Resource data sheet
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pFFH1

S. pombe expression vector with nmt1 promoter

Catalog number RDB06586
Resource name pFFH1
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP1 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06586 pFFH1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06586z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14497_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14497_A6L7a.seq
>06586_14497_A6L7_nmt1Fw_F09_18_ABI24.ab1
    1 TAGAAATTTT ATTAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
  241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
  301 GATCCGGATA TCATGGATTA TAAGGATGAT GACGATAAAC CCATGGACTA CAAGGACGAC
  361 GATGACAAAC ATCACCACCA TCACCACTGA GATCTCGAGC CCGGGCGGCC GCTAACTAAC
  421 TAAGGGTAAA AGGAATGTCT CCCTTGCCAG TACTGCTAGG GTTTTTCTTT CAAACTATGG
  481 AAGCCCATTC AAGCTGCATA TTACGATTTT GTTTTTCGCT TTTAGAAAGT GGTTTAGATG
  541 AGATAATAGA AAAATTCTTG ATCTCCGACA ACGAGTACTT TTATTTTTTT TGCTAATCAC
  601 TTTACTCAAT ATTAGCTCGA AATCGTAGAA ACGTAGACGG GTGCGGGATA CCGAGTGGTG
  661 TAGTTAAGAA TTTTTATAAA CCACGTGGCC CAAAAATATG AACCCAAAAC GTTTATACAT
  721 GAGTATACTT TAAGAAGGCT ATACCCCTTC GTGTTAGATG TAGTTTTAGC TACCCAACCC
  781 GAGTCTATGA GCTTGACTTC AGATGTAGAA GGCATTAAAT CGTTTTGAAT ATTAATTAAA
  841 AAACGATGAA AATTAAATAT TTAAAAGCAA TCATACGCTG AAAATTTAGT GCTGTGGCTA
  901 ATCCTTCAAC ATGGAAATGC CATAAAAGTG ACTTTGACAA AAAAAAAAAG TATATACAGG
  961 TAGTAAACTC ATCTACTTCA TTTGACTTTG TTTACAGCAT GTGGGATGAG GAAATATTTA
 1021 TTTGCTAAAT CGTAGGTTTA ACATTTCAAT AAGTTAATTA CTATTGAAAA TCGACAGGAT
1081 GCGCATGCAA TGGAAAAAGA GGCCATTTTT GCCCTTTTGC G
//
Primer: f1ori_R3 RDB14497_A6L7b.seq
>06586_14497_A6L7_f1ori_R3_H09_24_ABI24.ab1
    1 GCAGTGGCTT CTTGGAGTAT GCGGTGTGAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
  241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
  301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
  361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
  421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
  481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
  541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
  601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
  661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
  721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
  781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG
  841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAAGAATG TTAAAACTAA TTTAACCTTT
  901 TTATAAAGGC TCATGGCGTT TAAAGAGGGA TAGTAAAAAT TTCTACAATT TTTTAATTTA
  961 ATGTCACAAT TTGTCTTGCG AATATAAATT CTATTAATTT TCGCTAACCT GCCTGTTTTA
 1021 GTACTATTAC TTTCAATATG CCTTATATGC AACTTTAAAT CATGAGGATT GCAATTATTT
1081 TGCATCCTTT CCTTGTACCT GAGCGCTTTT TATCAATTTT AAAG
//
Primer: LEU2pro_F RDB14497_A6L7c.seq
>06586_14497_A6L7_LEU2pro_F_G09_21_ABI24.ab1
    1 AACATGCAGT GAATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG
   61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATTTCAAGG
  121 ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG
  181 TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA
  241 TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT
  301 TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT TGTTAGGTGC
  361 TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT
  421 CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT
  481 TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG
  541 AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC
  601 TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT
  661 CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT
  721 GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AAATTCCCTA
  781 CATTGAAGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA GACCCCACCC
  841 ACCTAATGGT ATATAATCAC CAGCAACATG TTTGGTGACA TCATCTCGAT GAGGCCTCCG
  901 TTATCCAGTC CTGGGTTTGT GGCATCTGCG GTCCTTGGCC TCTTTGGCAG AACAAGAAAC
  961 ACCCGCCATT TGTTGTACCG ACATGCCACG TCTGCTCCGA TGGCAGATAG GTCACTATCG
1021 GCAATCTGTC CGCGCACAGT AGTGATTTGC CATG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

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