAAT CGAGTCCTCC ATCAGATGAT TTACAAGGTT TTGCATAGAA TCCCTAAGTA   301 AGAGAAGTTG TTAGTGATTT CTTCAAGTTT ACTAAAATTT ACATACAAAT GGATGATCTC   361 TTCTCCATTG TTTCCTTTCT TCTTGCAAAC GCGTTTTACA AAGAGATGAC ATGCTATTTT   421 CTGATTATTT TTTTCTATAG TTTTCTATTT TGTACGTCCT TGGGAGTCCG AAATGTAAAA   481 TCGGGGTATG GTTAGTAGCG GCAGCGTTCA TTATGGTATA TAAAAGATAA AAAGTAGGGG   541 CAAGCGAGAC AGAATGGGAT ACAAGGGCAT CGTCTATAAT TATAGCTAAA AATTGTATTT   601 TAATTTGTAT TTTTTGTAAT TTTATTTCTC ATTGTTTTAC TTAAAATGAA TGCGAATTAG   661 AGAAAACTTC AACGAAATGT CAAAATAAGC TCAGCAAAAT ATACAATTTT AGGGAAAGCG   721 ATCAGCAACT TATTCTCCGT TGTAGATTCT TTTTCTTCAT AAATTCCAAC AATGTAACTG   781 CCAATTGATT TTTCTCTAGA TGCTAATAAA TTGAAACTAA TGAATATAAT AACACAGGTT   841 TTGATAAATT GTAAACTCTT ACTCAAAAAA AAGAATGTTA AAACTAATTT AACCTTTTTT   901 ATAAAGGCTC ATGGCGTTTA AAGAGGGATA GTAAAAAATT CTACAATTTT TTTAATTTAA   961 TGTCACAATT TGTCTTTGCG AAATATAATT CTATTAAATT TTTCGCTAAC TGCTGTTTTA  1021 GTACTATTAC TTTCATATGC CCTATATGCA AACTTTGATC ATGAGATGCA ATATTTTTGC 1081 ATCATCTGTA CTGAAGCGCT TTTTTAACTC AATTTTTAGG //
Primer: LEU2pro_F Sequence file: RDB14475_A6L7d.seq
>06589_14475_A6L7_LEU2pro_F_A11_02_ABI24.ab1     1 CATTCTTGCA GTGGCATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT    61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA   121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT   181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC   241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT   301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT   361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA   421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT   481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC   541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC   601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT   661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT   721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT   781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA   841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GGTGACATCA TCTCCGATGA   901 AGCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTT GGCCTCTTTG   961 CCAGACAAGA AACACCGCAA TTTGGGTTTG TACGAAACCA TGCCACGATT CTGCTCAGAT  1021 TTTGCCCAAA GAATAGTCAA CCTATCGGCC ACTATCTTGG TCTGCTGCAA TGATGATGAA 1081 TGTCAATGAA CCTTGGCCTG GAAGAAGTTA AGGCCAATGT GGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl