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pHFG41

S. pombe expression vector with nmt1 promoter

Catalog number RDB06584
Resource name pHFG41
Clone info. S. pombe 5' ter end His6-FLAG-GFP(S65C)-tagged vector with nmt1 (medium strength) promoter.
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Schedule A (GFP-A) [Link]
            Remarks
            Please use Schedule A (GFP-A) additionally.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            Schedule A(GFP-A, 英語版のみ) [Link]
            備考
            Schedule A(GFP-A, 英語版のみ)が追加で必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06584 pHFG41 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06584z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14473_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14473_A6L7a.seq check
            >06584_14473_A6L7_nmt1Fw_C05_03_ABI08.ab1
                1 TGGAAATTAA TTAAATCAGG GAAAAACGTA ACTCTCGGCT ACTGGATGGT TCAGTCACCC
               61 AACGATTACT GGGGAGAGAA AACAGGGCAA AAGCAAAGCT TAAAGGAATC CGATTGTCAT
              121 TCGGCAATGT GCAGCGAAAC TAAAAACCGG ATAATGGACC TGTTAATCGA AACATTGAAG
              181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
              241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
              301 CCTATGCATC ACCACCATCA TCATATGGAT TATAAGGATG ATGACGATAA ACCCATGGGT
              361 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT
              421 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT
              481 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CGTGGCCAAC ACTTGTCACT
              541 ACTTTCTGTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG ATCACATGAA ACAGCATGAC
              601 TTTTTCAAGA GTGCCATGCC CGAAGGCTAT GTACAGGAAA GAACTATATT TTTCAAAGAT
              661 GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA
              721 ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA
              781 TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA
              841 GTTAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT
              901 CAACAAAATA CTCCCAATTG GCGATGGCCC TGTCCTTTTA CCAGACAACC CATTACCTGT
              961 CCACACAATC TGCCCTTTCG AAAGATCCCA AACGAAAAGA GAGACCACAT GGGTCCCTTT
             1021 CTTGAGTTTG TAACAGCCTG CCTGGGGAAT ACACATGGGC ATTGATGAAC TATACGAAGG
            1081 ATATCAATTG AGAATCTCGA AGCCCCAAGG ACCGGATCT
            //
            Primer: nmt1ter_R
            Sequence file: RDB14473_A6L7b.seq check
            >06584_14473_A6L7_nmt1ter_R_G08_20_ABI24.ab1
                1 GTCTGTAGTT ACTCATGTAT AACGTTTTGG GTTCATATTT TTGGGCCACG TGGTTTATAA
               61 AAATTCTTAA CTACACCACT CGGTATCCCG CACCCGTCTA CGTTTCTACG ATTTCGAGCT
              121 AATATTGAGT AAAGTGATTA GCAAAAAAAA TAAAAGTACT CGTTGTCGGA GATCAAGAAT
              181 TTTTCTATTA TCTCATCTAA ACCACTTTCT AAAAGCGAAA AACAAAATCG TAATATGCAG
              241 CTTGAATGGG CTTCCATAGT TTGAAAGAAA AACCCTAGCA GTACTGGCAA GGGAGACATT
              301 CCTTTTACCC TTAGTTAGTT AGCGGCCGCC CGGGCTCGAG ATCTCATGAT ATCTTTGTAT
              361 AGTTCATCCA TGCCATGTGT AATCCCAGCA GCTGTTACAA ACTCAAGAAG GACCATGTGG
              421 TCTCTCTTTT CGTTGGGATC TTTCGAAAGG GCAGATTGTG TGGACAGGTA ATGGTTGTCT
              481 GGTAAAAGGA CAGGGCCATC GCCAATTGGA GTATTTTGTT GATAATGGTC TGCTAGTTGA
              541 ACGCTTCCAT CTTCAATGTT GTGTCTAATT TTGAAGTTAA CTTTGATTCC ATTCTTTTGT
              601 TTGTCTGCCA TGATGTATAC ATTGTGTGAG TTATAGTTGT ATTCCAATTT GTGTCCAAGA
              661 ATGTTTCCAT CTTCTTTAAA ATCAATACCT TTTAACTCGA TTCTATTAAC AAGGGTATCA
              721 CCTTCAAACT TGACTTCAGC ACGTGTCTTG TAGTTCCCGT CATCTTTGAA AAATATAGTT
              781 CTTTCCTGTA CATAGCCTTC GGGCATGGCA CTCTTGAAAA AGTCATGCTG TTTCATGTGA
              841 TCTGGGTATC TTGAAAAGCA TTGAACACCA TAACAGAAAG TAGTGACAAG TGTTGGGCCA
              901 CGGAACAGTA GTTTTCCAGT AGTGCAAATA AATTTTAAGG TAAGTTTTCC CGTATGTTGC
              961 ATCACCTTCA CCCTCTCCAC TGACAGAAAA ATTTGTGCCA TACATCACAT CTAATCACCA
             1021 AGAATGGGAC CACTCAGTGA AAAGTCTCTC GTTACCAATG TCTATCGTCA TCATCGTTAT
            1081 ATTCGAATTT GAATGGATGG GCTAGT
            //
            Primer: f1ori_R3
            Sequence file: RDB14473_A6L7c.seq check
            >06584_14473_A6L7_f1ori_R3_A09_03_ABI24.ab1
                1 TGGCGTTGGT TGCTTTCCGT ATGCGGTGTG AATACCGCAC AGATGGCGTA AAGGAGAAAA
               61 TACCGCATCA GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG
              121 CGGGCCTCTT CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT
              181 TGGGTAACGC CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTCCAA
              241 CCTTCCAATT CATTAAATCG AGTCCTCCAT CAGATGATTT ACAAGGTTTT GCATAGAATC
              301 CCTAAGTAAG AGAAGTTGTT AGTGATTTCT TCAAGTTTAC TAAAATTTAC ATACAAATGG
              361 ATGATCTCTT CTCCATTGTT TCCTTTCTTC TTGCAAACGC GTTTTACAAA GAGATGACAT
              421 GCTATTTTCT GATTATTTTT TTCTATAGTT TTCTATTTTG TACGTCCTTG GGAGTCCGAA
              481 ATGTAAAATC GGGGTATGGT TAGTAGCGGC AGCGTTCATT ATGGTATATA AAAGATAAAA
              541 AGTAGGGGCA AGCGAGACAG AATGGGATAC AAGGGCATCG TCTATAATTA TAGCTAAAAA
              601 TTGTATTTTA ATTTGTATTT TTTGTAATTT TATTTCTCAT TGTTTTACTT AAAATGAATG
              661 CGAATTAGAG AAAACTTCAA CGAAATGTCA AAATAAGCTC AGCAAAATAT ACAATTTTAG
              721 GGAAAGCGAT CAGCAACTTA TTCTCCGTTG TAGATTCTTT TTCTTCATAA ATTCCAACAA
              781 TGTAACTGCC AATTGATTTT TCTCTAGATG CTAATAAATT GAAACTAATG AATATAATAA
              841 CACAGGTTTT GATAAATTGT AAACTCTTAC TCAAAAAAAG AATGTTAAAA CTAATTTAAC
              901 CCTTTTTATA AAGGCTCATG GCCGTTAAAG AGGATAGTAA AAAATTCCTA CAATTTTTTA
              961 ATTTAATGTC ACAATTTGTC TTGCGAAATA TAATTCTATT AAATTTTCCG CTAACCTGCT
             1021 GTTTTAGGTA CCTATTTACT TTCCATATGC CCTATATGCA CTTTATTCAT GAGAATGCAA
            1081 TATTTTGCAT CTTTCTTGGT ACTGAGCCGC CTTTTTTTAA TTCCAATTTT AGGTTAC
            //
            Primer: LEU2pro_F
            Sequence file: RDB14473_A6L7d.seq check
            >06584_14473_A6L7_LEU2pro_F_H08_23_ABI24.ab1
                1 CATATTGAGT TGAATATTAT TTAAGGACCT ATTGTTTTTT CCAATAGGTG GTTAGCAATC
               61 GTCTTACTTT CTAACTTTTC TTACCTTTTA CATTTCAGCA ATATATATAT ATATTTCAAG
              121 GATATACCAT TCTAATGTCT GCCCCTAAGA AGATCGTCGT TTTGCCAGGT GACCACGTTG
              181 GTCAAGAAAT CACAGCCGAA GCCATTAAGG TTCTTAAAGC TATTTCTGAT GTTCGTTCCA
              241 ATGTCAAGTT CGATTTCGAA AATCATTTAA TTGGTGGTGC TGCTATCGAT GCTACAGGTG
              301 TTCCACTTCC AGATGAGGCG CTGGAAGCCT CCAAGAAGGC TGATGCCGTT TTGTTAGGTG
              361 CTGTGGGTGG TCCTAAATGG GGTACCGGTA GTGTTAGACC TGAACAAGGT TTACTAAAAA
              421 TCCGTAAAGA ACTTCAATTG TACGCCAACT TAAGACCATG TAACTTTGCA TCCGACTCTC
              481 TTTTAGACTT ATCTCCAATC AAGCCACAAT TTGCTAAAGG TACTGACTTC GTTGTTGTCA
              541 GAGAATTAGT GGGAGGTATT TACTTTGGTA AGAGAAAGGA AGACGATGGT GATGGTGTCG
              601 CTTGGGATAG TGAACAATAC ACCGTTCCAG AAGTGCAAAG AATCACAAGA ATGGCCGCTT
              661 TCATGGCCCT ACAACATGAG CCACCATTGC CTATTTGGTC CTTGGATAAA GCTAATGTTT
              721 TGGCCTCTTC AAGATTATGG AGAAAAACTG TGGAGGAAAC CATCAAGAAC GAATTCCCTA
              781 CATTGAAGGT TCAACATCAA TTGATTGATT CTGCCGCCAT GATCCTAGTT AAGAACCCAA
              841 CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGACATCATC TCCGATGAAG
              901 CCTCCGTTAT CCCAGGTTCC TTGGGTTTGT TGCCATCTGC GTCCTTTGGC CTCTTTGCCA
              961 GACAAGAACA CCGCATTTGG GTTTGTACGA ACCATGCCAC GGTCTGCTCC AGATTTGCCA
             1021 AGGATAGTCC ACCCTATCGC ACTATCTGTC TGCTGCAATG AATGTGATGT CATTGAACTT
            1081 GCTGAGAAGT AAAGGCCATT GA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl