Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pFFH81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06588
Resource name pFFH81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06588 pFFH81 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06588z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14499_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14499_A6L7a.seq check
            >06588_14499_A6L7_nmt1Fw_D10_10_ABI24.ab1
                1 ATAATTTTTA TTAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
               61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
              121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
              181 TGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
              241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCGG
              301 ATATCATGGA TTATAAGGAT GATGACGATA AACCCATGGA CTACAAGGAC GACGATGACA
              361 AACATCACCA CCATCACCAC TGAGATCTCG AGCCCGGGCG GCCGCTAACT AACTAAGGGT
              421 AAAAGGAATG TCTCCCTTGC CAGTACTGCT AGGGTTTTTC TTTCAAACTA TGGAAGCCCA
              481 TTCAAGCTGC ATATTACGAT TTTGTTTTTC GCTTTTAGAA AGTGGTTTAG ATGAGATAAT
              541 AGAAAAATTC TTGATCTCCG ACAACGAGTA CTTTTATTTT TTTTGCTAAT CACTTTACTC
              601 AATATTAGCT CGAAATCGTA GAAACGTAGA CGGGTGCGGG ATACCGAGTG GTGTAGTTAA
              661 GAATTTTTAT AAACCACGTG GCCCAAAAAT ATGAACCCAA AACGTTTATA CATGAGTATA
              721 CTTTAAGAAG GCTATACCCC TTCGTGTTAG ATGTAGTTTT AGCTACCCAA CCCGAGTCTA
              781 TGAGCTTGAC TTCAGATGTA GAAGGCATTA AATCGTTTTG AATATTAATT AAAAAAACGA
              841 TGAAAATTAA ATATTTAAAA GCAATCATAC GCTGAAAATT TAGTGCTGTG GCTAATCCTT
              901 CAACATGGAA ATGCCATAAA AGTGACTTTG ACAAAAAAAA AAAAGTATAT ACAGGTAGTA
              961 AACTCATCTA CTTCATTGAC TTTGTTTACA GCATGTGAAG GAGATATTAT TTGCTAATCG
             1021 TAGTTACATT CAATAAGTAT ACTATTGGAA ATCCGACAGA TGGTCGCATG CATGAAGAGG
            1081 CCATTTTGCC TTTGGGGGAG AATTAGCTTC
            //
            Primer: f1ori_R3
            Sequence file: RDB14499_A6L7b.seq check
            >06588_14499_A6L7_f1ori_R3_F10_16_ABI24.ab1
                1 GCAGTGCTGC TTGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
               61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
              121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
              181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
              241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
              301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
              361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
              421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
              481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
              541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
              601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
              661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
              721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
              781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
              841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAAGAATGT TAAAACTAAT TTAACCCTTT
              901 TTTATAAAGG CTCATGGGCG TTTAAAGAGG GATAGTAAAA ATTTCTACAA TTTTTTTAAT
              961 TTAAATGTCA CAATTTGTCT TTGCGAATAT AATTCTATTA ATTTTCGCTA ACTGCTGTTT
             1021 TAGTACTATT TACTTTCCAT ATGCCTTATA TGCACTTTAA TCATGAGATG CATATTTTTG
            1081 CATCTTCCTG TACTGGAACG CTTTTTTAAT CCAATTTAAG TAACA
            //
            Primer: LEU2pro_F
            Sequence file: RDB14499_A6L7c.seq check
            >06588_14499_A6L7_LEU2pro_F_E10_13_ABI24.ab1
                1 TATCATTTCA GTTGAAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA
               61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC
              121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG
              181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT
              241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG
              301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG
              361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA
              421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT
              481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG
              541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG
              601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG
              661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAGCTAATG
              721 TTTTGGCCTC TTCAAGATTA TGGAGAAAAA CTGTGGAGGA AACCATCAAG AACGAATTCC
              781 CTACATTGAA GGTTCAACAT CAATTGATTG ATTCTGCCGC CATGATCCTA GTTAAGAACC
              841 CAACCCACCT AAATGGTATT ATAATCACCA GCAACATGTT TGGTGACATC ATCTCCGATG
              901 AAGCCCTCCG TTATCCCAGG TTCCTTGGGT TTGTTGCCAT CTGCGTCCTT GGCCCTCTTT
              961 GCCAGACAAG AACACCGCAT TGGGTTTGTA CGAAACCATG CCACGGTTCT GCTCCAGATT
             1021 TGCCAAAGAT AAAGTCAACC CTATCGCCAC TATCTGTCTG CCTGCAATGA TGATGAATGT
            1081 CATGGACTTG CCTGGAAAGA AGGTAAGGGG TCCAAATGTG A
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl