icon below as well as clone information before placing your order.
S. pombe expression vector with nmt1 promoter
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Other clones in our bank
External Database Reference sequence |
| Sequence | RDB06582z.seq |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB06582 | pHFF81 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pHFF81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06582). |
Reference section:
| Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB14496_A6L7p1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: nmt1Fw Sequence file: RDB14496_A6L7a.seq
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>06582_14496_A6L7_nmt1Fw_G07_19_ABI24.ab1 1 AGCATTTTTA TTAATCAGGA AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA 61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC 121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT 181 GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA ATTAATTATT TCAATCTCAT 241 TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG TCGACAGGCC TGGATCCTAT 301 GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA 361 GGACGACGAT GACAAAGATA TCATGAGATC TCGAGCCCGG GCGGCCGCTA ACTAACTAAG 421 GGTAAAAGGA ATGTCTCCCT TGCCAGTACT GCTAGGGTTT TTCTTTCAAA CTATGGAAGC 481 CCATTCAAGC TGCATATTAC GATTTTGTTT TTCGCTTTTA GAAAGTGGTT TAGATGAGAT 541 AATAGAAAAA TTCTTGATCT CCGACAACGA GTACTTTTAT TTTTTTTGCT AATCACTTTA 601 CTCAATATTA GCTCGAAATC GTAGAAACGT AGACGGGTGC GGGATACCGA GTGGTGTCCT 661 AAGAATGTTT TATAAACCAC GTGGCCCACA AATATGAACC CAAAACGTTT ATACATGAGT 721 ATACTTTAAG AAGGCTATAC CCCTTCGTGT TAGATGTAGT TTTAGCTACC CAACCCGAGT 781 CTATGAGCTT GACTTCAGAT GTAGAAAGCA TTAAATCGTT TTGAATATTA ATTAAAAAAC 841 GATGAAAATT AAATATTTAA AAGCAATCAT ACGCTGAAAA TTTAGTGCTG TGGCTAATCC 901 TTCAACATGG AAAATGCCAT AAAAGTGACT TTGACAAAAA AAAAAAGTAT ATACAGGTAG 961 TAACTTCATC TACTCATGAC TTTTGTTTAC AGCATGTGGA AGAAGATATT TATTGCTAAT 1021 CGTTAGGTTT ACCATCCATT AGTTATAACT ATTGATCGGA CAAGATTTGA CGCATGATGA 1081 AAACAGCATT AGCTTTCGGA GAAAATTTAG GTC // |
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Primer: f1ori_R3 Sequence file: RDB14496_A6L7b.seq
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>06582_14496_A6L7_f1ori_R3_A08_02_ABI24.ab1 1 GCAGGGCGGC ATTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG 61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC 121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT 181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC 241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA 301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT 361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT 421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA 481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG 541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA 601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT 661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA 721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA 781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT AATAACACAG 841 GTTTTGATAA ATTGTAAACT CTTACTCAAA AAAAGAATGT TAAAACTAAT TTAACCTTTT 901 TTATAAAGGC TCATGGCGTT AAAGAGGATA GTAAAAAATT CTACAATTTT TTTAAATTTA 961 ATGTCACAAT TTGTCTTTGC GAAATATAAT TTCTATTTAA TTTTCGCTAC TGCTGTTTTA 1021 GTACTATTAA CTTTCAATAT GCCCTTATAT GCAACTTTAA TCATGCAGAT GCAATATTTT 1081 GCATCCTTCT GGTACTGAAG CGCTTTTTTT AATCATTTTA G // |
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Primer: LEU2pro_F Sequence file: RDB14496_A6L7c.seq
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>06582_14496_A6L7_LEU2pro_F_H07_22_ABI24.ab1 1 AATTTTGCAG TTGAAATATT ATTTAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT 61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA 121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT 181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC 241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT 301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT 361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA 421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT 481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC 541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC 601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT 661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT 721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT 781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA 841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA 901 AGCCTCCGTT ATCCCAGGTT CCTTGGGTTT GTTGCCATCT GCGTCCTTGG GCCTCTTTGC 961 CAGACAAGAC ACCGCATTTT GGGTTTGTAC GAACCAATGC ACGATTCTGC CTCCAGATTT 1021 GCCAAAGAAT ACGTCCACCC CTATCGCAAC TATCCTGTCT GCTGCCATTG AATGTGGAAA 1081 TGTCATTGAA CTGGCTGAAG AGGTAGGGCC ATTTGGAAG // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP] |
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2023.08.18
GNP_filter3_RDBDEP_html_230702.pl