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pHFG81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06585
Resource name pHFG81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06585 pHFG81 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06585z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14474_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14474_A6L7a.seq check
            >06585_14474_A6L7_nmt1Fw_E05_05_ABI08.ab1
                1 GGGAAATTTA TTTAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
              241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
              301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGGTAAAGG
              361 AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG
              421 GCACAAATTT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC
              481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT
              541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT
              601 TCAAGAGTGC CATGCCCGAA CGCTATGTAC AGGAAGAACT ATATTTTTCA AAGATGACGG
              661 GAACTACAGA CACGTGCTGA GTCAGTTTGA AGGTGGAATA CCCTTGTTAA TAGAATCGAG
              721 TTAAAAGGTA TTGATTTTAA GAAGATGGAA ACATTCTTGG ACACCAAATT GAATACACTA
              781 TAACTCCACC ACAATGTTTA CATCATGGCA ACAACAAAGA TGGATCAAAG TAACTTCAAA
              841 TTAGACCCAA CATTGAAGAT GGGAGCCTTC ACTAGCGAAC CATTATCAAC AAATACTCCA
              901 TGGCATGGCC CTGTTCTGTT AACAGGAACA ACAATATACT GTTCCACAGT CTTGCCTTCC
            961 GAAGATTCCA CGAAGAGGAG CCACTGCTCT GATTGAAACC CGTCTGGGAT A
            //
            Primer: nmt1ter_R
            Sequence file: RDB14474_A6L7b.seq check
            >06585_14474_A6L7_nmt1ter_R_C09_09_ABI24.ab1
                1 GACGTCGTAG TTACTCATGT ATAAACGTTT TGGGTTCATA TTTTTGGGCC ACGTGGTTTA
               61 TAAAAATTCT TAACTACACC ACTCGGTATC CCGCACCCGT CTACGTTTCT ACGATTTCGA
              121 GCTAATATTG AGTAAAGTGA TTAGCAAAAA AAATAAAAGT ACTCGTTGTC GGAGATCAAG
              181 AATTTTTCTA TTATCTCATC TAAACCACTT TCTAAAAGCG AAAAACAAAA TCGTAATATG
              241 CAGCTTGAAT GGGCTTCCAT AGTTTGAAAG AAAAACCCTA GCAGTACTGG CAAGGGAGAC
              301 ATTCCTTTTA CCCTTAGTTA GTTAGCGGCC GCCCGGGCTC GAGATCTCAT GATATCTTTG
              361 TATAGTTCAT CCATGCCATG TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG
              421 TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG
              481 TCTGGTAAAA GGACAGGGCC ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT
              541 TGAACGCTTC CATCTTCAAT GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT
              601 TGTTTGTCTG CCATGATGTA TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA
              661 AGAATGTTTC CATCTTCTTT AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA
              721 TCACCTTCAA ACTTGACTTC AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA
              781 GTTCTTTCCT GTACATAGCC TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG
              841 TGATCTGGGT ATCTTGAAAA GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC
              901 CACGGAACAG GTAGTTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTTCCGTAT
              961 GTTGCATCAC CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCCAT
             1021 CTAATTCACA AGATTGGGGA ACAACTCCCA GTGAAAAGCT CCTTCTCGTT TTACCATTGG
            1081 ATTTAATCGG TCAATTCAAT CCGTTATAAT TCCAATATTG GATGTGAAGT TG
            //
            Primer: f1ori_R3
            Sequence file: RDB14474_A6L7c.seq check
            >06585_14474_A6L7_f1ori_R3_E09_15_ABI24.ab1
                1 GCAATGGGGG CGTTGAGTAT GCGGTGTGAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
               61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
              121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
              181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
              241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
              301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
              361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
              421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
              481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
              541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
              601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
              661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
              721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
              781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG
              841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAAGAATG TTAAAACTAA TTTAACCTTT
              901 TTTATAAAGG CTCATGGCGT TTAAAGAGGA TAGTAAAAAA TTCTACAATT TTTTTAATTT
              961 AATGTCACAA ATTTGTCTTT GCGAAATATA ATTTCTATTT AATTTTTCGC TAACTGCTGT
             1021 TTTAAGTACT ATTACTTTCA ATATGCCCTT TATATGCAAC TTTAATCATG GAAGGAATGC
            1081 AATATTTTTG CATCCTTCCT TGGTACCTGA GCGCTTTTTA AATTCAATTT TAAGTTACT
            //
            Primer: LEU2pro_F
            Sequence file: RDB14474_A6L7d.seq check
            >06585_14474_A6L7_LEU2pro_F_D09_12_ABI24.ab1
                1 CACAGCAGTT GAATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG
               61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATTTCAAGG
              121 ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG
              181 TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA
              241 TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT
              301 TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT TGTTAGGTGC
              361 TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT
              421 CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT
              481 TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG
              541 AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC
              601 TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT
              661 CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT
              721 GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AATTCCCTAC
              781 ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA AGAACCCAAC
              841 CCACCTAAAT GGTATTATAA TCACCAGCAA CATGTTTGGT GACATCATCT CCGATGAAGC
              901 CCTCCGTTAT CCCAGGTTTC CTTGGGTTTG TTGCCATCTG CGTCCCTTGG GCCTCTTTTG
              961 CCAGACAGAA CACCGCATTT GGATTTGTAC GGACCATGCC ACGATCTGCT CCAGATTTGC
             1021 CAAAGATAGT CACCTATCGC ACTATCTGGT CTGCTGCATT GAATGTGATG TCATTGAACC
            1081 TTGGCCTGAG AGCTAAGGCC CATTGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl