Resource data sheet
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pHFG41

S. pombe expression vector with nmt1 promoter

Catalog number RDB06584
Resource name pHFG41
Clone info. S. pombe 5' ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter.
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06584 pHFG41 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06584z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14473_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14473_A6L7a.seq
>06584_14473_A6L7_nmt1Fw_C05_03_ABI08.ab1
    1 TGGAAATTAA TTAAATCAGG GAAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
   61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
  121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
  181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
  241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
  301 CCTATGCATC ACCACCATCA TCATATGGAT TATAAGGATG ATGACGATAA ACCCATGGGT
  361 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT
  421 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT
  481 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CGTGGCCAAC ACTTGTCACT
  541 ACTTTCTGTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG ATCACATGAA ACAGCATGAC
  601 TTTTTCAAGA GTGCCATGCC CGAAGGCTAT GTACAGGAAA GAACTATATT TTTCAAAGAT
  661 GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA
  721 ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA
  781 TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA
  841 GTTAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT
  901 CAACAAAATA CTCCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC CATTACCTGT
  961 CCACACAATC TGCCCTTTCG AAAGATCCCA AACGAAAAGA GAGACCACAT GGGTCCCTTT
 1021 CTTGAGTTTG TAACAGCCTG CCTGGGGAAT ACACATGGGC ATTGATGAAC TATACGAAGG
1081 ATATCAATTG AGAATCTCGA AGCCCCAAGG ACCGGATCT
//
Primer: nmt1ter_R RDB14473_A6L7b.seq
>06584_14473_A6L7_nmt1ter_R_G08_20_ABI24.ab1
    1 GTCTGTAGTT ACTCATGTAT AACGTTTTGG GTTCATATTT TTGGGCCACG TGGTTTATAA
   61 AAATTCTTAA CTACACCACT CGGTATCCCG CACCCGTCTA CGTTTCTACG ATTTCGAGCT
  121 AATATTGAGT AAAGTGATTA GCAAAAAAAA TAAAAGTACT CGTTGTCGGA GATCAAGAAT
  181 TTTTCTATTA TCTCATCTAA ACCACTTTCT AAAAGCGAAA AACAAAATCG TAATATGCAG
  241 CTTGAATGGG CTTCCATAGT TTGAAAGAAA AACCCTAGCA GTACTGGCAA GGGAGACATT
  301 CCTTTTACCC TTAGTTAGTT AGCGGCCGCC CGGGCTCGAG ATCTCATGAT ATCTTTGTAT
  361 AGTTCATCCA TGCCATGTGT AATCCCAGCA GCTGTTACAA ACTCAAGAAG GACCATGTGG
  421 TCTCTCTTTT CGTTGGGATC TTTCGAAAGG GCAGATTGTG TGGACAGGTA ATGGTTGTCT
  481 GGTAAAAGGA CAGGGCCATC GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA
  541 ACGCTTCCAT CTTCAATGTT GTGTCTAATT TTGAAGTTAA CTTTGATTCC ATTCTTTTGT
  601 TTGTCTGCCA TGATGTATAC ATTGTGTGAG TTATAGTTGT ATTCCAATTT GTGTCCAAGA
  661 ATGTTTCCAT CTTCTTTAAA ATCAATACCT TTTAACTCGA TTCTATTAAC AAGGGTATCA
  721 CCTTCAAACT TGACTTCAGC ACGTGTCTTG TAGTTCCCGT CATCTTTGAA AAATATAGTT
  781 CTTTCCTGTA CATAGCCTTC GGGCATGGCA CTCTTGAAAA AGTCATGCTG TTTCATGTGA
  841 TCTGGGTATC TTGAAAAGCA TTGAACACCA TAACAGAAAG TAGTGACAAG TGTTGGGCCA
  901 CGGAACAGTA GTTTTCCAGT AGTGCAAATA AATTTTAAGG TAAGTTTTCC CGTATGTTGC
  961 ATCACCTTCA CCCTCTCCAC TGACAGAAAA ATTTGTGCCA TACATCACAT CTAATCACCA
 1021 AGAATGGGAC CACTCAGTGA AAAGTCTCTC GTTACCAATG TCTATCGTCA TCATCGTTAT
1081 ATTCGAATTT GAATGGATGG GCTAGT
//
Primer: f1ori_R3 RDB14473_A6L7c.seq
>06584_14473_A6L7_f1ori_R3_A09_03_ABI24.ab1
    1 TGGCGTTGGT TGCTTTCCGT ATGCGGTGTG AATACCGCAC AGATGGCGTA AAGGAGAAAA
   61 TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG
  121 CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT
  181 TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTCCAA
  241 CCTTCCAATT CATTAAATCG AGTCCTCCAT CAGATGATTT ACAAGGTTTT GCATAGAATC
  301 CCTAAGTAAG AGAAGTTGTT AGTGATTTCT TCAAGTTTAC TAAAATTTAC ATACAAATGG
  361 ATGATCTCTT CTCCATTGTT TCCTTTCTTC TTGCAAACGC GTTTTACAAA GAGATGACAT
  421 GCTATTTTCT GATTATTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG GGAGTCCGAA
  481 ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA AAAGATAAAA
  541 AGTAGGGGCA AGCGAGACAG AATGGGATAC AAGGGCATCG TCTATAATTA TAGCTAAAAA
  601 TTGTATTTTA ATTTGTATTT TTTGTAATTT TATTTCTCAT TGTTTTACTT AAAATGAATG
  661 CGAATTAGAG AAAACTTCAA CGAAATGTCA AAATAAGCTC AGCAAAATAT ACAATTTTAG
  721 GGAAAGCGAT CAGCAACTTA TTCTCCGTTG TAGATTCTTT TTCTTCATAA ATTCCAACAA
  781 TGTAACTGCC AATTGATTTT TCTCTAGATG CTAATAAATT GAAACTAATG AATATAATAA
  841 CACAGGTTTT GATAAATTGT AAACTCTTAC TCAAAAAAAG AATGTTAAAA CTAATTTAAC
  901 CCTTTTTATA AAGGCTCATG GCCGTTAAAG AGGATAGTAA AAAATTCCTA CAATTTTTTA
  961 ATTTAATGTC ACAATTTGTC TTGCGAAATA TAATTCTATT AAATTTTCCG CTAACCTGCT
 1021 GTTTTAGGTA CCTATTTACT TTCCATATGC CCTATATGCA CTTTATTCAT GAGAATGCAA
1081 TATTTTGCAT CTTTCTTGGT ACTGAGCCGC CTTTTTTTAA TTCCAATTTT AGGTTAC
//
Primer: LEU2pro_F RDB14473_A6L7d.seq
>06584_14473_A6L7_LEU2pro_F_H08_23_ABI24.ab1
    1 CATATTGAGT TGAATATTAT TTAAGGACCT ATTGTTTTTT CCAATAGGTG GTTAGCAATC
   61 GTCTTACTTT CTAACTTTTC TTACCTTTTA CATTTCAGCA ATATATATAT ATATTTCAAG
  121 GATATACCAT TCTAATGTCT GCCCCTAAGA AGATCGTCGT TTTGCCAGGT GACCACGTTG
  181 GTCAAGAAAT CACAGCCGAA GCCATTAAGG TTCTTAAAGC TATTTCTGAT GTTCGTTCCA
  241 ATGTCAAGTT CGATTTCGAA AATCATTTAA TTGGTGGTGC TGCTATCGAT GCTACAGGTG
  301 TTCCACTTCC AGATGAGGCG CTGGAAGCCT CCAAGAAGGC TGATGCCGTT TTGTTAGGTG
  361 CTGTGGGTGG TCCTAAATGG GGTACCGGTA GTGTTAGACC TGAACAAGGT TTACTAAAAA
  421 TCCGTAAAGA ACTTCAATTG TACGCCAACT TAAGACCATG TAACTTTGCA TCCGACTCTC
  481 TTTTAGACTT ATCTCCAATC AAGCCACAAT TTGCTAAAGG TACTGACTTC GTTGTTGTCA
  541 GAGAATTAGT GGGAGGTATT TACTTTGGTA AGAGAAAGGA AGACGATGGT GATGGTGTCG
  601 CTTGGGATAG TGAACAATAC ACCGTTCCAG AAGTGCAAAG AATCACAAGA ATGGCCGCTT
  661 TCATGGCCCT ACAACATGAG CCACCATTGC CTATTTGGTC CTTGGATAAA GCTAATGTTT
  721 TGGCCTCTTC AAGATTATGG AGAAAAACTG TGGAGGAAAC CATCAAGAAC GAATTCCCTA
  781 CATTGAAGGT TCAACATCAA TTGATTGATT CTGCCGCCAT GATCCTAGTT AAGAACCCAA
  841 CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGACATCATC TCCGATGAAG
  901 CCTCCGTTAT CCCAGGTTCC TTGGGTTTGT TGCCATCTGC GTCCTTTGGC CTCTTTGCCA
  961 GACAAGAACA CCGCATTTGG GTTTGTACGA ACCATGCCAC GGTCTGCTCC AGATTTGCCA
 1021 AGGATAGTCC ACCCTATCGC ACTATCTGTC TGCTGCAATG AATGTGATGT CATTGAACTT
1081 GCTGAGAAGT AAAGGCCATT GA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl