Please review the QC test results indicated by icon below as well as clone information before placing your order.

pFFH1

S. pombe expression vector with nmt1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14497_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw Sequence file: RDB14497_A6L7a.seq
>06586_14497_A6L7_nmt1Fw_F09_18_ABI24.ab1     1 TAGAAATTTT ATTAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA    61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT   121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA   181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC   241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG   301 GATCCGGATA TCATGGATTA TAAGGATGAT GACGATAAAC CCATGGACTA CAAGGACGAC   361 GATGACAAAC ATCACCACCA TCACCACTGA GATCTCGAGC CCGGGCGGCC GCTAACTAAC   421 TAAGGGTAAA AGGAATGTCT CCCTTGCCAG TACTGCTAGG GTTTTTCTTT CAAACTATGG   481 AAGCCCATTC AAGCTGCATA TTACGATTTT GTTTTTCGCT TTTAGAAAGT GGTTTAGATG   541 AGATAATAGA AAAATTCTTG ATCTCCGACA ACGAGTACTT TTATTTTTTT TGCTAATCAC   601 TTTACTCAAT ATTAGCTCGA AATCGTAGAA ACGTAGACGG GTGCGGGATA CCGAGTGGTG   661 TAGTTAAGAA TTTTTATAAA CCACGTGGCC CAAAAATATG AACCCAAAAC GTTTATACAT   721 GAGTATACTT TAAGAAGGCT ATACCCCTTC GTGTTAGATG TAGTTTTAGC TACCCAACCC   781 GAGTCTATGA GCTTGACTTC AGATGTAGAA GGCATTAAAT CGTTTTGAAT ATTAATTAAA   841 AAACGATGAA AATTAAATAT TTAAAAGCAA TCATACGCTG AAAATTTAGT GCTGTGGCTA   901 ATCCTTCAAC ATGGAAATGC CATAAAAGTG ACTTTGACAA AAAAAAAAAG TATATACAGG   961 TAGTAAACTC ATCTACTTCA TTTGACTTTG TTTACAGCAT GTGGGATGAG GAAATATTTA  1021 TTTGCTAAAT CGTAGGTTTA ACATTTCAAT AAGTTAATTA CTATTGAAAA TCGACAGGAT 1081 GCGCATGCAA TGGAAAAAGA GGCCATTTTT GCCCTTTTGC G //
Primer: f1ori_R3 Sequence file: RDB14497_A6L7b.seq
>06586_14497_A6L7_f1ori_R3_H09_24_ABI24.ab1     1 GCAGTGGCTT CTTGGAGTAT GCGGTGTGAT ACCGCACAGA TGCGTAAGGA GAAAATACCG    61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC   121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT   181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC   241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA   301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT   361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT   421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA   481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG   541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA   601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT   661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA   721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA   781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG   841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAAGAATG TTAAAACTAA TTTAACCTTT   901 TTATAAAGGC TCATGGCGTT TAAAGAGGGA TAGTAAAAAT TTCTACAATT TTTTAATTTA   961 ATGTCACAAT TTGTCTTGCG AATATAAATT CTATTAATTT TCGCTAACCT GCCTGTTTTA  1021 GTACTATTAC TTTCAATATG CCTTATATGC AACTTTAAAT CATGAGGATT GCAATTATTT 1081 TGCATCCTTT CCTTGTACCT GAGCGCTTTT TATCAATTTT AAAG //
Primer: LEU2pro_F Sequence file: RDB14497_A6L7c.seq
>06586_14497_A6L7_LEU2pro_F_G09_21_ABI24.ab1     1 AACATGCAGT GAATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG    61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATTTCAAGG   121 ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG   181 TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA   241 TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT   301 TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT TGTTAGGTGC   361 TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT   421 CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT   481 TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG   541 AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC   601 TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT   661 CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT   721 GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AAATTCCCTA   781 CATTGAAGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA GACCCCACCC   841 ACCTAATGGT ATATAATCAC CAGCAACATG TTTGGTGACA TCATCTCGAT GAGGCCTCCG   901 TTATCCAGTC CTGGGTTTGT GGCATCTGCG GTCCTTGGCC TCTTTGGCAG AACAAGAAAC   961 ACCCGCCATT TGTTGTACCG ACATGCCACG TCTGCTCCGA TGGCAGATAG GTCACTATCG 1021 GCAATCTGTC CGCGCACAGT AGTGATTTGC CATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.02.09

GNP_filter3_RDBDEP_html_220112.pl