Sequencing and PCR primers for primer information.
S. pombe expression vector with nmt1 promoter
| Catalog number | RDB06586 |
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| Resource name | pFFH1 |
| Clone info. | S. pombe expression vector with nmt1 promoter |
| Vector backbone | pREP1 (Plasmid) |
| Selectable markers | Amp^r |
| Depositor | Yoshida, Minoru | Matsuyama, Akihisa | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06586 | pFFH1 | DNA solution |
Electronic file
| Electronic file | Sequence RDB06586z.seq from Depositor |
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Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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User reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14497_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: nmt1Fw | RDB14497_A6L7a.seq |
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>06586_14497_A6L7_nmt1Fw_F09_18_ABI24.ab1 1 TAGAAATTTT ATTAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA 61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT 121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA 181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC 241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG 301 GATCCGGATA TCATGGATTA TAAGGATGAT GACGATAAAC CCATGGACTA CAAGGACGAC 361 GATGACAAAC ATCACCACCA TCACCACTGA GATCTCGAGC CCGGGCGGCC GCTAACTAAC 421 TAAGGGTAAA AGGAATGTCT CCCTTGCCAG TACTGCTAGG GTTTTTCTTT CAAACTATGG 481 AAGCCCATTC AAGCTGCATA TTACGATTTT GTTTTTCGCT TTTAGAAAGT GGTTTAGATG 541 AGATAATAGA AAAATTCTTG ATCTCCGACA ACGAGTACTT TTATTTTTTT TGCTAATCAC 601 TTTACTCAAT ATTAGCTCGA AATCGTAGAA ACGTAGACGG GTGCGGGATA CCGAGTGGTG 661 TAGTTAAGAA TTTTTATAAA CCACGTGGCC CAAAAATATG AACCCAAAAC GTTTATACAT 721 GAGTATACTT TAAGAAGGCT ATACCCCTTC GTGTTAGATG TAGTTTTAGC TACCCAACCC 781 GAGTCTATGA GCTTGACTTC AGATGTAGAA GGCATTAAAT CGTTTTGAAT ATTAATTAAA 841 AAACGATGAA AATTAAATAT TTAAAAGCAA TCATACGCTG AAAATTTAGT GCTGTGGCTA 901 ATCCTTCAAC ATGGAAATGC CATAAAAGTG ACTTTGACAA AAAAAAAAAG TATATACAGG 961 TAGTAAACTC ATCTACTTCA TTTGACTTTG TTTACAGCAT GTGGGATGAG GAAATATTTA 1021 TTTGCTAAAT CGTAGGTTTA ACATTTCAAT AAGTTAATTA CTATTGAAAA TCGACAGGAT 1081 GCGCATGCAA TGGAAAAAGA GGCCATTTTT GCCCTTTTGC G // |
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| Primer: f1ori_R3 | RDB14497_A6L7b.seq |
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>06586_14497_A6L7_f1ori_R3_H09_24_ABI24.ab1 1 GCAGTGGCTT CTTGGAGTAT GCGGTGTGAT ACCGCACAGA TGCGTAAGGA GAAAATACCG 61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC 121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT 181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC 241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA 301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT 361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT 421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA 481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG 541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA 601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT 661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA 721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA 781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG 841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAAGAATG TTAAAACTAA TTTAACCTTT 901 TTATAAAGGC TCATGGCGTT TAAAGAGGGA TAGTAAAAAT TTCTACAATT TTTTAATTTA 961 ATGTCACAAT TTGTCTTGCG AATATAAATT CTATTAATTT TCGCTAACCT GCCTGTTTTA 1021 GTACTATTAC TTTCAATATG CCTTATATGC AACTTTAAAT CATGAGGATT GCAATTATTT 1081 TGCATCCTTT CCTTGTACCT GAGCGCTTTT TATCAATTTT AAAG // |
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| Primer: LEU2pro_F | RDB14497_A6L7c.seq |
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>06586_14497_A6L7_LEU2pro_F_G09_21_ABI24.ab1 1 AACATGCAGT GAATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG 61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATTTCAAGG 121 ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG 181 TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA 241 TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT 301 TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT TGTTAGGTGC 361 TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT 421 CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT 481 TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG 541 AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC 601 TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT 661 CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT 721 GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AAATTCCCTA 781 CATTGAAGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA GACCCCACCC 841 ACCTAATGGT ATATAATCAC CAGCAACATG TTTGGTGACA TCATCTCGAT GAGGCCTCCG 901 TTATCCAGTC CTGGGTTTGT GGCATCTGCG GTCCTTGGCC TCTTTGGCAG AACAAGAAAC 961 ACCCGCCATT TGTTGTACCG ACATGCCACG TCTGCTCCGA TGGCAGATAG GTCACTATCG 1021 GCAATCTGTC CGCGCACAGT AGTGATTTGC CATG // |
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Please visit Sequencing and PCR primers for primer information.
References
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. |
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2018.12.10
GNP_filter3_RDBDEP_html_181012.pl