Resource data sheet
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pHFG81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06585
Resource name pHFG81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06585 pHFG81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06585z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14474_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14474_A6L7a.seq
>06585_14474_A6L7_nmt1Fw_E05_05_ABI08.ab1
    1 GGGAAATTTA TTTAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
  241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
  301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGGTAAAGG
  361 AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA TTAGATGGTG ATGTTAATGG
  421 GCACAAATTT TTCTGTCAGT GGAGAGGGTG AAGGTGATGC AACATACGGA AAACTTACCC
  481 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTT
  541 TCTGTTATGG TGTTCAATGC TTTTCAAGAT ACCCAGATCA CATGAAACAG CATGACTTTT
  601 TCAAGAGTGC CATGCCCGAA CGCTATGTAC AGGAAGAACT ATATTTTTCA AAGATGACGG
  661 GAACTACAGA CACGTGCTGA GTCAGTTTGA AGGTGGAATA CCCTTGTTAA TAGAATCGAG
  721 TTAAAAGGTA TTGATTTTAA GAAGATGGAA ACATTCTTGG ACACCAAATT GAATACACTA
  781 TAACTCCACC ACAATGTTTA CATCATGGCA ACAACAAAGA TGGATCAAAG TAACTTCAAA
  841 TTAGACCCAA CATTGAAGAT GGGAGCCTTC ACTAGCGAAC CATTATCAAC AAATACTCCA
  901 TGGCATGGCC CTGTTCTGTT AACAGGAACA ACAATATACT GTTCCACAGT CTTGCCTTCC
961 GAAGATTCCA CGAAGAGGAG CCACTGCTCT GATTGAAACC CGTCTGGGAT A
//
Primer: nmt1ter_R RDB14474_A6L7b.seq
>06585_14474_A6L7_nmt1ter_R_C09_09_ABI24.ab1
    1 GACGTCGTAG TTACTCATGT ATAAACGTTT TGGGTTCATA TTTTTGGGCC ACGTGGTTTA
   61 TAAAAATTCT TAACTACACC ACTCGGTATC CCGCACCCGT CTACGTTTCT ACGATTTCGA
  121 GCTAATATTG AGTAAAGTGA TTAGCAAAAA AAATAAAAGT ACTCGTTGTC GGAGATCAAG
  181 AATTTTTCTA TTATCTCATC TAAACCACTT TCTAAAAGCG AAAAACAAAA TCGTAATATG
  241 CAGCTTGAAT GGGCTTCCAT AGTTTGAAAG AAAAACCCTA GCAGTACTGG CAAGGGAGAC
  301 ATTCCTTTTA CCCTTAGTTA GTTAGCGGCC GCCCGGGCTC GAGATCTCAT GATATCTTTG
  361 TATAGTTCAT CCATGCCATG TGTAATCCCA GCAGCTGTTA CAAACTCAAG AAGGACCATG
  421 TGGTCTCTCT TTTCGTTGGG ATCTTTCGAA AGGGCAGATT GTGTGGACAG GTAATGGTTG
  481 TCTGGTAAAA GGACAGGGCC ATCGCCAATT GGAGTATTTT GTTGATAATG GTCTGCTAGT
  541 TGAACGCTTC CATCTTCAAT GTTGTGTCTA ATTTTGAAGT TAACTTTGAT TCCATTCTTT
  601 TGTTTGTCTG CCATGATGTA TACATTGTGT GAGTTATAGT TGTATTCCAA TTTGTGTCCA
  661 AGAATGTTTC CATCTTCTTT AAAATCAATA CCTTTTAACT CGATTCTATT AACAAGGGTA
  721 TCACCTTCAA ACTTGACTTC AGCACGTGTC TTGTAGTTCC CGTCATCTTT GAAAAATATA
  781 GTTCTTTCCT GTACATAGCC TTCGGGCATG GCACTCTTGA AAAAGTCATG CTGTTTCATG
  841 TGATCTGGGT ATCTTGAAAA GCATTGAACA CCATAACAGA AAGTAGTGAC AAGTGTTGGC
  901 CACGGAACAG GTAGTTTTTC CAGTAGTGCA AATAAATTTA AAGGGTAAGT TTTTCCGTAT
  961 GTTGCATCAC CCTTCACCCT CTCCACTGAC AGAAAATTTG TGCCCATTAA CATCACCCAT
