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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHFF41

S. pombe expression vector with nmt1 promoter

Catalog number RDB06581
Resource name pHFF41
Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、 寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06581 pHFF41 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06581z.seq

            Original reference

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14495_A6L7p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB14495_A6L7a.seq check
            >06581_14495_A6L7_nmt1Fw_D07_10_ABI24.ab1
                1 AGCATTTTAT TAAATCAGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
               61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
              121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
              181 AAGGAAGAGG AATCCTGGCA TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC
              241 ATTCTCACTT TCTGACTTAT AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCT
              301 ATGCATCACC ACCATCATCA TATGGATTAT AAGGATGATG ACGATAAACC CATGGACTAC
              361 AAGGACGACG ATGACAAAGA TATCATGAGA TCTCGAGCCC GGGCGGCCGC TAACTAACTA
              421 AGGGTAAAAG GAATGTCTCC CTTGCCAGTA CTGCTAGGGT TTTTCTTTCA AACTATGGAA
              481 GCCCATTCAA GCTGCATATT ACGATTTTGT TTTTCGCTTT TAGAAAGTGG TTTAGATGAG
              541 ATAATAGAAA AATTCTTGAT CTCCGACAAC GAGTACTTTT ATTTTTTTTG CTAATCACTT
              601 TACTCAATAT TAGCTCGAAA TCGTAGAAAC GTAGACGGGT GCGGGATACC GAGTGGTGTA
              661 GTTAAGAATT TTTATAAACC ACGTGGCCCA AAAATATGAA CCCAAAACGT TTATACATGA
              721 GTATACTTTA AGAAGGCTAT ACCCCTTCGT GTTAGATGTA GTTTTAGCTA CCCAACCCGA
              781 GTCTATGAGC TTGACTTCAG ATGTAGAAGG CATTAAATCG TTTTGAATAT TAATTAAAAA
              841 ACGATGAAAA TTAAATATTT AAAAGCAATC ATACGCTGAA AATTTAGTGC TGTGGCTAAT
              901 CCTTCAACAT GGAAATGCCA TAAAAGTGAC TTTGACAAAA AAAAAAAAGT ATATACAGGG
              961 TAGTAAACTC ATCTACTTCA TTGACTTTGT TTACAGCATG TGCATGAGAT ATTTATTGCT
             1021 AATCGTTAGT TACATTCATA AGTAATACTA ATGAAATCGA CAGATTGACG CATGCATGCA
            1081 AGGAGCAATT TTGCTTGTGG GGGAGAAA
            //
            Primer: f1ori_R3
            Sequence file: RDB14495_A6L7b.seq check
            >06581_14495_A6L7_f1ori_R3_F07_16_ABI24.ab1
                1 GCCAATGGCG CCTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
               61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
              121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
              181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCCAACCTTC
              241 CAATTCATTA AATCGAGTCC TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA
              301 GTAAGAGAAG TTGTTAGTGA TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT
              361 CTCTTCTCCA TTGTTTCCTT TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT
              421 TTTCTGATTA TTTTTTTCTA TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA
              481 AAATCGGGGT ATGGTTAGTA GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG
              541 GGGCAAGCGA GACAGAATGG GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA
              601 TTTTAATTTG TATTTTTTGT AATTTTATTT CTCATTGTTT TACTTAAAAT GAATGCGAAT
              661 TAGAGAAAAC TTCAACGAAA TGTCAAAATA AGCTCAGCAA AATATACAAT TTTAGGGAAA
              721 GCGATCAGCA ACTTATTCTC CGTTGTAGAT TCTTTTTCTT CATAAATTCC AACAATGTAA
              781 CTGCCAATTG ATTTTTCTCT AGATGCTAAT AAATTGAAAC TAATGAATAT ATACACAGGT
              841 TTTGATAAAT TGTAACTTCT TACTCAAAAA AAGATGTTAA AACTAATTTA ACCTTTTTTA
              901 TAAAGGCTCA TGGCGTTAAA GAGGATAGTA AAAATTCTAC ATTTTTATTT ATGTCACATT
              961 GTCTTGCAAT ATAATTCTAT ATTTCGCTAC TGCTGTTTAG TACTATTACT TACAATGGCT
             1021 TAATGGCACT TGATTCATGA GATGCCAATT TGCACTTTCT TGCACTGAGC CTTCTACCGT
            1081 TAGGATC
            //
            Primer: LEU2pro_F
            Sequence file: RDB14495_A6L7c.seq check
            >06581_14495_A6L7_LEU2pro_F_E07_13_ABI24.ab1
                1 AAACCCTGAG TGACATATTA TTTAGGACCT ATTGTTTTTT CCAATAGGTG GTTAGCAATC
               61 GTCTTACTTT CTAACTTTTC TTACCTTTTA CATTTCAGCA ATATATATAT ATATTTCAAG
              121 GATATACCAT TCTAATGTCT GCCCCTAAGA AGATCGTCGT TTTGCCAGGT GACCACGTTG
              181 GTCAAGAAAT CACAGCCGAA GCCATTAAGG TTCTTAAAGC TATTTCTGAT GTTCGTTCCA
              241 ATGTCAAGTT CGATTTCGAA AATCATTTAA TTGGTGGTGC TGCTATCGAT GCTACAGGTG
              301 TTCCACTTCC AGATGAGGCG CTGGAAGCCT CCAAGAAGGC TGATGCCGTT TTGTTAGGTG
              361 CTGTGGGTGG TCCTAAATGG GGTACCGGTA GTGTTAGACC TGAACAAGGT TTACTAAAAA
              421 TCCGTAAAGA ACTTCAATTG TACGCCAACT TAAGACCATG TAACTTTGCA TCCGACTCTC
              481 TTTTAGACTT ATCTCCAATC AAGCCACAAT TTGCTAAAGG TACTGACTTC GTTGTTGTCA
              541 GAGAATTAGT GGGAGGTATT TACTTTGGTA AGAGAAAGGA AGACGATGGT GATGGTGTCG
              601 CTTGGGATAG TGAACAATAC ACCGTTCCAG AAGTGCAAAG AATCACAAGA ATGGCCGCTT
              661 TCATGGCCCT ACAACATGAG CCACCATTGC CTATTTGGTC CTTGGATAAA GCTAATGTTT
              721 TGGCCTCTTC AAGATTATGG AGAAAAACTG TGGAGGAAAC CATCAAGAAC GAATTCCCTA
              781 CATTGAAGGT TCAACATCAA TTGATTGATT CTGCCGCCAT GATCCTAGTT AAGAACCCAA
              841 CCCACCTAAA TGGTATTATA ATCACCAGCA ACATGTTTGG TGACATCATC TCCGATGAAG
              901 CCTCCGTTAT CCCAGGTTCC TTGGGTTTGT TGCCATCTGC GTCCTTTGGC CTCTTTGCCA
              961 GACAAGAACA CCGCATTTGG GTTTGTACGA ACCATGCCAC GGTTCTGCTC AGATTTGCAA
             1021 GATACGTCAA CCCTATCGCA CTATCCTGGT CTGCTGCCAT GAATGGTGAA AATGTCATTG
            1081 ACTGGCCTGG AGAGTAAGGT CATTGAAGG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl