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pHFF81

S. pombe expression vector with nmt1 promoter

Catalog number RDB06582
Resource name pHFF81
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor Yoshida, Minoru | Matsuyama, Akihisa |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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RDB06582 pHFF81 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB06582z.seq from Depositor

Featured content

Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14496_A6L7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw RDB14496_A6L7a.seq
>06582_14496_A6L7_nmt1Fw_G07_19_ABI24.ab1
    1 AGCATTTTTA TTAATCAGGA AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
  241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
  301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA
  361 GGACGACGAT GACAAAGATA TCATGAGATC TCGAGCCCGG GCGGCCGCTA ACTAACTAAG
  421 GGTAAAAGGA ATGTCTCCCT TGCCAGTACT GCTAGGGTTT TTCTTTCAAA CTATGGAAGC
  481 CCATTCAAGC TGCATATTAC GATTTTGTTT TTCGCTTTTA GAAAGTGGTT TAGATGAGAT
  541 AATAGAAAAA TTCTTGATCT CCGACAACGA GTACTTTTAT TTTTTTTGCT AATCACTTTA
  601 CTCAATATTA GCTCGAAATC GTAGAAACGT AGACGGGTGC GGGATACCGA GTGGTGTCCT
  661 AAGAATGTTT TATAAACCAC GTGGCCCACA AATATGAACC CAAAACGTTT ATACATGAGT
  721 ATACTTTAAG AAGGCTATAC CCCTTCGTGT TAGATGTAGT TTTAGCTACC CAACCCGAGT
  781 CTATGAGCTT GACTTCAGAT GTAGAAAGCA TTAAATCGTT TTGAATATTA ATTAAAAAAC
  841 GATGAAAATT AAATATTTAA AAGCAATCAT ACGCTGAAAA TTTAGTGCTG TGGCTAATCC
  901 TTCAACATGG AAAATGCCAT AAAAGTGACT TTGACAAAAA AAAAAAGTAT ATACAGGTAG
  961 TAACTTCATC TACTCATGAC TTTTGTTTAC AGCATGTGGA AGAAGATATT TATTGCTAAT
 1021 CGTTAGGTTT ACCATCCATT AGTTATAACT ATTGATCGGA CAAGATTTGA CGCATGATGA
1081 AAACAGCATT AGCTTTCGGA GAAAATTTAG GTC
//
Primer: f1ori_R3 RDB14496_A6L7b.seq
>06582_14496_A6L7_f1ori_R3_A08_02_ABI24.ab1
    1 GCAGGGCGGC ATTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
  241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
  301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
  361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
  421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
  481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
  541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
  601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
  661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
  721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
  781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG
  841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT
  901 TTATAAAGGC TCATGGCGTT AAAGAGGATA GTAAAAAATT CTACAATTTT TTTAAATTTA
  961 ATGTCACAAT TTGTCTTTGC GAAATATAAT TTCTATTTAA TTTTCGCTAC TGCTGTTTTA
 1021 GTACTATTAA CTTTCAATAT GCCCTTATAT GCAACTTTAA TCATGCAGAT GCAATATTTT
1081 GCATCCTTCT GGTACTGAAG CGCTTTTTTT AATCATTTTA G
//
Primer: LEU2pro_F RDB14496_A6L7c.seq
>06582_14496_A6L7_LEU2pro_F_H07_22_ABI24.ab1
    1 AATTTTGCAG TTGAAATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
   61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
  901 AGCCTCCGTT ATCCCAGGTT CCTTGGGTTT GTTGCCATCT GCGTCCTTGG GCCTCTTTGC
  961 CAGACAAGAC ACCGCATTTT GGGTTTGTAC GAACCAATGC ACGATTCTGC CTCCAGATTT
 1021 GCCAAAGAAT ACGTCCACCC CTATCGCAAC TATCCTGTCT GCTGCCATTG AATGTGGAAA
1081 TGTCATTGAA CTGGCTGAAG AGGTAGGGCC ATTTGGAAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl