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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHFF81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06582
Resource name pHFF81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06582 pHFF81 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06582z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14496_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14496_A6L7a.seq check
            >06582_14496_A6L7_nmt1Fw_G07_19_ABI24.ab1
                1 AGCATTTTTA TTAATCAGGA AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
               61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
              121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
              181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
              241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
              301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA
              361 GGACGACGAT GACAAAGATA TCATGAGATC TCGAGCCCGG GCGGCCGCTA ACTAACTAAG
              421 GGTAAAAGGA ATGTCTCCCT TGCCAGTACT GCTAGGGTTT TTCTTTCAAA CTATGGAAGC
              481 CCATTCAAGC TGCATATTAC GATTTTGTTT TTCGCTTTTA GAAAGTGGTT TAGATGAGAT
              541 AATAGAAAAA TTCTTGATCT CCGACAACGA GTACTTTTAT TTTTTTTGCT AATCACTTTA
              601 CTCAATATTA GCTCGAAATC GTAGAAACGT AGACGGGTGC GGGATACCGA GTGGTGTCCT
              661 AAGAATGTTT TATAAACCAC GTGGCCCACA AATATGAACC CAAAACGTTT ATACATGAGT
              721 ATACTTTAAG AAGGCTATAC CCCTTCGTGT TAGATGTAGT TTTAGCTACC CAACCCGAGT
              781 CTATGAGCTT GACTTCAGAT GTAGAAAGCA TTAAATCGTT TTGAATATTA ATTAAAAAAC
              841 GATGAAAATT AAATATTTAA AAGCAATCAT ACGCTGAAAA TTTAGTGCTG TGGCTAATCC
              901 TTCAACATGG AAAATGCCAT AAAAGTGACT TTGACAAAAA AAAAAAGTAT ATACAGGTAG
              961 TAACTTCATC TACTCATGAC TTTTGTTTAC AGCATGTGGA AGAAGATATT TATTGCTAAT
             1021 CGTTAGGTTT ACCATCCATT AGTTATAACT ATTGATCGGA CAAGATTTGA CGCATGATGA
            1081 AAACAGCATT AGCTTTCGGA GAAAATTTAG GTC
            //
            Primer: f1ori_R3
            Sequence file: RDB14496_A6L7b.seq check
            >06582_14496_A6L7_f1ori_R3_A08_02_ABI24.ab1
                1 GCAGGGCGGC ATTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
               61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
              121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
              181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
              241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
              301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
              361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
              421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
              481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
              541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
              601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
              661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
              721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
              781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG
              841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT
              901 TTATAAAGGC TCATGGCGTT AAAGAGGATA GTAAAAAATT CTACAATTTT TTTAAATTTA
              961 ATGTCACAAT TTGTCTTTGC GAAATATAAT TTCTATTTAA TTTTCGCTAC TGCTGTTTTA
             1021 GTACTATTAA CTTTCAATAT GCCCTTATAT GCAACTTTAA TCATGCAGAT GCAATATTTT
            1081 GCATCCTTCT GGTACTGAAG CGCTTTTTTT AATCATTTTA G
            //
            Primer: LEU2pro_F
            Sequence file: RDB14496_A6L7c.seq check
            >06582_14496_A6L7_LEU2pro_F_H07_22_ABI24.ab1
                1 AATTTTGCAG TTGAAATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
               61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
              121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
              181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
              241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
              301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
              361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
              421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
              481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
              541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
              601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
              661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
              721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
              781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
              841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
              901 AGCCTCCGTT ATCCCAGGTT CCTTGGGTTT GTTGCCATCT GCGTCCTTGG GCCTCTTTGC
              961 CAGACAAGAC ACCGCATTTT GGGTTTGTAC GAACCAATGC ACGATTCTGC CTCCAGATTT
             1021 GCCAAAGAAT ACGTCCACCC CTATCGCAAC TATCCTGTCT GCTGCCATTG AATGTGGAAA
            1081 TGTCATTGAA CTGGCTGAAG AGGTAGGGCC ATTTGGAAG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl