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pFFH81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06588
Resource name pFFH81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06588 pFFH81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06588z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14499_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14499_A6L7a.seq
>06588_14499_A6L7_nmt1Fw_D10_10_ABI24.ab1
    1 ATAATTTTTA TTAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
  241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCGG
  301 ATATCATGGA TTATAAGGAT GATGACGATA AACCCATGGA CTACAAGGAC GACGATGACA
  361 AACATCACCA CCATCACCAC TGAGATCTCG AGCCCGGGCG GCCGCTAACT AACTAAGGGT
  421 AAAAGGAATG TCTCCCTTGC CAGTACTGCT AGGGTTTTTC TTTCAAACTA TGGAAGCCCA
  481 TTCAAGCTGC ATATTACGAT TTTGTTTTTC GCTTTTAGAA AGTGGTTTAG ATGAGATAAT
  541 AGAAAAATTC TTGATCTCCG ACAACGAGTA CTTTTATTTT TTTTGCTAAT CACTTTACTC
  601 AATATTAGCT CGAAATCGTA GAAACGTAGA CGGGTGCGGG ATACCGAGTG GTGTAGTTAA
  661 GAATTTTTAT AAACCACGTG GCCCAAAAAT ATGAACCCAA AACGTTTATA CATGAGTATA
  721 CTTTAAGAAG GCTATACCCC TTCGTGTTAG ATGTAGTTTT AGCTACCCAA CCCGAGTCTA
  781 TGAGCTTGAC TTCAGATGTA GAAGGCATTA AATCGTTTTG AATATTAATT AAAAAAACGA
  841 TGAAAATTAA ATATTTAAAA GCAATCATAC GCTGAAAATT TAGTGCTGTG GCTAATCCTT
  901 CAACATGGAA ATGCCATAAA AGTGACTTTG ACAAAAAAAA AAAAGTATAT ACAGGTAGTA
  961 AACTCATCTA CTTCATTGAC TTTGTTTACA GCATGTGAAG GAGATATTAT TTGCTAATCG
 1021 TAGTTACATT CAATAAGTAT ACTATTGGAA ATCCGACAGA TGGTCGCATG CATGAAGAGG
1081 CCATTTTGCC TTTGGGGGAG AATTAGCTTC
//
Primer: f1ori_R3 RDB14499_A6L7b.seq
>06588_14499_A6L7_f1ori_R3_F10_16_ABI24.ab1
    1 GCAGTGCTGC TTGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
  241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
  301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
  361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
  421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
  481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
  541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
  601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
  661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
  721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
  781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
  841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAAGAATGT TAAAACTAAT TTAACCCTTT
  901 TTTATAAAGG CTCATGGGCG TTTAAAGAGG GATAGTAAAA ATTTCTACAA TTTTTTTAAT
  961 TTAAATGTCA CAATTTGTCT TTGCGAATAT AATTCTATTA ATTTTCGCTA ACTGCTGTTT
 1021 TAGTACTATT TACTTTCCAT ATGCCTTATA TGCACTTTAA TCATGAGATG CATATTTTTG
1081 CATCTTCCTG TACTGGAACG CTTTTTTAAT CCAATTTAAG TAACA
//
Primer: LEU2pro_F RDB14499_A6L7c.seq
>06588_14499_A6L7_LEU2pro_F_E10_13_ABI24.ab1
    1 TATCATTTCA GTTGAAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA
   61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC
  121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG
  181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT
  241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG
  301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG
  361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA
  421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT
  481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG
  541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG
  601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG
  661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAGCTAATG
  721 TTTTGGCCTC TTCAAGATTA TGGAGAAAAA CTGTGGAGGA AACCATCAAG AACGAATTCC
  781 CTACATTGAA GGTTCAACAT CAATTGATTG ATTCTGCCGC CATGATCCTA GTTAAGAACC
  841 CAACCCACCT AAATGGTATT ATAATCACCA GCAACATGTT TGGTGACATC ATCTCCGATG
  901 AAGCCCTCCG TTATCCCAGG TTCCTTGGGT TTGTTGCCAT CTGCGTCCTT GGCCCTCTTT
  961 GCCAGACAAG AACACCGCAT TGGGTTTGTA CGAAACCATG CCACGGTTCT GCTCCAGATT
 1021 TGCCAAAGAT AAAGTCAACC CTATCGCCAC TATCTGTCTG CCTGCAATGA TGATGAATGT
1081 CATGGACTTG CCTGGAAAGA AGGTAAGGGG TCCAAATGTG A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

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