 1021 CTAATTCACA AGATTGGGGA ACAACTCCCA GTGAAAAGCT CCTTCTCGTT TTACCATTGG
1081 ATTTAATCGG TCAATTCAAT CCGTTATAAT TCCAATATTG GATGTGAAGT TG
//
Primer: f1ori_R3 RDB14474_A6L7c.seq
>06585_14474_A6L7_f1ori_R3_E09_15_ABI24.ab1
    1 GCAATGGGGG CGTTGAGTAT GCGGTGTGAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
  241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
  301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
  361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
  421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
  481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
  541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
  601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
  661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
  721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
  781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG
  841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAAGAATG TTAAAACTAA TTTAACCTTT
  901 TTTATAAAGG CTCATGGCGT TTAAAGAGGA TAGTAAAAAA TTCTACAATT TTTTTAATTT
  961 AATGTCACAA ATTTGTCTTT GCGAAATATA ATTTCTATTT AATTTTTCGC TAACTGCTGT
 1021 TTTAAGTACT ATTACTTTCA ATATGCCCTT TATATGCAAC TTTAATCATG GAAGGAATGC
1081 AATATTTTTG CATCCTTCCT TGGTACCTGA GCGCTTTTTA AATTCAATTT TAAGTTACT
//
Primer: LEU2pro_F RDB14474_A6L7d.seq
>06585_14474_A6L7_LEU2pro_F_D09_12_ABI24.ab1
    1 CACAGCAGTT GAATATTATT TAAGGACCTA TTGTTTTTTC CAATAGGTGG TTAGCAATCG
   61 TCTTACTTTC TAACTTTTCT TACCTTTTAC ATTTCAGCAA TATATATATA TATTTCAAGG
  121 ATATACCATT CTAATGTCTG CCCCTAAGAA GATCGTCGTT TTGCCAGGTG ACCACGTTGG
  181 TCAAGAAATC ACAGCCGAAG CCATTAAGGT TCTTAAAGCT ATTTCTGATG TTCGTTCCAA
  241 TGTCAAGTTC GATTTCGAAA ATCATTTAAT TGGTGGTGCT GCTATCGATG CTACAGGTGT
  301 TCCACTTCCA GATGAGGCGC TGGAAGCCTC CAAGAAGGCT GATGCCGTTT TGTTAGGTGC
  361 TGTGGGTGGT CCTAAATGGG GTACCGGTAG TGTTAGACCT GAACAAGGTT TACTAAAAAT
  421 CCGTAAAGAA CTTCAATTGT ACGCCAACTT AAGACCATGT AACTTTGCAT CCGACTCTCT
  481 TTTAGACTTA TCTCCAATCA AGCCACAATT TGCTAAAGGT ACTGACTTCG TTGTTGTCAG
  541 AGAATTAGTG GGAGGTATTT ACTTTGGTAA GAGAAAGGAA GACGATGGTG ATGGTGTCGC
  601 TTGGGATAGT GAACAATACA CCGTTCCAGA AGTGCAAAGA ATCACAAGAA TGGCCGCTTT
  661 CATGGCCCTA CAACATGAGC CACCATTGCC TATTTGGTCC TTGGATAAAG CTAATGTTTT
  721 GGCCTCTTCA AGATTATGGA GAAAAACTGT GGAGGAAACC ATCAAGAACG AATTCCCTAC
  781 ATTGAAGGTT CAACATCAAT TGATTGATTC TGCCGCCATG ATCCTAGTTA AGAACCCAAC
  841 CCACCTAAAT GGTATTATAA TCACCAGCAA CATGTTTGGT GACATCATCT CCGATGAAGC
  901 CCTCCGTTAT CCCAGGTTTC CTTGGGTTTG TTGCCATCTG CGTCCCTTGG GCCTCTTTTG
  961 CCAGACAGAA CACCGCATTT GGATTTGTAC GGACCATGCC ACGATCTGCT CCAGATTTGC
 1021 CAAAGATAGT CACCTATCGC ACTATCTGGT CTGCTGCATT GAATGTGATG TCATTGAACC
1081 TTGGCCTGAG AGCTAAGGCC CATTGA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl