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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHFF81 (#RDB06582)

S. pombe expression vector with nmt1 promoter

Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06582z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06582 pHFF81 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHFF81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06582).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14496_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14496_A6L7a.seq check
>06582_14496_A6L7_nmt1Fw_G07_19_ABI24.ab1
    1 AGCATTTTTA TTAATCAGGA AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT
  241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT
  301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA
  361 GGACGACGAT GACAAAGATA TCATGAGATC TCGAGCCCGG GCGGCCGCTA ACTAACTAAG
  421 GGTAAAAGGA ATGTCTCCCT TGCCAGTACT GCTAGGGTTT TTCTTTCAAA CTATGGAAGC
  481 CCATTCAAGC TGCATATTAC GATTTTGTTT TTCGCTTTTA GAAAGTGGTT TAGATGAGAT
  541 AATAGAAAAA TTCTTGATCT CCGACAACGA GTACTTTTAT TTTTTTTGCT AATCACTTTA
  601 CTCAATATTA GCTCGAAATC GTAGAAACGT AGACGGGTGC GGGATACCGA GTGGTGTCCT
  661 AAGAATGTTT TATAAACCAC GTGGCCCACA AATATGAACC CAAAACGTTT ATACATGAGT
  721 ATACTTTAAG AAGGCTATAC CCCTTCGTGT TAGATGTAGT TTTAGCTACC CAACCCGAGT
  781 CTATGAGCTT GACTTCAGAT GTAGAAAGCA TTAAATCGTT TTGAATATTA ATTAAAAAAC
  841 GATGAAAATT AAATATTTAA AAGCAATCAT ACGCTGAAAA TTTAGTGCTG TGGCTAATCC
  901 TTCAACATGG AAAATGCCAT AAAAGTGACT TTGACAAAAA AAAAAAGTAT ATACAGGTAG
  961 TAACTTCATC TACTCATGAC TTTTGTTTAC AGCATGTGGA AGAAGATATT TATTGCTAAT
 1021 CGTTAGGTTT ACCATCCATT AGTTATAACT ATTGATCGGA CAAGATTTGA CGCATGATGA
1081 AAACAGCATT AGCTTTCGGA GAAAATTTAG GTC
//
Primer: f1ori_R3
Sequence file: RDB14496_A6L7b.seq check
>06582_14496_A6L7_f1ori_R3_A08_02_ABI24.ab1
    1 GCAGGGCGGC ATTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
  241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
  301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
  361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
  421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
  481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
  541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
  601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
  661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
  721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
  781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG
  841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT
  901 TTATAAAGGC TCATGGCGTT AAAGAGGATA GTAAAAAATT CTACAATTTT TTTAAATTTA
  961 ATGTCACAAT TTGTCTTTGC GAAATATAAT TTCTATTTAA TTTTCGCTAC TGCTGTTTTA
 1021 GTACTATTAA CTTTCAATAT GCCCTTATAT GCAACTTTAA TCATGCAGAT GCAATATTTT
1081 GCATCCTTCT GGTACTGAAG CGCTTTTTTT AATCATTTTA G
//
Primer: LEU2pro_F
Sequence file: RDB14496_A6L7c.seq check
>06582_14496_A6L7_LEU2pro_F_H07_22_ABI24.ab1
    1 AATTTTGCAG TTGAAATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
   61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
  901 AGCCTCCGTT ATCCCAGGTT CCTTGGGTTT GTTGCCATCT GCGTCCTTGG GCCTCTTTGC
  961 CAGACAAGAC ACCGCATTTT GGGTTTGTAC GAACCAATGC ACGATTCTGC CTCCAGATTT
 1021 GCCAAAGAAT ACGTCCACCC CTATCGCAAC TATCCTGTCT GCTGCCATTG AATGTGGAAA
1081 TGTCATTGAA CTGGCTGAAG AGGTAGGGCC ATTTGGAAG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]